LOCUS       sequence-284997-        2573 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1581
FEATURES             Location/Qualifiers
     source          1..2573
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggcctc ggaggcggcg gcggcggcgg ggtccgcggc tctggcggcg
       61 ggtgcccggg ccgtcccggc ggccacgaca ggagccgccg ccgccgcctc gggcccgtgg
      121 gtgcccccgg gaccccgact gaggggcagc cggccgcggc ccgcgggggc gacgcagcag
      181 cccgctgtcc ccgcgccgcc ggcgggggag ctgatccagc cgtcggtgag cgagctgtcc
      241 cgggccgtgc ggaccaacat cctgtgcacc gtgcgcggct gcggcaagat cctgcccaac
      301 agccccgcgc tcaacatgca cctagtcaag agccaccgcc tgcaggatgg catagtcaat
      361 ccaacaataa gaaaagattt gaaaactgga ccgaaattct actgctgtcc aattgaaggc
      421 tgccccagag gccctgagag accgttttct cagttttctc tcgtaaaaca gcactttatg
      481 aaaatgcatg ctgagaagaa gcacaaatgt agtaagtgca gcaattcgta cggtacagaa
      541 tgggacctga aaagacatgc agaggactgt ggcaagacct tccggtgcac atgcggctgt
      601 ccctacgcca gtagaacagc actgcagtct cacatctacc gaactgggca cgagatacct
      661 gcagaacaca gggacccacc tagtaagaaa aggaaaatgg aaaactgtgc acaaaaccag
      721 aagttatcca acaagaccat tgaatcattg aacaaccaac caatccctag accagacact
      781 caagaactag aagcttcaga aataaagcta gaaccatctt ttgaagactc ttgtggctct
      841 aacactgaca agcagactct tacaacacca ccgagatatc ctcagaagtt gcttttacca
      901 aagcccaaag tggctttggt taaactaccc gtgatgcagt tttctgtcat gcctgtcttt
      961 gtgcctacag ccgactcctc agcccagcct gtggtgttag gtgttgatca gggctctgcc
     1021 acaggggctg tgcacttaat gcccttgtca gtaggaaccc tgatcctcgg cctagattca
     1081 gaggcttgct ctcttaagga gagcctacct cttttcaaaa ttgctaatcc tattgctggt
     1141 gagccaataa gtactggtgt tcaagtgaac tttggtaaaa gtccatctaa tcctttacaa
     1201 gaactaggga acacgtgtca aaagaatagc atttcttcaa tcaacgtgca gacagatctg
     1261 tcttatgcct cacaaaactt tataccttct gcacagtggg ccactgctga ttcctctgtg
     1321 tcgtcttgtt ctcaaactga tttgtcgttt gattctcaag tgtctcttcc cattagtgtt
     1381 cacactcaga catttttgcc cagctctaag gtaacttcat ctatagctgc tcagactgat
     1441 gcatttatgg acacctgttt ccagtcaggt ggggtctcca gagaaactca aaccagtggg
     1501 atagaaagtc caacggatga ccatgtacag atggaccaag ctggaatgtg cggagacatt
     1561 tttgagagtg ttcattcatc atataatgtt gctacaggta acattataag caacagttta
     1621 gtagcagaga cagtaactca tagtttgtta cctcagaatg agcctaagac tttaaatcaa
     1681 gatattgaga aatctgcacc aattataaat ttcagtgcac agaatagtat gcttccttca
     1741 cagaacatga cagataatca gacccaaacc atagatttat taagtgattt ggaaaacatc
     1801 ttgtcaagta atctgcctgc tcagacattg gatcatcgta gtcttttgtc tgacacaaat
     1861 cctggacctg acacccagct cccatctggc ccagcccaga accccggaat cgattttgat
     1921 atcgaagagt tcttttcggc ctcaaatatc cagactcaaa ctgaagagag tgaacttagc
     1981 accatgacca ccgagccagt cttggagtca ctggacatag agactcaaac ggacttctta
     2041 ctcgcagata cctctgctca gtcctatggg tgtaggggaa attctaactt cttaggcctt
     2101 gagatgtttg acacacagac acagacagac ttaaactttt tcttagacag tagccctcat
     2161 ctgcctctgg gaagtattct gaaacactcc agcttttccg tgagtactga ttcatctgac
     2221 acagagaccc aaactgaagg agtctccact gctaaaaata tacctgctct agaaagcaaa
     2281 gttcagttga acagtacaga aacacagacc atgagttctg ggtttgaaac cctggggagc
     2341 ttgttcttca ccagcaacga aactcagaca gcaatggatg actttcttct ggctgatctg
     2401 gcctggaaca cgatggagtc tcagttcagc tctgtagaaa cccagacttc tgcggaacca
     2461 cacacagtct ccaacttcta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     2521 tggctttata tatcttgtgg aaaggacgag catgggcccg ttcgctatgt cac
//
