LOCUS       sequence-285144-        2513 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2619
FEATURES             Location/Qualifiers
     source          1..2513
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaaattcc gaaactgctg ccagcccgtg gtacgctgca aggcggcggt
       61 ggtggcggta tcccagccgg tggtggtcgt gttcatcgcg gtccggatag cccagccggt
      121 caagttccga cccgtcgtct gctgctgctg cgtggtccac aagatggcgg tccgggccgt
      181 cgccgcgaag aagccagcac cgccagtcgt ggtccgggtc caagtctgct ggccccacgc
      241 accgatcagc caagtggcgg cggcggtggt ggtggcgatg atttttttct cgttctcctg
      301 gatccagttg gcggtgatgt tgaaactgct ggttctggcc aagctgctgg tccggttctg
      361 cgtgaagaag ctgaagaagg tccaggtctg caaggtggtg aatctggtgc taacccggct
      421 ggtccgaccg ctctgggtcc gcgttgtctc tccgcggttc ccactccggc cccgatctcc
      481 gcccccggcc ccgccgcggc cttcgcgggc acagtcacta tccacaacca ggacctgctg
      541 ttgcgctttg agaacggcgt cctcaccctg gccacgcccc caccacacgc ctgggagcca
      601 ggggccgctc ctgcccagca gcccgggtgt ctgatcgccc cgcaagctgg gttcccgcat
      661 gccgcgcacc cgggtgactg cccagagctg ccgccagacc tcctgctagc tgagccggcc
      721 gaacccgcgc cagctccggc gcctgaggag gaggcggagg gcccggccgc cgccctgggc
      781 ccccgcggac cgctgggctc cggcccaggc gtggtgctgt acttgtgccc cgaggcgcag
      841 tgcgggcaaa ccttcgccaa gaagcaccag ctgaaggtgc acctgctgac gcacagcagc
      901 agccagggcc agaggccctt caaatgcccc ctgggtggct gcggctggac cttcaccacc
      961 tcttacaagc tcaagaggca cctgcagtcg cacgacaaac tgcggccctt tggctgcccg
     1021 gcagagggct gtggcaagag cttcaccaca gtgtacaacc tcaaggcgca catgaagggc
     1081 catgagcagg agaactcatt caaatgcgag gtgtgcgagg agagcttccc cacgcaggcc
     1141 aaactcagcg cccaccagcg cagccacttc gaaccggaga ggccttacca gtgcgcgttt
     1201 tctggctgca agaagacatt tatcacagtg agtgctctgt tttcccataa ccgcgcccat
     1261 ttcagggaac aggaactgtt ttcctgctct tttcctggct gcagcaaaca atatgacaag
     1321 gcttgtaggc tgaaaattca cctgcggagt cacaccggcg agagaccttt cctttgtgac
     1381 tttgatggct gtggctggaa cttcactagc atgtccaaac tcttaaggca caaaagaaag
     1441 cacgacgatg accggaggtt catgtgccct gtggaaggct gtgggaaatc tttcacgagg
     1501 gcggaacatc tgaaaggcca cagcataacc cacctgggca caaagccttt cgtgtgtcct
     1561 gtggcaggct gctgtgccag gttctctgct cgcagtagcc tctacattca ctccaagaaa
     1621 cacctgcagg atgtggacac ttggaaaagc cgttgcccga tctcctcttg taataaactc
     1681 ttcacatcca agcacagcat gaagacgcac atggttaaaa ggcataaggt gggccaggat
     1741 ctcttagctc agctagaagc agcaaattct ctcacaccca gcagtgaact taccagccag
     1801 agacagaatg atctcagtga tgcagagata gtgtctctct tctctgatgt acctgacagt
     1861 acttctgctg cattgctgga cacagcattg gtgaactctg gaatcttgac tattgatgtg
     1921 gcttctgtga gctcgactct ggcagggcac ctccctgcta ataataataa ttccgtaggg
     1981 caggctgtgg accctccgtc cttgatggcc accagcgacc ctcctcaaag tctggatacc
     2041 tctctctttt ttggaacggc ggccactggt tttcagcaga gctccttaaa tatggatgag
     2101 gtctcaagtg taagtgtggg gccattggga tctctggact ctttggccat gaaaaactcc
     2161 agtccagagc ctcaggcttt gacacccagc agtaagctaa cagtggacac agatgctctg
     2221 actccttcga gcaccctttg tgaaaacagt gtctcagaac tactgacacc aaccaaagcg
     2281 gagtggaacg tacatcctga ctctgacttc tttggacagg agggagaaac ccagtttgga
     2341 ttccccaatg cagcaggaaa ccatggttct cagaaagaaa cagatcttat cactgtgact
     2401 ggcagctcat ttttggtata gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     2461 tggctttata tatcttgtgg aaaggacgaa agatatgcgt catacgtcgg ctg
//
