LOCUS       sequence-285051-        2441 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1813
FEATURES             Location/Qualifiers
     source          1..2441
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1882..1893
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 accgccacca tgctgccatg tcattcacca tcacgctctg atcaggtgaa tctgggaccc
       61 tccatcaact ccaagctgct ggggatgtcc acccagaact atgccctgat gcaggttgct
      121 ggccaggagg ggacattttc tcttgttgct ctgccacatg ttgcctcagc tcagccaatt
      181 cagaaaccca gaatgtccct acctgaaaac ctgaaacttc ctattcctag atatcaaccc
      241 cctagaaata gcaaagcatc aagaaagaaa cccatcctga tctttcctaa gagtggctgt
      301 agcaaagctc ctgcccaaac ccaaatgtgt cctcagatgt ccccttcccc acctcaccac
      361 cctgaactcc tgtacaaacc cagtccattt gaggaagtac catcactaga gcaagcccca
      421 gccagcatta gcacagctgc gctgaccaat ggaagtgacc atggggactt gagaccacca
      481 gtgaccaaca cccatggcag tctgaaccct cctgctaccc cagcatcatc cacaccagag
      541 gagcctgcca agcaggacct cacagctctt tcagggaaag cacactttgt aagcaagata
      601 acatctagta aaccttctgc tgttgccagt gaaaaattta aagaacaagt tgatcttgca
      661 aaaaccatga ccaatttatc accaaccatt cttggcaatg cagttcagtt gatctcttca
      721 gtccccaaag ggaaactgcc aatcccaccc tactcaagaa tgaagacaat ggaggtttac
      781 aaaatcaaat cagatgctaa cattgcaggt ttttctttac caggacctaa ggccgactgt
      841 gataagatac cctccaccac agaaggcttt aatgcagcca ccaaggtggc aagcaggcta
      901 cctgttccac aagtgtcaca gcagagtgcc tgtgaaagtg ccttttgtcc acccaccaaa
      961 cttgatctta accacaaaac aaaactgaac agtggagcag caaagagaaa aggaagaaaa
     1021 cggaaggtac cagatgaaat tttggcattt cagggaaaaa ggaggaaata tatcattaat
     1081 aagtgtagag atggtaaaga aagagtaaaa aatgatcccc aagaattcag agaccaaaag
     1141 ctggggaccc tgaaaaaata ccgtagcatt atgcccaaac ctatcatggt catacccact
     1201 ttggcctccc tggcttctcc aactacacta cagtcccaga tgcttggggg cctaggacag
     1261 gatgttttgt taaataattc actcactcct aaatatcttg gctgtaagca agacaacagc
     1321 tcttccccta agcccagctc cgtgttcaga aatggattct ctggcattaa gaagccttgg
     1381 cacagatgtc acgtctgcaa ccaccacttc cagttcaaac agcaccttcg agaccacatg
     1441 aatacacaca ccaacagacg cccttacagt tgtcggattt gtcgcaagtc ctatgtacgt
     1501 cctggcagcc tgagcacaca catgaaactt catcatggtg agaaccgtct gaagaaactc
     1561 atgtgttgtg agttttgtgc aaaagtgttt ggccacatcc gagtctattt tggccatctg
     1621 aaagaagtgc atagggttgt gatcagcact gagcctgcgc ccagtgaact gcagccagga
     1681 gacataccaa agaacagaga catgagtgtg cgaggcatgg agggatcatt ggagagggaa
     1741 aacaagtcca acctggaaga agacttcctt ctaaaccagg cagacgaagt caaattacaa
     1801 atcaaatgtg gtcgttgtca gattactgct cagtcttttg cggaaataaa atttcattta
     1861 cttgatgttc atggagagga aattgagggc aggctacaag aagggacctt cccaggaagc
     1921 aaggggactc aggaagagtt ggtgcagcac gctagccccg actggaaaag gcatcctgag
     1981 agagggaagc cggagaaggt tcattcctcc tccgaggaat cacatgcatg tccaagactg
     2041 aaaaggcagc tccaccttca tcagaatggc gtggaaatgc tcatggaaaa tgaaggaccc
     2101 cagtcaggaa ccaacaagcc aagggaaacc tgccagggcc ctgagtgtcc tggcctccac
     2161 acgtttctct tgtggtccca ttcaggcttt aactgcctgc tttgtgcaga gatgctggga
     2221 cggaaagagg acctcctcca ccactggaag caccagcata actgtgagga cccttccaaa
     2281 ctgtgggcta ttttaaatac ggtctccaac cagggagtga tcgaactttc cagtgaagct
     2341 gagaaatagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     2401 tcttgtggaa aggacgatct cctgaacagt cctatcccac c
//
