LOCUS       sequence-285000-        8621 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8621)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 8621)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1618
FEATURES             Location/Qualifiers
     source          1..8621
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggccgcgca caggccggtg gaatgggtcc aggccgtggt cagccgcttc
       61 gacgagcagc ttccaataaa aacaggacag cagaacacac ataccaaagt cagtactgag
      121 cacaacaagg aatgtctaat caatatttcc aaatacaagt tttctttggt tataagcggc
      181 ctcactacta ttttaaagaa tgttaacaat atgagaatat ttggagaagc tgctgaaaaa
      241 aatttatatc tctctcagtt gattatattg gatacactgg aaaaatgtct tgctgggcaa
      301 ccaaaggaca caatgagatt agatgaaacg atgctggtca aacagttgct gccagaaatc
      361 tgccattttc ttcacacctg tcgtgaagga aaccagcatg cagctgaact tcggaattct
      421 gcctctgggg ttttattttc tctcagctgc aacaacttca atgcagtctt tagtcgcatt
      481 tctaccaggt tacaggaatt aactgtttgt tcagaagaca atgttgatgt tcatgatata
      541 gaattgttac agtatatcaa tgtggattgt gcaaaattaa aacgactcct gaaggaaaca
      601 gcatttaaat ttaaagccct aaagaaggtt gcgcagttag cagttataaa tagcctggaa
      661 aaggcatttt ggaactgggt agaaaattat ccagatgaat ttacaaaact gtaccagatc
      721 ccacagactg atatggctga atgtgcagaa aagctatttg acttggtgga tggttttgct
      781 gaaagcacca aacgtaaagc agcagtttgg ccactacaaa tcattctcct tatcttgtgt
      841 ccagaaataa tccaggatat atccaaagac gtggttgatg aaaacaacat gaataagaag
      901 ttatttctgg acagtctacg aaaagctctt gctggccatg gaggaagtag gcagctgaca
      961 gaaagtgctg caattgcctg tgtcaaactg tgtaaagcaa gtacttacat caattgggaa
     1021 gataactctg tcattttcct acttgttcag tccatggtgg ttgatcttaa gaacctgctt
     1081 tttaatccaa gtaagccatt ctcaagaggc agtcagcctg cagatgtgga tctaatgatt
     1141 gactgccttg tttcttgctt tcgtataagc cctcacaaca accaacactt taagatctgc
     1201 ctggctcaga attcaccttc tacatttcac tatgtgctgg taaattcact ccatcgaatc
     1261 atcaccaatt ccgcattgga ttggtggcct aagattgatg ctgtgtattg tcactcggtt
     1321 gaacttcgaa atatgtttgg tgaaacactt cataaagcag tgcaaggttg tggagcacac
     1381 ccagcaatac gaatggcacc gagtcttaca tttaaagaaa aagtaacaag ccttaaattt
     1441 aaagaaaaac ctacagacct ggagacaaga agctataagt atcttctctt gtccatggtg
     1501 aaactaattc atgcagatcc aaagctcttg ctttgtaatc caagaaaaca ggggcccgaa
     1561 acccaaggca gtacagcaga attaattaca gggctcgtcc aactggtccc tcagtcacac
     1621 atgccagaga ttgctcagga agcaatggag gctctgctgg ttcttcatca gttagatagc
     1681 attgatttgt ggaatcctga tgctcctgta gaaacatttt gggagattag ctcacaaatg
     1741 cttttttaca tctgcaagaa attaactagt catcaaatgc ttagtagcac agaaattctc
     1801 aagtggttgc gggaaatatt gatctgcagg aataaatttc ttcttaaaaa taagcaggca
     1861 gatagaagtt cctgtcactt tctccttttt tacggggtag gatgtgatat tccttctagt
     1921 ggaaatacca gtcaaatgtc catggatcat gaagaattac tacgtactcc tggagcctct
     1981 ctccggaagg gaaaagggaa ctcctctatg gatagtgcag caggatgcag cggaaccccc
     2041 ccgatttgcc gacaagccca gaccaaacta gaagtggccc tgtacatgtt tctgtggaac
     2101 cctgacactg aagctgttct ggttgccatg tcctgtttcc gccacctctg tgaggaagca
     2161 gatatccggt gtggggtgga tgaagtgtca gtgcataacc tcttgcccaa ctataacaca
     2221 ttcatggagt ttgcctctgt cagcaatatg atgtcaacag gaagagcagc acttcagaaa
     2281 agagtgatgg cactgctgag gcgcattgag catcccactg caggaaacac tgaggcttgg
     2341 gaagatacac atgcaaaatg ggaacaagca acaaagctaa tccttaacta tccaaaagcc
     2401 aaaatggaag atggccaggc tgctgaaagc cttcacaaga ccattgttaa gaggcgaatg
     2461 tcccatgtga gtggaggagg atccatagat ttgtctgaca cagactccct acaggaatgg
     2521 atcaacatga ctggcttcct ttgtgccctt gggggagtgt gcctccagca gagaagcaat
     2581 tctggcctgg caacctatag cccacccatg ggtccagtca gtgaacgtaa gggttctatg
     2641 atttcagtga tgtcttcaga gggaaacgca gatacacctg tcagcaaatt tatggatcgg
     2701 ctgttgtcct taatggtgtg taaccatgag aaagtgggac ttcaaatacg gaccaatgtt
     2761 aaggatctgg tgggtctaga attgagtcct gctctgtatc caatgctatt taacaaattg
     2821 aagaatacca tcagcaagtt ttttgactcc caaggacagg ttttattgac tgataccaat
     2881 actcaatttg tagaacaaac catagctata atgaagaact tgctagataa tcatactgaa
     2941 ggcagctctg aacatctagg gcaagctagc attgaaacaa tgatgttaaa tctggtcagg
     3001 tatgttcgtg tgcttgggaa tatggtccat gcaattcaaa taaaaacgaa actgtgtcaa
     3061 ttagttgaag taatgatggc aaggagagat gacctctcat tttgccaaga gatgaaattt
     3121 aggaataaga tggtagaata cctgacagac tgggttatgg gaacatcaaa ccaagcagca
     3181 gatgatgatg taaaatgtct tacaagagat ttggaccagg caagcatgga agcagtagtt
     3241 tcacttctag ctggtctccc tctgcagcct gaagaaggag atggtgtgga attgatggaa
     3301 gccaaatcac agttatttct taaatacttc acattattta tgaacctttt gaatgactgc
     3361 agtgaagttg aagatgaaag tgcgcaaaca ggtggcagga aacgtggcat gtctcggagg
     3421 ctggcatcac tgaggcactg tacggtcctt gcaatgtcaa acttactcaa tgccaacgta
     3481 gacagtggtc tcatgcactc cataggctta ggttaccaca aggatctcca gacaagagct
     3541 acatttatgg aagttctgac aaaaatcctt caacaaggca cagaatttga cacacttgca
     3601 gaaacagtat tggctgatcg gtttgagaga ttggtggaac tggtcacaat gatgggtgat
     3661 caaggagaac tccctatagc gatggctctg gccaatgtgg ttccttgttc tcagtgggat
     3721 gaactagctc gagttctggt tactctgttt gattctcggc atttactcta ccaactgctc
     3781 tggaacatgt tttctaaaga agtagaattg gcagactcca tgcagactct cttccgaggc
     3841 aacagcttgg ccagtaaaat aatgacattc tgtttcaagg tatatggtgc tacctatcta
     3901 caaaaactcc tggatccttt attacgaatt gtgatcacat cctctgattg gcaacatgtt
     3961 agctttgaag tggatcctac caggttagaa ccatcagaga gccttgagga aaaccagcgg
     4021 aacctccttc agatgactga aaagttcttc catgccatca tcagttcctc ctcagaattc
     4081 ccccctcaac ttcgaagtgt gtgccactgt ttataccagg caacttgcca ctccctactg
     4141 aataaagcta cagtaaaaga aaaaaaggaa aacaaaaaat cagtggttag ccagcgtttc
     4201 cctcagaaca gcatcggtgc agtaggaagt gccatgttcc tcagatttat caatcctgcc
     4261 attgtctcac cgtatgaagc agggatttta gataaaaagc caccacctag aatcgaaagg
     4321 ggcttgaagt taatgtcaaa gatacttcag agtattgcca atcatgttct cttcacaaaa
     4381 gaagaacata tgcggccttt caatgatttt gtgaaaagca actttgatgc agcacgcagg
     4441 tttttccttg atatagcatc tgattgtcct acaagtgatg cagtaaatca tagtctttcc
     4501 ttcataagtg acggcaatgt gcttgcttta catcgtctac tctggaacaa tcaggagaaa
     4561 attgggcagt atctttccag caacagggat cataaagctg ttggaagacg accttttgat
     4621 aagatggcaa cacttcttgc atacctgggt cctccagagc acaaacctgt ggcagataca
     4681 cactggtcca gccttaacct taccagttca aagtttgagg aatttatgac taggcatcag
     4741 gtacatgaaa aagaagaatt caaggctttg aaaacgttaa gtattttcta ccaagctggg
     4801 acttccaaag ctgggaatcc tattttttat tatgttgcac ggaggttcaa aactggtcaa
     4861 atcaatggtg atttgctgat ataccatgtc ttactgactt taaagccata ttatgcaaag
     4921 ccatatgaaa ttgtagtgga ccttacccat accgggccta gcaatcgctt taaaacagac
     4981 tttctctcta agtggtttgt tgtttttcct ggctttgctt acgacaacgt ctccgcagtc
     5041 tatatctata actgtaactc ctgggtcagg gagtacacca agtatcatga gcggctgctg
     5101 actggcctca aaggtagcaa aaggcttgtt ttcatagact gtcctgggaa actggctgag
     5161 cacatagagc atgaacaaca gaaactacct gctgccacct tggctttaga agaggacctg
     5221 aaggtattcc acaatgctct caagctagct cacaaagaca ccaaagtttc tattaaagtt
     5281 ggttctactg ctgtccaagt aacttcagca gagcgaacaa aagtcctagg gcaatcagtc
     5341 tttctaaatg acatttatta tgcttcggaa attgaagaaa tctgcctagt agatgagaac
     5401 cagttcacct taaccattgc aaaccagggc acgccgctca ccttcatgca ccaggagtgt
     5461 gaagccattg tccagtctat cattcatatc cggacccgct gggaactgtc acagcccgac
     5521 tctatccccc aacacaccaa gattcggcca aaagatgtcc ctgggacact gctcaatatc
     5581 gcattactta atttaggcag ttctgacccg agtttacggt cagctgccta taatcttctg
     5641 tgtgccttaa cttgtacctt taatttaaaa atcgagggcc agttactaga gacatcaggt
     5701 ttatgtatcc ctgccaacaa caccctcttt attgtctcta ttagtaagac actggcagcc
     5761 aatgagccac acctcacgtt agaatttttg gaagagtgta tttctggatt tagcaaatct
     5821 agtattgaat tgaaacacct ttgtttggaa tacatgactc catggctgtc aaatctagtt
     5881 cgtttttgca agcataatga tgatgccaaa cgacaaagag ttactgctat tcttgacaag
     5941 ctgataacaa tgaccatcaa tgaaaaacag atgtacccat ctattcaagc aaaaatatgg
     6001 ggaagccttg ggcagattac agatctgctt gatgttgtac tagacagttt catcaaaacc
     6061 agtgcaacag gtggcttggg atcaataaaa gctgaggtga tggcagatac tgctgtagct
     6121 ttggcttctg gaaatgtgaa attggtttca agcaaggtta ttggaaggat gtgcaaaata
     6181 attgacaaga catgcttatc tccaactcct actttagaac aacatcttat gtgggatgat
     6241 attgctattt tagcacgcta catgctgatg ctgtccttca acaattccct tgatgtggca
     6301 gctcatcttc cctacctctt ccacgttgtt actttcttag tagccacagg tccgctctcc
     6361 cttagagctt ccacacatgg actggtcatt aatatcattc actctctgtg tacttgttca
     6421 cagcttcatt ttagtgaaga gaccaagcaa gttttgagac tcagtctgac agagttctca
     6481 ttacccaaat tttacttgct gtttggcatt agcaaagtca agtcagctgc tgtcattgcc
     6541 ttccgttcca gttaccggga caggtcattc tctcctggct cctatgagag agagactttt
     6601 gctttgacat ccttggaaac agtcacagaa gctttgttgg agatcatgga ggcatgcatg
     6661 agagatattc caacgtgcaa gtggctggac cagtggacag aactagctca aagatttgca
     6721 ttccaatata atccatccct gcaaccaaga gctcttgttg tctttgggtg tattagcaaa
     6781 cgagtgtctc atgggcagat aaagcagata atccgtattc ttagcaaggc acttgagagt
     6841 tgcttaaaag gacctgacac ttacaacagt caagttctga tagaagctac agtaatagca
     6901 ctaaccaaat tacagccact tcttaataag gactcgcctc tgcacaaagc cctcttttgg
     6961 gtagctgtgg ctgtgctgca gcttgatgag gtcaacttgt attcagcagg taccgcactt
     7021 cttgaacaaa acctgcatac tttagatagt ctccgtatat tcaatgacaa gagtccagag
     7081 gaagtattta tggcaatccg gaatcctctg gagtggcact gcaagcaaat ggatcatttt
     7141 gttggactca atttcaactc taactttaac tttgcattgg ttggacacct tttaaaaggg
     7201 tacaggcatc cttcacctgc tattgttgca agaacagtca gaattttaca tacactacta
     7261 actctggtta acaaacacag aaattgtgac aaatttgaag tgaatacaca gagcgtggcc
     7321 tacttagcag ctttacttac agtgtctgaa gaagttcgaa gtcgctgcag cctaaaacat
     7381 agaaagtcac ttcttcttac tgatatttca atggaaaatg ttcctatgga tacatatccc
     7441 attcatcatg gtgacccttc ctataggaca ctaaaggaga ctcagccatg gtcctctccc
     7501 aaaggttctg aaggatacct tgcagccacc tatccaactg tcggccagac cagtccccga
     7561 gccaggaaat ccatgagcct ggacatgggg caaccttctc aggccaacac taagaagttg
     7621 cttggaacaa ggaaaagttt tgatcacttg atatcagaca caaaggctcc taaaaggcaa
     7681 gaaatggaat cagggatcac aacacccccc aaaatgagga gagtagcaga aactgattat
     7741 gaaatggaaa ctcagaggat ttcctcatca caacagcacc cacatttacg taaagtttca
     7801 gtgtctgaat caaatgttct cttggatgaa gaagtactta ctgatccgaa gatccaggcg
     7861 ctgcttctta ctgttctagc tacactggta aaatatacca cagatgagtt tgatcaacga
     7921 attctttatg aatacttagc agaggccagt gttgtgtttc ccaaagtctt tcctgttgtg
     7981 cataatttgt tggactctaa gatcaacacc ctgttatcat tgtgccaaga tccaaatttg
     8041 ttaaatccaa tccatggaat tgtgcagagt gtggtgtacc atgaagaatc cccaccacaa
     8101 taccaaacat cttacctgca aagttttggt tttaatggct tgtggcggtt tgcaggaccg
     8161 ttttcaaagc aaacacaaat tccagactat gctgagctta ttgttaagtt tcttgatgcc
     8221 ttgattgaca cgtacctgcc tggaattgat gaagaaacca gtgaagaatc cctcctgact
     8281 cccacatctc cttaccctcc tgcactgcag agccagctta gtatcactgc caaccttaac
     8341 ctttctaatt ccatgacctc acttgcaact tcccagcatt ccccaggaat cgacaaggag
     8401 aacgttgaac tctcccctac cactggccac tgtaacagtg gacgaactcg ccacggatcc
     8461 gcaagccaag tgcagaagca aagaagcgct ggcagtttca aacgtaatag cattaagaag
     8521 atcgtgtagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     8581 tcttgtggaa aggacgacgc aacattgagg gtagtccatc c
//
