LOCUS       sequence-285186-        4130 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4130)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 4130)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0331
FEATURES             Location/Qualifiers
     source          1..4130
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaggagaa cgaggtggag agcagcagcg acgcggcccc tgggcctggc
       61 cggcccgagg agccctctga gagcggcctg ggtgtgggca cctcagaagc cgtgtccgcc
      121 gacagcagcg acgccgcggc cgccccgggg caggcagagg ccgatgactc tggcgtgggg
      181 caaagctcgg accgcggcag ccgttctcag gaggaggtat ctgagagcag ctcgagcgca
      241 gaccccctgc ctaatagcta cctccctgat tcatcgtctg tgtctcatgg gccagtggca
      301 ggggtgacag gcggtccccc agcacttgtg cactctagtg cactcccaga ccccaacatg
      361 ctggtgtccg actgcacagc ttcctcctcg gacctgggct cggccatcga caagatcatc
      421 gagtccacca tcgggcccga cctcatccag aactgcatca ctgtgaccag tgctgaggat
      481 ggcggggccg agaccacacg gtacctgatc ctacagggcc cagatgatgg agcccccatg
      541 acatcaccaa tgtccagttc caccttggcc cacagcctag cagccattga ggccctggca
      601 gatggcccca catccacatc cacatgcctg gaggcacagg gtgggcccag ctccccggtg
      661 cagctgcccc cagcctccgg tgccgaagag ccggacctgc agagcctgga ggccatgatg
      721 gaggtggtgg tggtgcagca gttcaaatgc aagatgtgcc agtaccggag cagcaccaag
      781 gccacactgc tgcgccacat gcgggaacgc cacttccgtc cagtagcagc agccgcagca
      841 gcagctggta aaaaaggacg tctacggaag tggagcacct ccaccaagag ccaagaggaa
      901 gagggaccag aggaggagga cgatgatgac attgtagacg ctggagccat tgatgacctg
      961 gaggaggata gcgactataa tccagctgag gatgagcccc gaggccggca gcttcggctc
     1021 cagcgcccca cccccagtac cccaaggccc cgaaggagac ctggccggcc ccggaagctg
     1081 ccccgcctgg agatctcaga cctcccagat ggtgtggaag gagagcctct agtgagttcc
     1141 cagagtggac agagccctcc agagccacag gatcccgagg ctcccagctc ctcaggccca
     1201 ggacacctgg tggccatggg caaggtgagc aggacccctg tggaagctgg tgtgagccag
     1261 tcagatgcag agaacgcagc cccctcctgc ccggatgagc atgacactct gccccggcgc
     1321 cgaggtcgac cttccaggcg cttcctaggc aagaaatacc gcaagtacta ttacaagtcg
     1381 cccaaaccac ttttgaggcc cttcctgtgc cgcatctgtg gttctcgctt tctgtcccac
     1441 gaggacctgc gcttccacgt caactcccat gaggctggcg atccccagct cttcaagtgc
     1501 ctgcagtgca gctatcgttc ccgccgctgg tcctcgctca aggagcacat gttcaaccac
     1561 gtgggcagca agccctacaa gtgtgacgag tgcagctaca ccagtgtcta ccggaaggac
     1621 gtcattcggc acgccgctgt gcacagccgg gaccggaaga agaggccaga tccgactcca
     1681 aagctgagct ctttcccctg ccctgtgtgt ggccgtgtgt accccatgca gaaaagactc
     1741 acgcagcaca tgaagacgca cagcactgag aagccccaca tgtgtgacaa gtgtggaaag
     1801 tcctttaaga agcgctacac cttcaaaatg cacctgctca cgcacatcca ggctgttgcc
     1861 aaccgcaggt tcaagtgtga gttctgtgag tttgtttgtg aagacaagaa ggcactgctg
     1921 aaccaccagt tgtcccacgt cagtgacaag cccttcaaat gcagcttttg tccctaccgc
     1981 accttccgag aggacttctt gctgtcccat gtggctgtca agcacacagg ggccaagccc
     2041 ttcgcctgtg agtactgcca cttcagcaca cggcacaaga agaacctgcg cctgcacgta
     2101 cggtgccgac acgcaagcag cttcgaggaa tgggggaggc gccaccctga ggagcccccc
     2161 tcccgccgtc gccccttctt ctctctgcag cagattgagg agctgaagca gcagcacagt
     2221 gcggcccctg gaccacctcc cagttcccca ggacctcctg agataccccc agaggcgaca
     2281 actttccagt catctgaggc tccctcattg ctctgttctg acaccctggg cggcgccacc
     2341 atcatctacc agcaaggagc tgaggagtcg acagcgatgg ccacgcagac agccttggat
     2401 cttctgctga acatgagtgc tcagcgggaa ctggggggca cagccctgca ggtggctgtg
     2461 gtgaagtcgg aagatgtgga agcagggtta gcatcccctg gtgggcagcc ctcccctgaa
     2521 ggtgccactc cacaggtggt caccctccac gtggcagagc cagggggcgg tgcagcagcc
     2581 gagagccagc taggccctcc tgacctaccg cagatcaccc tggcacctgg tccatttggt
     2641 gggactggct acagtgtcat cacagcaccc cctatggagg agggaacatc agctcctggc
     2701 acaccttaca gcgaggagcc cgcaggagag gcagcccagg ctgtggttgt gagtgacacc
     2761 ctaaaagaag ctggcaccca ctacatcatg gctactgatg gtacccagtt gcaccacatt
     2821 gagctcaccg cagatggctc catctccttc ccaagtccag atgctctggc ctctggtgcc
     2881 aaatggcccc tgctgcagtg tgggggactg cccagagacg gccctgagcc cccatctcca
     2941 gccaagaccc actgcgtagg ggactcccag agctctgcct cctcacctcc tgcaaccagc
     3001 aaagccctgg gcctggcagt gcccccgtca ccgccatctg cagccactgc tgcatcaaag
     3061 aagttttcct gcaagatctg tgccgaggcc ttccctggcc gagctgagat ggagagtcac
     3121 aagcgggccc acgctgggcc tggtgccttc aagtgccccg actgcccctt cagtgcccgc
     3181 cagtggcccg aggtccgggc gcacatggca cagcactcaa gcctacggcc ccaccagtgt
     3241 agccagtgca gctttgcctc caagaacaag aaggacctgc gtcggcacat gctgactcac
     3301 acaaaggaga agccttttgc atgccacctc tgcgggcagc gtttcaaccg taacgggcac
     3361 ctcaagttcc acatccagcg gctgcacagt cctgatggga ggaagtcagg aacccctaca
     3421 gcccgggccc ctacccagac cccaacccag accatcatcc tgaacagtga tgacgaaaca
     3481 ctggccaccc tgcacactgc actccagtcc agtcacgggg tcctgggccc agagcggcta
     3541 cagcaggcac tgagccagga acacatcatc gttgcccagg aacagacagt gaccaatcag
     3601 gaggaagccg cctacatcca agagatcacc acggcagatg gccagaccgt acagcacctg
     3661 gtgacctccg acaaccaggt gcagtatatc atctcccagg atggtgtcca gcacctgctc
     3721 ccccaggaat atgttgtggt ccctgaaggc catcacatcc aggtacagga gggccagatc
     3781 acacacatcc agtatgaaca aggagccccg ttccttcagg agtcccagat ccagtatgtg
     3841 cctgtgtccc caggccagca gcttgtcaca caggctcaac ttgaggctgc agcacactca
     3901 gctgtcacag cagtggctga tgctgccatg gcccaagccc agggcctgtt tggtacagac
     3961 gagacagtgc ccgaacacat tcaacagctg cagcaccagg gcatcgagta cgacgtcatc
     4021 accctggccg atgactagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
     4081 ctttatatat cttgtggaaa ggacgaaccg tccggtcttt attacccgtc
//
