LOCUS       sequence-285204-        2234 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1013
FEATURES             Location/Qualifiers
     source          1..2234
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    574..585
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     protein_bind    592..603
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     CDS             1651..1662
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 accgccacca tggtgaagct cgcgaaggca ggtaaaaatc aaggtgaccc caagaaaatg
       61 gctcctcctc caaaggaggt agaagaagat agtgaagatg aggaaatgtc agaagatgaa
      121 gaagatgata gcagtggaga agaggtcgtc atacctcaga agaaaggcaa gaaggctgct
      181 gcaacctcag caaagaaggt ggtcgtttcc ccaacaaaaa aggttgcagt tgccacacca
      241 gccaagaaag cagctgtcac tccaggcaaa aaggcagcag caacacctgc caagaagaca
      301 gttacaccag ccaaagcagt taccacacct ggcaagaagg gagccacacc aggcaaagca
      361 ttggtagcaa ctcctggtaa gaagggtgct gccatcccag ccaagggggc aaagaatggc
      421 aagaatgcca agaaggaaga cagtgatgaa gaggaggatg atgacagtga ggaggatgag
      481 gaggatgacg aggacgagga tgaggatgaa gatgaaattg aaccagcagc gatgaaagca
      541 gcagctgctg cccctgcctc agaggatgag gacgatgagg atgacgaaga tgatgaggat
      601 gacgatgacg atgaggaaga tgactctgaa gaagaagcta tggagactac accagccaaa
      661 ggaaagaaag ctgcaaaagt tgttcctgtg aaagccaaga acgtggctga ggatgaagat
      721 gaagaagagg atgatgagga cgaggatgac gacgacgacg aagatgatga agatgatgat
      781 gatgaagatg atgaggagga ggaagaagag gaggaggaag agcctgtcaa agaagcacct
      841 ggaaaacgaa agaaggaaat ggccaaacag aaagcagctc ctgaagccaa gaaacagaaa
      901 gtggaaggca cagaaccgac tacggctttc aatctctttg ttggaaacct aaactttaac
      961 aaatctgctc ctgaattaaa aactggtatc agcgatgttt ttgctaaaaa tgatcttgct
     1021 gttgtggatg tcagaattgg tatgactagg aaatttggtt atgtggattt tgaatctgct
     1081 gaagacctgg agaaagcgtt ggaactcact ggtttgaaag tctttggcaa tgaaattaaa
     1141 ctagagaaac caaaaggaaa agacagtaag aaagagcgag atgcgagaac acttttggct
     1201 aaaaatctcc cttacaaagt cactcaggat gaattgaaag aagtgtttga agatgctgcg
     1261 gagatcagat tagtcagcaa ggatgggaaa agtaaaggga ttgcttatat tgaatttaag
     1321 acagaagctg atgcagagaa aacctttgaa gaaaagcagg gaacagagat cgatgggcga
     1381 tctatttccc tgtactatac tggagagaaa ggtcaaaatc aagactatag aggtggaaag
     1441 aatagcactt ggagtggtga atcaaaaact ctggttttaa gcaacctctc ctacagtgca
     1501 acagaagaaa ctcttcagga agtatttgag aaagcaactt ttatcaaagt accccagaac
     1561 caaaatggca aatctaaagg gtatgcattt atagagtttg cttcattcga agacgctaaa
     1621 gaagctttaa attcctgtaa taaaagggaa attgagggca gagcaatcag gctggagttg
     1681 caaggaccca ggggatcacc taatgccaga agccagccat ccaaaactct gtttgtcaaa
     1741 ggcctgtctg aggataccac tgaagagaca ttaaaggagt catttgacgg ctccgttcgg
     1801 gcaaggatag ttactgaccg ggaaactggg tcctccaaag ggtttggttt tgtagacttc
     1861 aacagtgagg aggatgccaa agctgccaag gaggccatgg aagacggtga aattgatgga
     1921 aataaagtta ccttggactg ggccaaacct aagggtgaag gtggcttcgg gggtcgtggt
     1981 ggaggcagag gcggctttgg aggacgaggt ggtggtagag gaggccgagg aggatttggt
     2041 ggcagaggcc ggggaggctt tggagggcga ggaggcttcc gaggaggcag aggaggagga
     2101 ggtgaccaca agccacaagg aaagaagacg aagtttgaat agtaatgaac tagtccgtaa
     2161 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga gtagtgtttg
     2221 tcttaactta tgcc
//
