LOCUS       sequence-285141-        1967 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2618
FEATURES             Location/Qualifiers
     source          1..1967
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcctccag gcttccgacg gcctggtcct gtgaaccagt gacctttgaa
       61 gatgtaacac tgggttttac cccggaagag tggggactgc tggacctcaa acagaagtcc
      121 ctgtacaggg aagtgatgct ggagaactac aggaacctgg tctcagtgga gtatcctgag
      181 ctccagctgg accctaaatt ggatcctctt cctgctgaga gtcccctaat gaacattgag
      241 gttgttgagg tcctcacact gaaccaggag gtggctggtc cccggaatgc ccagatccag
      301 gccctatatg ctgaagatgg aagcctgagt gcagatgccc ccagtgagca ggtccaacag
      361 cagggcaagc atccaggtga ccctgaggcc gcgcgccaga ggttccggca gttccgttat
      421 aaggacatga caggtccccg ggaggccctg gaccagctcc gagagctgtg tcaccagtgg
      481 ctacagccta aggcacgctc caaggagcag atcctggagc tgctggtgct ggagcagttc
      541 ctaggtgcac tgcctgtgaa gctccggaca tgggtggaat cgcagcaccc agagaactgc
      601 caagaggtgg tggccctggt agagggtgtg acctggatgt ctgaggagga agtacttcct
      661 gcaggacaac ctgccgaggg caccacctgc tgcctcgagg tcactgccca gcaggaggag
      721 aagcaggagg atgcagccat ctgcccagtg acagtgctcc ctgaggagcc agtgaccttc
      781 caggatgtgg ctgtggactt cagccgggag gagtgggggc tgctgggccc gacacagagg
      841 accgagtacc gcgatgtgat gctggagacc tttgggcacc tggtctctgt ggggtgggag
      901 actacactgg aaaataaaga gttagctcca aattctgaca ttcctgagga agaaccagcc
      961 cccagcctga aagtacaaga atcctcaagg gattgtgcct tgtcctctac attagaagat
     1021 accttgcagg gtggggtcca ggaagtccaa gacacagtgt tgaagcagat ggagtctgct
     1081 caggaaaaag accttcctca gaagaagcac tttgacaacc gtgagtccca ggcaaacagt
     1141 ggtgctcttg acacaaacca agtttcgctc cagaaaattg acaaccctga gtcccaggca
     1201 aacagtggcg ctcttgacac aaaccaagtt ttgctccaca aaattcctcc tagaaaacga
     1261 ttgcgcaaac gtgactcaca agttaaaagt atgaaacata attcacgtgt aaaaattcat
     1321 cagaagagct gtgaaaggca aaaggccaag gaaggcaatg gttgtaggaa aaccttcagt
     1381 cggagtacta aacagattac gtttataaga attcacaagg ggagccaagt ttgccgatgc
     1441 agtgaatgtg gtaaaatatt ccggaaccca agatactttt ctgtgcataa gaaaatccat
     1501 accggagaga ggccctatgt gtgtcaagac tgtgggaaag gatttgttca gagctcttcc
     1561 ctcacacagc atcagagagt tcattctgga gagagaccat ttgaatgtca ggagtgtggg
     1621 aggaccttca atgatcgctc agccatctcc cagcacctga ggactcacac tggcgctaag
     1681 ccctacaagt gtcaggactg tggaaaagcc ttccgccaga gctcccacct catcagacat
     1741 cagaggactc acaccgggga gcgcccatat gcatgcaaca aatgtggaaa ggccttcacc
     1801 cagagctcac accttattgg gcaccagaga acccacaata ggacaaagcg aaagaagaaa
     1861 cagcctacct catagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     1921 tatatatctt gtggaaagga cgaaatcatc agtcgcgaca aagattc
//
