LOCUS       sequence-285132-         998 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2517
FEATURES             Location/Qualifiers
     source          1..998
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctgccgc cggggccagg cctgtggagc tgggcttcgc cgagtcggcg
       61 ccggcgtggc gactgcgcag cgagcagttc cccagcaagg tgggcgggcg gccggcatgg
      121 ctgggcgcgg ccgggctgcc ggggccccag gccctggcct gcgagctgtg cggccgcccg
      181 ctctccttcc tgctgcaggt gtatgcgccg ctgcctggcc gcccggacgc cttccaccgc
      241 tgcatcttcc tcttctgctg ccgcgagcag ccgtgctgtg ccggcctgcg agtttttagg
      301 aatcaactac ccaggaaaaa cgatttttac tcatatgagc caccttctga gaatcctccc
      361 ccagaaacag gagaatcagt gtgtctccag cttaagtctg gtgctcatct ctgcagggtt
      421 tgtggctgtt taggccccaa aacgtgctcc agatgccaca aagcatatta ctgcagcaag
      481 gagcatcaga ccctagactg gagattggga cataagcagg cttgtgcaca accagatcat
      541 ctggaccata taattccaga ccacaacttc ctttttccag aatttgaaat tgtaatagaa
      601 acagaagatg agattatgcc tgaggttgtg gaaaaggaag attactcaga gattataggg
      661 agcatgggtg aagcacttga ggaagaactg gattccatgg caaaacatga atccagggaa
      721 gataaaattt ttcagaagtt taaaactcag atagcccttg aaccagaaca gattcttaga
      781 tatggcagag gtattgcccc catctggatt tctggtgaaa atattcctca agaaaaggat
      841 attccagatt gcccctgtgg tgccaagaga atattggaat tccagttttc agacacggtc
      901 tattagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
      961 tgtggaaagg acgatggtgg ctttccctac gcatcagt
//
