LOCUS       sequence-285069-        1220 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1220)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1971
FEATURES             Location/Qualifiers
     source          1..1220
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             392..409
                     /codon_start=1
                     /product="HQHQHQ metal affinity tag"
                     /label=HQ tag
                     /note="The HQ tag binds to nickel and other metal ions much
                     like the 6xHis tag, but may allow elution at lower 
                     concentrations of imidazole (Godat et al., 2008)."
                     /translation="HQHQHQ"
ORIGIN
        1 accgccacca tggcctccaa cgaagatttc tccatcacac aagacctgga gatcccggca
       61 gatattgtgg agctccacga catcaatgtg gagccccttc ctatggagga cattccgacg
      121 gaaagcgtcc agtacgagga tgtggatggc aattggatct acggtggcca caaccatccg
      181 ccattgatgg tgttgcagcc gctcttcacg aacacgggct atggcgacca cgaccaggaa
      241 atgcttatgt tgcagacaca agaggaagtg gtgggctatt gcgactcaga caaccagcta
      301 ggcaacgact tggaggacca gttggccctc ccggatagca ttgaagacga gcacttccag
      361 atgaccctgg cctctctgtc ggcctcggcg gcatcaacat caacatcaac ccagagccgc
      421 agcaaaaagc ccagcaaaaa gcccagcggc aagagtgcca ccagcactga ggccaacccg
      481 gcaggcagca gctccagcct gggcacgagg aagtgggagc agaagcaaat gcaggtcaaa
      541 acgctggagg gtgagttttc cgtgactatg tggtccccta acgataacaa tgaccaaggg
      601 gcagtgggtg aaggccaggc tgaaaaccca cctgattatt ccgagtactt gaaagggaag
      661 aaacttcctc ctggggggtt accaggcatt gatctctcag atcctaaaca gctggcagaa
      721 tttactaaag tgaagcccaa aaggtccaaa ggagaacctc ccaaaacagt cccttgctct
      781 tatagcggct gcgaaaagat gttccgggat tacgccgcca tgagaaaaca tctccacatc
      841 cacgggccca gagtccacgt atgtgcagaa tgtggcaaag cttttcttga gagctcaaag
      901 ctgagacgac accagctggt ccacaccggc gagaagccct ttcagtgcac attcgaaggc
      961 tgcgggaaac gcttttccct tgatttcaat ttgcgcacac acttgcgcat ccacaccggc
     1021 gataagccct tcgtgtgccc cttcgatgtt tgcaacagga agttcgctca gtcaaccaac
     1081 ctgaaaaccc acatattaac gcatgtgaag accaaaaaca acccgtagta atgaactagt
     1141 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaacct
     1201 gggcctctgg aactacactc
//
