LOCUS       sequence-285087-        2807 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2024
FEATURES             Location/Qualifiers
     source          1..2807
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcgcccac caagcccagc tttcagcagg atccttccag gcgagaacgt
       61 ttacaagcat tgagaaagga gaaatcccga gatgctgctc gctcccgccg gggaaaagaa
      121 aactttgagt tctatgaatt ggccaagttg ttgcctcttc ctgcagccat taccagccag
      181 ctcgacaagg catccatcat tcgacttaca attagctatc tgaaaatgag ggactttgct
      241 aaccaggggg accctccgtg gaacttgcga atggaaggcc ctccacctaa cacatcagta
      301 aaaggtgcac agcgaaggag aagccccagt gcactagcca ttgaagtatt tgaagcacat
      361 ttgggaagcc acattttgca gtccctggat ggctttgtat ttgcactaaa tcaggaagga
      421 aaatttttgt acatttccga aacagtctcc atctacctag gcctctcaca agtggagctg
      481 acaggcagca gtgtctttga ctatgtccac cccggagatc acgtggagat ggctgagcag
      541 ctgggcatga agctcccccc tgggcggggt ctcctgtcac agggcactgc tgaggacgga
      601 gccagctcag catcttcctc ctctcagtcg gagacccccg agccagtgga gtcaaccagc
      661 cccagtctgc taaccactga caacactctt gagcgttcct ttttcatccg aatgaaatct
      721 actctgacca aacgcggtgt gcacatcaaa tcatcaggat ataaggtgat tcacataaca
      781 ggccggctac gcctgagagt gtcgctgtcc cacgggagga ccgtccccag ccaaatcatg
      841 ggtctcgtgg ttgttgcgca tgccttgcct ccccctacga tcaatgaagt cagaattgac
      901 tgccatatgt tcgtcactcg agtaaatatg gacctcaata tcatttactg tgaaaatagg
      961 attagtgatt atatggatct gacccctgta gatatcgtag ggaagagatg ctaccacttc
     1021 atccatgctg aagacgtgga gggcatcagg cacagtcact tggacttgct gaataagggt
     1081 cagtgtgtga caaagtacta tcgctggatg cagaagaacg gaggatatat ttggatacag
     1141 tccagtgcca ccatagctat taatgccaag aatgcaaatg aaaagaatat catctgggtg
     1201 aattaccttc ttagcaatcc tgagtacaag gacacaccca tggacatcgc acagctcccc
     1261 catctgccgg agaaaacttc cgaatcctcg gagacatccg actctgagtc agactctaaa
     1321 gacacctcag gtattacaga ggacaacgag aactccaagt ccgacgagaa ggggaaccag
     1381 tccgagaaca gcgaagaccc ggagcccgac cggaagaagt cgggcaacgc gtgtgacaac
     1441 gacatgaact gcaacgacga cggccacagc tccagtaacc cggacagccg cgacagcgac
     1501 gacagcttcg agcactcgga ctttgagaac cccaaggcgg gcgaggacgg cttcggtgct
     1561 ctgggcgcga tgcagatcaa ggtggagcgc tacgtggaga gcgagtcgga cctgcggctg
     1621 cagaactgcg agtcactcac gtccgacagc gccaaggact cggacagcgc aggcgaggcg
     1681 ggcgcgcagg cctccagcaa gcaccagaag cgcaagaaaa ggcggaaacg gcaaaagggc
     1741 ggcagcgcca gccgccggcg cctgtccagc gcgtcgagcc caggcggcct ggacgcgggc
     1801 ctggtggagc ccccgcggct gctgtcctcc cccaacagtg cctcggtgct caagatcaag
     1861 acggagatct cagaacccat caatttcgac aatgacagca gcatctggaa ctacccgccc
     1921 aaccgggaga tctccaggaa cgagtccccc tacagcatga ccaagccccc cagctctgag
     1981 cacttcccgt ccccgcaggg cggcggcggt gggggtggcg gtggcggggg gctgcacgtg
     2041 gccattcccg actcggtcct caccccgccc ggcgccgacg gcgcggccgc ccgcaagact
     2101 cagttcggcg cctcggccac cgcggccctg gcccccgtcg cctccgaccc gctgtcaccc
     2161 ccgctctcgg cgtccccgcg ggacaagcac cccgggaacg gcggcggggg cgggggcggg
     2221 ggcggcggcg cggggggcgg cggccccagc gcgtccaact ccttgctgta cactggggac
     2281 ctggaggcgc tgcagaggtt gcaggcgggc aacgtcgtgc tcccgctggt gcacagggtg
     2341 accgggaccc tggccgccac cagcacggcc gcgcagaggg tctacaccac gggcaccatc
     2401 cgctacgcgc ccgccgaggt gaccctggcc atgcagagca acctgctgcc caacgcgcac
     2461 gctgttaact tcgtggacgt taacagcccc ggctttggcc tcgaccccaa gacgcccatg
     2521 gagatgctct accaccacgt gcaccggctc aacatgtcag gaccgttcgg cggcgcagtg
     2581 agcgcagcta gcctgacgca gatgcccgcc ggcaacgtgt tcaccacggc cgagggactc
     2641 ttctccacgc tgcccttccc cgtctacagc aacggcatcc acgcggcaca gactctggag
     2701 cgcaaggagg actagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     2761 tatatatctt gtggaaagga cgatatgagg aatgctgtga gcatgag
//
