LOCUS       sequence-285231-        2669 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1692
FEATURES             Location/Qualifiers
     source          1..2669
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctgccgc ggctacgctg aggctctccg ctcagggcac agtgactttt
       61 gaagatgtgg ctgtgaactt tacctgggag gaatggaatc tccttagtga ggctcagaga
      121 tgcctgtacc gtgatgtgac gctggagaac ctggcactta tatcctccct gggttgttgg
      181 tgtggagtgg aagatgaggc ggcaccttct aagcagagta tttatataca aagagagact
      241 caggtcagga ctcctatggc aggtgtgtct cccaagaagg cccacccctg tgagatgtgt
      301 ggcccgatct tgggagacat tttgcatgtg gcagatcatc agggaacaca tcacaagcag
      361 aaactgcaca ggtgtgaggc ctgggggaat aaattgtatg acagtggaaa ctttcatcag
      421 caccagaatg agcacattgg agagaaaccc tacagaggga gtgttgagga ggcgttgttt
      481 gcaaagaggt gtaagttgca tgtgtcaggg gagtcatctg tcttcagtga gagtgggaag
      541 gactttttgc ccaggtcagg attactccag caggaggcca gtcacactgg ggagaagtca
      601 aacagcaaaa ctgagtgtgt gtctcccatt cagtgtgggg gagctcacta cagctgtgga
      661 gaatccatga aacattttag caccaaacat atactcagtc agcaccagag actgctcact
      721 agagaagagt gttatgtgtg ctgtgaatgt gggaagtcct ttagcaaata tgctagcttg
      781 agtaatcatc agagagttca cactgaaaaa aaacatgaat gtggagaatg tgggaaatcc
      841 tttagcaaat atgttagctt cagtaatcat cagagagttc acactgaaaa aaaacatgaa
      901 tgtggagaat gtgggaaatc ctttagcaaa tatgttagct tcagtaatca tcagagagtt
      961 cacactggga aaagacctta tgaatgtgga gaatgtggga aatcgtttag caaatatgct
     1021 agcttcagta atcatcagag agttcacact gaaaaaaaac attatgaatg tggagaatgt
     1081 gggaaatcct ttagcaaata tgttagcttc agtaatcatc agagagttca cactgggaaa
     1141 agaccttatg aatgtggaga atgtgggaaa tcgtttagca aatatgctag cttcagtaat
     1201 catcagagag ttcacactga caaaaaacat tatgaatgtg gagaatgtgg gaaatccttt
     1261 agtcaaaaga gcagcctcat tcaacatcag cgatttcaca ctggagaaaa accttatggg
     1321 tgtgaagaat gtgggaaatc ttttagttca gaaggacatc ttaggagcca tcaacgagtt
     1381 cacgccggag aaagaccttt caagtgtgga gaatgtgtga aatctttcag tcataagcgc
     1441 agccttgttc accatcagcg agttcacagt ggagaaagac cttatcagtg tggagaatgt
     1501 gggaaatctt tcagtcaaaa gggcaacctc gttctacacc agcgagttca cactggagca
     1561 agaccttatg agtgtggaga atgtgggaaa tcatttagtt caaaaggaca tcttaggaac
     1621 catcagcaaa ttcacactgg ggacagactt tatgagtgtg gagagtgtgg gaaatctttt
     1681 agtcataaag gcaccctcat tctacatcag cgagttcacc ctagagaaag atcttatggg
     1741 tgtggagaat gtgggaaatc ttttagttca atcgggcacc ttaggagcca tcagcgcgtt
     1801 catactggag agaggcctta tgagtgtgga gaatgtggga aatcttttag tcataagcgc
     1861 agccttgttc accatcagcg catgcacact ggagaaagac cttacaagtg tggagactgt
     1921 gggaaatctt ttaatgaaaa aggacacctt aggaatcatc agcgagttca cactacagaa
     1981 agacctttta agtgtgggga atgtgggaaa tgttttagtc acaagggtaa cctcattcta
     2041 caccagcatg gccatactgg agaaagacct tatgtatgta gggaatgtgg aaaattattt
     2101 aagaagaagt ctcacctcct tgtacaccag agaattcaca atggagaaaa gccatatgct
     2161 tgtgaagctt gtcagaaatt ttttagaaac aagtaccaac tcattgcaca tcagagagtt
     2221 cacactggag aaaggcctta tgaatgcaat gattgtggaa aatcatttac ccacagctct
     2281 acattctgtg ttcataagcg aattcacact ggagaaaagc cttatgagtg cagtgaatgt
     2341 ggaaaatctt tcgctgaaag ctccagtttc acaaaacaca aaagagttca cactggagaa
     2401 aagccttatg agtgcagtga atgtggaaaa tcttttgctg aaagctccag tctcactaaa
     2461 cacaagagag ttcacactgg agaaaagcct tataaatgtg agaaatgtgg gaaattattt
     2521 aacaagaagt ctcacctcct tgtacaccag agttcacact ggagaaaagc catatagtaa
     2581 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     2641 gacgagtcca cgagaacttt ggatacgaa
//
