LOCUS       sequence-285174-        4652 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4652)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 4652)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2777
FEATURES             Location/Qualifiers
     source          1..4652
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    886..897
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
ORIGIN
        1 accgccacca tggagccggg gtccgacgat ttcctaccgc caccggagtg cccggtgttc
       61 gagcctagct gggccgagtt ccgagaccct cttggctaca tcgcgaaaat caggcccatc
      121 gcagagaaat cgggcatttg caagatccgc ccacccgcgg actggcagcc accctttgct
      181 gtggaagtgg acaacttcag gtttaccccc cgaatccaga ggctgaatga gctagagatt
      241 gtggtggagg aaggtggtta tgaagctatc tgcaaggacc gtcggtgggc tcgggtagcc
      301 cagcgcctca actatccacc aggcaaaaat attggctcct tgctacgctc ccactacgaa
      361 cgcattgttt atccctatga aatgtaccag tctggagcca accttgtgca gtgtaacaca
      421 cgtccatttg ataatgagga gaaggacaag gaatacaaac cccacagcat ccccctacga
      481 cagtctgtgc agccttccaa gttcaacagc tatggccggc gggccaagag actgcagcct
      541 gatccggaac ccacagagga agacattgag aagaatccag agctgaaaaa gctacagatc
      601 tatggggcag gccccaagat gatgggcctg ggcctcatgg ccaaagacaa gactctgcgg
      661 aagaaagata aggaggggcc tgagtgtccc cccacagtag tggtgaagga ggagttaggt
      721 ggggatgtga aggtggagtc aacatcgcct aagaccttcc tggagagcaa ggaggagctg
      781 agtcacagcc cagaaccctg caccaagatg accatgaggc tacggaggaa ccacagcaat
      841 gcccagttta ttgagtcata tgtctgccgg atgtgttctc gaggggatga ggatgacaag
      901 ctcctgctgt gtgatggctg tgatgacaac taccacatct tctgcctgct gcctcctctg
      961 cctgagatcc ccaagggtgt ctggcggtgc ccaaagtgtg tcatggcgga gtgtaagcgg
     1021 cccccagaag cctttggctt tgagcaggct acccgggaat acactctgca gagctttggc
     1081 gagatggccg actcctttaa agctgactac ttcaacatgc ccgtgcatat ggtgcccaca
     1141 gaacttgtgg agaaggagtt ctggaggctg gtaaatagca ttgaggaaga tgtgactgtt
     1201 gagtatggag ctgacatcca ttccaaagaa tttggcagcg gtttccctgt cagtgacagt
     1261 aaacggcacc taacccccga agaggaggag tatgctacca gtggttggaa cctaaatgtg
     1321 atgccggtgt tggaacagtc tgtactgtgc cacatcaatg cagatatctc tggcatgaag
     1381 gtgccctggc tctacgtggg catggtcttc tcagcctttt gctggcatat tgaggatcac
     1441 tggagttact ccattaacta cctccactgg ggtgagccga agacctggta tggggtgccc
     1501 tcacttgcag cagaacattt ggaagaagtg atgaagaagc tgacacctga actatttgat
     1561 agccagcctg acctcctgca ccaacttgtc accctcatga atcccaacac cctcatgtcc
     1621 catggtgtgc cagttgtccg cacaaaccag tgtgcaggag agtttgtcat caccttcccc
     1681 cgtgcttacc acagcggctt caaccaaggc tacaactttg ccgaggctgt caacttttgc
     1741 actgctgact ggttgcctgc tgggcgccag tgcattgagc actaccgccg gctccggaga
     1801 tactgcgtct tctcccatga ggagcttatc tgcaagatgg ctgcctgccc agagaagcta
     1861 gacctgaacc tggcggcagc tgtgcataag gagatgttca tcatggtgca agaagagcgg
     1921 cgtctacgaa aggccctgct ggagaagggt atcacagagg ctgagcgaga ggctttcgag
     1981 ctgctcccag atgatgagcg ccagtgtatc aagtgcaaga ctacgtgttt cctgtcagcc
     2041 ctggcctgct acgactgccc agacggcctt gtctgccttt cccacatcaa tgatctctgc
     2101 aagtgctcca gtagccggca gtacctgcgg tatcggtata ccttggatga gcttcctgcc
     2161 atgctgcata agctgaaggt tcgggctgag tcctttgaca cctgggccaa caaagtgcga
     2221 gtggccctgg aggtggagga tgggcggaag cgcagccttg aagaactgag ggcactagag
     2281 tctgaagccc gtgagcggag gtttcctaat agtgagctgc tgcagcaact aaagaactgc
     2341 ctgagtgagg cagaggcttg cgtgtcccga gctctgggac tggtcagcgg ccaggaagct
     2401 ggcccccaca gggtggctgg tctacagatg accctgactg agctccgggc ctttctggac
     2461 cagatgaaca acctgccttg cgccatgcac cagattgggg atgtcaaggg tgttctggaa
     2521 caggtggagg cctaccaggc tgaggctcgt gaggccctgg cctcactgcc ctccagtcca
     2581 gggctactgc agtccctgtt ggagaggggg cggcagctgg gggtggaggt gcctgaggcc
     2641 cagcagctcc agcggcaggt ggaacaggcg cgatggctgg atgaggtgaa acgcacactg
     2701 gccccctcag cccgaagggg caccttggct gtcatgcgag gactgttggt cgcgggtgcc
     2761 agtgtagccc ctagccctgc tgtggataaa gcccaggccg agctgcagga actgctgacc
     2821 attgctgaac gctgggagga gaaagcccac ctctgcctgg aggccaggca gaagcatcca
     2881 ccagccacac ttgaggccat aatccgtgaa gcggaaaaca tccctgttca cctgcccaac
     2941 atccaggctc tcaaggaggc tcttgctaag gcccgggcct ggattgctga tgttgatgag
     3001 atccaaaatg gtgaccacta cccctgcctg gatgacttgg agggcctagt agctgtgggc
     3061 cgggacctac ctgtggggct ggaggagctg agacagctag agctacaggt actgacagcg
     3121 cactcctgga gggagaaggc ctccaagacc ttcctcaaga aaaattcttg ctacacgctg
     3181 ctggaggttc tctgcccatg tgcagatgcc ggctcagaca gcaccaagcg cagccggtgg
     3241 atggagaagg agctggggtt gtacaaatct gacacagagc tgctggggct gtctgcgcag
     3301 gacctcaggg acccaggctc tgtgatcgtg gccttcaagg agggggaaca gaaggagaag
     3361 gagggtatcc tgcagctgcg tcgcaccaat tcggccaagc ccagtccact ggcatcatcg
     3421 agcacggcct cctctacaac ctctatctgt gtgtgtgggc aggtgctggc tggggcggga
     3481 gctctgcagt gtgacctgtg tcaggactgg ttccatgggc ggtgtgtgtc agtgcctcgc
     3541 ctcctcagct ctccgaggcc caatcccacc tcatccccac tgctggcctg gtgggaatgg
     3601 gacaccaaat tcctgtgtcc actgtgtatg cgctcaaggc gcccgcgcct ggagaccatc
     3661 ctggcactgc tggtagccct gcagagactg cctgtgcggc tgcccgaggg cgaggccctg
     3721 cagtgcctca cagagagggc catcagctgg caaggccgcg ccaggcaggc tctggcctct
     3781 gaagatgtga ctgctctttt gggacggctg gctgagctcc gccaacggct acaggctgaa
     3841 cctagacctg aggagcctcc taactaccct gcagcccctg cttctgaccc cctcagagag
     3901 ggcagtggca aggatatgcc taaggtccag ggcttactgg agaatggaga cagtgtgacc
     3961 agtcctgaga aggtagcccc ggaggagggc tcagatctgg agctgctgtc ctcgctgttg
     4021 ccacagttga ctggccctgt gttggaactg cctgaggcaa cccgggcccc cttggaggag
     4081 ctcatgatgg agggggacct gctcgaggtg accctggatg agaaccacag catatggcag
     4141 ctgctgcagg ctggacagcc cccagacctg gagaggatcc gcacacttct ggagctggag
     4201 aaggcagagc gtcacgggag tcgggctcgg ggccgggccc tggagaggcg gcggcggcgg
     4261 aaggtggatc ggggtgggga gggcgatgac ccagcccgag aggagctaga gccaaagagg
     4321 gtacggagct cagggccaga ggctgaggag gtccaggagg aggaagagct ggaggaggag
     4381 actgggggtg agggcccccc tgcacccatc cccaccactg gcagccccag cacccaggag
     4441 aaccagaatg gcttggaacc ggcggaaggg accacttcag gcccctcggc ccctttctcc
     4501 actctgactc cccggctgca tctgccctgc ccacagcagc cgcctcagca acagttgtag
     4561 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     4621 aaggacgagg ccaataacaa ccacagaagt cc
//
