LOCUS       sequence-285015-        2561 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1633
FEATURES             Location/Qualifiers
     source          1..2561
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(1404..1415)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     RBS             1832..1840
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 accgccacca tggagacttt gacctcaagg catgagaaaa gagctcttca ttctcaagcc
       61 tcagccattt cccaagatag ggaggagaag atcatgtctc aggagccatt gagcttcaag
      121 gacgtggctg tggtcttcac tgaggaggag ctagagctgc tggactctac ccagaggcag
      181 ctgtaccaag atgtgatgca ggagaatttc aggaacctac tctcagtggg agacaagaat
      241 ggaaaggata cggagtatat tcaagatgaa gaattaaggt tcttttcaca caaagagctc
      301 tcctcatgca aaatctggga agaggtggca ggtgaattac ctgggagcca agactgtaga
      361 gtaaatctgc aaggaaaaga cttccagttc tcagaagatg ctgctcccca tcaagggtgg
      421 gaaggagcat ctacgccgtg ttttccaatt gagaatttcc tggacagtct acaaggggat
      481 gggcttatcg gtctagaaaa tcaacagttt ccagcctgga gagctataag accaatcccc
      541 attcaaggat cttgggcaaa agcgtttgtg aaccagttag gggatgttca agaaagatgt
      601 aaaaatctcg acacagaaga cacagtatat aaatgtaact gggatgatga cagcttttgc
      661 tggatatctt gtcatgttga tcacagattc cctgaaatag acaagccgtg tggttgcaat
      721 aaatgcagaa aagactgcat taaaaactct gtacttcatc gcattaaccc tggagagaat
      781 ggcttgaaaa gtaacgaata cagaaatggc ttcagggacg atgcagacct tcccccgcat
      841 ccaagagtac ctttgaaaga gaaactctgt caatatgatg agtttagtga gggcttgagg
      901 cacagtgccc atcttaacag acatcaaaga gttcccacag gagagaaatc tgttaagagt
      961 cttgagcgtg gtcggggcgt cagacagaac acgcacatac gtaaccaccc cagagcccct
     1021 gtgggagaca tgccctatag atgtgatgtc tgtggaaagg ggttcaggta taaatcggtt
     1081 cttcttattc atcaaggggt gcacacagga aggagaccct ataaatgtga ggagtgtggg
     1141 aaggcatttg gtcgaagttc aaacctgctt gtccatcaga gggtccacac tggagagaaa
     1201 ccatataaat gcagcgagtg tgggaagggc ttcagttaca gctcagtgct tcaagtccat
     1261 cagaggctgc acacagggga gaagccctac acctgcagcg agtgtggcaa aggcttctgt
     1321 gccaagtctg cactgcacaa acaccagcac attcaccctg gagaaaagcc ctacagctgt
     1381 ggcgagtgtg gaaagggatt cagctgcagc tcccacctca gcagtcatca gaagacacac
     1441 accggcgaga ggccctacca gtgtgacaag tgtggcaaag gtttcagtca caactcgtac
     1501 cttcaagctc accagagagt tcacatgggg cagcatctgt acaaatgtaa cgtgtgtggt
     1561 aagagtttca gttacagctc agggcttctc atgcatcaga gactgcacac aggagagaaa
     1621 ccctacaaat gcgagtgcgg gaagagcttt ggccggagct ccgacctcca catccatcag
     1681 agggtccaca caggagagaa accctataaa tgcagtgagt gtgggaaggg cttccggcgg
     1741 aattcagacc ttcacagcca ccagagggtc cacacgggag agaggcccta cgtgtgtgac
     1801 gtgtgtggga agggtttcat ctacagctcc gacctcctta tccatcagag ggtccacact
     1861 ggagagaaac cctataaatg tgctgagtgt ggcaaaggct tcagttacag ctcagggctt
     1921 ctcattcacc agagagtcca cacaggcgag aaaccttaca gatgccaaga gtgcggaaag
     1981 ggctttaggt gcacatcaag ccttcacaaa catcagcgag tccacacggg aaaaaagccc
     2041 tatacgtgtg atcagtgtgg caagggattc agttatggct ctaatcttcg cacccaccag
     2101 aggttgcaca caggagagaa accctacact tgttgtgaat gtgggaaggg tttcagatat
     2161 ggctcaggtc tccttagtca taagagagtg cacactggcg agaagccata cagatgccac
     2221 gtgtgtggga agggctatag tcagagctca catcttcaag gtcatcagag ggtccacact
     2281 ggtgagaaac cctataaatg tgaggagtgt gggaagggct ttggccgcaa ctcctgtctt
     2341 catgttcatc agagagtcca cactggagag aagccctata cgtgtggtgt gtgtgggaaa
     2401 ggcttcagtt atacctcagg tctgcggaac caccaaagag tgcatttagg cgagaaccct
     2461 tataagtagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     2521 tcttgtggaa aggacgaacc atccactttg tttgtcgcga a
//
