LOCUS       sequence-285168-        8051 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8051)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 8051)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2746
FEATURES             Location/Qualifiers
     source          1..8051
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctgtgtg ggaggccgag cagctgggtg gcctccagcg gggggacctg
       61 ctcacacccc ctgcccctga tggcgacggg cgcacggccc cccttggcca gccccctggg
      121 gcccagctgt actgcccggc ctgcttggtc acctgccact ctcaggaggc cttcgagaac
      181 cactgcgcat cctcggagca cgcacagatg gtggccttcg accaggccct gccctgggag
      241 caccgttccc cacccccggg actctccaag ttcgagctct gcccaaagcc tgacctctgt
      301 gagtatgggg acgcctgcac caaggcacac tcagcacagg agctgcagga gtgggtccgg
      361 cgcacgcagg ctgtggagct gcgggggcag gcggcctggc aggacgggct ggtgccctac
      421 caggagcggc tgctggccga gtaccagcgc agcagcagtg aggtccttgt gctggcagag
      481 acccttgatg gagtgcgtgt cacctgcaac cagcccctga tgtaccaggc ccaggagagg
      541 aagacccagt acagctggac gtttgccgtc cactctgagg agcccctgct acacgtggcc
      601 ctgctgaagc aggagccagg agccgacttc tctctggtgg ctcccggcct cccgccaggc
      661 cggctctacg cacggggtga gcgcttccgt gtgcccagct ccactgccga cttccaggtg
      721 ggagtgcgtg tgcaggctgc ctcgttcggc acctttgagc aatgggtggt cttcgacttt
      781 ggccgccggc cggtgctgct acaaaagctg gggctgcagc tgggccaggg gcgtcgccca
      841 ggaccctgca ggaatctggc gctcggccac cctgaggaga tggagcgctg gcacactggc
      901 aaccgccacg tggtgcctgg cgtggagcgg acggccgagc agacggccct gatggccaag
      961 tacaagggcc ctgccctggc cctggagttc aaccgcagca gcgtggcctc gggccccatc
     1021 tcaccaacca actatcggca gaggatgcac cagtttctct atgaggagga ggcggctcag
     1081 cagcagctgg tggccaagct gaccctgcgg ggccaggtgt tcctgaagac ggcattgcag
     1141 acgccagcgc tgaacatgct cttcgcgcct ccgggagcac tgtacgcaga ggtccccgtc
     1201 ccctcctccc tgatgccaga cacagaccag ggcttcctgc tgggccgggc ggtcagcaca
     1261 gccctggtgg cccctgtacc tgcacccgac aatacggtgt tcgaggtgcg gctggagagg
     1321 cgggccagct cagagcaggc gctgtggctg ctgcttccgg cccgctgctg cttggccctg
     1381 gggctgcagc ctgaggcccg cctggtcctg gaggtgcagt tccagattga cccgatgacc
     1441 ttccgcctct ggcaccaggc agtggacaca ctgcctgagg agcagctggt ggtgcccgac
     1501 ttgcccacct gcgccctgcc cagaccttgg tctgtcccac ccttgcggcg tggcaaccgc
     1561 aagcaggagc tggccgtggc gctcatcgcg ggctggggcc ctggggatgg gaggcgtgtc
     1621 cccccgctac tcatctatgg cccctttggc accggcaaga cctacacgct ggccatggcc
     1681 tccctggagg tcatccggag gcctgaaacc aaggtgctca tctgcacaca caccaacagt
     1741 gccgccgaca tctacatccg ggagtatttc cacagccacg tcagcggcgg ccaccccgag
     1801 gccactcctc tccgtgtgat gtacacggac cggccgctga gccagacgga cccagtcacg
     1861 ctgcagtact gttgcctgac cgacgaccgc caggctttcc gcccgcccac acgggcagag
     1921 ctggcgcggc accgcgtggt ggtcaccacc acctcccagg cccgtgagct cagggtgccg
     1981 gtcggcttct tctcccacat tctcatcgat gaggcggccc agatgctgga gtgcgaggcc
     2041 ctcaccccgc tggcctatgc ctcgcacggc acccgcctcg tgctggcggg cgaccacatg
     2101 caggtcacac cccggctgtt cagtgtggcc agggcccggg cggccgagca cacgctgctg
     2161 caccgcctct tcctgtgcta ccagcaggag actcacgagg tggcgcggca gagccgcctg
     2221 gtcttccacg agaactaccg ctgcacggac gccattgtca gcttcatctc gcggcacttc
     2281 tacgtggcca agggcaaccc catccacgcc aggggcaagg ttccgcccca cccccggcac
     2341 tacccgctca tgttctgcca cgtggcgggc agcccagacc gggacatgtc catggcgtcc
     2401 tggctgaatc tggctgagat tgcgcaggtc gtcgagaagg tgcaggaggc ctacaacacc
     2461 tggcccagct gctggggcgg ccgcgagcag aggtgcatct gtgtcgtttc ccacggtgcc
     2521 caggtcagtg cactgaggca ggagctgagg aggcgggacc taggccaggt gtctgtcggc
     2581 agttttgaga tcctgccagg gcggcagttc cgggtcgtgg tgctcagcac tgtgcacacc
     2641 tgccagagcc tgctcagccc tggggcactg gcccctgagt tcttcaccga cgcccgcgtg
     2701 ctcaacaccg tcctgacccg cgcccagtcc cagctggtgg tagtggggga cgccgtggcc
     2761 ctctgctcct tcggggcctg cggcaagctc tgggagagct tcatccgtga gtgcgtggag
     2821 cggcacagtg tctgccccga gggcctgtcc atggagcagg tcgagcaggg tgtggcgcag
     2881 agacggcgct ggcctccccg aggcacacag gctggggcag cggggaactg ggaggctgcc
     2941 ccagagccag taggggacct ggccgaggag caggcggctg tggtgacggc catggtgaag
     3001 gcagagccgg gagatgaggc tctgagccca gcatcccgtg acatcacggc aaccacagcg
     3061 cagacggagg ctgcggcagc accagcagga gacgcagtga aggaagacgt ggtgcccggg
     3121 gcctgtgcgg caggagcggc tgctgcagcg ggcgtggagt ccacggaggc tgaggatgca
     3181 gaggctgact tctggccgtg ggacggggag ctcaacgctg acgacgccat cctacgggag
     3241 cttctggacg agagccagaa ggtgatggtg accgtcgggg aggacgggct gctggacact
     3301 gtcgccaggc ccgagtccct gcagcaggcc cggctgtacg agaacctgcc cccggctgcg
     3361 ctacggaagc tgctgcacgc ggagcccgag cggtaccgcc actgctcttt cgtgccagag
     3421 accttcgagc gggcgtcagc catcccgctg gacgatgcct cctcgggccc catccaggtc
     3481 aggggccgcc tggactgtgg gatggccttc gccggggatg aggtgctggt gcagctcctt
     3541 tcgggagaca aggcgcccga ggggcggctt cggggccgcg tgctgggcgt gctgaagagg
     3601 aagaggcacg agctggcgtt tgtgtgccgc atggacacgt gggacccgcg catcatggtc
     3661 cccatcaatg gctccgtgac caagatcttc gtggccgagc tgaaggaccc atcgcaggtc
     3721 cccatctaca gcctccggaa gggccggctg cagcgtgtgg ggcttgagag gctcaccgcc
     3781 gaggcccggc acagccggct cttctgggtc caaattgtcc tgtggcggca aggcttctac
     3841 tacccgctgg gcatcgtccg ggaggtgctg cctgaggcca gcacctggga gcagggcctc
     3901 cgcatcctcg gcctggagta cagcttgagg gtgcccccgt cggaccaggc caccatcacc
     3961 aaggtgctgc agaaatacca cacggagctt ggccgggttg ccggccgccg agaggactgc
     4021 cgcgccttct tgaccttcac tgtggacccc cagggcgcct gcaacctcga tgatgccctc
     4081 agtgtccgag acctgggtcc caggtgcgag gtggctgtgc acatcactga tgtggccagc
     4141 ttcgtgccca gggacggggt gctggacgtg gaggcgcgaa ggcagggcgc tgcgttctat
     4201 gcccccggca gggagccagt gcccatgctg ccggccagcc tctgccagga cgtcctcagc
     4261 ctcctgcctg gccgggaccg cctggccatc tccctgttcc tcaccatgga gaaggccagt
     4321 ggccagctga agagcctgcg ctttgcaccc tccgtggtcc agtctgaccg ccagctgtcc
     4381 tacgaggagg cggaggaggt gatcaggcag cacccgggtg ccggccgtga gctgccggcc
     4441 cgcctggact ccgtggacgc ctgcgtcgtg gccgcgtgct acttctctcg gctgctgcgc
     4501 cggcaccgcc tgcggtccga ctgcttctat gagcagccgg acgaggacgg caccctgggc
     4561 ttccgcgcgg cccacatcat ggtgaaggag tacatgattc agtttaatag gctcgtggct
     4621 gagttcctgg tgggcagcga gtgcacgcgg acggtcacgc ctctgcggtg gcagccagca
     4681 ccccgcagcc agcagctcaa ggccctgtgt gagaagcatg gggaccgggt gcccctgtca
     4741 ctgcacctcg gccaccacct gcacggcggc gggggcagtc cccccgacac gcggctgcac
     4801 ctcctggcct ccctctggaa gcaggtccag tttgctgccc gcacccagga ctacgaacag
     4861 atggtggact tggtcaccac ggacgacatg cacccattcc tggctcctgc aggccgcgac
     4921 ctccgcaagg ccctggagcg ctcggcgttc ggccgctgcg cccggggcca ccagcagcag
     4981 ggcggccact actcgctgca ggtggactgg tacacgtggg ccacctcgcc catccgcagg
     5041 tacctggacg tggtgttgca gcggcagatc ctgctggcgc tgggccatgg gggctctgcc
     5101 tactctgcca gggacatcga tgggctctgc caggccttca gcctccagca cgcacttgcc
     5161 cagagctatc agcggcgggc gcgcagcctg cacctggccg tgcagctcaa ggcccagcct
     5221 ctggacaagc tgggcttcgt ggtggacgtg gaggcgggct cccgctgctt ccggctgctc
     5281 ttccccagca accgggagac gctgcctgac ccctgccccg tcccctacgg ctccctgcag
     5341 ctggccgagc acccccacgc cctggcaggc cggccgggcc tgcggctcct gtggcggcgc
     5401 cgtgtctact cagcgcaggg atccagcccg cccctgccac tgcctggcac tgtgccggac
     5461 ccacacaccc tggccgtgga gacggccctg tggaagcagc tgctggagct ggtggagctg
     5521 cagcgctggc cggaggcggc tgctctcatc caggagaagg gcgaggcgtc ccagcggcgg
     5581 gagctggtgc aggtgcagcg gagccactgt ggccatttcc tggaggtggc ccgggagctg
     5641 ggcagtgggg acaccctgca ggtgcagctc ggcaccagcc tgcagcacgg cttcctggta
     5701 ccgagccctc agctctggac ggtggcaccg ggcttcagcc tctgcctgga gcacgtggag
     5761 cggcccggag actgcttctc aggccgtgtg taccgggccc cgagggaccg gtaccgcgac
     5821 gtggatgagt acgcctgcgt gtgggaacca ttctgcgccc tggagtcggc caccggcgcg
     5881 gttgccgaga atgactccgt cacacttcag cacctgagtg tctcctggga ggcgtcacgg
     5941 acgccgcagg ggcagctgca gggcgccttc cgcctggagg ccgccttcct cgaggagaac
     6001 tgtgccgaca tcaacttcag ctgctgctac ctctgcatcc ggctcgaggg gctgccggct
     6061 cccacggcca gcccacgccc tgggcccagc agcctcggcc ctggcctgaa tgttgacccc
     6121 ggcacgtata cctgggtggc ccacgggcag acgcaggact gggaccagga gcgccgggca
     6181 gaccggcagg aggctcccag acgggtgcac ctcttcgtcc accacatggg catggagaag
     6241 gttccggaag aggtgctgag gccgggcacc ctgttcaccg ttgagctgct gcccaagcag
     6301 cttcctgatc tccgcaagga ggaagccgtg cgtggactag aggaggcgtc cccgctggtc
     6361 accagcatcg cactgggccg gcctgtcccg cagcccctct gcagagtgat ccccagcagg
     6421 ttcctggagc ggcagaccta caacatcccc ggaggccgcc acaagctgaa ccccagccag
     6481 aacgtggcgg tcagggaggc tctggagaag cctttcacgg tcattcaggg cccaccaggt
     6541 acagggaaga cgatcgtggg cctccacatc gtattctggt ttcataaatc aaaccaggag
     6601 caggtgcagc ccggaggccc cccccgtggg gagaagcggc tggggggtcc ctgcatcttg
     6661 tactgcggcc cctccaacaa gtcggtggat gtcctggcag gactgctcct gagaaggatg
     6721 gagctgaagc ccctccgtgt gtacagtgag caggctgagg ccagcgagtt cccagtgccg
     6781 cgtgtgggca gcaggaagct gctcaggaag agcccccggg aggggaggcc gaaccagagc
     6841 ctcaggagca tcaccctgca ccaccggatc cggcaggccc ccaaccctta ctcgtcggaa
     6901 atcaaggcct ttgacacccg gctgcagaga ggggagctct tctccaggga ggacctggtc
     6961 tggtacaaga aggtcttgtg ggaggctcgg aagttcgagc tggaccggca tgaggtcatc
     7021 ctctgcacct gctcctgtgc agcctctgcc agcctcaaaa tcctggacgt gaggcagatc
     7081 cttgttgacg aggcaggcat ggccacggaa cctgaaaccc tcatccccct ggtgcagttc
     7141 ccacaggccg agaaggtggt tcttctcgga gaccacaagc agctgcggcc tgtggtcaag
     7201 aatgagcggc tgcaaaacct gggtctggac cggtctctgt tcgagcggta ccacgaggac
     7261 gcacatatgc tggacactca gtaccgcatg catgagggca tctgtgcctt cccctctgtg
     7321 gcgttctaca agagcaagct gaagacgtgg cagggcctga ggaggccgcc cagtgtcctg
     7381 ggccacgctg gcaaggagag ctgccctgtc atctttggcc acgtgcaggg ccacgagcgg
     7441 agcctgctgg tgtccacgga cgaagggaat gagaactcca aggccaacct ggaggaggtg
     7501 gctgaggtgg tccgtatcac caagcagctg accctgggga ggaccgtaga gccccaggac
     7561 atcgccgtcc tcacgcccta caacgcgcag gcctctgaga tcagcaaggc ccttcggcga
     7621 gagggcatcg ccggggtggc cgtgtcctcc atcaccaaga gccaggggag cgagtggcgc
     7681 tatgtgctgg tgagcaccgt ccgcacctgt gccaagagcg acctggacca gcggcccacc
     7741 aagagctggc tcaagaagtt tctgggcttc gttgtggacc ccaaccaagt gaatgtggct
     7801 gtcacgcggg cccaggaggg gctctgcctg atcggagacc acctccttct gcgctgctgc
     7861 cccctctggc gtagcctcct ggacttctgc gaggctcagc agaccctcgt gcctgccggc
     7921 caggtgcgcg tctgcaggag gccaactatg ccttcctagt aatgaactag tccgtaactt
     7981 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgaata tgaacgtatg
     8041 gcatagcgtg a
//
