LOCUS       sequence-285162-        8249 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8249)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 8249)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2699
FEATURES             Location/Qualifiers
     source          1..8249
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggcggc ggcgggcggc ggcagttgcc ccgggcctgg ctccgcgcgg
       61 ggccgcttcc cgggccggcc gcggggcgcc ggcgggggcg ggggccgcgg cggacggggc
      121 aacggggccg aaagagtgcg ggtagctctg cggcgcggcg gtggcgcgac ggggccgggc
      181 ggagccgagc ccggggagga cacggccctg ctccgtttgc tggggctccg ccggggcctg
      241 cgccggctcc gccgcctgtg ggccggcccg cgggtccagc ggggccgggg acggggtcgg
      301 ggccggggct ggggcccgag tcgaggctgc gtgccggagg aggagagcag tgacggggaa
      361 tccgacgagg aggagtttca gggttttcat tcagatgaag atgtggcccc cagttccctg
      421 cgctctgcgc tccgatccca gcgaggtcga gcgccccgag gtcggggtcg caagcataag
      481 acgacccccc ttcctcctcc tcgcctagca gatgtggctc ctaccccccc aaagacccct
      541 gcccggaaac ggggtgagga aggcacagaa cggatggtgc aggcactgac tgaacttctc
      601 cggcgggccc aggcacccca agcaccccgg agccgggcat gtgagccctc caccccccgg
      661 cggtctcggg gacggccccc aggacggcca gcaggcccct gcaggaggaa gcagcaagca
      721 gtagtggtgg cagaagcagc tgtgacaatc cccaaacctg agcccccacc tcctgtggtt
      781 ccagtgaaac atcagactgg cagctggaaa tgcaaggagg ggcccggtcc aggacctggg
      841 acccccaggc gtggaggaca gtcaagccgt ggaggccgtg gaggcagggg ccgcggccga
      901 ggtggtgggc tcccctttgt gatcaagttt gtttcaaggg ccaaaaaagt aaagatggga
      961 caattgtcct tgggactcga atcaggtcaa ggtcaaggtc aacatgagga aagttggcag
     1021 gatgtccccc aaagaagagt tggatctgga cagggaggga gcccttgctg gaaaaagcag
     1081 gaacagaagc tggatgacga ggaagaagag aagaaagaag aagaagaaaa agacaaggag
     1141 ggagaagaga aggaagaaag agctgtagct gaggagatga tgccagctgc ggaaaaggaa
     1201 gaggcaaagc tgccaccacc gcctctgact cctccagccc cttcacctcc tccacccctc
     1261 ccaccccctt cgacatctcc tccaccccca ctctgccctc caccaccacc cccagtgtcc
     1321 ccaccacctc taccatcccc tccaccgcct cctgcccaag aggagcagga ggaatcccct
     1381 cctcctgtgg tcccagctac gtgctccagg aagaggggcc ggcctcccct gactcccagc
     1441 cagcgggcgg agcgggaagc tgctcgggca gggccagagg gcacctctcc tcccactcca
     1501 acccccagca ccgccacggg aggccctccg gaagacagtc ccaccgtggc ccccaaaagc
     1561 accaccttcc tgaagaatat ccggcagttt attatgcctg tggtgagtgc ccgctcctcc
     1621 cgtgtcatca agacaccccg gcgatttatg gatgaagacc cccccaaacc cccaaaggtg
     1681 gaggtctcac ctgtcctgcg acctcccatt accacctccc cacctgttcc ccaggagcca
     1741 gcaccagtcc cctctccacc acgtgcccca actcctccat ctaccccagt tccactccct
     1801 gagaagagac ggtccatcct aagggaaccc acatttcgct ggacctcact gacccgggag
     1861 ctgccccctc ctcccccagc ccctccacct cccccggccc cctccccacc ccctgctcct
     1921 gccacctcct cccggaggcc cctactcctt cgggcccctc agtttacccc aagcgaagcc
     1981 cacctgaaga tctacgaatc ggtgcttact cctcctcctc ttggggctcc tgaagcccct
     2041 gagccagagc ctcctcctgc cgatgactct ccagctgagc ctgagcctcg ggcagtgggc
     2101 cgcaccaacc acctcagcct gcctcgattc gcccctgtgg tcaccactcc tgttaaggcc
     2161 gaggtgtccc ctcacggggc tccagctctg agcaacgggc cacagacaca ggctcagcta
     2221 ctgcagcccc tgcaggcctt gcaaacccag ctcctgcccc aggcactacc gccaccacag
     2281 ccacagctgc agccaccgcc gtcaccacag cagatgcctc ccctggaaaa agcccggatt
     2341 gcgggcgtgg gttccttgcc gctgtctggg gtagaggaga agatgttcag cctcctcaag
     2401 agagccaaag tgcagctatt caagatcgat cagcagcagc agcagaaggt ggcagcttcc
     2461 atgccgctga gccctggagg gcagatggag gaggtggccg gggctgtcaa gcagatctcc
     2521 gacagaggcc ctgtccggtc tgaagatgag tcggtggaag ctaagagaga gcggccctca
     2581 ggtcccgagt cccctgtgca aggtccccgc atcaaacatg tctgccgtca tgctgctgtg
     2641 gccctgggtc aggcccgggc catggtgcct gaagatgtcc ctcgcctcag tgccctccct
     2701 ctccgggatc ggcaggacct cgccacagag gatacatcat cggcgtccga gactgagagt
     2761 gtcccgtcac ggtcccggcg gggaaaggtg gaggcagcag gccctggggg agaatcagag
     2821 cccacaggtt ctggagggac cctggcccac acaccccggc gctcactgcc ctcccatcac
     2881 ggcaagaaga tgcgcatggc tcgatgtgga cactgtcggg gctgcctacg tgtgcaggac
     2941 tgtgggtcct gtgtcaactg cctagacaag cccaagtttg ggggccctaa caccaagaag
     3001 cagtgctgtg tataccggaa gtgtgacaaa atagaggctc ggaagatgga acgactggct
     3061 aaaaaaggcc ggacgatagt gaagacgctg ttgccctggg attccgatga atctcctgag
     3121 gcctcccctg gtcctccagg cccacgccgg ggggcgggag ctggggggcc ccgggaggag
     3181 gtggtggccc acccagggcc cgaggagcag gactccctcc tgcagcgcaa gtcagctcgg
     3241 cgctgcgtca aacagcgacc ctcctatgat atcttcgagg attcggatga ctcggagccc
     3301 gggggccccc ctgctcctcg gcgtcggacc ccccgagaaa atgagctgcc actgccagaa
     3361 cctgaggagc agagccggcc ccgcaaacct accctgcagc ctgtgttgca gctcaaggcc
     3421 cgaaggcgcc tggacaagga tgctttggcc cctggcccct ttgcttcttt tcccaatggc
     3481 tggactggaa agcagaagtc tcccgatggt gtgcaccgcg tccgtgtgga ttttaaggag
     3541 gattgtgatt tagagaacgt gtggctgatg gggggcctga gtgtgctcac ctctgtgcca
     3601 gggggccccc cgatggtgtg cttgctgtgt gccagcaaag gactccacga gctggtgttc
     3661 tgtcaagtct gctgtgaccc attccaccca ttctgcctgg aggaggccga gcggcccctg
     3721 ccccagcatc acgacacctg gtgctgccgt cgctgcaaat tctgccacgt ctgtggacgc
     3781 aaaggtcgtg gatccaagca cctcctggag tgcgagcgct gccgccatgc ataccacccg
     3841 gcctgtctgg ggcccagcta tccaacccgg gccacgcgca aacggcgcca ctggatctgt
     3901 tcagcctgtg tgcgctgtaa gagctgtggg gcaactccag gcaagaactg ggacgtcgag
     3961 tggtctggag attacagcct ctgccccagg tgcacccagc tatatgagaa aggaaactac
     4021 tgcccgatct gtacacgctg ctatgaagac aacgactatg agagcaagat gatgcagtgc
     4081 gcacagtgcg atcactgggt gcatgccaag tgcgaggggc tctcagatga agactacgag
     4141 atcctttcag gactgccaga ctcggtgctg tacacctgcg gaccgtgtgc tggggcagcg
     4201 cagccccgct ggcgagaggc cctgagcggg gccctccagg ggggcctgcg ccaggtgctc
     4261 cagggcctgc tgagctccaa ggtggtgggc ccactgctgc tctgcaccca gtgtgggcca
     4321 gatgggaagc aactgcaccc aggaccctgc ggcctgcaag ctgtgagtca gcgcttcgag
     4381 gatggccact acaagtctgt gcacagcttc atggaggaca tggtgggcat cctcatgcgg
     4441 cactcggagg agggagagac cccggaccgc cgggctggag gccagatgaa ggggctcctg
     4501 ctgaagctgc tagaatctgc gttcggctgg ttcgacgccc acgaccccaa gtactggcga
     4561 cggagtaccc ggctgccaaa cggagtcctt cccaatgcgg tgttgccccc atccctggat
     4621 catgtctatg cgcagtggag acagcaggaa ccagagaccc cagaatcagg gcagcctcca
     4681 ggggatccct cagcagcatt ccagggcaag gatccggctg ccttctcaca cctggaggac
     4741 ccccgtcagt gtgcactctg cctcaaatac ggggatgcag actccaagga ggcggggcgg
     4801 ctcttgtaca tcgggcagaa cgagtggaca cacgtcaact gtgccatctg gtcggcggaa
     4861 gtcttcgagg agaacgacgg ctccctcaag aatgtgcatg ctgctgtggc ccgagggagg
     4921 cagatgcgct gcgagctctg cctgaagcct ggcgccacgg tgggctgctg cctgtcctcc
     4981 tgcctcagca acttccactt catgtgtgcc cgggccagct actgcatctt ccaggatgac
     5041 aagaaagtct tctgccagaa acacactgat ctcctggatg gcaaggaaat tgtgaacccc
     5101 gatggttttg atgttctccg ccgagtctat gtggacttcg agggcatcaa cttcaagcgg
     5161 aagttcttga cggggcttga acccgatgcc atcaacgtgc tcattggttc catccgcatt
     5221 gactccctgg gtactctgtc tgatctctcg gactgcgagg gacggctctt ccccattggc
     5281 taccagtgct cccgtctgta ctggagcaca gtggatgctc ggaggcgctg ctggtatcgg
     5341 tgccgaattc tggagtatcg gccatggggg ccgagggaag agccagctca cctggaggct
     5401 gcagaggaga accagaccat tgtgcacagc cccgcccctt cctcagagcc cccaggtggt
     5461 gaggaccccc cactggacac agatgttctt gtccctggag ctcctgagcg ccactcgccc
     5521 attcagaacc tggaccctcc actgcggcca gattcaggca gcgcccctcc tccagccccc
     5581 cgttcttttt cgggggctcg aatcaaagtg cccaactact cgccatcccg gaggcccttg
     5641 gggggtgtct cctttggccc cctgccctcc cctggaagtc catcttcact gacccaccac
     5701 atccccacag tgggagaccc ggacttccca gctcccccca gacgttcccg tcgtcccagc
     5761 cctttggctc ccaggccgcc tccatcacgg tgggcctccc ctcctctaaa aacctcccct
     5821 cagctcaggg tgccccctcc tacctcagtc gtcacagccc tcacacctac ctcaggggag
     5881 ctggctcccc ctggcccggc cccatctcca ccaccccctg aagacctggg cccagacttc
     5941 gaggacatgg aggtggtgtc aggactgagt gctgctgacc tggacttcgc ggccagcctg
     6001 ctggggactg agcccttcca ggaagagatt gtagccgctg gggccatggg gagcagccac
     6061 gggggcccgg gggacagctc cgaggaggag tccagcccca cctcccgcta catccacttc
     6121 cctgtgactg tggtgtccgc ccctggtctg gcccccagcg ctacccctgg agccccccgc
     6181 attgaacagc tggacggcgt ggacgacggc actgacagtg aggctgaggc ggtgcagcag
     6241 cctcggggcc agggcacgcc tccttcgggg ccaggagtag tccgggcagg ggtccttggg
     6301 gctgcagggg acagggcccg gcctcctgag gacctgccat cggaaattgt ggattttgtg
     6361 ttgaagaacc tagggggtcc tggggatgga ggtgctggcc ctagagagga gtcactcccc
     6421 ccggcgcctc ccctggctaa tggcagccag ccctcccaag gcctgaccgc cagcccagct
     6481 gaccccaccc gcacatttgc ctggctccca ggggccccag gggtccgggt gttaagcctt
     6541 ggccctgccc ctgagccccc caaacccgcc acatccaaaa tcatacttgt caacaagctg
     6601 gggcaagtat ttgtgaagat ggctggggag ggtgaacctg tcccaccccc agtgaagcag
     6661 ccacctttgc cccccaccat ttcccccacg gctcccacct cctggactct gcccccaggc
     6721 cccctcctcg gcgtgctgcc cgtggtcgga gtggtccgcc ctgccccgcc cccgccaccc
     6781 cctcccctga cgctggtgct gagcagtggg ccagccagcc cgccccgcca ggccatccgc
     6841 gtcaagaggg tgtccacttt ctccggccgg tccccgccag cacctccccc atacaaagcc
     6901 ccccggctgg atgaagatgg agaggcctca gaggataccc ctcaggttcc agggcttggc
     6961 agtggcgggt ttagccgtgt gaggatgaaa acccccacag tgcgtggggt ccttgacctg
     7021 gatcggcctg gggagcccgc tggggaagaa agtcctgggc ccctccagga acggtcccct
     7081 ttgctgccac ttccggaaga tggtcctccc caggtccccg atggtccccc agacctgctg
     7141 cttgagtccc agtggcacca ctattcaggt gaggcttcga gctctgagga agagcctcca
     7201 tccccagatg ataaagagaa ccaggcccca aaacggactg gcccacatct gcgcttcgag
     7261 atcagcagtg aggatgggtt cagcgttgag gcagagagct tggagggggc gtggagaact
     7321 ctgatcgaga aagtgcaaga ggcccgaggg catgcccgac tcagacatct ctcctttagt
     7381 ggaatgagtg gggcgagact cctgggcatc caccatgatg ctgtcatctt cctggccgag
     7441 cagctccccg gagcccagcg ttgccagcac tataagttcc gttaccacca gcagggagag
     7501 ggccaggagg agccgcccct gaatccccat ggggctgctc gggcagaggt ctatctccgg
     7561 aagtgcacct ttgacatgtt caacttcctg gcctcccagc accgggtgct ccctgagggg
     7621 gccacctgtg atgaggaaga ggatgaggtg cagctcaggt caaccagacg tgccaccagc
     7681 ctggagctgc ccatggccat gcgttttcgt caccttaaga agacgtccaa agaagctgtg
     7741 ggtgtctaca gatcagccat ccacgggcga ggcctgttct gtaagcgcaa catcgacgcg
     7801 ggggagatgg tcatcgagta ctctggcatt gtcatccgct cggtgttgac tgacaagcgg
     7861 gagaagttct acgatgggaa gggcatcggg tgctatatgt tccgcatgga tgactttgat
     7921 gtagtggacg ccacgatgca tggcaatgcc gcccgcttca tcaaccactc ctgtgagccc
     7981 aactgcttct ctcgggtcat ccacgtggag ggccagaaac acattgttat cttcgccctg
     8041 cgccgcatcc tgcgtggtga ggagctcacc tacgactaca agttccccat cgaggatgcc
     8101 agcaacaagc tgccctgcaa ctgtggcgcc aagcgctgcc gtcggttcct taactagtaa
     8161 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     8221 gacgaaagga gtgtgccgac tgactatca
//
