LOCUS       sequence-285210-        2396 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1510
FEATURES             Location/Qualifiers
     source          1..2396
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcgggctg gatccaggcc cagcagctgc agggagacgc gctgcgccag
       61 atgcaggtgc tgtacggcca gcacttcccc atcgaggtcc ggcactactt ggcccagtgg
      121 attgagagcc agccatggga tgccattgac ttggacaatc cccaggacag agcccaagcc
      181 acccagctcc tggagggcct ggtgcaggag ctgcagaaga aggcggagca ccaggtgggg
      241 gaagatgggt ttttactgaa gatcaagctg gggcactacg ccacgcagct ccagtgcagc
      301 tctccggctg ggatcctggt tgacgccatg tcccagaagc accttcagat caaccagaca
      361 tttgaggagc tgcgactggt cacgcaggac acagagaatg agctgaagaa actgcagcag
      421 actcaggagt acttcatcat ccagtaccag gagagcctga ggatccaagc tcagtttgcc
      481 cagctggccc agctgagccc ccaggagcgt ctgagccggg agacggccct ccagcagaag
      541 caggtgtctc tggaggcctg gttgcagcgt gaggcacaga cactgcagca gtaccgcgtg
      601 gagctggccg agaagcacca gaagaccctg cagctgctgc ggaagcagca gaccatcatc
      661 ctggatgacg agctgatcca gtggaagcgg cggcagcagc tggccgggaa cggcgggccc
      721 cccgagggca gcctggacgt gctacagtcc tggtgtgaga agttggccga gatcatctgg
      781 cagaaccggc agcagatccg cagggctgag cacctctgcc agcagctgcc catccccggc
      841 ccagtggagg agatgctggc cgaggtcaac gccaccatca cggacattat ctcagccctg
      901 gtgaccagca cattcatcat tgagaagcag cctcctcagg tcctgaagac ccagaccaag
      961 tttgcagcca ccgtacgcct gctggtgggc gggaagctga acgtgcacat gaatcccccc
     1021 caggtgaagg ccaccatcat cagtgagcag caggccaagt ctctgcttaa aaatgagaac
     1081 acccgcaacg agtgcagtgg tgagatcctg aacaactgct gcgtgatgga gtaccaccaa
     1141 gccacgggca ccctcagtgc ccacttcagg aacatgtcac tgaagaggat caagcgtgct
     1201 gaccggcggg gtgcagagtc cgtgacagag gagaagttca cagtcctgtt tgagtctcag
     1261 ttcagtgttg gcagcaatga gcttgtgttc caggtgaaga ctctgtccct acctgtggtt
     1321 gtcatcgtcc acggcagcca ggaccacaat gccacggcta ctgtgctgtg ggacaatgcc
     1381 tttgctgagc cgggcagggt gccatttgcc gtgcctgaca aagtgctgtg gccgcagctg
     1441 tgtgaggcgc tcaacatgaa attcaaggcc gaagtgcaga gcaaccgggg cctgaccaag
     1501 gagaacctcg tgttcctggc gcagaaactg ttcaacaaca gcagcagcca cctggaggac
     1561 tacagtggcc tgtccgtgtc ctggtcccag ttcaacaggg agaacttgcc gggctggaac
     1621 tacaccttct ggcagtggtt tgacggggtg atggaggtgt tgaagaagca ccacaagccc
     1681 cactggaatg atggggccat cctaggtttt gtgaataagc aacaggccca cgacctgctc
     1741 atcaacaagc ccgacgggac cttcttgttg cgctttagtg actcagaaat cgggggcatc
     1801 accatcgcct ggaagtttga ctccccggaa cgcaacctgt ggaacctgaa accattcacc
     1861 acgcgggatt tctccatcag gtccctggct gaccggctgg gggacctgag ctatctcatc
     1921 tatgtgtttc ctgaccgccc caaggatgag gtcttctcca agtactacac tcctgtgctg
     1981 gctaaagctg ttgatggata tgtgaaacca cagatcaagc aagtggtccc tgagtttgtg
     2041 aatgcatctg cagatgctgg gggcagcagc gccacgtaca tggaccaggc cccctcccca
     2101 gctgtgtgcc cccaggctcc ctataacatg tacccacaga accctgacca tgtactcgat
     2161 caggatggag aattcgacct ggatgagacc atggatgtgg ccaggcacgt ggaggaactc
     2221 ttacgccgac caatggacag tcttgactcc cgcctctcgc cccctgccgg tcttttcacc
     2281 tctgccagag gctccctctc atagtaatga actagtccgt aacttgaaag tatttcgatt
     2341 tcttggcttt atatatcttg tggaaaggac gagagggaca ccctagtaga gaaacg
//
