LOCUS       sequence-285027-        1787 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1720
FEATURES             Location/Qualifiers
     source          1..1787
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggccgaccc gcaggctggc tccgcggccg gggactggga gatcgatgtc
       61 gagagcctgg agctggaaga ggacgtctgc ggggcgccgc ggtccacgcc ccccgggccc
      121 agcccgccgc cggcggacgg ggactgcgag gacgacgaag atgacgacgg ggtggacgaa
      181 gacgcggaag aagagggcga cggcgaggag gcaggcgcgt cccccgggat gcccggccag
      241 ccggagcagc gggggggacc gcagccgagg ccgccgctcg cgcctcaggc ctcacccgcc
      301 ggcaccggtc cccgagagcg ctgcactccc gcgggcggcg gcgcggagcc gcgcaagctg
      361 agccgcacgc ccaagtgcgc gcgctgccgc aaccacggcg tggtgtcctg cctgaagggc
      421 cacaagcgct tctgtcgctg gcgcgactgc cagtgcgcca actgcctgct ggtggtggag
      481 cggcagcgcg tcatggccgc ccaggtggcg ctccggaggc agcaggccac cgaggacaag
      541 aaggggcttt ccgggaaaca gaataatttc gagcgcaaag ctgtgtacca gaggcaagtc
      601 agagccccca gtttgctggc caaaagcatt ttagaaggct atcgccccat tccagcggag
      661 acttatgtag gagggacctt ccctctacct cccccagtta gtgacaggat gaggaaaaga
      721 agagcctttg ctgataaaga gttggagaac attatgctgg agagagaata taaagaaagg
      781 gagatgttgg aaacttctca agctgctgct ctgtttctgc ccaaccgcat ggtgcctgga
      841 cctgactaca attcctacaa aagtgcctac agccccagcc cagtggaacc accaagcaag
      901 gacttctgta attttttgcc cacctgcctt gatttaacca tgcagtattc agggtctggg
      961 aatatggaac taatttcttc taatgtcagc gtggccacaa cttatagaca gtatcccttg
     1021 tcctcaagat ttttagtttg gcccaagtgt ggccccatta gcgacaccct cctctaccag
     1081 caatgcctgc taaatgccac cacctcagtt caagccctga agcctggggc cagctgggac
     1141 ttgaagggag cacgagtcca ggatggactc agtgcagagc aggacatgat gccatcgaaa
     1201 ttggaaggtt ccctggtgct gcctcacact cctgagatcc agaccacgag aagtgacctt
     1261 cagggtcatc aggctgtccc agagaggtcc gcgttctccc caccccgacg gaatttctct
     1321 cccattgttg acacggactc cctggcagct caagggcatg tcttaacgaa gatcagcaaa
     1381 gaaaacacca ggcaccctct gccacttaga cataatccat tccactcatt attccagcaa
     1441 acacttactg acaaatcggg tcctgagttg aaaacaccat ttgtcaaaga ggcctttgaa
     1501 gagaccccta agaaacacag agagtgttta gttaaggaca accagaagta cacatttaca
     1561 atagatagat gtgcaaaaga cctttttgta gccaaacaag ttggaacaaa actctcggtg
     1621 aatgaaccac tgtcattttc tgttgagtct attcttaaga ggccttcatc tgccatcact
     1681 cgtgtctctc agtagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     1741 tatatatctt gtggaaagga cgaacatctc accacgctgg gctatta
//
