LOCUS       sequence-285192-        2624 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0477
FEATURES             Location/Qualifiers
     source          1..2624
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcccagca gggccagcag gggcagatgg caagtggaga cagcaatctc
       61 aacttcagga tggtcgcaga gatccagaac gtagagggtc agaacctgca ggagcaggtt
      121 tgccctgagc ccattttcag gttcttcaga gaaaacaagg tggagattgc aagtgcaata
      181 acaaggccat ttcctttcct tatgggcctc cgagaccgct ccttcatctc cgagcagatg
      241 tatgaacatt ttcaagaagc ttttagaaac ctggtcccag tgacaagagt gatgtattgt
      301 gtactcagtg aactggagaa gacatttggc tggtcacatc tggaagcatt gttcagcagg
      361 attaacctga tggcctatcc tgatttaaac gagatttaca gaagcttcca gaatgtatgc
      421 tatgaacact cacctctcca aatgaataat gtaaacgatt tagaagatag acccagatta
      481 ctaccatatg gtaaacaaga gaacagcaat gcctgtcatg aaatggatga tatagcagtg
      541 cctcaggaag ccttgagctc ctcgccaagg tgtgagccag gtttctcttc agagtcttgt
      601 gagcagttag ctctcccaaa ggctggtgga ggagatgctg aagatgcacc cagcctacta
      661 ccaggtgggg gagtgtcctg taaacttgct atacaaatag atgaaggaga atcagaagaa
      721 atgcccaagt tactgcctta tgatacagaa gttctagaaa gcaacgggat gatagatgcg
      781 gcaaggacat acagcacagc accaggggag aaacagggag aggaggaagg caggaacagt
      841 cccagaaaaa gaaaccaaga caaggagaag taccaagaga gtccagaggg aagagacaaa
      901 gagacctttg atctaaaaac tccccaagtc actaatgaag gagaaccaga gaaggggctc
      961 tgtctactac caggtgaagg agaagagggc agtgatgact gttcagaaat gtgtgatgga
     1021 gaagagcccc aggaagcctc tagctcccta gcaagatgtg ggtcagtttc ttgtttatct
     1081 gcagagacct ttgatctaaa gactccccaa gtcactaatg aaggagaacc agagaaggag
     1141 ctcagtctac taccaggtga aggagaagtg tctagtgaac tagaaaatca cccaatgaat
     1201 gaagaaggag aatcagaaga gcttgcttct agcctgctat atgataatgt accaggagcg
     1261 gagcaatcag catatgaaaa tgagaagtgt tcctgtgtca tgtgtttctc agaagaggtg
     1321 ccaggaagcc cagaagcaag gacggaaagt gatcaagcgt gtggcacaat ggatactgtg
     1381 gatattgcaa acaactccac tttgggaaaa cccaagagga aaagaagaaa aaagaggggg
     1441 catggctgga gcagaatgag aatgagaagg caggaaaaca gccaacaaaa tgataatagc
     1501 aaagccgacg gccaggtggt ctccagtgaa aagaaggcga acgtgaatct gaaagacctt
     1561 tccaagatta gggggagaaa gagaggcaaa cctggaaccc gcttcactca gagtgacaga
     1621 gctgcacaga aaagagtccg atcaagagct tcaagaaagc acaaagatga aactgtggat
     1681 tttaaggctc ctttgcttcc agtgacctgt ggtggggtga agggaatttt acataagaag
     1741 aaattgcagc aaggaatctt ggtgaagtgt atacagactg aggatggaaa atggttcacc
     1801 cccacggaat ttgaaatcaa aggaggccat gcaagatcaa agaactggag gctgagtgtg
     1861 cgctgtggcg ggtggcccct acgatggctg atggagaatg gatttctgcc tgatcctcca
     1921 agaatacgtt acaggaaaaa aaagagaata ctgaagtctc aaaacaatag ctcagttgac
     1981 ccttgtatga gaaacctgga tgagtgtgag gtgtgccggg acggagggga gctgttctgt
     2041 tgcgacactt gttcaagagt cttccatgag gactgtcaca tcccgcctgt ggaagctgag
     2101 aggaccccgt ggaattgcat cttctgcagg atgaaggagt ctccgggaag ccaacagtgt
     2161 tgtcaggaat ctgaggtcct ggagaggcag atgtgtcctg aggaacagtt gaaatgtgag
     2221 ttcctcctct tgaaagtcta ttgctgttct gagagctcct tttttgccaa gattccatac
     2281 tattattata ttagagaggc gtgtcaaggc ctgaaggagc ccatgtggtt ggataaaatc
     2341 aagaaaaggc tgaatgagca cggttacccc caagtggagg ggtttgtaca agacatgcgc
     2401 ctcatcttcc agaaccacag ggcctcttac aagtacaagg attttggcca aatgggattt
     2461 agactggagg ctgagtttga gaagaatttc aaggaagtgt ttgctattca ggaaacaaat
     2521 gggaacaatt agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     2581 atatcttgtg gaaaggacga gcgtgctcag ccgctactaa acgt
//
