LOCUS       sequence-285090-        2813 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2025
FEATURES             Location/Qualifiers
     source          1..2813
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcgcccac caagcccagc tttcagcagg atccttccag gcgagaacgt
       61 ttacaagcat tgagaaagga gaaatcccga gatgctgctc gctcccgccg gggaaaagaa
      121 aactttgagt tctatgaatt ggccaagttg ttgcctcttc ctgcagccat taccagccag
      181 ctcgacaagg catccatcat tcgacttaca attagctatc tgaaaatgag ggactttgct
      241 aaccaggggg accctccgtg gaacttgcga atggaaggcc ctccacctaa cacatcagta
      301 aaagttatag gtgcacagcg aaggagaagc cccagtgcac tagccattga agtatttgaa
      361 gcacatttgg gaagccacat tttgcagtcc ctggatggct ttgtatttgc actaaatcag
      421 gaaggaaaat ttttgtacat ttccgaaaca gtctccatct acctaggcct ctcacaagtg
      481 gagctgacag gcagcagtgt ctttgactat gtccaccccg gagatcacgt ggagatggct
      541 gagcagctgg gcatgaagct cccccctggg cggggtctcc tgtcacaggg cactgctgag
      601 gacggagcca gctcagcatc ttcctcctct cagtcggaga cccccgagcc agtggagtca
      661 accagcccca gtctgctaac cactgacaac actcttgagc gttccttttt catccgaatg
      721 aaatctactc tgaccaaacg cggtgtgcac atcaaatcat caggatataa ggtgattcac
      781 ataacaggcc ggctacgcct gagagtgtcg ctgtcccacg ggaggaccgt ccccagccaa
      841 atcatgggtc tcgtggttgt tgcgcatgcc ttgcctcccc ctacgatcaa tgaagtcaga
      901 attgactgcc atatgttcgt cactcgagta aatatggacc tcaatatcat ttactgtgaa
      961 aataggatta gtgattatat ggatctgacc cctgtagata tcgtagggaa gagatgctac
     1021 cacttcatcc atgctgaaga cgtggagggc atcaggcaca gtcacttgga cttgctgaat
     1081 aagggtcagt gtgtgacaaa gtactatcgc tggatgcaga agaacggagg atatatttgg
     1141 atacagtcca gtgccaccat agctattaat gccaagaatg caaatgaaaa gaatatcatc
     1201 tgggtgaatt accttcttag caatcctgag tacaaggaca cacccatgga catcgcacag
     1261 ctcccccatc tgccggagaa aacttccgaa tcctcggaga catccgactc tgagtcagac
     1321 tctaaagaca cctcaggtat tacagaggac aacgagaact ccaagtccga cgagaagggg
     1381 aaccagtccg agaacagcga agacccggag cccgaccgga agaagtcggg caacgcgtgt
     1441 gacaacgaca tgaactgcaa cgacgacggc cacagctcca gtaacccgga cagccgcgac
     1501 agcgacgaca gcttcgagca ctcggacttt gagaacccca aggcgggcga ggacggcttc
     1561 ggtgctctgg gcgcgatgca gatcaaggtg gagcgctacg tggagagcga gtcggacctg
     1621 cggctgcaga actgcgagtc actcacgtcc gacagcgcca aggactcgga cagcgcaggc
     1681 gaggcgggcg cgcaggcctc cagcaagcac cagaagcgca agaaaaggcg gaaacggcaa
     1741 aagggcggca gcgccagccg ccggcgcctg tccagcgcgt cgagcccagg cggcctggac
     1801 gcgggcctgg tggagccccc gcggctgctg tcctccccca acagtgcctc ggtgctcaag
     1861 atcaagacgg agatctcaga acccatcaat ttcgacaatg acagcagcat ctggaactac
     1921 ccgcccaacc gggagatctc caggaacgag tccccctaca gcatgaccaa gccccccagc
     1981 tctgagcact tcccgtcccc gcagggcggc ggcggtgggg gtggcggtgg cggggggctg
     2041 cacgtggcca ttcccgactc ggtcctcacc ccgcccggcg ccgacggcgc ggccgcccgc
     2101 aagactcagt tcggcgcctc ggccaccgcg gccctggccc ccgtcgcctc cgacccgctg
     2161 tcacccccgc tctcggcgtc cccgcgggac aagcaccccg ggaacggcgg cgggggcggg
     2221 ggcgggggcg gcggcgcggg gggcggcggc cccagcgcgt ccaactcctt gctgtacact
     2281 ggggacctgg aggcgctgca gaggttgcag gcgggcaacg tcgtgctccc gctggtgcac
     2341 agggtgaccg ggaccctggc cgccaccagc acggccgcgc agagggtcta caccacgggc
     2401 accatccgct acgcgcccgc cgaggtgacc ctggccatgc agagcaacct gctgcccaac
     2461 gcgcacgctg ttaacttcgt ggacgttaac agccccggct ttggcctcga ccccaagacg
     2521 cccatggaga tgctctacca ccacgtgcac cggctcaaca tgtcaggacc gttcggcggc
     2581 gcagtgagcg cagctagcct gacgcagatg cccgccggca acgtgttcac cacggccgag
     2641 ggactcttct ccacgctgcc cttccccgtc tacagcaacg gcatccacgc ggcacagact
     2701 ctggagcgca aggaggacta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     2761 tggctttata tatcttgtgg aaaggacgag ttgtcgctaa ccaactgcaa ggc
//
