LOCUS       sequence-285198-        7646 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7646)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 7646)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0702
FEATURES             Location/Qualifiers
     source          1..7646
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 accgccacca tgtcgggatc cacacagcct gtggcacaga cgtggagggc cactgagccc
       61 cgctacccgc cccacagcct ttcctaccca gtgcagatcg cccggacgca cacggacgtc
      121 gggctcctgg agtaccagca ccactcccgc gactatgcct cccacctgtc gcccggctcc
      181 atcatccagc cccagcggcg gaggccctcc ctgctgtctg agttccagcc cgggaatgaa
      241 cggtcccagg agctccacct gcggccagag tcccactcat acctgcccga gctggggaag
      301 tcagagatgg agttcattga aagcaagcgc cctcggctag agctgctgcc tgaccccctg
      361 ctgcgaccgt cacccctgct ggccacgggc cagcctgcgg gatctgaaga cctcaccaag
      421 gaccgtagcc tgacgggcaa gctggaaccg gtgtctcccc ccagcccccc gcacactgac
      481 cctgagctgg agctggtgcc gccacggctg tccaaggagg agctgatcca gaacatggac
      541 cgcgtggacc gagagatcac catggtagag cagcagatct ctaagctgaa gaagaagcag
      601 caacagctgg aggaggaggc tgccaagccg cccgagcctg agaagcccgt gtcaccgccg
      661 cccatcgagt cgaagcaccg cagcctggtg cagatcatct acgacgagaa ccggaagaag
      721 gctgaagctg cacatcggat tctggaaggc ctggggcccc aggtggagct gccgctgtac
      781 aaccagccct ccgacacccg gcagtatcat gagaacatca aaataaacca ggcgatgcgg
      841 aagaagctaa tcttgtactt caagaggagg aatcacgctc ggaaacaatg ggagcagaag
      901 ttctgccagc gctatgacca gctcatggag gcctgggaga agaaggtgga gcgcatcgag
      961 aacaaccccc ggcggcgggc caaggagagc aaggtgcgcg agtactacga gaagcagttc
     1021 cctgagatcc gcaagcagcg cgagctgcag gagcgcatgc agagcagggt gggccagcgg
     1081 ggcagtgggc tgtccatgtc ggccgcccgc agcgagcacg aggtgtcaga gatcatcgat
     1141 ggcctctcag agcaggagaa cctggagaag cagatgcgcc agctggccgt gatcccgccc
     1201 atgctgtacg acgctgacca gcagcgcatc aagttcatca acatgaacgg gcttatggcc
     1261 gaccccatga aggtgtacaa agaccgccag gtcatgaaca tgtggagtga gcaggagaag
     1321 gagaccttcc gggagaagtt catgcagcat cccaagaact ttggcctgat cgcatcattc
     1381 ctggagagga agacagtggc tgagtgcgtc ctctattact acctgactaa gaagaatgag
     1441 aactataaga gcctggtgag acggagctat cggcgccgcg gcaagagcca gcagcagcaa
     1501 caacagcagc agcagcagca gcagcagcag cagcagcagc ccatgccccg cagcagccag
     1561 gaggagaaag atgagaagga gaaggaaaag gaggcggaga aggaggagga gaagccggag
     1621 gtggagaacg acaaggaaga cctcctcaag gagaagacag acgacacctc aggggaggac
     1681 aacgacgaga aggaggctgt ggcctccaaa ggccgcaaaa ctgccaacag ccagggaaga
     1741 cgcaaaggcc gcatcacccg ctcaatggct aatgaggcca acagcgagga ggccatcacc
     1801 ccccagcaga gcgccgagct ggcctccatg gagctgaatg agagttctcg ctggacagaa
     1861 gaagaaatgg aaacagccaa gaaaggtctc ctggaacacg gccgcaactg gtcggccatc
     1921 gcccggatgg tgggctccaa gactgtgtcg cagtgtaaga acttctactt caactacaag
     1981 aagaggcaga acctcgatga gatcttgcag cagcacaagc tgaagatgga gaaggagagg
     2041 aacgcgcgga ggaagaagaa gaaagcgccg gcggcggcca gcgaggaggc tgcattcccg
     2101 cccgtggtgg aggatgagga gatggaggcg tcgggcgtga gcggaaatga ggaggagatg
     2161 gtggaggagg ctgaagcctt acatgcctct gggaatgagg tgcccagagg ggaatgcagt
     2221 ggcccagcca ctgtcaacaa cagctcagac accgagagca tcccctctcc tcacactgag
     2281 gccgccaagg acacagggca gaatgggccc aagcccccag ccaccctggg cgccgacggg
     2341 ccacccccag ggccacccac cccaccaccg gaggacatcc cggcccccac tgagcccacc
     2401 ccggcctctg aagccaccgg agcccctacg cccccaccag cacccccatc gccctctgca
     2461 cctcctcctg tggtccccaa ggaggagaag gaggaggaga ccgcagcagc gcccccagtg
     2521 gaggaggggg aggagcagaa gccccccgcg gctgaggagc tggcagtgga cacagggaag
     2581 gccgaggagc ccgtcaagag cgagtgcacg gaggaagccg aggaggggcc ggccaagggc
     2641 aaggacgcgg aggccgctga ggccacggcc gagggggcgc tcaaggcaga gaagaaggag
     2701 ggcgggagcg gcagggccac cacagccaag agctcgggcg ccccccagga cagcgactcc
     2761 agtgctacct gcagtgcaga cgaggtggat gaggccgagg gcggcgacaa gaaccggctg
     2821 ctgtccccaa ggcccagcct cctcaccccg actggcgacc cccgggccaa tgcctcaccc
     2881 cagaagccac tggacctgaa gcagctgaag cagcgagcgg ctgccatccc ccccatccag
     2941 gtcaccaaag tccatgagcc cccccgggag gacgcagctc ccaccaagcc agctccccca
     3001 gccccaccgc caccgcaaaa cctgcagccg gagagcgacg cccctcagca gcctggcagc
     3061 agcccccggg gcaagagcag gagcccggca ccccccgccg acaaggaggc cttcgcagcc
     3121 gaggcccaga agctgcctgg ggacccccct tgctggactt ccggcctgcc cttccccgtg
     3181 cccccccgtg aggtgatcaa ggcctccccg catgccccgg acccctcagc cttctcctac
     3241 gctccacctg gtcacccact gcccctgggc ctccatgaca ctgcccggcc cgtcctgccg
     3301 cgcccaccca ccatctccaa cccgcctccc ctcatctcct ctgccaagca ccccagcgtc
     3361 ctcgagaggc aaataggtgc catctcccaa ggaatgtcgg tccagctcca cgtcccgtac
     3421 tcagagcatg ccaaggcccc ggtgggccct gtcaccatgg ggctgcccct gcccatggac
     3481 cccaaaaagc tggcaccctt cagcggagtg aagcaggagc agctgtcccc acggggccag
     3541 gctgggccac cggagagcct gggggtgccc acagcccagg aggcgtccgt gctgagaggg
     3601 acagctctgg gctcagttcc gggcggaagc atcaccaaag gcattcccag cacacgggtg
     3661 ccctcggaca gcgccatcac ataccgcggc tccatcaccc acggcacgcc agctgacgtc
     3721 ctgtacaagg gcaccatcac caggatcatc ggcgaggaca gcccgagtcg cttggaccgc
     3781 ggccgggagg acagcctgcc caagggccac gtcatctacg aaggcaagaa gggccacgtc
     3841 ttgtcctatg agggtggcat gtctgtgacc cagtgctcca aggaggacgg cagaagcagc
     3901 tcaggacccc cccatgagac ggccgccccc aagcgcacct atgacatgat ggagggccgc
     3961 gtgggcagag ccatctcctc agccagcatc gaaggtctca tgggccgtgc catcccgccg
     4021 gagcgacaca gcccccacca cctcaaagag cagcaccaca tccgcgggtc catcacacaa
     4081 gggatccctc ggtcctacgt ggaggcacag gaggactacc tgcgtcggga ggccaagctc
     4141 ctaaagcggg agggcacgcc tccgccccca ccgccctcac gggacctgac cgaggcctac
     4201 aagacgcagg ccctgggccc cctgaagctg aagccggccc atgagggcct ggtggccacg
     4261 gtgaaggagg cgggccgctc catccatgag atcccgcgcg aggagctgcg gcacacgccc
     4321 gagctgcccc tggccccgcg gccgctcaag gagggctcca tcacgcaggg caccccgctc
     4381 aagtacgaca ccggcgcgtc caccactggc tccaaaaagc acgacgtacg ctccctcatc
     4441 ggcagccccg gccggacgtt cccacccgtg cacccgctgg atgtgatggc cgacgcccgg
     4501 gcactggaac gtgcctgcta cgaggagagc ctgaagagcc ggccagggac cgccagcagc
     4561 tcggggggct ccattgcgcg cggcgccccg gtcattgtgc ctgagctggg taagccgcgg
     4621 cagagccccc tgacctatga ggaccacggg gcaccctttg ccggccacct cccacgaggt
     4681 tcgcccgtga ccacgcggga gcccacgccg cgcctgcagg agggcagcct ttcgtccagc
     4741 aaggcatccc aggaccgaaa gctgacgtcg acgcctcgtg agatcgccaa gtccccgcac
     4801 agcaccgtgc ccgagcacca cccacacccc atctcgccct atgagcacct gcttcggggc
     4861 gtgagtggcg tggacctgta tcgcagccac atccccctgg ccttcgaccc cacctccata
     4921 ccccgcggca tccctctgga cgcagccgct gcctactacc tgccccgaca cctggccccc
     4981 aaccccacct acccgcacct gtacccaccc tacctcatcc gcggctaccc cgacacggcg
     5041 gcgctggaga accggcagac catcatcaat gactacatca cctcgcagca gatgcaccac
     5101 aacgcggcca ccgccatggc ccagcgagct gatatgctga ggggcctctc gccccgcgag
     5161 tcctcgctgg cactcaacta cgctgcgggt ccccgaggca tcatcgacct gtcccaagtg
     5221 ccacacctgc ctgtgctcgt gcccccgaca ccaggcaccc cagccaccgc catggaccgc
     5281 cttgcctacc tccccaccgc gccccagccc ttcagcagcc gccacagcag ctccccactc
     5341 tccccaggag gtccaacaca cttgacaaaa ccaaccacca cgtcctcgtc cgagcgggag
     5401 cgagaccggg atcgagagcg ggaccgggat cgggagcggg aaaagtccat cctcacgtcc
     5461 accacgacgg tggagcacgc acccatctgg agacctggta cagagcagag cagcggcagc
     5521 agcggcgggg gtgggggcag cagcagccgc cccgcctccc actcccatgc ccaccagcac
     5581 tcgcccatct cccctcggac ccaggatgcc ctccagcaga gacccagtgt gcttcacaac
     5641 acaggcatga agggtatcat caccgctgtg gagcccagca cgcccacggt cctgaggtcc
     5701 acctccacct cctcacccgt tcgcccggct gccacattcc cacctgccac ccactgccca
     5761 ctgggcggca ccctcgatgg ggtctaccct accctcatgg agcccgtctt gctgcccaag
     5821 gaggcccccc gggtcgcccg gccagagcgg ccccgagcag acaccggcca tgccttcctc
     5881 gccaagcccc cagcccgctc cgggctggag cccgcctcct cccccagcaa gggctcggag
     5941 ccccggcccc tagtgcctcc tgtctctggc cacgccacca tcgcccgcac ccctgcgaag
     6001 aacctcgcac ctcaccacgc cagcccggac ccgccggcgc cacctgcctc ggcctcggac
     6061 ccgcaccggg aaaagactca aagtaaaccc ttttccatcc aggaactgga actccgttct
     6121 ctgggttacc acggcagcag ctacagcccc gaaggggtgg agcccgtcag ccctgtgagc
     6181 tcacccagtc tgacccacga caaggggctc cccaagcacc tggaagagct cgacaagagc
     6241 cacctggagg gggagctgcg gcccaagcag ccaggccccg tgaagcttgg cggggaggcc
     6301 gcccacctcc cacacctgcg gccgctgcct gagagccagc cctcgtccag cccgctgctc
     6361 cagaccgccc caggggtcaa aggtcaccag cgggtggtca ccctggccca gcacatcagt
     6421 gaggtcatca cacaggacta cacccggcac cacccacagc agctcagcgc acccctgccc
     6481 gcccccctct actccttccc tggggccagc tgccccgtcc tggacctccg ccgcccaccc
     6541 agtgacctct acctcccgcc cccggaccat ggtgccccgg cccgtggctc cccccacagc
     6601 gaagggggca agaggtctcc agagccaaac aagacgtcgg tcttgggtgg tggtgaggac
     6661 ggtattgaac ctgtgtcccc accggagggc atgacggagc cagggcactc ccggagtgct
     6721 gtgtacccgc tgctgtaccg ggatggggaa cagacggagc ccagcaggat gggctccaag
     6781 tctccaggca acaccagcca gccgccagcc ttcttcagca agctgaccga gagcaactcc
     6841 gccatggtca agtccaagaa gcaagagatc aacaagaagc tgaacaccca caaccggaat
     6901 gagcctgaat acaatatcag ccagcctggg acggagatct tcaatatgcc cgccatcacc
     6961 ggaacaggcc ttatgaccta tagaagccag gcggtgcagg aacatgccag caccaacatg
     7021 gggctggagg ccataattag aaaggcactc atgggtaaat atgaccagtg ggaagagtcc
     7081 ccgccgctca gcgccaatgc ttttaaccct ctgaatgcca gtgccagcct gcccgctgct
     7141 atgcccataa ccgctgctga cggacggagt gaccacacac tcacctcgcc aggtggcggc
     7201 gggaaggcca aggtctctgg cagacccagc agccgaaaag ccaagtcccc ggccccgggc
     7261 ctggcatctg gggaccggcc accctctgtc tcctcagtgc actcggaggg agactgcaac
     7321 cgccggacgc cgctcaccaa ccgcgtgtgg gaggacaggc cctcgtccgc aggttccacg
     7381 ccattcccct acaaccccct gatcatgcgg ctgcaggcgg gtgtcatggc ttccccaccc
     7441 ccaccgggcc tccccgcggg cagcgggccc ctcgctggcc cccaccacgc ctgggacgag
     7501 gagcccaagc cactgctctg ctcgcagtac gagacactct ccgacagcga gtagtaatga
     7561 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     7621 gaccagctag ccagataata cagaag
//
