LOCUS       sequence-285236-       17128 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17128)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 17128)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1872
FEATURES             Location/Qualifiers
     source          1..17128
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      12659..12668
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    12985..13573
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(13038..13058)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(13456..13467)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             13645..13878
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    13956..14077
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13993..14012)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     14047..14066
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      14117..14252
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(14272..14291)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(14273..14291)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(14301..14318)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(14301..14317)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14310..14332)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      14459..14914
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(14546..14565)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     14756..14777
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        14940..15044
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(15045..15113,15114..15905)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(15263..15282)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      16076..16664
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     16565..16584
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     16818..16835
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    16952..16973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(16988..16993,16994..17011,17012..17018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    17026..17042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     17031..17053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     17050..17066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     17050..17066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     17085..17105
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        17087..17105
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgagg ggccggcggg gcaggccgcc
     4141 caagcagccc gcggctcccg ctgcggagcg ctgcgccccg gccccgccgc caccgccgcc
     4201 gccgcccacg tccggaccca tcggggggct ccgctcgcgg caccgcggca gcagccgggg
     4261 caggtgggcc gccgcccagg ctgaggtggc gcccaagacg cggctgagct cgcccagggg
     4321 gggcagcagt agccggagga agccgccgcc gccgccgccg gcccccccca gcaccagcgc
     4381 cccgggccgg ggggggcgag gaggcggggg cggcaggacg gggggcgggg gcggcggcgg
     4441 ccacctggcc cggaccaccg cggcccggag ggccgtcaac aaagtggtgt acgatgacca
     4501 cgagagcgag gaggaggagg aagaggagga catggtctcc gaggaggagg aggaggagga
     4561 cggcgacgcc gaggagaccc aggattctga ggacgacgag gaggatgaga tggaagagga
     4621 cgacgatgac tccgattatc cggaggagat ggaagacgac gacgacgacg ccagttactg
     4681 cacggaaagc agcttcagga gccatagtac ctacagcagc actccaggta ggcgaaaacc
     4741 aagagtacat cggcctcgtt ctcctatatt ggaagaaaaa gacatcccgc cccttgaatt
     4801 tcccaagtcc tctgaggatt taatggtgcc taatgagcat ataatgaatg tcattgccat
     4861 ttacgaggta ctgcggaact ttggcactgt tttgagatta tctccttttc gctttgagga
     4921 cttttgtgca gctctggtga gccaagagca gtgcacactc atggcagaga tgcatgttgt
     4981 gcttttgaaa gcagttctgc gtgaagaaga cacttccaat actacctttg gacctgctga
     5041 tctgaaagat agcgttaatt ccacactgta tttcatagat gggatgacgt ggccagaggt
     5101 gctgcgggtg tactgtgaga gtgataagga gtaccatcac gttcttcctt accaagaggc
     5161 agaggactac ccatatggac cagtagagaa caagatcaaa gttctacagt ttctagtcga
     5221 tcagtttctt acaacaaata ttgctcgaga ggaattgatg tctgaagggg tgatacagta
     5281 tgatgaccat tgtagggttt gtcacaaact tggggatttg ctttgctgtg agacatgttc
     5341 agcagtatac catttggaat gtgtgaagcc acctcttgag gaggtgccag aggacgagtg
     5401 gcagtgtgaa gtctgtgtag cacacaaggt gcctggtgtg actgactgtg ttgctgaaat
     5461 ccaaaaaaat aaaccatata ttcgacatga acctattgga tatgatagaa gtcggaggaa
     5521 atactggttc ttgaaccgaa gactcataat agaagaagat acagaaaatg aaaatgaaaa
     5581 gaaaatttgg tattacagca caaaggtcca acttgcagaa ttaattgact gtctagacaa
     5641 agattattgg gaagcagaac tctgcaaaat tctagaagaa atgcgtgaag aaatccaccg
     5701 acacatggac ataactgaag acctgaccaa taaggctcgg ggcagtaaca aatcctttct
     5761 ggcggcagct aatgaagaaa ttttggaatc cataagagcc aaaaagggag acattgataa
     5821 tgttaaaagc ccagaagaaa cagaaaaaga caagaatgag actgagaatg actctaaaga
     5881 tgctgagaaa aacagagaag aatttgaaga ccagtccctt gaaaaagaca gtgacgacaa
     5941 aacaccagat gatgaccctg agcaaggaaa atctgaggta ggtgatttca aatcggagaa
     6001 gtccaacggg gagctaagtg aatctcctgg agctggaaaa ggagcatctg gctcaactcg
     6061 aatcatcacc agattgcgga atccagatag caaacttagt cagctgaaga gccagcaggt
     6121 ggcagccgct gcacatgaag caaataaatt atttaaggag ggcaaagagg tactggtagt
     6181 taactctcaa ggagaaattt cacggttgag caccaaaaag gaagtgatca tgaaaggaaa
     6241 tatcaacaat tattttaaat tgggtcaaga agggaagtat cgcgtctacc acaatcaata
     6301 ctccaccaat tcatttgctt tgaataagca ccagcacaga gaagaccatg ataagagaag
     6361 gcatcttgca cataagttct gtctgactcc agcaggagag ttcaaatgga acggttctgt
     6421 ccatgggtcc aaagttctta ccatatctac tctgagactg actatcaccc aattagaaaa
     6481 caacatccct tcatcctttc ttcatcccaa ctgggcatca catagggcaa attggatcaa
     6541 ggcagttcag atgtgtagca aacccagaga atttgcattg gctttagcca ttttggagtg
     6601 tgcagttaaa ccagttgtga tgctaccaat atggcgagaa tctttaggac ataccaggtt
     6661 acaccggatg acatcaattg aaagagaaga aaaggagaaa gtcaaaaaaa aagagaagaa
     6721 acaggaagaa gaagaaacga tgcagcaagc gacatgggta aaatacacat ttccagttaa
     6781 gcatcaggtt tggaaacaaa aaggtgaaga gtacagagtg acaggatatg gtggttggag
     6841 ctggattagt aaaactcatg tttataggtt tgttcctaaa ttgccaggca atactaatgt
     6901 gaattacaga aagtcgttag aaggaaccaa aaataatatg gatgaaaata tggatgagtc
     6961 agataaaaga aaatgttcac gaagtccaaa aaaaataaaa atagagcctg attctgaaaa
     7021 agatgaggta aaaggttcag atgctgcaaa aggagcagac caaaatgaaa tggatatctc
     7081 aaagattact gagaagaagg accaagatgt gaaggagctc ttagattctg acagtgataa
     7141 accctgcaag gaagaaccaa tggaagtaga cgatgacatg aaaacagagt cacatgtaaa
     7201 ttgtcaggag agttctcaag tagatgtggt caatgttagt gagggttttc atctaaggac
     7261 tagttacaaa aagaaaacaa aatcatccaa actagatgga cttcttgaaa ggagaattaa
     7321 acagtttaca ctggaagaaa aacagcgact cgaaaaaatc aagttggagg gtggaattaa
     7381 gggtatagga aagacttcta caaattcttc aaaaaatctc tctgaatcac cagtaataac
     7441 gaaagcaaaa gaagggtgtc agagtgactc gatgagacaa gaacagagcc caaatgcaaa
     7501 taatgatcaa cctgaggact tgattcaggg atgttcagaa agtgattcct cagttcttag
     7561 aatgagtgat cctagtcata ccacaaacaa actttatcca aaagatcgag tgttagatga
     7621 tgtctccatt cggagcccag aaacaaaatg tccgaaacaa aattccattg aaaatgacat
     7681 agaagaaaaa gtctctgacc ttgccagtag aggccaggaa cccagtaaga gtaaaacaaa
     7741 aggaaatgat tttttcatcg atgactctaa actagccagt gcagatgata ttggtacttt
     7801 gatctgtaag aacaaaaaac cgctcataca ggaggaaagt gacaccattg tttcttcttc
     7861 caagagtgct ttacattcat cagtgcctaa aagtaccaat gacagagatg ccacacctct
     7921 gtcaagagca atggactttg aaggaaaact gggatgtgac tctgaatcta atagcacttt
     7981 ggaaaatagt tctgataccg tgtctattca ggatagcagt gaagaagata tgattgttca
     8041 gaatagcaat gaaagcattt ctgaacagtt cagaactcga gaacaagatg ttgaagtctt
     8101 ggagccgtta aagtgtgagt tggtttctgg tgagtccact ggaaactgtg aggacaggct
     8161 gccggtcaag gggactgaag caaatggtaa aaaaccaagt cagcagaaga aattagagga
     8221 gagaccagtt aataaatgta gtgatcaaat aaagctaaaa aataccactg acaaaaagaa
     8281 taatgaaaat cgagagtctg aaaagaaagg acagagaaca agtacatttc aaataaatgg
     8341 aaaagataat aaacccaaaa tatatttgaa aggtgaatgc ttgaaagaaa tttctgagag
     8401 tagagtagta agtggtaatg ttgaaccaaa ggttaataat ataaataaaa taatccctga
     8461 gaatgatatt aaatcattga ctgttaaaga atctgctata aggccattca ttaatggtga
     8521 tgtcatcatg gaagatttta atgaaagaaa cagctccgaa acaaaatcgc atttgctgag
     8581 ttcttcagat gctgaaggta actaccgaga tagccttgag accctgccat caaccaaaga
     8641 gtctgacagt acacagacga ccacaccctc agcatcttgt ccagaaagca attcagttaa
     8701 tcaggtagaa gatatggaaa tagaaacctc agaagttaag aaagttactt catcacctat
     8761 tacttctgaa gaggaatcta atctcagtaa tgactttatt gatgaaaatg gtctgcccat
     8821 caacaaaaat gaaaatgtca atggagaatc taaaagaaaa accgtcatca cagaagtcac
     8881 cacgatgacc tccacagtgg ccacagaatc aaaaactgtg atcaaggtag aaaaaggcga
     8941 taagcaaact gtggtttctt ccacagaaaa ttgtgcaaaa tccactgtca caaccaccac
     9001 tacaacagtg accaagcttt ccacaccctc cacaggcggc agtgtggaca tcatctctgt
     9061 aaaggagcag agcaaaaccg tggtcaccac gacagtgaca gactccctga ccaccacggg
     9121 aggcacactg gttacatcta tgactgtgag caaagagtat tccacacgag acaaagtgaa
     9181 actgatgaaa ttttcaagac caaagaagac tcgttcaggt acagctctgc catcctatag
     9241 aaaatttgtt accaagagca gcaagaagag catttttgtt ttgcctaatg atgacttaaa
     9301 aaagttggcc cgaaaaggag gaatccgaga ggtcccttat tttaattaca atgcaaaacc
     9361 tgctttggat atatggccat atccttctcc tagaccgacc tttggcatca cttggaggta
     9421 tagacttcag acagtaaagt ccttagctgg agtgagcctg atgttacggt tactgtgggc
     9481 aagtttgaga tgggatgata tggcggccaa ggctcctcca ggaggaggga ctacacggac
     9541 agaaacatcc gaaactgaaa tcacaacaac agaaataatt aagaggagag atgttggtcc
     9601 ttatggcatt cgatctgaat attgtatcag gaaaatcatt tgtcccattg gagttccaga
     9661 aacaccaaaa gaaacgccta cacctcagag gaaaggcctt cgatcaagtg cactgcggcc
     9721 aaagagacca gaaacgccca agcaaactgg ccctgttatt attgaaacct gggtagcaga
     9781 agaagaactg gaattgtggg agatcagggc atttgctgag agagtggaga aagaaaaggc
     9841 acaagcagtt gagcaacagg ctaagaaacg actggagcag cagaagccga cagtgattgc
     9901 aacttccact acttccccaa caagcagtac aaccagcacc atctctccag cacagaaggt
     9961 tatggtggcc cccataagtg gctcagttac aactggaacc aaaatggtac taactactaa
    10021 agttggatct ccagctacag taacattcca acaaaacaag aactttcatc aaacctttgc
    10081 tacatgggtt aagcaaggcc agtcaaattc aggcgttgtt caagtacagc agaaagtcct
    10141 gggtatcatt ccatcaagta caggtaccag tcagcaaacc tttacttcat tccagcccag
    10201 gacagcaaca gtcacaatta ggcccaatac ctcaggctct ggaggaacca caagcaattc
    10261 acaagtaatc acagggcctc agattcgccc tggtatgacc gtgattagaa caccactcca
    10321 acagtcaaca ctaggaaagg caattattcg aacacctgtg atggtacagc caggtgctcc
    10381 tcagcaagtg atgactcaaa tcatcagggg gcagcctgtc tccactgcag tctccgcccc
    10441 taacacggtt tcctcaacac ctgggcagaa aagcttaact tcagcaacgt ccacttcaaa
    10501 tatacagtct tcagcctcac aaccccctcg cccccaacaa ggacaagtga agctcaccat
    10561 ggctcaactt actcagttaa cacagggcca cggtggcaat caaggtttga cagtagtaat
    10621 tcaaggacaa ggtcaaacta ctggacagtt gcagttgata cctcaagggg tgactgtact
    10681 cccaggccca ggccagcagc taatgcaagc tgcaatgcca aatggtactg ttcagcgatt
    10741 cctctttacc ccattggcaa caacagccac cacagccagc accaccacca ccactgtttc
    10801 cacgacagca gcaggtacag gtgaacaaag gcagagtaaa ctgtcacccc agatgcaggt
    10861 acatcaagac aaaaccctgc caccagctca gtcatcaagt gtgggtccag cagaagccca
    10921 gccacagact gctcagcctt cagctcagcc ccagccccaa acccagcccc agtccccagc
    10981 tcagcctgaa gttcagactc agcctgaagt tcagacccaa acaactgttt catcccatgt
    11041 cccttctgaa gcacaaccca cccacgcaca gtcatccaag ccccaagttg cagcacagtc
    11101 tcagcctcaa agtaatgtcc aaggacagtc tcctgttcgt gtccaaagtc catcacagac
    11161 tcgaatacgt ccatcaactc catcccaact gtctcctgga caacaatccc aggttcagac
    11221 tacaacctca caaccgattc caattcaacc acatacatct cttcagatac cttcccaagg
    11281 ccagccacag tcacaacccc aggtacagtc ttcaactcaa actctttcat caggacaaac
    11341 tttaaatcaa gttactgttt catccccatc ccgtcctcag ctacaaatac agcagccaca
    11401 gccccaagtc attgctgtgc ctcagctgca acaacaagtc caggttctct ctcagatcca
    11461 gtcacaggtt gtggctcaga tacaggctca gcaaagtggt gtgccccagc aaatcaaact
    11521 ccagttacct atccaaattc agcaaagcag tgctgtgcag actcaccaga ttcagaatgt
    11581 ggttacagtg caggcagcca gtgtgcaaga gcagttgcaa agggttcagc aactcaggga
    11641 tcagcagcaa aagaagaaac agcaacagat agaaattaag cgtgaacaca ccctccaagc
    11701 ttctaatcaa agtgaaatca ttcagaaaca ggtggtgatg aagcataatg ctgtaataga
    11761 acatttaaaa cagaaaaaga gcatgactcc agctgaaaga gaagagaatc aaagaatgat
    11821 tgtctgtaac caggtgatga agtatatttt ggataagata gataaagaag aaaaacaggc
    11881 agcaaaaaaa cggaagcgtg aagagagtgt ggagcagaaa cgtagcaagc agaatgccac
    11941 taagctgtca gctctgctct tcaagcacaa agagcagctc agagccgaga tcctgaagaa
    12001 gagagcactc ctggacaagg atctgcaaat tgaagtgcag gaagagctga agagagacct
    12061 gaaaattaag aaagaaaaag acctgatgca gttggctcag gccacagcag tagctgcacc
    12121 ctgcccccca gtgacaccag ctcctccagc ccctccagcc cctccacctt cacctccccc
    12181 tccacctgct gtgcaacaca caggccttct gtccacgccc accttacctg ctgcttccca
    12241 gaagaggaag cgggaagagg aaaaagactc cagctcaaag tccaagaaaa agaaaatgat
    12301 ctctactacc tcaaaggaaa ctaagaagga cacaaagctt tactgtatct gtaaaacgcc
    12361 ttatgatgaa tctaaatttt atattggctg tgatcggtgt cagaattggt accatgggcg
    12421 ctgcgttggc atcttgcaaa gtgaggcaga gctcattgat gagtatgtct gtccacagtg
    12481 ccagtcaaca gaggatgcca tgacagtgct cacgccacta acagagaagg attatgaggg
    12541 gttgaagagg gtgctccgtt ccttacaggc ccataagatg gcctggcctt tccttgaacc
    12601 agtagaccct aatgatgcac cagattatta tggtgttatt aaggaaccta tggaccttgc
    12661 caccatggaa gaaagagtac aaagacgata ttatgaaaag ctgacggaat ttgtggcaga
    12721 tatgaccaaa atttttgata actgtcgtta ctacaatcca agtgactccc cattttacca
    12781 gtgtgcagaa gttctcgaat cattctttgt acagaaattg aaaggcttca aagctagcag
    12841 gtctcataac aacaaactgc agtctacagc ttcttagtaa tgaactagtc cgtaacttga
    12901 aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgagatgg gtcctactgg
    12961 atgcctcgta cgcgttaagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg
    13021 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
    13081 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
    13141 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
    13201 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
    13261 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
    13321 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
    13381 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
    13441 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
    13501 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
    13561 gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
    13621 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
    13681 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
    13741 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
    13801 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
    13861 tgtggaaaat ctctagcagt acgtatagta gttcatgtca tcttattatt cagtatttat
    13921 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg
    13981 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
    14041 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc
    14101 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
    14161 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
    14221 ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt
    14281 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
    14341 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
    14401 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
    14461 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
    14521 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
    14581 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
    14641 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
    14701 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
    14761 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
    14821 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
    14881 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
    14941 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
    15001 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
    15061 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
    15121 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
    15181 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
    15241 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
    15301 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
    15361 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
    15421 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
    15481 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
    15541 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
    15601 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
    15661 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
    15721 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
    15781 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
    15841 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
    15901 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
    15961 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
    16021 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
    16081 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
    16141 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
    16201 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
    16261 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
    16321 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
    16381 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
    16441 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
    16501 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
    16561 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
    16621 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
    16681 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
    16741 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
    16801 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
    16861 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
    16921 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
    16981 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
    17041 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
    17101 aaagggaaca aaagctggag ctgcaagc
//
