LOCUS       sequence-285228-        2018 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1688
FEATURES             Location/Qualifiers
     source          1..2018
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1480..1489
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggtttctaa actgagccag ctgcagacgg agctcctggc ggccctgctc
       61 gagtcagggc tgagcaaaga ggcactgatc caggcactgg gtgagccggg gccctacctc
      121 ctggctggag aaggccccct ggacaagggg gagtcctgcg gcggcggtcg aggggagctg
      181 gctgagctgc ccaatgggct gggggagact cggggctccg aggacgagac ggacgacgat
      241 ggggaagact tcacgccacc catcctcaaa gagctggaga acctcagccc tgaggaggcg
      301 gcccaccaga aagccgtggt ggagaccctt ctgcaggagg acccgtggcg tgtggcgaag
      361 atggtcaagt cctacctgca gcagcacaac atcccacagc gggaggtggt cgataccact
      421 ggcctcaacc agtcccacct gtcccaacac ctcaacaagg gcactcccat gaagacgcag
      481 aagcgggccg ccctgtacac ctggtacgtc cgcaagcagc gagaggtggc gcagcagttc
      541 acccatgcag ggcagggagg gctgattgaa gagcccacag gtgatgagct accaaccaag
      601 aaggggcgga ggaaccgttt caagtggggc ccagcatccc agcagatcct gttccaggcc
      661 tatgagaggc agaagaaccc tagcaaggag gagcgagaga cgctagtgga ggagtgcaat
      721 agggcggaat gcatccagag aggggtgtcc ccatcacagg cacaggggct gggctccaac
      781 ctcgtcacgg aggtgcgtgt ctacaactgg tttgccaacc ggcgcaaaga agaagccttc
      841 cggcacaagc tggccatgga cacgtacagc gggccccccc cagggccagg cccgggacct
      901 gcgctgcccg ctcacagctc ccctggcctg cctccacctg ccctctcccc cagtaaggtc
      961 cacggtgtgc gctatggaca gcctgcgacc agtgagactg cagaagtacc ctcaagcagc
     1021 ggcggtccct tagtgacagt gtctacaccc ctccaccaag tgtcccccac gggcctggag
     1081 cccagccaca gcctgctgag tacagaagcc aagctggtct cagcagctgg gggccccctc
     1141 ccccctgtca gcaccctgac agcactgcac agcttggagc agacatcccc aggcctcaac
     1201 cagcagcccc agaacctcat catggcctca cttcctgggg tcatgaccat cgggcctggt
     1261 gagcctgcct ccctgggtcc tacgttcacc aacacaggtg cctccaccct ggtcatcggc
     1321 ctggcctcca cgcaggcaca gagtgtgccg gtcatcaaca gcatgggcag cagcctgacc
     1381 accctgcagc ccgtccagtt ctcccagccg ctgcacccct cctaccagca gccgctcatg
     1441 ccacctgtgc agagccatgt gacccagagc cccttcatgg ccaccatggc tcagctgcag
     1501 agcccccacg ccctctacag ccacaagccc gaggtggccc agtacaccca cacgggcctg
     1561 ctcccgcaga ctatgctcat caccgacacc accaacctga gcgccctggc cagcctcacg
     1621 cccaccaagc aggaggctgc tctgctcccc caggtcttca cctcagacac tgaggcctcc
     1681 agtgagtccg ggcttcacac gccggcatct caggccacca ccctccacgt ccccagccag
     1741 gaccctgcca gcatccagca cctgcagccg gcccaccggc tcagcgccag ccccacagtg
     1801 tcctccagca gcctggtgct gtaccagagc tcagactcca gcaatggcca gagccacctg
     1861 ctgccatcca accacagcgt catcgagacc ttcatctcca cccagatggc ctcttcctcc
     1921 cagtagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     1981 tgtggaaagg acgagctcgc gcatggcgat gataacgt
//
