LOCUS       sequence-285222-        2579 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1634
FEATURES             Location/Qualifiers
     source          1..2579
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(1422..1433)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     RBS             1850..1858
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 accgccacca tggagacttt gacctcaagg catgagaaaa gagctcttca ttctcaagcc
       61 tcagccattt cccaagatag ggaggagaag atcatgtctc aggagccatt gagcttcaag
      121 gacgtggctg tggtcttcac tgaggaggag ctagagctgc tggactctac ccagaggcag
      181 ctgtaccaag atgtgatgca ggagaatttc aggaacctac tctcagtggg tgagaggaat
      241 cctctgggag acaagaatgg aaaggatacg gagtatattc aagatgaaga attaaggttc
      301 ttttcacaca aagagctctc ctcatgcaaa atctgggaag aggtggcagg tgaattacct
      361 gggagccaag actgtagagt aaatctgcaa ggaaaagact tccagttctc agaagatgct
      421 gctccccatc aagggtggga aggagcatct acgccgtgtt ttccaattga gaatttcctg
      481 gacagtctac aaggggatgg gcttatcggt ctagaaaatc aacagtttcc agcctggaga
      541 gctataagac caatccccat tcaaggatct tgggcaaaag cgtttgtgaa ccagttaggg
      601 gatgttcaag aaagatgtaa aaatctcgac acagaagaca cagtatataa atgtaactgg
      661 gatgatgaca gcttttgctg gatatcttgt catgttgatc acagattccc tgaaatagac
      721 aagccgtgtg gttgcaataa atgcagaaaa gactgcatta aaaactctgt acttcatcgc
      781 attaaccctg gagagaatgg cttgaaaagt aacgaataca gaaatggctt cagggacgat
      841 gcagaccttc ccccgcatcc aagagtacct ttgaaagaga aactctgtca atatgatgag
      901 tttagtgagg gcttgaggca cagtgcccat cttaacagac atcaaagagt tcccacagga
      961 gagaaatctg ttaagagtct tgagcgtggt cggggcgtca gacagaacac gcacatacgt
     1021 aaccacccca gagcccctgt gggagacatg ccctatagat gtgatgtctg tggaaagggg
     1081 ttcaggtata aatcggttct tcttattcat caaggggtgc acacaggaag gagaccctat
     1141 aaatgtgagg agtgtgggaa ggcatttggt cgaagttcaa acctgcttgt ccatcagagg
     1201 gtccacactg gagagaaacc atataaatgc agcgagtgtg ggaagggctt cagttacagc
     1261 tcagtgcttc aagtccatca gaggctgcac acaggggaga agccctacac ctgcagcgag
     1321 tgtggcaaag gcttctgtgc caagtctgca ctgcacaaac accagcacat tcaccctgga
     1381 gaaaagccct acagctgtgg cgagtgtgga aagggattca gctgcagctc ccacctcagc
     1441 agtcatcaga agacacacac cggcgagagg ccctaccagt gtgacaagtg tggcaaaggt
     1501 ttcagtcaca actcgtacct tcaagctcac cagagagttc acatggggca gcatctgtac
     1561 aaatgtaacg tgtgtggtaa gagtttcagt tacagctcag ggcttctcat gcatcagaga
     1621 ctgcacacag gagagaaacc ctacaaatgc gagtgcggga agagctttgg ccggagctcc
     1681 gacctccaca tccatcagag ggtccacaca ggagagaaac cctataaatg cagtgagtgt
     1741 gggaagggct tccggcggaa ttcagacctt cacagccacc agagggtcca cacgggagag
     1801 aggccctacg tgtgtgacgt gtgtgggaag ggtttcatct acagctccga cctccttatc
     1861 catcagaggg tccacactgg agagaaaccc tataaatgtg ctgagtgtgg caaaggcttc
     1921 agttacagct cagggcttct cattcaccag agagtccaca caggcgagaa accttacaga
     1981 tgccaagagt gcggaaaggg ctttaggtgc acatcaagcc ttcacaaaca tcagcgagtc
     2041 cacacgggaa aaaagcccta tacgtgtgat cagtgtggca agggattcag ttatggctct
     2101 aatcttcgca cccaccagag gttgcacaca ggagagaaac cctacacttg ttgtgaatgt
     2161 gggaagggtt tcagatatgg ctcaggtctc cttagtcata agagagtgca cactggcgag
     2221 aagccataca gatgccacgt gtgtgggaag ggctatagtc agagctcaca tcttcaaggt
     2281 catcagaggg tccacactgg tgagaaaccc tataaatgtg aggagtgtgg gaagggcttt
     2341 ggccgcaact cctgtcttca tgttcatcag agagtccaca ctggagagaa gccctatacg
     2401 tgtggtgtgt gtgggaaagg cttcagttat acctcaggtc tgcggaacca ccaaagagtg
     2461 catttaggcg agaaccctta taagtagtaa tgaactagtc cgtaacttga aagtatttcg
     2521 atttcttggc tttatatatc ttgtggaaag gacgaaaaca ggagcaattt catcagacg
//
