LOCUS       sequence-285219-        6500 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6500)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 6500)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1607
FEATURES             Location/Qualifiers
     source          1..6500
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1951..1965
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
ORIGIN
        1 accgccacca tggagtctgg agaacggtta ccatcctcag cagcctcctc tactacacca
       61 acttcatctt cgacaccttc tgtggcttca gtagtttcaa aaggtggcct ttccactgga
      121 gttgcttcac ttagctctac aatcaaccca tgtggacatt tattcagaac agctggggat
      181 caaccgttta acctgtccac agtgtcgagt gccttcccaa tggtcagcca cccagtcttt
      241 ggtctacatt cagccagctc agggcattca gaatttggtg gtttggggac acttggtaca
      301 cccacagcct tagccgcaca tccccaacta gcatcttttc cagaatggtg gcgaacaact
      361 gatgctcata ctcgtacagg agcaaccttc tttccaccat tactgggaat tccaccacta
      421 tttgctcccc cagcccagaa tcatgattct tcttcattcc attcaaggac ttcgggaaaa
      481 agtaatcgaa atggtcccga aaaaggtgta aatgggtcaa taaatggaag taatacatca
      541 tctgtaattg gtatcaacac atctgtacta tccactactg cttcaagttc catgggacaa
      601 actaaaagta caagctcagg tggaggaaat cgaaaatgta atcaggaaca aagcaaaaac
      661 cagcctttgg atgctagagt tgacaaaatc aaagataaga aaccaaggaa gaaagcaatg
      721 gaaagttcta gcaacagtga tagtgattca ggcacatcat cagacacctc aagtgaaggc
      781 attagtagca gtgattcaga tgatctagaa gaagatgaag aagaagaaga tcaaagtatt
      841 gaagaaagtg aagatgatga ttctgattca gagagtgaag cacaacataa aagtaacaac
      901 caggtgctat tacatggtat ttcagaccca aaagcagatg gacagaaagc aactgaaaaa
      961 gcccaggaaa aaagaataca ccagccatta cctcttgcgt ctgaatccca gactcactca
     1021 ttccaatccc agcagaagca gcctcaggtt ttgtcacagc agcttccatt tattttccaa
     1081 agctctcagg caaaggagga atctgtgaac aaacacacca gtgtaataca gtctacggga
     1141 ttggtgtcca atgtgaaacc tttatctttg gtaaatcaag ccaaaaagga aacttacatg
     1201 aaactcatag ttccttctcc tgatgttctt aaagcaggga ataaaaatac ctctgaagaa
     1261 tctagtttat tgaccagtga attgagatcc aaacgggaac aatataaaca ggcattccca
     1321 tcacagttaa agaaacaaga gtcatcgaag agcctgaaga aggttattgc agctttgtca
     1381 aatccaaaag caacctctag ttcaccagca catccaaaac aaacattaga aaacaaccac
     1441 ccaaatccat tcttgacaaa tgcactttta ggtaatcacc aaccaaatgg agttattcaa
     1501 agtgtcattc aagaagctcc tctagcactt actaccaaaa ctaaaatgca gagcaagatt
     1561 aatgaaaaca ttgctgctgc aagtagcacc cctttttcct cacctgtaaa tctgagtaca
     1621 agtgggagaa gaacccctgg caatcagaca cctgtaatgc cctctgcctc tcccatcctg
     1681 catagtcaag ggaaggaaaa agcagttagc aataatgtaa acccagtaaa aacacagcat
     1741 cactcccatc ctgcaaaatc tttagtggaa caattcagag gaacagattc agacattccc
     1801 agtagtaaag attctgaaga ttcaaatgag gatgaagagg aagatgatga agaagaagat
     1861 gaggaagatg atgaagatga tgaatctgat gacagccaat cagaatcaga tagtaattca
     1921 gaatcagata cagaaggatc agaagaagaa gatgatgatg ataaagacca agatgaatca
     1981 gatagtgata ctgaaggaga gaaaacttca atgaaactga ataaaacaac ttcctctgtc
     2041 aaaagccctt ccatgagtct cacaggtcac tcaacacctc gtaacctcca catagcaaaa
     2101 gccccaggct ctgctcctgc tgccttatgt tctgaatccc agtcacctgc ttttcttggt
     2161 acatcttctt ccacacttac ttcaagccca cactctggca cttccaaaag aagaagagta
     2221 acagatgaac gtgaactgcg tattccattg gaatatggct ggcagagaga gacaagaata
     2281 agaaactttg gagggcgcct tcaaggagaa gtagcatatt atgctccatg tggaaagaaa
     2341 cttaggcagt accctgaagt aataaaggga atgcagtggt gtcttttgaa agaagaggat
     2401 gtcattcctc gtatcagggc aatggaaggt cgtagaggaa gaccaccaaa tccagataga
     2461 caacgagcaa gagaggaatc caggatgaga cgtcggaaag gtcgacctcc aaatgttggc
     2521 aatgctgaat tcctagataa cgcagatgca aagttgctaa gaaaactgca agctcaagaa
     2581 atagccaggc aagcagcaca aataaagctt ttgagaaaac ttcaaaagca ggaacaggct
     2641 cgggttgcta aagaagccaa aaaacaacaa gcaataatgg ctgctgagga gaagcggaag
     2701 caaaaagaac agataaagat tatgaaacag caggaaaaaa ttaagagaat acagcaaatc
     2761 agaatggaaa aagaacttcg agctcagcaa attctagagg ctaaaaagaa aaagaaggaa
     2821 gaagcggcaa atgccaaatt attggaggcc gagaaacgaa taaaggaaaa agaaatgaga
     2881 agacaacaag ctgttcttct gaaacatcag gaacgagagc gaaggcgaca acacatgatg
     2941 cttatgaaag ctatggaagc tcgtaaaaaa gcagaagaaa aagagcggtt gaaacaagaa
     3001 aaacgtgatg agaaaagatt aaataaagag cgtaaactag agcagcgaag attagaatta
     3061 gaaatggcaa aggaactaaa gaagcctaat gaagacatgt gcttagcaga ccaaaagcct
     3121 ttgccagagt tgcctcgtat tccaggactt gttctctctg gaagtacatt ttcagactgt
     3181 ctcatggtgg tgcagttctt acgaaacttt ggtaaagttt tgggctttga tgtgaatatt
     3241 gatgttccaa acctgagtgt tcttcaagag ggattgctaa atatagggga cagcatgggt
     3301 gaagtacaag acttgcttgt gaggctcctc tcagctgctg tatgtgatcc aggtctaata
     3361 acaggataca aggctaaaac agctcttgga gaacatttgc tgaatgttgg tgtgaatcga
     3421 gacaatgttt ccgagatttt acagatattt atggaagccc actgtggaca aactgagctt
     3481 actgaaagtc tgaagaccaa agcttttcag gctcacactc cagcacagaa agcttcagtc
     3541 ctggctttcc tgatcaatga actggcatgc agcaagagtg tggtcagtga aatcgacaag
     3601 aacattgatt atatgtcaaa cttgaggaga gataaatggg tggtagaagg taaactccgc
     3661 aagctcagaa tcattcatgc taagaaaaca ggcaaaagag acacttcagg tggcattgat
     3721 ctgggagaag agcagcatcc cttgggcaca cccactccag gacgcaagcg aagaaggaag
     3781 ggaggagaca gtgattatga cgatgatgat gacgatgaca gtgatgacca aggggatgaa
     3841 gatgatgagg atgaagaaga taaagaagac aaaaaaggaa aaaagactga tatctgtgaa
     3901 gatgaggatg aaggtgacca agcagcaagt gttgaagagc tggaaaaaca gattgaaaaa
     3961 ctgagtaaac aacagagtca gtacagaagg aagctctttg atgcgtctca ctcattgcgt
     4021 tcagtgatgt ttggccaaga tcgttacaga cgccggtact ggattcttcc ccaatgtggg
     4081 gggatttttg tagaaggcat ggagagtggt gaaggactag aagaaattgc aaaagaaaga
     4141 gaaaaactga aaaaggcaga aagtgtccag atcaaagaag aaatgtttga gacttctggg
     4201 gacagtttaa attgttcaaa tacagatcac tgtgaacaaa aggaagatct taaagaaaaa
     4261 gataacacaa atctattcct tcagaaacct ggctcttttt ccaaattaag caagcttttg
     4321 gaagtagcta agatgcctcc tgagtcagag gttatgaccc ccaaaccaaa tgctggtgca
     4381 aatgggtgca cgttgtctta tcagaacagt ggaaaacatt cactgggcag cgttcagtca
     4441 acagcaacgc aaagcaatgt ggaaaaggca gactctaata atctgtttaa tactggttca
     4501 agtggtccag ggaagttcta cagtcctctc cccaatgacc agttactaaa aacgctgact
     4561 gaaaagaata gacaatggtt tagtcttttg ccacgaacac cctgtgatga cacttcactt
     4621 actcatgccg atatgtcaac tgcttctttg gtgactcctc agtctcagcc accatctaag
     4681 tcaccttcac ctaccccagc tcctcttgga tcttctgctc agaatcctgt tggcttaaat
     4741 ccatttgctt tatcacctct tcaggtgaaa ggtggagtat ctatgatggg acttcagttt
     4801 tgtggatggc ccactggtgt ggttacttct aatattccat ttacatcatc tgtacctagt
     4861 ctaggatcgg ggttagggtt atcagaagga aatggtaatt cattcttgac ttccaatgtt
     4921 gcttcaagta aaagtgaatc tccagtacca cagaatgaaa aggccacttc agctcaacct
     4981 gcagctgttg aagtagcaaa accagtagat tttcctagtc caaaacctat tccagaagaa
     5041 atgcagtttg gttggtggag aattattgac ccagaggacc taaaagcttt gctcaaagtg
     5101 ctgcatctca gaggaataag agaaaaggca ttacaaaaac aaattcagaa acatttggat
     5161 tatattactc aagcctgcct caagaataag gatgttgcta ttattgaact gaatgaaaat
     5221 gaagaaaacc aagtaactcg agatattgtg gagaactggt cagtagaaga acaagcaatg
     5281 gaaatggatt tgagtgtcct tcaacaggta gaagatctag aaaggagagt tgcatcagca
     5341 agtttgcaag tgaagggttg gatgtgtcca gagcctgcat cagaaaggga ggacttggta
     5401 tattttgaac ataaatcatt tactaaattg tgcaaggagc atgatggaga atttactggc
     5461 gaagacgaaa gcagtgcaca tgcactagaa cggaagagtg acaaccccct agatatagct
     5521 gtaaccaggc tggctgattt ggagcggaac attgaaagaa gaattgaaga ggatattgct
     5581 ccagggctca gggtatggag aagggcatta tcagaagctc gcagtgctgc acaggtagct
     5641 ctgtgcattc agcaattaca gaaatcaata gcatgggaaa aatcaattat gaaagtttac
     5701 tgccaaatct gtcgaaaggg agataatgaa gaactgcttc ttctttgtga tggctgtgac
     5761 aaaggctgtc atacctactg ccatagaccc aagattacaa caatcccaga tggagactgg
     5821 ttttgtccag cttgcattgc taaggcaagt ggtcaaactc taaaaatcaa aaaacttcat
     5881 gtcaaaggaa aaaagactaa tgagtcaaag aaaggcaaga aggtaacttt aacaggagat
     5941 actgaagatg aagactctgc atctacaagt agttcactaa aaagaggaaa caaagacctc
     6001 aagaaaagaa aaatggagga aaacacttct attaacttgt caaaacaaga aagttttact
     6061 tcagttaaga aacctaaaag agatgactcc aaggacctag ctctttgcag tatgattctg
     6121 actgaaatgg aaactcatga ggatgcatgg ccttttctac ttcctgtaaa cttgaaactt
     6181 gttcctggtt ataagaaagt tattaagaag cctatggatt tttccacaat tagagagaaa
     6241 ctaagtagtg gacagtatcc aaaccttgaa acctttgctc tagatgtcag gcttgttttt
     6301 gacaactgtg aaacatttaa tgaagatgat tctgatatag gcagagctgg ccacaatatg
     6361 aggaagtatt ttgaaaaaaa gtggacagat actttcaaag tgagctagta atgaactagt
     6421 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaaagg
     6481 ttcgcgatcc acaggaggtc
//
