LOCUS       sequence-285237-        8864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8864)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 8864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1872
FEATURES             Location/Qualifiers
     source          1..8864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      8554..8563
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgaggggccg gcggggcagg ccgcccaagc agcccgcggc tcccgctgcg
       61 gagcgctgcg ccccggcccc gccgccaccg ccgccgccgc ccacgtccgg acccatcggg
      121 gggctccgct cgcggcaccg cggcagcagc cggggcaggt gggccgccgc ccaggctgag
      181 gtggcgccca agacgcggct gagctcgccc agggggggca gcagtagccg gaggaagccg
      241 ccgccgccgc cgccggcccc ccccagcacc agcgccccgg gccggggggg gcgaggaggc
      301 gggggcggca ggacgggggg cgggggcggc ggcggccacc tggcccggac caccgcggcc
      361 cggagggccg tcaacaaagt ggtgtacgat gaccacgaga gcgaggagga ggaggaagag
      421 gaggacatgg tctccgagga ggaggaggag gaggacggcg acgccgagga gacccaggat
      481 tctgaggacg acgaggagga tgagatggaa gaggacgacg atgactccga ttatccggag
      541 gagatggaag acgacgacga cgacgccagt tactgcacgg aaagcagctt caggagccat
      601 agtacctaca gcagcactcc aggtaggcga aaaccaagag tacatcggcc tcgttctcct
      661 atattggaag aaaaagacat cccgcccctt gaatttccca agtcctctga ggatttaatg
      721 gtgcctaatg agcatataat gaatgtcatt gccatttacg aggtactgcg gaactttggc
      781 actgttttga gattatctcc ttttcgcttt gaggactttt gtgcagctct ggtgagccaa
      841 gagcagtgca cactcatggc agagatgcat gttgtgcttt tgaaagcagt tctgcgtgaa
      901 gaagacactt ccaatactac ctttggacct gctgatctga aagatagcgt taattccaca
      961 ctgtatttca tagatgggat gacgtggcca gaggtgctgc gggtgtactg tgagagtgat
     1021 aaggagtacc atcacgttct tccttaccaa gaggcagagg actacccata tggaccagta
     1081 gagaacaaga tcaaagttct acagtttcta gtcgatcagt ttcttacaac aaatattgct
     1141 cgagaggaat tgatgtctga aggggtgata cagtatgatg accattgtag ggtttgtcac
     1201 aaacttgggg atttgctttg ctgtgagaca tgttcagcag tataccattt ggaatgtgtg
     1261 aagccacctc ttgaggaggt gccagaggac gagtggcagt gtgaagtctg tgtagcacac
     1321 aaggtgcctg gtgtgactga ctgtgttgct gaaatccaaa aaaataaacc atatattcga
     1381 catgaaccta ttggatatga tagaagtcgg aggaaatact ggttcttgaa ccgaagactc
     1441 ataatagaag aagatacaga aaatgaaaat gaaaagaaaa tttggtatta cagcacaaag
     1501 gtccaacttg cagaattaat tgactgtcta gacaaagatt attgggaagc agaactctgc
     1561 aaaattctag aagaaatgcg tgaagaaatc caccgacaca tggacataac tgaagacctg
     1621 accaataagg ctcggggcag taacaaatcc tttctggcgg cagctaatga agaaattttg
     1681 gaatccataa gagccaaaaa gggagacatt gataatgtta aaagcccaga agaaacagaa
     1741 aaagacaaga atgagactga gaatgactct aaagatgctg agaaaaacag agaagaattt
     1801 gaagaccagt cccttgaaaa agacagtgac gacaaaacac cagatgatga ccctgagcaa
     1861 ggaaaatctg aggtaggtga tttcaaatcg gagaagtcca acggggagct aagtgaatct
     1921 cctggagctg gaaaaggagc atctggctca actcgaatca tcaccagatt gcggaatcca
     1981 gatagcaaac ttagtcagct gaagagccag caggtggcag ccgctgcaca tgaagcaaat
     2041 aaattattta aggagggcaa agaggtactg gtagttaact ctcaaggaga aatttcacgg
     2101 ttgagcacca aaaaggaagt gatcatgaaa ggaaatatca acaattattt taaattgggt
     2161 caagaaggga agtatcgcgt ctaccacaat caatactcca ccaattcatt tgctttgaat
     2221 aagcaccagc acagagaaga ccatgataag agaaggcatc ttgcacataa gttctgtctg
     2281 actccagcag gagagttcaa atggaacggt tctgtccatg ggtccaaagt tcttaccata
     2341 tctactctga gactgactat cacccaatta gaaaacaaca tcccttcatc ctttcttcat
     2401 cccaactggg catcacatag ggcaaattgg atcaaggcag ttcagatgtg tagcaaaccc
     2461 agagaatttg cattggcttt agccattttg gagtgtgcag ttaaaccagt tgtgatgcta
     2521 ccaatatggc gagaatcttt aggacatacc aggttacacc ggatgacatc aattgaaaga
     2581 gaagaaaagg agaaagtcaa aaaaaaagag aagaaacagg aagaagaaga aacgatgcag
     2641 caagcgacat gggtaaaata cacatttcca gttaagcatc aggtttggaa acaaaaaggt
     2701 gaagagtaca gagtgacagg atatggtggt tggagctgga ttagtaaaac tcatgtttat
     2761 aggtttgttc ctaaattgcc aggcaatact aatgtgaatt acagaaagtc gttagaagga
     2821 accaaaaata atatggatga aaatatggat gagtcagata aaagaaaatg ttcacgaagt
     2881 ccaaaaaaaa taaaaataga gcctgattct gaaaaagatg aggtaaaagg ttcagatgct
     2941 gcaaaaggag cagaccaaaa tgaaatggat atctcaaaga ttactgagaa gaaggaccaa
     3001 gatgtgaagg agctcttaga ttctgacagt gataaaccct gcaaggaaga accaatggaa
     3061 gtagacgatg acatgaaaac agagtcacat gtaaattgtc aggagagttc tcaagtagat
     3121 gtggtcaatg ttagtgaggg ttttcatcta aggactagtt acaaaaagaa aacaaaatca
     3181 tccaaactag atggacttct tgaaaggaga attaaacagt ttacactgga agaaaaacag
     3241 cgactcgaaa aaatcaagtt ggagggtgga attaagggta taggaaagac ttctacaaat
     3301 tcttcaaaaa atctctctga atcaccagta ataacgaaag caaaagaagg gtgtcagagt
     3361 gactcgatga gacaagaaca gagcccaaat gcaaataatg atcaacctga ggacttgatt
     3421 cagggatgtt cagaaagtga ttcctcagtt cttagaatga gtgatcctag tcataccaca
     3481 aacaaacttt atccaaaaga tcgagtgtta gatgatgtct ccattcggag cccagaaaca
     3541 aaatgtccga aacaaaattc cattgaaaat gacatagaag aaaaagtctc tgaccttgcc
     3601 agtagaggcc aggaacccag taagagtaaa acaaaaggaa atgatttttt catcgatgac
     3661 tctaaactag ccagtgcaga tgatattggt actttgatct gtaagaacaa aaaaccgctc
     3721 atacaggagg aaagtgacac cattgtttct tcttccaaga gtgctttaca ttcatcagtg
     3781 cctaaaagta ccaatgacag agatgccaca cctctgtcaa gagcaatgga ctttgaagga
     3841 aaactgggat gtgactctga atctaatagc actttggaaa atagttctga taccgtgtct
     3901 attcaggata gcagtgaaga agatatgatt gttcagaata gcaatgaaag catttctgaa
     3961 cagttcagaa ctcgagaaca agatgttgaa gtcttggagc cgttaaagtg tgagttggtt
     4021 tctggtgagt ccactggaaa ctgtgaggac aggctgccgg tcaaggggac tgaagcaaat
     4081 ggtaaaaaac caagtcagca gaagaaatta gaggagagac cagttaataa atgtagtgat
     4141 caaataaagc taaaaaatac cactgacaaa aagaataatg aaaatcgaga gtctgaaaag
     4201 aaaggacaga gaacaagtac atttcaaata aatggaaaag ataataaacc caaaatatat
     4261 ttgaaaggtg aatgcttgaa agaaatttct gagagtagag tagtaagtgg taatgttgaa
     4321 ccaaaggtta ataatataaa taaaataatc cctgagaatg atattaaatc attgactgtt
     4381 aaagaatctg ctataaggcc attcattaat ggtgatgtca tcatggaaga ttttaatgaa
     4441 agaaacagct ccgaaacaaa atcgcatttg ctgagttctt cagatgctga aggtaactac
     4501 cgagatagcc ttgagaccct gccatcaacc aaagagtctg acagtacaca gacgaccaca
     4561 ccctcagcat cttgtccaga aagcaattca gttaatcagg tagaagatat ggaaatagaa
     4621 acctcagaag ttaagaaagt tacttcatca cctattactt ctgaagagga atctaatctc
     4681 agtaatgact ttattgatga aaatggtctg cccatcaaca aaaatgaaaa tgtcaatgga
     4741 gaatctaaaa gaaaaaccgt catcacagaa gtcaccacga tgacctccac agtggccaca
     4801 gaatcaaaaa ctgtgatcaa ggtagaaaaa ggcgataagc aaactgtggt ttcttccaca
     4861 gaaaattgtg caaaatccac tgtcacaacc accactacaa cagtgaccaa gctttccaca
     4921 ccctccacag gcggcagtgt ggacatcatc tctgtaaagg agcagagcaa aaccgtggtc
     4981 accacgacag tgacagactc cctgaccacc acgggaggca cactggttac atctatgact
     5041 gtgagcaaag agtattccac acgagacaaa gtgaaactga tgaaattttc aagaccaaag
     5101 aagactcgtt caggtacagc tctgccatcc tatagaaaat ttgttaccaa gagcagcaag
     5161 aagagcattt ttgttttgcc taatgatgac ttaaaaaagt tggcccgaaa aggaggaatc
     5221 cgagaggtcc cttattttaa ttacaatgca aaacctgctt tggatatatg gccatatcct
     5281 tctcctagac cgacctttgg catcacttgg aggtatagac ttcagacagt aaagtcctta
     5341 gctggagtga gcctgatgtt acggttactg tgggcaagtt tgagatggga tgatatggcg
     5401 gccaaggctc ctccaggagg agggactaca cggacagaaa catccgaaac tgaaatcaca
     5461 acaacagaaa taattaagag gagagatgtt ggtccttatg gcattcgatc tgaatattgt
     5521 atcaggaaaa tcatttgtcc cattggagtt ccagaaacac caaaagaaac gcctacacct
     5581 cagaggaaag gccttcgatc aagtgcactg cggccaaaga gaccagaaac gcccaagcaa
     5641 actggccctg ttattattga aacctgggta gcagaagaag aactggaatt gtgggagatc
     5701 agggcatttg ctgagagagt ggagaaagaa aaggcacaag cagttgagca acaggctaag
     5761 aaacgactgg agcagcagaa gccgacagtg attgcaactt ccactacttc cccaacaagc
     5821 agtacaacca gcaccatctc tccagcacag aaggttatgg tggcccccat aagtggctca
     5881 gttacaactg gaaccaaaat ggtactaact actaaagttg gatctccagc tacagtaaca
     5941 ttccaacaaa acaagaactt tcatcaaacc tttgctacat gggttaagca aggccagtca
     6001 aattcaggcg ttgttcaagt acagcagaaa gtcctgggta tcattccatc aagtacaggt
     6061 accagtcagc aaacctttac ttcattccag cccaggacag caacagtcac aattaggccc
     6121 aatacctcag gctctggagg aaccacaagc aattcacaag taatcacagg gcctcagatt
     6181 cgccctggta tgaccgtgat tagaacacca ctccaacagt caacactagg aaaggcaatt
     6241 attcgaacac ctgtgatggt acagccaggt gctcctcagc aagtgatgac tcaaatcatc
     6301 agggggcagc ctgtctccac tgcagtctcc gcccctaaca cggtttcctc aacacctggg
     6361 cagaaaagct taacttcagc aacgtccact tcaaatatac agtcttcagc ctcacaaccc
     6421 cctcgccccc aacaaggaca agtgaagctc accatggctc aacttactca gttaacacag
     6481 ggccacggtg gcaatcaagg tttgacagta gtaattcaag gacaaggtca aactactgga
     6541 cagttgcagt tgatacctca aggggtgact gtactcccag gcccaggcca gcagctaatg
     6601 caagctgcaa tgccaaatgg tactgttcag cgattcctct ttaccccatt ggcaacaaca
     6661 gccaccacag ccagcaccac caccaccact gtttccacga cagcagcagg tacaggtgaa
     6721 caaaggcaga gtaaactgtc accccagatg caggtacatc aagacaaaac cctgccacca
     6781 gctcagtcat caagtgtggg tccagcagaa gcccagccac agactgctca gccttcagct
     6841 cagccccagc cccaaaccca gccccagtcc ccagctcagc ctgaagttca gactcagcct
     6901 gaagttcaga cccaaacaac tgtttcatcc catgtccctt ctgaagcaca acccacccac
     6961 gcacagtcat ccaagcccca agttgcagca cagtctcagc ctcaaagtaa tgtccaagga
     7021 cagtctcctg ttcgtgtcca aagtccatca cagactcgaa tacgtccatc aactccatcc
     7081 caactgtctc ctggacaaca atcccaggtt cagactacaa cctcacaacc gattccaatt
     7141 caaccacata catctcttca gataccttcc caaggccagc cacagtcaca accccaggta
     7201 cagtcttcaa ctcaaactct ttcatcagga caaactttaa atcaagttac tgtttcatcc
     7261 ccatcccgtc ctcagctaca aatacagcag ccacagcccc aagtcattgc tgtgcctcag
     7321 ctgcaacaac aagtccaggt tctctctcag atccagtcac aggttgtggc tcagatacag
     7381 gctcagcaaa gtggtgtgcc ccagcaaatc aaactccagt tacctatcca aattcagcaa
     7441 agcagtgctg tgcagactca ccagattcag aatgtggtta cagtgcaggc agccagtgtg
     7501 caagagcagt tgcaaagggt tcagcaactc agggatcagc agcaaaagaa gaaacagcaa
     7561 cagatagaaa ttaagcgtga acacaccctc caagcttcta atcaaagtga aatcattcag
     7621 aaacaggtgg tgatgaagca taatgctgta atagaacatt taaaacagaa aaagagcatg
     7681 actccagctg aaagagaaga gaatcaaaga atgattgtct gtaaccaggt gatgaagtat
     7741 attttggata agatagataa agaagaaaaa caggcagcaa aaaaacggaa gcgtgaagag
     7801 agtgtggagc agaaacgtag caagcagaat gccactaagc tgtcagctct gctcttcaag
     7861 cacaaagagc agctcagagc cgagatcctg aagaagagag cactcctgga caaggatctg
     7921 caaattgaag tgcaggaaga gctgaagaga gacctgaaaa ttaagaaaga aaaagacctg
     7981 atgcagttgg ctcaggccac agcagtagct gcaccctgcc ccccagtgac accagctcct
     8041 ccagcccctc cagcccctcc accttcacct ccccctccac ctgctgtgca acacacaggc
     8101 cttctgtcca cgcccacctt acctgctgct tcccagaaga ggaagcggga agaggaaaaa
     8161 gactccagct caaagtccaa gaaaaagaaa atgatctcta ctacctcaaa ggaaactaag
     8221 aaggacacaa agctttactg tatctgtaaa acgccttatg atgaatctaa attttatatt
     8281 ggctgtgatc ggtgtcagaa ttggtaccat gggcgctgcg ttggcatctt gcaaagtgag
     8341 gcagagctca ttgatgagta tgtctgtcca cagtgccagt caacagagga tgccatgaca
     8401 gtgctcacgc cactaacaga gaaggattat gaggggttga agagggtgct ccgttcctta
     8461 caggcccata agatggcctg gcctttcctt gaaccagtag accctaatga tgcaccagat
     8521 tattatggtg ttattaagga acctatggac cttgccacca tggaagaaag agtacaaaga
     8581 cgatattatg aaaagctgac ggaatttgtg gcagatatga ccaaaatttt tgataactgt
     8641 cgttactaca atccaagtga ctccccattt taccagtgtg cagaagttct cgaatcattc
     8701 tttgtacaga aattgaaagg cttcaaagct agcaggtctc ataacaacaa actgcagtct
     8761 acagcttctt agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     8821 atatcttgtg gaaaggacga gatgggtcct actggatgcc tcgt
//
