LOCUS       sequence-285012-        2348 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1632
FEATURES             Location/Qualifiers
     source          1..2348
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggtggagaa cttcaagaac ctggttgcag tggggcatct tcccttccaa
       61 ccagatatgg tatcccaatt ggaagcagaa gaaaagcttt ggatgatgga aacagaaacc
      121 caaagaagca gcaagcatca aaataagatg gaaacactcc aaaaatttgc attaaaatac
      181 ctttcaaatc aagagctgtc ctgctggcaa atctggaaac aggttgcaag tgaattaacc
      241 aggtgtcttc aggggaagag ttcccagtta ttacaaggtg actctattca ggtttctgaa
      301 aatgagaaca atataatgaa ccctaaagga gatagctcta tttatattga aaatcaagag
      361 tttccatttt ggagaaccca gcattcttgc gggaatacat atctgagtga gtcacagatt
      421 cagagtagag gtaagcaaat tgatgtgaaa aataacctgc aaatacatga agacttcatg
      481 aagaaatcac catttcatga gcatattaaa actgacacag aaccaaaacc ctgcaaaggt
      541 aatgaatatg gcaaaatcat tagtgatggc tccaaccaga aattaccctt aggagagaaa
      601 ccccatccat gtggtgagtg tggaaggggc ttcagttata gcccaaggct tccccttcat
      661 ccgaatgttc atacaggaga aaaatgcttc agtcaaagct cacatctgcg aactcatcag
      721 agaattcacc caggagagaa actcaataga tgtcatgaat ctggtgattg cttcaataag
      781 agctcttttc attcttatca atctaatcat acaggagaga agtcttatag atgcgacagt
      841 tgcggcaagg gattcagtag cagcacgggt cttatcattc attacagaac tcatactgga
      901 gagaaaccct ataaatgcga ggaatgtggt aaatgcttta gtcaaagttc aaattttcag
      961 tgccatcaga gagtccacac tgaagaaaaa ccatacaaat gcgaagagtg tggtaagggc
     1021 ttcggttgga gtgttaatct ccgtgttcac cagagggtcc acaggggtga gaagccctat
     1081 aaatgtgagg aatgtggtaa gggcttcact caggctgcac attttcacat ccatcagaga
     1141 gtccacactg gagagaaacc ctacaagtgt gatgtgtgtg gtaagggctt cagccacaat
     1201 tcaccattaa tatgccatcg gagagtccac acaggagaga agccatacaa gtgtgaggcg
     1261 tgtgggaaag gctttacccg taatacagat ctgcatattc atttcagagt tcacacggga
     1321 gagaaaccct ataaatgtaa ggagtgtggt aagggcttca gtcaggcttc aaatcttcaa
     1381 gtccatcaga atgtccacac tggggagaaa cgattcaagt gtgaaacgtg tgggaagggc
     1441 ttcagtcagt cctcaaagct tcaaacccat cagcgagtcc acactggaga gaaaccatat
     1501 agatgtgatg tgtgtggtaa ggacttcagt tatagttcaa atcttaaact acaccaagta
     1561 attcacactg gagaaaaacc atataaatgt gaggaatgtg ggaagggctt cagttggaga
     1621 tcaaatcttc atgcacatca aagagttcac tcaggagaaa aaccctataa atgtgagcag
     1681 tgtgataaga gcttcagtca ggccatagat tttcgggtac atcagagagt ccatactgga
     1741 gagaagccat acaaatgtgg tgtctgtggt aagggcttca gtcagtcctc tggtcttcaa
     1801 tcccatcaga gagtccacac gggggaaaag ccatacaaat gtgatgtgtg tggaaagggc
     1861 tttagataca gttcgcagtt tatataccat cagagaggcc acactggaga aaaaccttac
     1921 aaatgtgaag agtgtgggaa aggctttggt aggagcttga atcttcgcca tcatcagagg
     1981 gtccacacgg gagagaaacc ccatatatgt gaggagtgtg gtaaggcctt cagtctcccc
     2041 tcaaatcttc gagtccacct gggtgttcac accagggaaa aactctttaa atgtgaagag
     2101 tgtggtaaag gcttcagtca gagtgcacgt cttgaagccc atcagagagt ccacactgga
     2161 gaaaaaccat acaaatgtga catatgtgat aaggacttcc gtcaccgttc acgtcttaca
     2221 tatcatcaga aagtccatac tggtaaaaag ctttagtaat gaactagtcc gtaacttgaa
     2281 agtatttcga tttcttggct ttatatatct tgtggaaagg acgatggaaa gcatcgaata
     2341 tatggcta
//
