LOCUS       sequence-285126-        8471 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8471)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 8471)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2475
FEATURES             Location/Qualifiers
     source          1..8471
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 accgccacca tgcggctcgg gggcgggcag ctggtgtcag aggagctgat gaacctgggc
       61 gagagcttca tccagaccaa cgacccgtcg ctgaagctct tccagtgcgc cgtctgcaac
      121 aagttcacga cggacaacct ggacatgctg ggcctgcaca tgaacgtgga gcgcagcctg
      181 tcggaggacg agtggaaggc ggtgatgggg gactcatacc agtgcaagct ctgccgctac
      241 aacacccagc tcaaggccaa cttccagctg cactgcaaga cagacaagca cgtgcagaag
      301 taccagctgg tggcccacat caaggagggc ggcaaggcca acgagtggag gctcaagtgt
      361 gtggccatcg gcaaccccgt gcacctcaag tgcaacgcct gtgactacta caccaacagc
      421 ctggagaagc tgcggctgca cacggtcaac tccaggcacg aggccagcct gaagttgtac
      481 aagcacctgc agcagcatga gagtggtgta gaaggtgaga gctgctacta ccactgcgtt
      541 ctgtgcaact actccaccaa ggccaagctc aacctcatcc agcatgtgcg ctccatgaag
      601 caccagcgaa gcgagagcct gcgaaagctg cagcggctgc agaagggcct tccagaggag
      661 gacgaggacc tggggcagat cttcaccatc cgcaggtgcc cctccacgga cccagaagaa
      721 gccattgaag atgttgaagg acccagtgaa acagctgctg atccagagga gcttgctaag
      781 gaccaagagg gcggagcatc gtccagccaa gcagagaagg agctgacaga ttctcctgca
      841 acctccaaac gcatctcctt cccaggtagc tcagagtctc ccctctcttc gaagcgacca
      901 aaaacagctg aggagatcaa accggagcag atgtaccagt gtccctactg caagtacagt
      961 aatgccgatg tcaaccggct ccgggtgcat gccatgacgc agcactcggt gcaacccatg
     1021 cttcgctgcc ccctgtgcca ggacatgctc aacaacaaga tccacctcca gctgcacctc
     1081 acccacctcc acagcgtggc acctgactgc gtggagaagc tcattatgac ggtgaccacc
     1141 cctgagatgg tgatgccaag cagcatgttc ctcccagcag ctgttccaga tcgagatggg
     1201 aattccaatt tggaagaggc aggaaagcag cctgaaacct cagaggatct gggaaagaac
     1261 atcttgccat ccgcaagcac agagcaaagc ggagatttga aaccatcccc tgctgaccca
     1321 ggctctgtga gagaagactc aggcttcatc tgctggaaga aggggtgcaa ccaggttttc
     1381 aaaacttctg ctgcccttca gacgcatttt aatgaagtgc atgccaagag gcctcagctg
     1441 ccggtgtcag atcgccatgt gtacaagtac cgctgtaatc agtgtagcct ggccttcaag
     1501 accattgaaa agttgcagct ccattctcag taccatgtga tcagagctgc caccatgtgc
     1561 tgtctttgtc agcgcagttt ccgaactttc caggctctga agaagcacct tgagacaagc
     1621 cacctggagc tgagtgaggc tgacatccaa cagctttatg gtggcctgct ggccaatggg
     1681 gacctcctgg caatgggaga ccccactctg gcagaggacc ataccataat tgttgaggaa
     1741 gacaaggagg aagagagtga cttggaagat aaacagagcc caacgggcag tgactctggg
     1801 tcagtacaag aagactcggg ctcagagcca aagagagctc tgcctttcag aaaaggtccc
     1861 aattttacta tggaaaagtt cctagaccct tctcgccctt acaagtgtac cgtctgcaag
     1921 gaatctttca ctcaaaagaa tatcctgcta gtacactaca attctgtctc ccacctgcat
     1981 aagttaaaga gagcccttca agaatcagca accggtcagc cagaacccac cagcagccca
     2041 gacaacaaac cttttaagtg taacacttgt aatgtggcct acagccagag ttccactctg
     2101 gagatccata tgaggtctgt gttacatcaa accaaggccc gggcagccaa gctggaggct
     2161 gcaagtggca gcagcaatgg gactgggaac agcagcagta tttccttgag ctcctccacg
     2221 ccaagtcctg tgagcaccag tggcagtaac acctttacca cctccaatcc aagcagtgct
     2281 ggcattgctc caagctctaa cttactaagc caagtgccca ctgagagtgt agggatgcca
     2341 cccctgggga atcctattgg tgccaacatt gcttcccctt cagagcccaa agaggccaat
     2401 cggaagaaac tggcagatat gattgcatcc aggcagcagc aacaacagca gcagcaacag
     2461 caacaacaac aacaacaaca acaacaacaa gcacaaacgc tggcccaggc ccaggctcaa
     2521 gttcaagctc acctgcagca ggagctgcag caacaggctg ccctgatcca gtctcagctg
     2581 tttaacccca ccctccttcc tcacttcccc atgacaactg agaccctgct gcaactacag
     2641 cagcagcagc acctcctctt ccctttctac atccccagtg ctgagttcca gcttaacccc
     2701 gaggtgagct tgccagtgac cagtggggca ctgacactga ctgggacagg cccaggcctg
     2761 ctggaagatc tgaaggctca ggttcaggtc ccacagcaga gccatcagca gatcttgccg
     2821 cagcagcagc agaaccaact ctctatagcc cagagtcact ctgccctcct tcagccaagc
     2881 cagcaccccg aaaagaagaa caaattggtc atcaaagaaa aggaaaaaga aagccagaga
     2941 gagagggaca gcgccgaggg gggagagggc aacaccggtc cgaaggaaac actgccagat
     3001 gccttgaagg ccaaagagaa gaaagagttg gcaccagggg gtggttctga gccttccatg
     3061 ctccctccac gcattgcttc agatgccaga gggaacgcca ccaaggccct gctggagaac
     3121 tttggctttg agttggtcat ccagtataat gagaacaagc agaaggtgca gaaaaagaat
     3181 gggaagactg accagggaga gaacctggaa aagctcgagt gtgactcctg cggcaagttg
     3241 ttttccaaca tcttgatttt aaagagtcat caagagcacg ttcatcagaa ttactttcct
     3301 ttcaaacagc tcgagaggtt tgccaaacag tacagagacc actacgataa actgtaccca
     3361 ctgaggcccc agaccccaga gccaccacca cctccccctc caccccctcc acccccactt
     3421 ccggcagcgc cgcctcagcc ggcgtccaca ccagccatcc ccgcatcagc cccacccatc
     3481 acctcaccta caattgcacc ggcccagcca tcagtgccgc tcacccagct ctccatgccg
     3541 atggagctgc ccatcttctc gccgctgatg atgcagacga tgccgctgca gaccttgccg
     3601 gctcagctac ccccgcagct gggacctgtg gagcctctgc ctgcggacct ggcccaactc
     3661 taccagcatc agctcaatcc aaccctgctc cagcagcaga acaagaggcc tcgcaccagg
     3721 atcacagatg atcagctccg agtcttgcgg caatattttg acattaacaa ctcccccagt
     3781 gaagagcaaa taaaagagat ggcagacaag tccgggttgc cccagaaagt gatcaagcac
     3841 tggttcagga acactctctt caaagagagg cagcgtaaca aggactcccc ttacaacttc
     3901 agtaatcctc ctatcaccag cctggaggag ctcaagattg actcccggcc cccttcgccg
     3961 gaacctccaa agcaggagta ctggggaagc aagaggtctt caagaacaag gtttacggac
     4021 taccagctga gggtcttaca ggacttcttc gatgccaatg cttacccaaa ggatgatgaa
     4081 tttgagcaac tctctaattt actgaacctt ccaacccgag tgatagtggt gtggtttcag
     4141 aatgcccgac agaaggccag gaagaattat gagaatcagg gagagggcaa agatggagag
     4201 cggcgtgagc ttacaaatga tagatacatt cgaacaagca acttgaacta ccagtgcaaa
     4261 aaatgtagcc tggtgtttca gcgcatcttt gatctcatca agcaccagaa gaagctgtgt
     4321 tacaaggatg aggatgagga ggggcaggac gacagccaaa atgaggattc catggatgcc
     4381 atggaaatcc tgacgcctac cagctcatcc tgcagtaccc cgatgccctc acaggcttac
     4441 agcgccccag caccatcagc caataataca gcttcctccg ctttcttgca gcttacagcg
     4501 gaggctgagg aactggccac cttcaattca aaaacagagg caggcgatga gaaaccaaag
     4561 ctggcggaag ctcccagtgc acagccaaac caaacccaag aaaagcaagg acaaccaaag
     4621 ccagagctgc agcagcaaga gcagcccgag cagaagacca acactcccca gcagaagctc
     4681 ccccagctgg tgtccctgcc ttcgttgcca cagcctcctc cacaagcgcc ccctccacag
     4741 tgccccttac cccagtcgag ccccagtcct tcccagctct cccacctgcc cctcaagccc
     4801 ctccacacat caactcctca acagctcgca aacctacctc ctcagctaat cccctaccag
     4861 tgtgaccagt gtaagttggc atttccgtca tttgagcact ggcaggagca tcagcagctc
     4921 cacttcctga gcgcgcagaa ccagttcatc cacccccagt ttttggacag gtccctggat
     4981 atgcctttca tgctctttga tcccagtaac ccactcctgg ccagccagct gctctctggg
     5041 gccatacctc agattccagc aagctcagcc acttctcctt caactccaac ctccacaatg
     5101 aacactctca agaggaagct ggaggaaaag gccagtgcaa gccctggcga aaacgacagt
     5161 gggacaggag gagaagagcc tcagagagac aagcgtttga gaacaaccat cacaccggaa
     5221 caactagaaa ttctctacca gaagtatcta ctggattcca atccgactcg aaagatgttg
     5281 gatcacattg cacacgaggt gggcttgaag aaacgtgtgg tacaagtctg gtttcagaac
     5341 acccgagctc gggaaaggaa aggacagttc cgggctgtag gcccagcgca ggcccacagg
     5401 agatgccctt tttgcagagc gctcttcaaa gccaagactg ctcttgaggc tcatatccgg
     5461 tcccgtcact ggcatgaagc caagagagct ggctacaacc taactctgtc tgcgatgctc
     5521 ttagactgtg atgggggact ccagatgaaa ggagatattt ttgacggaac tagcttttcc
     5581 cacctacccc caagcagtag tgatggtcag ggtgtccccc tctcacctgt gagtaaaacc
     5641 atggaattgt cacccagaac tcttctaagc ccttcctcca ttaaggtgga agggattgaa
     5701 gactttgaaa gcccctccat gtcctcagtt aatctaaact ttgaccaaac taagctggac
     5761 aacgatgact gttcctctgt caacacagca atcacagata ccacaactgg agacgagggc
     5821 aacgcagata acgacagtgc aacgggaata gcaactgaaa ccaaatcctc ttctgcaccc
     5881 aacgaagggt tgaccaaagc ggccatgatg gcaatgtctg agtatgaaga tcggttgtca
     5941 tctggtctgg tcagcccggc cccgagcttt tatagcaagg aatatgacaa tgaaggtaca
     6001 gtggactaca gtgaaacctc aagccttgca gatccctgct ccccgagtcc tggtgcgagt
     6061 ggatctgcag gcaaatctgg tgacagcgga gatcggcctg ggcagaaacg ttttcgcact
     6121 caaatgacca atctgcagct gaaggtcctc aagtcatgct ttaatgacta caggacaccc
     6181 actatgctag aatgtgaggt cctgggcaat gacattggac tgccaaagag agtcgttcag
     6241 gtctggttcc agaatgcccg ggcaaaagaa aagaagtcca agttaagcat ggccaagcat
     6301 tttggtataa accaaacgag ttatgaggga cccaaaacag agtgcacttt gtgtggcatc
     6361 aagtacagcg ctcggctgtc tgtacgtgac catatctttt cccaacagca tatctccaaa
     6421 gttaaagaca ccattggaag ccagctggac aaggagaaag aatactttga cccagccacc
     6481 gtacgtcagt tgatggctca acaagagttg gaccggatta aaaaggccaa cgaggtcctt
     6541 ggactggcag ctcagcagca agggatgttt gacaacaccc ctcttcaggc ccttaacctt
     6601 cctacagcat atccagcgct ccagggcatt cctcctgtgt tgctcccggg cctcaacagc
     6661 ccctccttgc caggctttac tccatccaac acagctttaa cgtctcctaa gccgaacttg
     6721 atgggtctgc ccagcacaac tgttccttcc cctggcctcc ccacttctgg attaccaaat
     6781 aaaccgtcct cagcgtcgct gagctcccca accccagcac aagccacgat ggcgatgggc
     6841 cctcagcaac ccccccagca gcagcagcag cagcagcaac cacaggtgca gcagcctccc
     6901 ccgccgccag cagcccagcc gccacccaca ccacagctcc cactgcaaca gcagcagcaa
     6961 cgcaaggaca aagacagtga gaaagtaaag gagaaggaaa aggcacacaa agggaaaggg
     7021 gaacccctgc ctgtccccaa gaaggagaaa ggagaggccc ccacggcaac tgcagccacg
     7081 atctcagccc cgctgcccac catggagtat gcggtagacc ctgcacagct gcaggccctg
     7141 caggccgcgt tgacttcgga ccccacagca ttgctcacaa gccagttcct tccttacttt
     7201 gtaccaggct tttctcctta ttatgctccc cagatccctg gcgccctgca gagcgggtac
     7261 ctgcagccta tgtatggcat ggaaggcctg ttcccctaca gccctgcact gtcgcaggcc
     7321 ctgatggggc tgtccccagg ctccctactg cagcagtacc agcaatacca gcagagtctg
     7381 caggaggcaa ttcagcagca gcagcagcgg caactacagc agcagcagca gcaaaaagtg
     7441 cagcagcagc agcccaaagc aagccaaacc ccagtccccc ccggggctcc ttccccagac
     7501 aaagaccctg ccaaagaatc ccccaaacca gaagaacaga aaaacacccc ccgtgaggtg
     7561 tcccccctcc tgccgaaact ccctgaagag ccagaagcag aaagcaaaag tgcggactcc
     7621 ctctacgacc ccttcattgt tccaaaggtg cagtacaagt tggtctgccg caagtgccag
     7681 gcgggcttca gcgacgagga ggcagcgagg agccacctga agtccctctg cttcttcggc
     7741 cagtctgtgg tgaacctgca agagatggtg cttcacgtcc ccaccggcgg cggcggcggt
     7801 ggcagtggcg gcggcggcgg cggtggcggc ggcggcggcg gcggcggctc gtaccactgc
     7861 ctggcgtgcg agagcgcgct ctgtggggag gaagctctga gtcaacatct cgagtcggcc
     7921 ttgcacaaac acagaacaat cacgagagca gcaagaaacg ccaaagagca ccctagttta
     7981 ttacctcact ctgcctgctt ccccgatcct agcaccgcat ctacctcgca gtctgccgct
     8041 cactcaaacg acagcccccc tcccccgtcg gccgccgccc cctcctccgc ttccccccac
     8101 gcctccagga agtcttggcc gcaagtggtc tcccgggctt cggcagcgaa gcccccttct
     8161 tttcctcctc tctcctcatc ttcaacggtt acctcaagtt catgcagcac ctcaggggtt
     8221 cagccctcga tgccaacaga cgactattcg gaggagtctg acacggatct cagccaaaag
     8281 tccgacggac cggcgagccc ggtggagggt cccaaagacc ccagctgccc caaggacagt
     8341 ggtctgacca gtgtaggaac ggacaccttc agattgtagt aatgaactag tccgtaactt
     8401 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgacga gcattggact
     8461 ctgataatga a
//
