LOCUS       sequence-285006-        3593 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3593)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1624
FEATURES             Location/Qualifiers
     source          1..3593
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggatcgcaa cagagaggcc gagatggagc tgaggcgagg ccccagcccc
       61 accagggccg gccggggcca cgaggtggat ggggacaagg ctacctgcca cacctgctgc
      121 atctgcggca agagcttccc cttccagagc tcgctttcgc agcacatgcg caagcacacg
      181 ggcgagaagc cctacaagtg tccctactgc gaccaccggg cttcccagaa gggcaacctg
      241 aagattcaca tccggagcca ccgcacgggg actctgattc agggacacga gccggaggcg
      301 ggcgaggcgc cgctgggtga gatgcgcgcc tccgagggcc tggacgcctg cgccagcccc
      361 accaagagcg cctcggcctg caaccggctg ctgaacgggg cctcgcaggc cgacggcgcc
      421 agggtcctga acggggcctc gcaggccgac agcggcagag tcctgctgcg gagcagcaag
      481 aagggggcag aggggtccgc atgcgccccg ggggaggcca aggcagcggt ccagtgctcc
      541 ttctgcaaga gccagttcga gcgtaagaag gacctggagc tgcacgtgca ccaggcgcac
      601 aagccgttca agtgcaggct gtgcagctac gcgacgctgc gggaggagtc gctgctgagc
      661 cacatcgaga gggaccacat caccgcgcag gggcccggca gcggcgaggc ctgcgtggag
      721 aacggcaagc ccgagctgag ccccggggag ttcccgtgcg aggtgtgtgg ccaggccttc
      781 agccagacct ggttcctgaa ggcgcacatg aagaagcacc ggggctcctt cgaccacggc
      841 tgccacatct gcggccgtag gttcaaggag ccctggttcc tcaagaacca catgaaggcg
      901 cacggcccca agacgggcag caagaacagg cccaagagtg agctggaccc catcgccacc
      961 atcaacaacg tggtccagga ggaggtgatc gtcgccggcc tgagcctcta cgaggtctgc
     1021 gccaagtgcg ggaacctgtt tacaaacctg gacagcttga acgcccacaa tgccatccac
     1081 cgcagagtcg aggccagccg cacgcgcgcc ccggccgagg agggggcgga ggggccctcg
     1141 gacaccaagc agttcttcct ccagtgcctg aacctgaggc cgtcggcggc cggcgactcg
     1201 tgccctggca cgcaggccgg acggcgggtg gctgagctgg acccggtcaa cagctaccag
     1261 gcctggcagc tggccacgcg gggtaaggtg gccgagccgg ccgagtacct caagtacggg
     1321 gcctgggacg aggcgctggc cggggacgtg gccttcgaca aggacaggcg cgagtacgtc
     1381 ctggtgagcc aggagaagcg caagcgtgag caggatgcac cagccgcgca ggggcccccg
     1441 cggaagcgcg cgagcgggcc tggggacccc gcgcccgccg gccacctcga tccccgctcg
     1501 gccgcgcgcc ccaaccgcag ggccgcagcc accaccggcc agggcaagtc ctccgagtgc
     1561 ttcgagtgcg gcaagatctt ccgcacctat catcagatgg tgctgcactc acgcgtgcat
     1621 cgccgcgcgc gccgcgagag ggacagtgac ggggacaggg cggcgcgggc ccgctgcgga
     1681 tcactcagtg agggtgactc ggcctcccag cccagcagcc ctggctccgc ctgtgccgct
     1741 gctgactccc cgggctctgg cctggccgac gaggctgccg aagacagtgg tgaggagggc
     1801 gcccctgaac ctgcaccagg gggacagccg cgccgctgct gcttttccga agaggtgact
     1861 tcgaccgagc tctccagtgg agaccagagt cacaagatgg gagataacgc ctcggaaaga
     1921 gacaccggcg agtccaaggc agggatcgca gcttctgtgt ccatacttga aaacagtagc
     1981 agagagactt ctagaaggca agagcagcac agattttcta tggacttaaa gatgccagca
     2041 tttcacccca agcaggaggt gcccgtccct ggtgatggtg tggagttccc ttccagtacg
     2101 ggagcggagg gccagacggg tcaccctgca gaaaagctgt ccgatttgca caacaaggaa
     2161 cactctgggg gagggaagcg ggcgctggcc ccagacctca tgccgctaga tttaagtgcg
     2221 aggtcgacgc gggatgaccc cagcaataag gagacggcct cctccctgca ggcggcttta
     2281 gtcgttcacc cgtgtcctta ctgcagccac aagacctact accccgaggt cctgtggatg
     2341 cacaaacgca tctggcaccg cgtcagctgc aactccgtgg ctcccccgtg gattcagccc
     2401 aatggttaca aaagcatcag aagcaatttg gttttccttt cccggagcgg acgcacgggc
     2461 cccccgcctg ccctcggtgg caaagaatgc cagcctttgc tccttgctcg gttcacccgc
     2521 actcaggtgc caggggggat gccggggtcc aaaagtggct cttctcccct gggagtggtc
     2581 acaaaagccg ctagcatgcc taagaataag gagagccatt ccggaggtcc ctgcgctctg
     2641 tgggcgcccg gccctgacgg gtatcgacag accaaacctt gtcacggcca ggagccacat
     2701 ggcgcggcca cacaggggcc cctggccaag cccaggcagg aggctagctc caaaccggtg
     2761 cctgccccgg gtggcggggg cttcagcagg agcgccaccc ctacgcccac cgtcatcgcc
     2821 cgggctggcg cgcagccctc ggccaatagc aagcctgtgg agaagtttgg ggtcccccca
     2881 gcgggggctg gctttgcccc cacaaataag cacagtgccc cggactccct gaaagccaaa
     2941 ttcagtgctc agcctcaggg tccacctcct gcaaagggcg aagggggcgc tcctcctcta
     3001 cctccccgcg agcccccctc gaaggcagcc caggagctga ggactctggc cacctgtgct
     3061 gcggggtcca ggggcgacgc ggccttgcag gcccagcccg gcgtggctgg ggcgcccccc
     3121 gtcctacact ccatcaaaca ggagccggtg gccgaggggc atgagaagcg cctggacatc
     3181 ctcaacatct ttaagacgta cattccaaag gactttgcga ccctctacca gggatggggt
     3241 gtcagcggcc ctgggttgga gcacagaggg acactccgga cgcaggcccg gccaggagag
     3301 ttcgtctgca tcgagtgcgg aaagagcttc caccagcccg gccacctcag ggcccacatg
     3361 cgggcacact cagtggtgtt tgagtccgat gggcctcggg gttctgaagt tcataccacc
     3421 tccgcagacg cccccaaaca agggagagac cattctaaca caggtaccgt ccagacagtg
     3481 cctctgagaa agggaaccta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     3541 tggctttata tatcttgtgg aaaggacgag ccaatccaca gatatcttta acg
//
