LOCUS       sequence-285096-        6020 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6020)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 6020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2038
FEATURES             Location/Qualifiers
     source          1..6020
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaccctac tggaagtcag ttggattcag atttctctca gcaagatact
       61 ccttgcctga taattgaaga ttctcagcct gaaagccagg ttctagagga tgattctggt
      121 tctcacttca gtatgctatc tcgacacctt cctaatctcc agacgcacaa agaaaatcct
      181 gtgttggatg ttgtgtccaa tcctgaacaa acagctggag aagaacgagg agacggtaat
      241 agtgggttca atgaacattt gaaagaaaac aaggttgcag accctgtgga ttcttctaac
      301 ttggacacat gtggttccat cagtcaggtc attgagcagt tacctcagcc aaacaggaca
      361 agcagtgttc tgggaatgtc agtggaatct gctcctgctg tggaggaaga gaagggagaa
      421 gagttggaac agaaggagaa agagaaggaa gaagatactt caggcaatac tacacattcc
      481 cttggtgctg aagatactgc ctcatcacag ttgggttttg gggttctgga actctcccag
      541 agccaggatg ttgaggaaaa tactgtgcca tatgaagtgg acaaagagca gctacaatca
      601 gtaaccacca actctggtta taccaggctg tctgatgtgg atgctaatac tgcaattaag
      661 catgaagaac agtccaacga agatatcccc atagcagaac agtccagcaa ggacatccct
      721 gtgacagcac agcccagtaa ggatgtacat gttgtaaaag agcaaaatcc accacctgca
      781 aggtcagagg acatgccttt tagccccaaa gcatctgttg ctgctatgga agcaaaagaa
      841 cagttgtctg cacaagaact tatggaaagt ggactgcaga ttcagaagtc accagagcct
      901 gaggttttgt caactcagga agacttgttt gaccagagca ataaaacagt atcttctgat
      961 ggttgctcta ctccttcaag ggaggaaggt gggtgttctt tggcttccac tcctgccacc
     1021 actctgcatc tcctgcagct ctctggtcag aggtcccttg ttcaggacag tctttccacg
     1081 aattcttcag atcttgttgc tccttctcct gatgctttcc gatctactcc ttttatcgtt
     1141 cctagcagtc ccacagagca agaagggaga caagataagc caatggacac gtcagtgtta
     1201 tctgaagaag gaggagagcc ttttcagaag aaacttcaaa gtggtgaacc agtggagtta
     1261 gaaaaccccc ctctcctgcc tgagtccact gtatcaccac aagcctcaac accaatatct
     1321 cagagcacac cagtcttccc tcctgggtca cttcctatcc catcccagcc tcagttttct
     1381 catgacattt ttattccttc cccaagtctg gaagaacaat caaatgatgg gaagaaagat
     1441 ggagatatgc atagttcatc tttgacagtt gagtgttcta aaacttcaga gattgaacca
     1501 aagaattccc ctgaggatct tgggctatct ttgacagggg attcttgcaa gttgatgctt
     1561 tctacaagtg aatatagtca gtccccaaag atggagagct tgagttctca cagaattgat
     1621 gaagatggag aaaacacaca gattgaggat acggaaccca tgtctccagt tctcaattct
     1681 aaatttgttc ctgctgaaaa tgatagtatc ctgatgaatc cagcacagga tggtgaagta
     1741 caactgagtc agaatgatga caaaacaaag ggagatgata cagacaccag ggatgacatt
     1801 agtattttag ccactggttg caagggcaga gaagaaacgg tagcagaaga tgtttgtatt
     1861 gatctcactt gtgattcggg gagtcaggca gttccgtcac cagctactcg atctgaggca
     1921 ctttctagtg tgttagatca ggaggaagct atggaaatta aagaacacca tccagaggag
     1981 gggtcttcag ggtctgaggt ggaagaaatc cctgagacac cttgtgaaag tcaaggagag
     2041 gaactcaaag aagaaaatat ggagagtgtt ccgttgcacc tttctctgac tgaaactcag
     2101 tcccaagggt tgtgtcttca aaaggaaatg ccaaaaaaag aatgctcaga agctatggaa
     2161 gttgaaacca gtgtgattag tattgattcc cctcaaaagt tggcaatact tgaccaagaa
     2221 ttggaacata aggaacagga agcttgggaa gaagctactt cagaggactc cagtgttgtc
     2281 attgtagatg tgaaagagcc atctcccaga gttgatgttt cttgtgaacc tttggaggga
     2341 gtggagaagt gctcagattc ccagtcatgg gaggatattg ctccagaaat agaaccatgt
     2401 gctgagaata gattagacac caaggaagaa aagagtgtag aatatgaagg agatctgaaa
     2461 tcagggactg cagaaacaga acctgtagag caagattctt cacagccttc cttaccttta
     2521 gtgagagcag atgatccttt aagacttgac caggagttgc agcagcccca aactcaggag
     2581 aaaacaagta attcattaac agaagactca aaaatggcta atgcaaagca gctaagctca
     2641 gatgcagagg cccagaagct ggggaagccc tctgcccatg cctcacaaag cttctgtgaa
     2701 agttctagtg aaaccccatt tcatttcact ttgcctaaag aaggtgatat catcccacca
     2761 ttgactggtg caaccccacc tcttattggg cacctaaaat tggagcccaa gagacacagt
     2821 actcctattg gtattagcaa ctatccagaa agcaccatag caaccagtga tgtcatgtct
     2881 gaaagcatgg tggagaccca tgatcccata cttgggagtg gaaaagggga ttctggggct
     2941 gccccagacg tggatgataa attatgtcta agaatgaaac tggttagtcc tgagactgag
     3001 gcgagtgaag agtctttgca gttcaacctg gaaaagcctg caactggtga aagaaaaaat
     3061 ggatctactg ctgttgctga gtctgttgcc agtccccaga agaccatgtc tgtgttgagc
     3121 tgtatctgtg aagccaggca agagaatgag gctcgaagtg aggatccccc caccacaccc
     3181 atcaggggga acttgctcca ctttccaagt tctcaaggag aagaggagaa agaaaaattg
     3241 gagggtgacc atacaatcag gcagagtcaa cagcctatga agcccattag tcctgtcaag
     3301 gaccctgttt ctcctgcttc ccagaagatg gtcatacaag ggccatccag tcctcaagga
     3361 gaggcaatgg tgacagatgt gctagaagac cagaaagaag gacggagtac taataaggaa
     3421 aatcctagta aggccttgat tgaaaggccc agccaaaata acataggaat ccaaaccatg
     3481 gagtgttcct tgagggtccc agaaactgtt tcagcagcaa cccagactat aaagaatgtg
     3541 tgtgagcagg ggaccagtac agtggaccag aactttggaa agcaagatgc cacagttcag
     3601 actgagaggg ggagtggtga gaaaccagtc agtgctcctg gggatgatac agagtcgctc
     3661 catagccagg gagaagaaga gtttgatatg cctcagcctc cacatggcca tgtcttacat
     3721 cgtcacatga gaacaatccg ggaagtacgc acacttgtca ctcgtgtcat tacagatgtg
     3781 tattatgtgg atggaacaga agtagaaaga aaagtaactg aggagactga agagccaatt
     3841 gtagagtgtc aggagtgtga aactgaagtt tccccttcac agactggggg ctcctcaggt
     3901 gacctggggg atatcagctc cttctcctcc aaggcatcca gcttacaccg cacatcaagt
     3961 gggacaagtc tctcagctat gcacagcagt ggaagctcag ggaaaggagc cggaccactc
     4021 agagggaaaa ccagcgggac agaacccgca gattttgcct tacccagctc ccgaggaggc
     4081 ccaggaaaac tgagtcctag aaaaggggtc agtcagacag ggacgccagt gtgtgaggag
     4141 gatggtgatg caggccttgg catcagacag ggagggaagg ctccagtcac gcctcgtggg
     4201 cgtgggcgaa ggggccgccc accttctcgg accactggaa ccagagaaac agctgtgcct
     4261 ggccccttgg gcatagagga catttcacct aacttgtcac cagatgataa atccttcagc
     4321 cgtgtcgtgc cccgagtgcc agactccacc agacgaacag atgtgggtgc tggtgctttg
     4381 cgtcgtagtg actctccaga aattcctttc caggctgctg ctggcccttc tgatggctta
     4441 gatgcctcct ctccaggaaa tagctttgta gggctccgtg ttgtagccaa gtggtcatcc
     4501 aatggctact tttactctgg gaaaatcaca cgagatgtcg gagctgggaa gtataaattg
     4561 ctctttgatg atgggtacga atgtgatgtg ttgggcaaag acattctgtt atgtgacccc
     4621 atcccgctgg acactgaagt gacggccctc tcggaggatg agtatttcag tgcaggagtg
     4681 gtgaaaggac ataggaagga gtctggggaa ctgtactaca gcattgaaaa agaaggccaa
     4741 agaaagtggt ataagcgaat ggctgtcatc ctgtccttgg agcaaggaaa cagactgaga
     4801 gagcagtatg ggcttggccc ctatgaagca gtaacacctc ttacaaaggc agcagatatc
     4861 agcttagaca atttggtgga agggaagcgg aaacggcgca gtaacgtcag ctccccagcc
     4921 acccctactg cctccagtag cagcagcaca acccctaccc gaaagatcac agaaagtcct
     4981 cgtgcctcca tgggagttct ctcaggcaaa agaaaactta tcacttctga agaggaacgg
     5041 tcccctgcca agcgaggtcg caagtctgcc acagtaaaac ctggtgcagt aggggcagga
     5101 gagtttgtga gcccctgtga gagtggagac aacaccggtg aaccctctgc cctggaagag
     5161 cagagagggc ctttgcctct caacaagacc ttgtttctgg gctacgcatt tctccttacc
     5221 atggccacaa ccagtgacaa gttggccagc cgctccaaac tgccagatgg tcctacagga
     5281 agcagtgaag aagaggagga atttttggaa attcctcctt tcaacaagca gtatacagaa
     5341 tcccagcttc gagcaggagc tggctatatc cttgaagatt tcaatgaagc ccagtgtaac
     5401 acagcttacc agtgtcttct aattgcggat cagcattgtc gaacccggaa gtacttcctg
     5461 tgccttgcca gtgggattcc ttgtgtgtct catgtctggg tccatgatag ttgccatgcc
     5521 aaccagctcc agaactaccg taattatctg ttgccagctg ggtacagcct tgaggagcaa
     5581 agaattctgg actggcaacc ccgtgaaaat cctttccaga atctgaaggt actcttggta
     5641 tcagaccaac agcagaactt cctggagctc tggtctgaga tcctcatgac tggtggtgca
     5701 gcctctgtga agcagcacca ttcaagtgcc cataacaaag atattgcttt aggggtattt
     5761 gatgtggtgg tgacggaccc ctcatgccca gcctcggtgc tgaagtgtgc tgaagcattg
     5821 cagctgcctg tggtgtcaca agagtgggtg atccagtgcc tcattgttgg ggagagaatt
     5881 ggattcaagc agcatccaaa atataaacac gattatgttt ctcactagta atgaactagt
     5941 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgattac
     6001 ttcgtgcacc tacagcgcat
//
