LOCUS       sequence-285180-        2249 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2895
FEATURES             Location/Qualifiers
     source          1..2249
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggccgccaa catgtacagg gtcggagact acgtctactt tgagaactcc
       61 tccagcaacc catacctgat ccggagaatc gaggagctca acaagacggc caatgggaac
      121 gtggaggcca aagtggtgtg cttctaccgg aggcgggaca tctccagcac cctcatcgcc
      181 ctggccgaca agcacgcaac cctgtcagtc tgctataagg ccggaccggg ggcggacaac
      241 ggcgaggaag gggaaataga agaggaaatg gagaacccgg aaatggtgga cctgcccgag
      301 aaactaaagc accagctgcg gcatcgggag ctgttcctct cccggcagct ggagtctctg
      361 cccgccacgc acatcagggg caagtgcagc gtcaccctgc tcaacgagac cgagtcgctc
      421 aagtcctacc tggagcggga ggatttcttc ttctattctc tagtctacga cccacagcag
      481 aagaccctgc tggcagataa aggagagatt cgagtaggaa accggtacca ggcagacatc
      541 accgacttgt taaaagaagg cgaggaggat ggccgagacc agtccaggtt ggagacccag
      601 gtgtgggagg cgcacaaccc actcacagac aagcagatcg accagttcct ggtggtggcc
      661 cgctctgtgg gcaccttcgc acgggccctg gactgcagca gctccgtccg acagcccagc
      721 ctgcacatga gcgccgcagc tgcctcccga gacatcaccc tgttccacgc catggatact
      781 ctccacaaga acatctacga catctccaag gccatctcgg cgctggtgcc gcagggcggg
      841 cccgtgctct gcagggacga gatggaggag tggtctgcat cagaggccaa ccttttcgag
      901 gaagccctgg aaaaatatgg gaaggatttc acggacattc agcaagattt tctcccgtgg
      961 aagtcgctga ccagcatcat tgagtactac tacatgtgga agaccaccga cagatacgtg
     1021 cagcagaaac gcttgaaagc agctgaagct gagagcaagt taaagcaagt ttatattccc
     1081 aactataaca agccaaatcc gaaccaaatc agcgtcaaca acgtcaaggc cggtgtggtg
     1141 aacggcacgg gggcgccggg ccagagccct ggggctggcc gggcctgcga gagctgttac
     1201 accacacagt cttaccagtg gtattcttgg ggtcccccta acatgcagtg tcgtctctgc
     1261 gcatcttgtt ggacatattg gaagaaatat ggtggcttga aaatgccaac ccggttagat
     1321 ggagagaggc caggaccaaa ccgcagtaac atgagtcccc acggcctccc agcccggagc
     1381 agcgggagcc ccaagtttgc catgaagacc aggcaggctt tctatctgca cacgacgaag
     1441 ctgacgcgga tcgcccggcg cctgtgccgt gagatcctgc gcccgtggca cgctgcgcgg
     1501 cacccctacc tgcccatcaa cagcgcggcc atcaaggccg agtgcacggc gcggctgccc
     1561 gaagcctccc agagcccgct ggtgctgaag caggcggtac gcaagccgct ggaagccgtg
     1621 cttcggtatc ttgagaccca cccccgcccc cccaagcctg accccgtgaa aagcgtgtcc
     1681 agcgtgctca gcagcctgac gcccgccaag gtggcccccg tcatcaacaa cggctccccc
     1741 accatcctgg gcaagcgcag ctacgagcag cacaacgggg tggacggcaa catgaagaag
     1801 cgcctcttga tgcccagtag gggtctggca aaccacggac aggccaggca catgggacca
     1861 agccggaacc tcctgctcaa cgggaagtcc taccccacca aagtgcgcct gatccggggg
     1921 ggctccctgc ccccagtcaa gcggcggcgg atgaactgga tcgacgcccc ggatgacgtg
     1981 ttctacatgg ccacagagga gaccaggaag atccgcaagc tgctctcatc ctcggaaacc
     2041 aagcgtgctg cccgccggcc ctacaagccc atcgccctgc gccagagcca ggccctgccg
     2101 ccgcggccac cgccacctgc gcccgtcaac gacgagccca tcgtcatcga ggactagtaa
     2161 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     2221 gacgacgata ttccaactgc gaaagtcga
//
