LOCUS       sequence-285042-        9299 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9299)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9299)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1770
FEATURES             Location/Qualifiers
     source          1..9299
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     RBS             5095..5103
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 accgccacca tggaggagaa acagcagatt atattggcta atcaagatgg tggaacagtg
       61 gcaggagcag cacctacctt ctttgtcatc ttaaagcagc caggaaatgg caaaactgat
      121 caaggaattt tggttactaa tcaggatgcc tgtgctttgg ctagtagtgt gtcatcacca
      181 gtaaaatcta aagggaagat ttgccttcca gctgattgta ctgtgggtgg aatcactgtt
      241 accctcgata acaatagtat gtggaatgag ttctatcatc gaagcacaga gatgattctg
      301 accaagcaag gaagacgcat gtttccttac tgtcgttatt ggataacagg tttagattca
      361 aatttgaagt atattcttgt catggatata tctcctgtgg ataaccatcg ttataagtgg
      421 aatggtcgtt ggtgggaacc tagtgggaag gctgaacctc atgttttggg gagggttttc
      481 attcatccag aatctccttc cacaggtcat tattggatgc atcaaccagt atctttctat
      541 aaactcaaac ttaccaacaa tacactggac caagaagggc atatcatctt gcactctatg
      601 catcgttacc tgccgaggct tcatttggtg cctgcagaaa aggctgtgga ggtgatacaa
      661 ttaaatggcc ctggtgtcca cacttttacc ttcccacaga ctgaattctt tgcagtaaca
      721 gcttatcaga acattcagat tactcagctg aaaatagatt acaatccatt tgccaaaggc
      781 tttcgggatg atgggctgaa taataagccc cagagagatg gaaaacaaaa gaacagctct
      841 gaccaagaag ggaataatat ttccagttct tctggtcatc gggtccgtct tacagaaggt
      901 caggggtcag agatacaacc aggtgatttg gatcctttgt caaggggtca tgaaacatca
      961 ggcaagggtt tggagaagac ttcccttaat ataaaacgag actttcttgg tttcatggat
     1021 actgattcag cacttagtga agttcctcaa ttgaagcaag agatttctga atgtcttatt
     1081 gccagcagtt ttgaagatga ctcccgtgta gcctcaccgt tagaccagaa cggaagcttc
     1141 aatgttgtta ttaaagagga acctctagat gattatgact acgaacttgg tgagtgccca
     1201 gaaggggtca ctgtgaaaca ggaagagaca gatgaagaga cggatgtata ctcaaacagt
     1261 gatgatgatc ctatactaga gaaacagcta aagaggcaca ataaagttga caacccagaa
     1321 gctgaccatc tatcttctaa atggcttcca agcagcccat caggtgttgc taaagctaaa
     1381 atgttcaaat tagacactgg aaagatgcca gtagtctatc tggagccctg tgctgtcacc
     1441 agaagcacag ttaagatttc tgaactcccc gataacatgc tttccacatc tcgaaaggat
     1501 aaatcttcta tgttggcaga attggaatat ttgcctacat acattgaaaa ttccaatgag
     1561 actgccttct gcttaggcaa ggaatcagaa aatggtctta gaaaacattc accagatctc
     1621 agagtggtac aaaaatatcc cttactgaaa gagcctcagt ggaaatatcc tgatatatct
     1681 gacagcatta gcacagaaag aatactcgac gattcaaagg attcagttgg agactcactt
     1741 tcaggaaaag aggacttggg cagaaagaga acaactatgc ttaagattgc aacagccgca
     1801 aaggtagtga atgctaatca gaatgcctct ccaaatgtcc ctggaaaaag aggaaggcca
     1861 cgaaaattga aactctgtaa ggcaggacga ccacctaaga acacaggaaa gtctttaatt
     1921 tctacaaaga atacacctgt aagccctggg agtacctttc cagatgtgaa gcctgatctg
     1981 gaagatgtgg atggtgttct ctttgtttcc tttgaatcaa aggaagctct agacattcat
     2041 gcagttgatg ggacaacaga agaatcttct agtctccagg catcaaccac aaatgactca
     2101 ggttacagag caagaatttc ccagttggaa aaggaattga tagaagattt gaagactttg
     2161 cggcacaagc aggtgataca tcctggtctt caagaagtgg gcttaaaatt gaattcagtg
     2221 gatccaacaa tgagcattga tcttaaatac ttgggagtac agttaccttt ggctccagct
     2281 actagctttc ctttttggaa ccttacagga accaaccctg cctctcctga tgcgggattt
     2341 ccctttgttt ctaggacagg gaaaaccaat gatttcacta agatcaaggg atggagggga
     2401 aaatttcata gtgcttctgc atctaggaat gaaggtggaa attcagaaag ttcactgaaa
     2461 aatcgttctg ctttctgtag tgataagcta gatgaatact tggaaaatga aggcaagctg
     2521 atggaaacaa gcatgggttt ttcttctaat gctcccacat ctcctgtggt gtaccagctt
     2581 cccactaaga gtaccagtta tgtacgaaca cttgatagtg tactaaagaa gcaatctact
     2641 atttcccctt ctacctctta ttctttgaaa cctcattctg taccccctgt ctctcgaaag
     2701 gcaaagtctc aaaacagaca ggcaactttc agtggccgaa ctaaatcatc ttataaatcc
     2761 attttaccat accctgtttc accaaagcag aaatactctc atgtgattct aggagataag
     2821 gttaccaaga attcttcagg catcatctca gaaaatcagg cgaataactt tgttgtgcca
     2881 actttggatg aaaatatatt tccaaagcag attagtttgc ggcaggcaca gcagcagcag
     2941 caacagcaac agggaagtcg ccctccaggc ttgtctaaat ctcaggtgaa gctaatggac
     3001 ctggaagact gtgcactttg ggaaggaaaa ccaaggacat acatcacaga agagcgagca
     3061 gatgtatcct taacaactct acttacagct caagcatccc tcaaaactaa acctatccac
     3121 acaatcataa ggaaacgagc ccctccctgc aacaatgact tctgtcgact gggttgtgta
     3181 tgttccagtc tagctttgga gaagcgccaa cctgctcact gccgccgacc agactgcatg
     3241 tttggttgta cttgtttgaa aagaaaagtt gtacttgtta aaggaggatc caaaactaag
     3301 cattttcaga ggaaggctgc tcatcgagat ccagtatttt atgatactct gggagaggag
     3361 gcaagggagg aggaagaagg aatcagggag gaggaggaac aattgaaaga gaaaaagaag
     3421 agaaagaagc tagaatacac tatatgtgag acagagcctg aacagcctgt tcgacattac
     3481 ccattatggg taaaagtaga aggtgaagta gatccagaac cagtttatat ccccacgcct
     3541 tctgtcattg agcctatgaa accattgtta ttgcctcagc cagaagtttt atctcctact
     3601 gtgaagggca aactgctcac tggaattaaa tctccacggt catatactcc caaacccaat
     3661 cctgtgattc gggaagagga caaagatcca gtctacttgt actttgaaag tatgatgact
     3721 tgtgctcgag ttcgagtata tgagcgaaaa aaagaggacc agagacaacc atcttcctcc
     3781 tcctccccat ctccatcatt tcagcagcaa acttcatgtc attctagccc tgagaaccat
     3841 aataatgcaa aggaacctga ttctgaacag cagcccttaa aacaactcac ctgtgacttg
     3901 gaggatgatt ctgataaatt acaagaaaag agctggaagt cttcctgcaa tgaaggagaa
     3961 tcctcttcta cttcttatat gcatcagagg tcacctggtg gtcccaccaa actgattgag
     4021 atcatctcag actgcaactg ggaggaagat cggaacaaga ttttgagcat cttatcccag
     4081 cacatcaata gcaacatgcc acaatcactt aaggtgggca gcttcatcat tgagttggct
     4141 tctcagcgaa agagccgggg tgagaagaac cctcctgttt attcttctcg tgtgaaaatc
     4201 tctatgccat catgtcaaga ccaagatgat atggctgaga aatctggatc agagactcct
     4261 gatggtccat tgtcccctgg gaaaatggag gatatctctc ctgtgcagac agatgccctg
     4321 gattcagtga gggagagatt acatggaggc aaaggtctgc ctttttatgc agggctttct
     4381 cctgcaggga agcttgtggc ctataaacgt aaacccagtt caagtacatc tgggcttatc
     4441 caggtagcat ccaatgccaa ggtggctgca tccaggaaac cacgtaccct gttgccttca
     4501 acatccaatt ccaaaatggc atcctcctct ggcactgcaa caaatcgccc tgggaagaat
     4561 ctgaaggcgt ttgtcccagc aaaacggcca attgcggctc gaccctctcc tggtggtgtg
     4621 ttcacacagt ttgtgatgag taaagttgga gccttgcagc agaagatacc tggagttagc
     4681 acaccccaaa ccctggcagg gacacagaag ttcagtatca gaccttctcc agtaatggtc
     4741 gtcacacctg tggtttcttc tgagccagtt caggtgtgca gccctgtgac tgctgctgtc
     4801 actactacca cccctcaagt gtttttagaa aatactactg ctgtgacacc tatgactgct
     4861 atttctgacg tggaaactaa agaaactact tattcttctg gtgccaccac tacaggggtt
     4921 gttgaggtct ctgaaactaa taccagcacc tctgtaacat ctacccagtc tacagccact
     4981 gtgaacctta ccaaaaccac tgggataact acccctgtgg cttcagttgc ttttcctaag
     5041 tctttggtag catctccttc aaccataact cttcctgttg cttccactgc ttccacctcc
     5101 ttagtcgtgg tgactgcagc tgcatcttcc tccatggtga ccacaccaac ttcatctctg
     5161 ggctctgttc ctattatact ctcaggaatt aatgggagtc caccagtgag ccagagacca
     5221 gaaaatgctg ctcaaattcc agtggctact ccacaggtct ctcctaacac agtgaaacgt
     5281 gctggacctc gattgttgtt gattccagtg cagcagggtt ctcctactct tagacctgtc
     5341 tcaaacacac aacttcaggg acatcggatg gtcttgcagc ctgttaggag tccaagtgga
     5401 atgaacttat tcaggcaccc taatgggcag attgtccagc ttctaccttt gcatcagctt
     5461 cgaggctcta atacccagcc caacttacag cctgtcatgt ttcggaaccc agggtctgtg
     5521 atgggaatcc ggttacctgc tccttccaaa ccctctgaga ctccgccatc ttccacttcg
     5581 tcctctgctt tctctgtcat gaatcctgta attcaagctg ttgggtcttc ttcagcagtg
     5641 aatgttatca ctcaggcacc atcattgctt tcctctggag ctagttttgt gtctcaggct
     5701 ggtacattga ccctgaggat ttctcctcct gaaccacaaa gctttgcaag taaaacaggc
     5761 tctgaaacca aaataactta tagctcagga ggacagcctg ttggtacagc cagtcttatt
     5821 cctctccagt ctggtagttt tgccttgtta cagctcccag gacaaaagcc tgttcctagc
     5881 tccattcttc agcatgttgc ttcccttcag atgaagagag aatctcagaa tccagaccag
     5941 aaagatgaaa caaactcaat aaaaagagag caagaaacga agaaggttct acagtcagaa
     6001 ggagaggctg tagaccctga ggctaatgta ataaaacaaa actcaggagc tgctacctca
     6061 gaagaaactc tgaatgattc cttggaagat aggggtgatc atttggatga agaatgcctt
     6121 ccagaagaag gttgtgcaac tgtcaaacca tctgagcatt cctgtatcac tgggtcacat
     6181 acagatcaag attataaaga tgttaatgaa gaatatgggg ctaggaatcg taagagttcc
     6241 aaagaaaaag tggctgttct ggaagttagg accatttctg aaaaagccag taataagaca
     6301 gtccaaaatt taagtaaagt acagcatcaa aaacttggtg atgtgaaggt ggaacagcag
     6361 aaaggatttg acaatccaga agaaaactca agtgaatttc cagtcacctt taaggaagaa
     6421 agtaaatttg aattgtcagg aagcaaagtt atggagcagc aatctaatct acagccagag
     6481 gccaaagaga aggaatgtgg agactctctg gagaaagaca gggaaagatg gagaaaacat
     6541 ctgaagggcc ccttaaccag gaaatgtgtt ggagcttcac aggaatgtaa gaaagaggca
     6601 gacgagcagt taattaaaga aacaaagaca tgtcaggaaa attcagatgt gtttcagcaa
     6661 gaacaaggca tctctgactt acttggaaaa agtggaatta ctgaagatgc cagagttttg
     6721 aaaactgaat gtgattcttg gagtaggatt tctaatcctt cagccttctc cattgttcct
     6781 aggagagctg caaaaagcag cagagggaat ggacattttc agggtcactt actgctacct
     6841 ggagaacaga tacaaccaaa gcaagagaag aagggtggga gaagcagtgc tgacttcact
     6901 gttttggatt tggaagaaga tgatgaagat gataatgaga aaactgatga ttctattgat
     6961 gaaattgtgg atgttgtttc tgactaccag agtgaggagg ttgatgatgt agaaaagaat
     7021 aactgtgtag aatacattga ggatgatgag gagcacgtgg acattgagac tgtagaagag
     7081 ctctcagagg aaattaatgt tgctcacctg aagaccacag cggcccacac acagtcattc
     7141 aaacagccgt cctgtactca catctctgca gatgaaaaag cagctgaaag gagtcgaaag
     7201 gctccaccaa ttcctctaaa actgaagcct gattactgga gtgacaaact acagaaagaa
     7261 gcagaagcgt ttgcttatta tcgccggaca cacactgcca atgagcggcg gcggcgtggt
     7321 gaaatgaggg atctctttga gaaattaaag atcacattgg gattacttca ttcttccaag
     7381 gtttccaaaa gtctcattct tactcgagcc ttcagtgaaa ttcagggact aacagatcag
     7441 gcagacaaat tgataggaca gaaaaatctc ctgactcgaa aacggaatat tctgatacgg
     7501 aaagtatcgt ctctttcagg taagacagaa gaagtggtcc tgaagaagct agagtatatt
     7561 tatgcaaaac agcaagcact agaggcacaa aaaagaaaaa agaagatggg atcagatgag
     7621 tttgacatat ctcccagaat tagcaaacag caggaaggat cttctgcatc atctgtagat
     7681 cttggacaga tgtttataaa taacaggagg gggaaacctt tgattctttc cagaaaaaaa
     7741 gaccaggcca cagaaaatac ctcacccttg aacactccac acacctctgc caaccttgtg
     7801 atgactccgc aagggcaatt gctcacccta aaaggtcccc tattctcagg accagtggta
     7861 gctgtttctc ctgatctctt agaatctgat cttaagcctc aagttgccgg tagtgctgtg
     7921 gctctaccag aaaatgacga cttatttatg atgccacgaa ttgttaatgt gacatcattg
     7981 gccacagagg gaggtttggt agatatgggt ggcagcaaat atcctcatga agttcctgat
     8041 agcaagccat ctgaccatct gaaagacacc gtcaggaatg aagataattc cttagaggat
     8101 aagggtagaa tctcttccag aggaaacaga gatggcagag tgacgttggg tccaacgcag
     8161 gtttttctgg caaacaaaga ttctggttat ccacaaatag ttgacgtttc caatatgcag
     8221 aaagcacaag agttcttacc taaaaagatt tctggtgata tgagagggat tcagtataaa
     8281 tggaaagaga gtgaatcaag aggggagaga gtgaagtcaa aggattcttc atttcataaa
     8341 ttaaagatga aagatctcaa ggactcaagc atagagatgg aactgaggaa agtaacatca
     8401 gctatagagg aagcagctct tgattccagt gaactgctga ctaacatgga agatgaggat
     8461 gatactgatg agacactgac ttcactgctc aatgaaattg cctttcttaa tcaacaacta
     8521 aatgatgact ctgttggcct ggctgaacta cccagctcta tggatacaga gttcccaggg
     8581 gatgctcggc gggcttttat tagtaaggtt cctcctggaa gcagagcaac tttccaggtt
     8641 gagcacttgg gaactggttt gaaagagttg cctgatgttc aaggggagag tgactctatc
     8701 agtcccctcc tcttgcactt ggaagacgat gacttttctg agaatgaaaa acaacttgca
     8761 gaaccagcct ctgagccaga tgtccttaag attgttattg actctgaaat aaaggattcc
     8821 ctcctttcca acaagaaagc tattgatgga gggaagaata cttctggcct ccctgcagag
     8881 cccgaaagtg tgtcctcacc ccccacccta cacatgaaga ctggcttgga gaacagcaac
     8941 agcacagaca ctttgtggag gcctatgcca aagttggccc ctctaggttt aaaagtagct
     9001 aatccttcca gtgatgcaga tggtcagagt ctcaaggtga tgccttgttt ggcacctata
     9061 gctgccaaag ttgggtcagt tggacacaaa atgaacttaa cagggaatga ccaggaaggc
     9121 cgggaaagca aggtgatgcc tacattggca cctgttgtgg ctaaattggg caactcgggg
     9181 gcctcaccaa gttctgcagg gaaatagtaa tgaactagtc cgtaacttga aagtatttcg
     9241 atttcttggc tttatatatc ttgtggaaag gacgatgttg cgtatggagt taagagtac
//
