LOCUS       sequence-285084-        2864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2023
FEATURES             Location/Qualifiers
     source          1..2864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcgcccac caagcccagc tttcagcagg atccttccag gcgagaacgt
       61 ttacaagcat tgagaaagga gaaatcccga gatgctgctc gctcccgccg gggaaaagaa
      121 aactttgagt tctatgaatt ggccaagttg ttgcctcttc ctgcagccat taccagccag
      181 ctcgacaagg catccatcat tcgacttaca attagctatc tgaaaatgag ggactttgct
      241 aaccaggggg accctccgtg gaacttgcga atggaaggcc ctccacctaa cacatcagta
      301 aaagggatac aaatgtggaa atctgaactc tgcatgagaa agacaccatg tgaagttata
      361 ggtgcacagc gaaggagaag ccccagtgca ctagccattg aagtatttga agcacatttg
      421 ggaagccaca ttttgcagtc cctggatggc tttgtatttg cactaaatca ggaaggaaaa
      481 tttttgtaca tttccgaaac agtctccatc tacctaggcc tctcacaagt ggagctgaca
      541 ggcagcagtg tctttgacta tgtccacccc ggagatcacg tggagatggc tgagcagctg
      601 ggcatgaagc tcccccctgg gcggggtctc ctgtcacagg gcactgctga ggacggagcc
      661 agctcagcat cttcctcctc tcagtcggag acccccgagc cagtggagtc aaccagcccc
      721 agtctgctaa ccactgacaa cactcttgag cgttcctttt tcatccgaat gaaatctact
      781 ctgaccaaac gcggtgtgca catcaaatca tcaggatata aggtgattca cataacaggc
      841 cggctacgcc tgagagtgtc gctgtcccac gggaggaccg tccccagcca aatcatgggt
      901 ctcgtggttg ttgcgcatgc cttgcctccc cctacgatca atgaagtcag aattgactgc
      961 catatgttcg tcactcgagt aaatatggac ctcaatatca tttactgtga aaataggatt
     1021 agtgattata tggatctgac ccctgtagat atcgtaggga agagatgcta ccacttcatc
     1081 catgctgaag acgtggaggg catcaggcac agtcacttgg acttgctgaa taagggtcag
     1141 tgtgtgacaa agtactatcg ctggatgcag aagaacggag gatatatttg gatacagtcc
     1201 agtgccacca tagctattaa tgccaagaat gcaaatgaaa agaatatcat ctgggtgaat
     1261 taccttctta gcaatcctga gtacaaggac acacccatgg acatcgcaca gctcccccat
     1321 ctgccggaga aaacttccga atcctcggag acatccgact ctgagtcaga ctctaaagac
     1381 acctcaggta ttacagagga caacgagaac tccaagtccg acgagaaggg gaaccagtcc
     1441 gagaacagcg aagacccgga gcccgaccgg aagaagtcgg gcaacgcgtg tgacaacgac
     1501 atgaactgca acgacgacgg ccacagctcc agtaacccgg acagccgcga cagcgacgac
     1561 agcttcgagc actcggactt tgagaacccc aaggcgggcg aggacggctt cggtgctctg
     1621 ggcgcgatgc agatcaaggt ggagcgctac gtggagagcg agtcggacct gcggctgcag
     1681 aactgcgagt cactcacgtc cgacagcgcc aaggactcgg acagcgcagg cgaggcgggc
     1741 gcgcaggcct ccagcaagca ccagaagcgc aagaaaaggc ggaaacggca aaagggcggc
     1801 agcgccagcc gccggcgcct gtccagcgcg tcgagcccag gcggcctgga cgcgggcctg
     1861 gtggagcccc cgcggctgct gtcctccccc aacagtgcct cggtgctcaa gatcaagacg
     1921 gagatctcag aacccatcaa tttcgacaat gacagcagca tctggaacta cccgcccaac
     1981 cgggagatct ccaggaacga gtccccctac agcatgacca agccccccag ctctgagcac
     2041 ttcccgtccc cgcagggcgg cggcggtggg ggtggcggtg gcggggggct gcacgtggcc
     2101 attcccgact cggtcctcac cccgcccggc gccgacggcg cggccgcccg caagactcag
     2161 ttcggcgcct cggccaccgc ggccctggcc cccgtcgcct ccgacccgct gtcacccccg
     2221 ctctcggcgt ccccgcggga caagcacccc gggaacggcg gcgggggcgg gggcgggggc
     2281 ggcggcgcgg ggggcggcgg ccccagcgcg tccaactcct tgctgtacac tggggacctg
     2341 gaggcgctgc agaggttgca ggcgggcaac gtcgtgctcc cgctggtgca cagggtgacc
     2401 gggaccctgg ccgccaccag cacggccgcg cagagggtct acaccacggg caccatccgc
     2461 tacgcgcccg ccgaggtgac cctggccatg cagagcaacc tgctgcccaa cgcgcacgct
     2521 gttaacttcg tggacgttaa cagccccggc tttggcctcg accccaagac gcccatggag
     2581 atgctctacc accacgtgca ccggctcaac atgtcaggac cgttcggcgg cgcagtgagc
     2641 gcagctagcc tgacgcagat gcccgccggc aacgtgttca ccacggccga gggactcttc
     2701 tccacgctgc ccttccccgt ctacagcaac ggcatccacg cggcacagac tctggagcgc
     2761 aaggaggact agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     2821 atatcttgtg gaaaggacga ctatggattt gggctgtcgg agcg
//
