LOCUS       sequence-285234-        5639 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5639)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 5639)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1784
FEATURES             Location/Qualifiers
     source          1..5639
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggcctttg tgggctgctg gagagatgtt ggctgcacca tgacccagat
       61 ggagttttaa cattgaatgc ggagaacact aattatgcct atcaagttcc aaacttccat
      121 aaatgtgaaa tctgtctact atcttttcca aaagaatccc agtttcaacg ccacatgagg
      181 gatcacgagc gaaatgacaa gccacatcga tgtgaccagt gcccccaaac atttaatgtt
      241 gaattcaacc tgacacttca taaatgcacc cacagcgggg aagatcctac ctgccctgtg
      301 tgtaacaaga aattctccag agtggctagt ctcaaagcgc atattatgct acatgaaaag
      361 gaagagaatc tcatctgttc tgagtgtggg gatgagttta ctctgcagag tcagctggcc
      421 gtgcacatgg aggagcaccg ccaggagctg gctggaaccc ggcagcatgc ctgcaaggcc
      481 tgcaagaaag agttcgagac ctcctcggag ctgaaggaac acatgaagac tcattacaaa
      541 attagggtat caagtacaag gtcttataac cggaatatcg acagaagtgg attcacgtat
      601 tcgtgtccgc actgtggaaa gacgtttcaa aagccaagcc agttaacgcg acacattagg
      661 atacacacag gtgaaaggcc gttcaaatgt agtgaatgtg gaaaggcttt taaccagaag
      721 ggggcactgc agacccacat gatcaagcac acaggtgaaa aaccccatgc ctgtgccttc
      781 tgtcctgccg ccttctctca gaaagggaat cttcagtcgc acgtgcagcg agtccactca
      841 gaggtcaaga atggtcctac ctataactgt acagaatgta gttgtgtatt taaaagttta
      901 ggcagcttaa acacgcatat cagcaagatg catatgggtg ggccacagaa ttcaacaagt
      961 tctacagaga ctgctcatgt tttaacggcc acactttttc agacgttacc tcttcaacag
     1021 acggaagccc aagccacgtc ggcctcaagc cagccgagct cccaggcggt gagcgacgtc
     1081 atccagcagc tcctggagct ctcagagccg gcgccggtgg agtcggggca gtccccgcag
     1141 cctgggcagc agctgagcat cacagtgggc atcaaccagg acattttaca gcaagcctta
     1201 gaaaacagtg ggctgtcttc aattccagct gcagcacatc ctaatgactc ctgccatgcc
     1261 aagacctctg caccacacgc tcaaaaccca gatgtttcca gcgtttcaaa tgagcagacg
     1321 gaccccacag acgcagagca agaaaaagaa caggaaagcc cggagaaact ggataaaaaa
     1381 gaaaaaaaaa tgataaagaa gaagtcaccg tttctacctg gctccatccg cgaggagaac
     1441 ggcgtgcgct ggcatgtgtg tccctactgc gccaaggagt tccgcaagcc cagcgacctg
     1501 gtccgccaca tccgcatcca cacccacgag aagcccttca agtgcccgca gtgcttccgc
     1561 gccttcgccg tgaagagcac gctgacagcg cacatcaaga cgcacaccgg catcaaggcg
     1621 ttcaagtgcc agtactgcat gaagagcttc tccacctctg gcagcctcaa ggtgcacatt
     1681 cgcctgcaca caggagttag accttttgct tgtcctcact gtgacaaaaa atttcgaacc
     1741 tcaggccata ggaagactca cattgcttcc cactttaaac atacggaatt aaggaaaatg
     1801 aggcaccagc gtaaacctgc aaaggtccgt gttggcaaga cgaatattcc agtccctgat
     1861 attcctttgc aggaaccaat cctcataact gacttaggtc tcatccagcc cattccaaaa
     1921 aaccagtttt tccaaagcta tttcaataat aattttgtca atgaagcaga tagaccatac
     1981 aagtgttttt actgtcatcg tgcatataaa aaatcttgcc accttaaaca acacatcaga
     2041 tcccatacag gtgaaaaacc ttttaaatgt tctcagtgtg gaagaggctt tgtttctgca
     2101 ggcgtgctca aagcacacat cagaacacac acaggactga aatctttcaa gtgtctgata
     2161 tgtaatgggg ctttcactac tggtggcagc ttacggcgac acatgggtat ccacaacgac
     2221 cttcgtccct atatgtgtcc ctattgccaa aaaacattta agacttcact aaattgcaaa
     2281 aagcacatga aaacccacag atatgagctt gcccagcagc tccaacagca tcagcaggca
     2341 gcctcgatag atgacagcac tgtagaccag cagagcatgc aggcctccac tcaaatgcag
     2401 gtggagatcg agagcgacga gctgccgcag acggcagagg tggtcgcagc gaaccccgag
     2461 gccatgctgg acctggagcc tcagcatgtg gtgggcacgg aggaagcagg gctgggccag
     2521 cagttggcag atcagcccct ggaagcagat gaagatgggt ttgtggctcc acaggaccct
     2581 ctgcgagggc acgtagacca gtttgaagag cagagccctg cgcaacagtc cttcgaacca
     2641 gcagggctac cccaaggttt tacagtgact gatacgtacc atcagcagcc tcagtttcca
     2701 cctgtccaac agctacagga ttccagcaca cttgagtctc aggccctctc cacaagcttc
     2761 caccagcaga gcttgctgca ggctcccagc tctgatggga tgaatgtaac aactcgcttg
     2821 attcaggagt catcccaaga ggaactggac ctgcaggcac aaggttccca gtttctggag
     2881 gacaacgagg accagagcag gcgctcttac aggtgtgact attgcaacaa aggctttaag
     2941 aagtccagcc acctgaagca gcatgtgcgg tcgcacaccg gggaaaagcc ctacaagtgc
     3001 aagctctgtg gacgcggctt tgtttcctct ggggtcctca agtcccacga gaagacacac
     3061 acaggagtga aggcgttcag ctgcagtgtg tgcaatgctt ccttcaccac caatggcagc
     3121 ctcacccggc acatggccac acatatgagc atgaagcctt ataagtgtcc gttttgtgag
     3181 gagggtttcc gaactacagt gcattgtaaa aagcacatga agagacacca aacagtcccc
     3241 tctgctgtgt cagccactgg agagacagaa ggaggagaca tttgtatgga ggaagaggaa
     3301 gaacattctg acagaaatgc atcacggaag tctcgtcctg aggtcatcac tttcacggag
     3361 gaggagacag cccagttagc caagatccgg ccgcaggaga gcgccacggt gtcagagaag
     3421 gtcctggtgc agtccgcggc agaaaaggac cgcatcagtg agctgaggga caagcaggcg
     3481 gagctgcagg acgagcccaa gcacgccaac tgctgcacat actgccccaa gagcttcaag
     3541 aaacctagcg acctggtgag gcatgttcga atccatactg gagaaaagcc atacaaatgt
     3601 gatgaatgtg gaaagagttt tactgtgaaa tccactctcg attgtcatgt gaagactcac
     3661 acaggtcaga agctcttcag ctgtcacgtc tgcagcaacg ccttctccac gaagggaagt
     3721 ctgaaggtcc acatgcgcct gcacacggga gccaagccct tcaaatgccc gcattgcgag
     3781 ctgcgtttcc gtacctcggg tagaagaaag acacacatgc agtttcatta taaaccagac
     3841 ccaaagaagg ccagaaagcc tatgactcga agctcatcgg aaggactgca gcctgtaaac
     3901 ctcctcaact cctcctctac tgacccaaac gtgtttatca tgaacaactc tgttctaaca
     3961 ggacagtttg atcagaatct gctgcaacca ggactggtgg gccaagctat tctccctgcc
     4021 tctgtgtcag ctgggggtga cctgaccgtg tctctgacag atgggagcct ggctacccta
     4081 gaaggcatcc agttacagtt ggctgctaac ttggttggac caaatgtaca gatttctgga
     4141 atcgatgctg ccagcattaa taacattacg ttgcagattg atccaagcat tctgcagcag
     4201 acgctacagc agggcaacct attggctcag cagctcacgg gggagcctgg cctggcccca
     4261 cagaacagct ctctccagac atcggacagc acggtccctg ccagtgttgt catccagccc
     4321 atctcaggcc tgtccttaca gcccacagtg acctctgcga acctgaccat aggcccgctg
     4381 tctgagcagg attcagtgct gaccactaac agcagtggga cccaagacct cactcaagtg
     4441 atgacttcgc aaggtctagt gtccccctcc ggcggtcccc acgagatcac cctgaccatt
     4501 aacaactcca gcctgagcca ggtcctggca caggccgctg ggcccactgc cacgtcttcc
     4561 tcggggtctc cacaggaaat taccctgact atctccgaac ttaacactac aagcggaagc
     4621 cttccttcaa caacaccgat gtctccatcg gccatctcga ctcagaacct ggtcatgtcc
     4681 tcgtcgggcg tgggaggtga cgctagtgtc acgctgacgc tggccgatac tcagggtatg
     4741 ctatctggag gcctggacac tgtcacactc aacatcacct ctcagggtca gcagttccca
     4801 gcgctcctca cggatccctc tctctcgggc cagggtggag caggctcgcc gcaagtcata
     4861 ctagtgagcc acacgccaca gtcagcgtct gctgcttgtg aagaaatagc ctaccaggta
     4921 gctggcgtct ctgggaacct ggccccgggc aaccagccag agaaggaggg ccgggcgcac
     4981 cagtgcctgg agtgtgaccg cgccttctca tcggcggcgg tgctcatgca ccacagcaag
     5041 gaggtgcatg gccgggagcg catccacggc tgccccgtgt gcaggaaggc cttcaagcgc
     5101 gccacgcacc tcaaggagca catgcagaca caccaggccg gcccctcttt gagctcccag
     5161 aagccaagag tgtttaaatg tgacacttgt gagaaggcat ttgccaaacc aagccagctg
     5221 gagcgccaca gccgcataca tacaggggag cggccgttcc attgcacgct ttgtgagaaa
     5281 gccttcaacc agaagagtgc gctgcaggtg cacatgaaga agcacacggg ggagcggccc
     5341 tacaagtgtg cctactgcgt catgggcttc acgcagaaga gcaacatgaa gctgcacatg
     5401 aagcgggcgc acagctatgc tggagctctg caggagtctg caggtcaccc ggagcaggac
     5461 ggggaggagc tgagccggac cctccacctg gaggaggtgg tgcaggaggc cgccggcgag
     5521 tggcaggccc tcacccacgt cttctagtaa tgaactagtc cgtaacttga aagtatttcg
     5581 atttcttggc tttatatatc ttgtggaaag gacgacatgt ggccatgagg atttgcgat
//
