LOCUS       sequence-285018-        2126 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1648
FEATURES             Location/Qualifiers
     source          1..2126
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggaccatt gacatttaca gatgtggcca tagaattctc tctggaggag
       61 tggcagtgcc tggacacagc acaacagaac ttatatagaa atgtgatgtt agagaactac
      121 agaaacttgg tcttcctggg tattgctgtt tctaagccag acctaatcac ttgtctagag
      181 aaagaaaaag aaccctgcaa gatgaagcga catgaaatgg tggatgaacc cccagttgtg
      241 tgttctcatt ttgctgaaga cttttggcca gagcaagaca taaaagattc tttccaaaaa
      301 gtgacactga ggagatatga taaacgtgga catgagaact tacaattaag aaaaggctat
      361 aaaactgtag gtgattgtaa gctatacaaa ggaggttata atggacttaa ccaatgtttg
      421 acacttaccc agagcaaaat gtatcactgt gatatatatg taaaagtctt ttatgcattt
      481 tcaaatgcag atagatacaa gacaagacat actggaaaga aacctttcca gtgtaaaaaa
      541 tgtggcaaat cattttgcat gctttcacaa ctaactcaac ataagaaaat tcatattaga
      601 gagaatacct acagatgtaa agaatttggc aatgccttta atcagtcctc agcccttact
      661 aaccataaga gaatttatgt tggtgagaaa cactacagat gtgaagaatg tggcaaagca
      721 tttaaccact actcaaccct tactaaccat aagagaattc atactggaga gaaaccctac
      781 aaatgtaaag aatgtggcaa agcctttagc aggtactcaa cccttactac ccataagaga
      841 attcattctg gagagaagcc ctacaaatgt gatgaatgtg gcaaaacctt tagcatatcc
      901 tcaaccttta ctaaacataa gataattcat actgaagaga aaccctacaa atgtaaagaa
      961 tgtggcaaag cctttaaccg gtcctcaacc cttactagcc ataagagaat acatactggt
     1021 gagaaaccct acaaatgtga agaatgtggc aaagccttta actggtcttc aactcttact
     1081 aaacataagg taattcatac tggagagaag ccctacaaat gtgaagaatg tggcaaagct
     1141 tttaaccagt cttcaagact tactcgacat aaaaaaattc atactggaga ggaaccctac
     1201 aaatttgaaa aatgtggcag agtttttacc tgttcctcaa cacttactca agacaagaaa
     1261 attcatactg gagagaaacc ctacaattgt gaagaatgtg gcaaagtttt tacctattcc
     1321 tctacactta ctagacataa gagaattcat actgaagaga aaccctataa atgtaacgaa
     1381 tgtggcaaag cttttaaccg gtcctcacac cttactagcc ataggagaat tcatactgga
     1441 gagaaaccct acaaatgtga agaatgtggc aaagccttta agcagtcctc aaaccttaac
     1501 agtcataaaa aaattcatag tggagagaaa ccctacaaat gtgaagaatg tggcaaagct
     1561 tttatcctgt cctcaagact tactcaacat aagaaaattc atactggaga gaaaccttac
     1621 aaatgtgaag aatgtggcaa agcttttaac cggtcctcaa gacttactca acataagaaa
     1681 attcatactg gagagaaacc ctacaaatgt aaacaatgtg acaaagcttt tacccactcc
     1741 tcaaacctta gtagtcataa gaaaattcat agtggagaga aaccctacaa atgtgaagaa
     1801 tgtggcaaag cttttaatcg gtcctcaaga cttactcaac ataagaaaat tcatactaga
     1861 gagaaacctt acaaatgtga agaatgtgcc aaagctttta cccggtcttc aagacttact
     1921 caacataaga aaattcatag gatgggtgtg gtggctcatg cctgtaatcc cagcactttg
     1981 ggaggcagag gtgggcggat cacgaggtca ggagatcgag accgtcctgg ctagtaatga
     2041 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     2101 gacataggcg tgagattcta cggagt
//
