LOCUS       sequence-285057-        5552 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5552)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 5552)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1888
FEATURES             Location/Qualifiers
     source          1..5552
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 accgccacca tgctgaaact tctcaaccag aagaaagggc cttcacagtg tcctttatgt
       61 aagaatgata taaccaaaag gagcctacaa gaaagtacga gatttagtca acttgttgaa
      121 gagctattga aaatcatttg tgcttttcag cttgacacag gtttggagta tgcaaacagc
      181 tataattttg caaaaaagga aaataactct cctgaacatc taaaagatga agtttctatc
      241 atccaaagta tgggctacag aaaccgtgcc aaaagacttc tacagagtga acccgaaaat
      301 ccttccttgc aggaaaccag tctcagtgtc caactctcta accttggaac tgtgagaact
      361 ctgaggacaa agcagcggat acaacctcaa aagacgtctg tctacattga attgggatct
      421 gattcttctg aagataccgt taataaggca acttattgca gtgtgggaga tcaagaattg
      481 ttacaaatca cccctcaagg aaccagggat gaaatcagtt tggattctgc aaaaaaggct
      541 gcttgtgaat tttctgagac ggatgtaaca aatactgaac atcatcaacc cagtaataat
      601 gatttgaaca ccactgagaa gcgtgcagct gagaggcatc cagaaaagta tcagggtagt
      661 tctgtttcaa acttgcatgt ggagccatgt ggcacaaata ctcatgccag ctcattacag
      721 catgagaaca gcagtttatt actcactaaa gacagaatga atgtagaaaa ggctgaattc
      781 tgtaataaaa gcaaacagcc tggcttagca aggagccaac ataacagatg ggctggaagt
      841 aaggaaacat gtaatgatag gcggactccc agcacagaaa aaaaggtaga tctgaatgct
      901 gatcccctgt gtgagagaaa agaatggaat aagcagaaac tgccatgctc agagaatcct
      961 agagatactg aagatgttcc ttggataaca ctaaatagca gcattcagaa agttaatgag
     1021 tggttttcca gaagtgatga actgttaggt tctgatgact cacatgatgg ggagtctgaa
     1081 tcaaatgcca aagtagctga tgtattggac gttctaaatg aggtagatga atattctggt
     1141 tcttcagaga aaatagactt actggccagt gatcctcatg aggctttaat atgtaaaagt
     1201 gaaagagttc actccaaatc agtagagagt aatattgaag acaaaatatt tgggaaaacc
     1261 tatcggaaga aggcaagcct ccccaactta agccatgtaa ctgaaaatct aattatagga
     1321 gcatttgtta ctgagccaca gataatacaa gagcgtcccc tcacaaataa attaaagcgt
     1381 aaaaggagac ctacatcagg ccttcatcct gaggatttta tcaagaaagc agatttggca
     1441 gttcaaaaga ctcctgaaat gataaatcag ggaactaacc aaacggagca gaatggtcaa
     1501 gtgatgaata ttactaatag tggtcatgag aataaaacaa aaggtgattc tattcagaat
     1561 gagaaaaatc ctaacccaat agaatcactc gaaaaagaat ctgctttcaa aacgaaagct
     1621 gaacctataa gcagcagtat aagcaatatg gaactcgaat taaatatcca caattcaaaa
     1681 gcacctaaaa agaataggct gaggaggaag tcttctacca ggcatattca tgcgcttgaa
     1741 ctagtagtca gtagaaatct aagcccacct aattgtactg aattgcaaat tgatagttgt
     1801 tctagcagtg aagagataaa gaaaaaaaag tacaaccaaa tgccagtcag gcacagcaga
     1861 aacctacaac tcatggaagg taaagaacct gcaactggag ccaagaagag taacaagcca
     1921 aatgaacaga caagtaaaag acatgacagc gatactttcc cagagctgaa gttaacaaat
     1981 gcacctggtt cttttactaa gtgttcaaat accagtgaac ttaaagaatt tgtcaatcct
     2041 agccttccaa gagaagaaaa agaagagaaa ctagaaacag ttaaagtgtc taataatgct
     2101 gaagacccca aagatctcat gttaagtgga gaaagggttt tgcaaactga aagatctgta
     2161 gagagtagca gtatttcatt ggtacctggt actgattatg gcactcagga aagtatctcg
     2221 ttactggaag ttagcactct agggaaggca aaaacagaac caaataaatg tgtgagtcag
     2281 tgtgcagcat ttgaaaaccc caagggacta attcatggtt gttccaaaga taatagaaat
     2341 gacacagaag gctttaagta tccattggga catgaagtta accacagtcg ggaaacaagc
     2401 atagaaatgg aagaaagtga acttgatgct cagtatttgc agaatacatt caaggtttca
     2461 aagcgccagt catttgctcc gttttcaaat ccaggaaatg cagaagagga atgtgcaaca
     2521 ttctctgccc actctgggtc cttaaagaaa caaagtccaa aagtcacttt tgaatgtgaa
     2581 caaaaggaag aaaatcaagg aaagaatgag tctaatatca agcctgtaca gacagttaat
     2641 atcactgcag gctttcctgt ggttggtcag aaagataagc cagttgataa tgccaaatgt
     2701 agtatcaaag gaggctctag gttttgtcta tcatctcagt tcagaggcaa cgaaactgga
     2761 ctcattactc caaataaaca tggactttta caaaacccat atcgtatacc accacttttt
     2821 cccatcaagt catttgttaa aactaaatgt aagaaaaatc tgctagagga aaactttgag
     2881 gaacattcaa tgtcacctga aagagaaatg ggaaatgaga acattccaag tacagtgagc
     2941 acaattagcc gtaataacat tagagaaaat gtttttaaag aagccagctc aagcaatatt
     3001 aatgaagtag gttccagtac taatgaagtg ggctccagta ttaatgaaat aggttccagt
     3061 gatgaaaaca ttcaagcaga actaggtaga aacagagggc caaaattgaa tgctatgctt
     3121 agattagggg ttttgcaacc tgaggtctat aaacaaagtc ttcctggaag taattgtaag
     3181 catcctgaaa taaaaaagca agaatatgaa gaagtagttc agactgttaa tacagatttc
     3241 tctccatatc tgatttcaga taacttagaa cagcctatgg gaagtagtca tgcatctcag
     3301 gtttgttctg agacacctga tgacctgtta gatgatggtg aaataaagga agatactagt
     3361 tttgctgaaa atgacattaa ggaaagttct gctgttttta gcaaaagcgt ccagaaagga
     3421 gagcttagca ggagtcctag ccctttcacc catacacatt tggctcaggg ttaccgaaga
     3481 ggggccaaga aattagagtc ctcagaagag aacttatcta gtgaggatga agagcttccc
     3541 tgcttccaac acttgttatt tggtaaagta aacaatatac cttctcagtc tactaggcat
     3601 agcaccgttg ctaccgagtg tctgtctaag aacacagagg agaatttatt atcattgaag
     3661 aatagcttaa atgactgcag taaccaggta atattggcaa aggcatctca ggaacatcac
     3721 cttagtgagg aaacaaaatg ttctgctagc ttgttttctt cacagtgcag tgaattggaa
     3781 gacttgactg caaatacaaa cacccaggat cctttcttga ttggttcttc caaacaaatg
     3841 aggcatcagt ctgaaagcca gggagttggt ctgagtgaca aggaattggt ttcagatgat
     3901 gaagaaagag gaacgggctt ggaagaaaat aatcaagaag agcaaagcat ggattcaaac
     3961 ttaggtgaag cagcatctgg gtgtgagagt gaaacaagcg tctctgaaga ctgctcaggg
     4021 ctatcctctc agagtgacat tttaaccact cagcagaggg ataccatgca acataacctg
     4081 ataaagctcc agcaggaaat ggctgaacta gaagctgtgt tagaacagca tgggagccag
     4141 ccttctaaca gctacccttc catcataagt gactcttctg cccttgagga cctgcgaaat
     4201 ccagaacaaa gcacatcaga aaaagcagta ttaacttcac agaaaagtag tgaataccct
     4261 ataagccaga atccagaagg cctttctgct gacaagtttg aggtgtctgc agatagttct
     4321 accagtaaaa ataaagaacc aggagtggaa aggtcatccc cttctaaatg cccatcatta
     4381 gatgataggt ggtacatgca cagttgctct gggagtcttc agaatagaaa ctacccatct
     4441 caagaggagc tcattaaggt tgttgatgtg gaggagcaac agctggaaga gtctgggcca
     4501 cacgatttga cggaaacatc ttacttgcca aggcaagatc tagagggaac cccttacctg
     4561 gaatctggaa tcagcctctt ctctgatgac cctgaatctg atccttctga agacagagcc
     4621 ccagagtcag ctcgtgttgg caacatacca tcttcaacct ctgcattgaa agttccccaa
     4681 ttgaaagttg cagaatctgc ccagagtcca gctgctgctc atactactga tactgctggg
     4741 tataatgcaa tggaagaaag tgtgagcagg gagaagccag aattgacagc ttcaacagaa
     4801 agggtcaaca aaagaatgtc catggtggtg tctggcctga ccccagaaga atttatgctc
     4861 gtgtacaagt ttgccagaaa acaccacatc actttaacta atctaattac tgaagagact
     4921 actcatgttg ttatgaaaac agatgctgag tttgtgtgtg aacggacact gaaatatttt
     4981 ctaggaattg cgggaggaaa atgggtagtt agctatttct gggtgaccca gtctattaaa
     5041 gaaagaaaaa tgctgaatga gcatgatttt gaagtcagag gagatgtggt caatggaaga
     5101 aaccaccaag gtccaaagcg agcaagagaa tcccaggaca gaaagatctt cagggggcta
     5161 gaaatctgtt gctatgggcc cttcaccaac atgcccacag atcaactgga atggatggta
     5221 cagctgtgtg gtgcttctgt ggtgaaggag ctttcatcat tcacccttgg cacaggtgtc
     5281 cacccaattg tggttgtgca gccagatgcc tggacagagg acaatggctt ccatgcaatt
     5341 gggcagatgt gtgaggcacc tgtggtgacc cgagagtggg tgttggacag tgtagcactc
     5401 taccagtgcc aggagctgga cacctacctg ataccccaga tcccccacag ccactactag
     5461 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     5521 aaggacgaac tgaactgatc ttggcgttat ac
//
