LOCUS       sequence-285242-       12436 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12436)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12436)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1995
FEATURES             Location/Qualifiers
     source          1..12436
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    8293..8881
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8346..8366)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8764..8775)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8953..9186
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9264..9385
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9301..9320)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9355..9374
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9425..9560
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9580..9599)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9581..9599)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9609..9626)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9609..9625)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9618..9640)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9767..10222
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9854..9873)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10064..10085
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10248..10352
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10353..10421,10422..11213)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10571..10590)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11384..11972
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11873..11892
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12126..12143
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12260..12281
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12296..12301,12302..12319,12320..12326)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12334..12350
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12339..12361
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12358..12374
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12358..12374
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12393..12413
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12395..12413
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgcag agcaaggtgc tgctggccgt
     4141 cgccctgtgg ctctgcgtgg agacccgggc cgcctctgtg ggtttgccta gtgtttctct
     4201 tgatctgccc aggctcagca tacaaaaaga catacttaca attaaggcta atacaactct
     4261 tcaaattact tgcaggggac agagggactt ggactggctt tggcccaata atcagagtgg
     4321 cagtgagcaa agggtggagg tgactgagtg cagcgatggc ctcttctgta agacactcac
     4381 aattccaaaa gtgatcggaa atgacactgg agcctacaag tgcttctacc gggaaactga
     4441 cttggcctcg gtcatttatg tctatgttca agattacaga tctccattta ttgcttctgt
     4501 tagtgaccaa catggagtcg tgtacattac tgagaacaaa aacaaaactg tggtgattcc
     4561 atgtctcggg tccatttcaa atctcaacgt gtcactttgt gcaagatacc cagaaaagag
     4621 atttgttcct gatggtaaca gaatttcctg ggacagcaag aagggcttta ctattcccag
     4681 ctacatgatc agctatgctg gcatggtctt ctgtgaagca aaaattaatg atgaaagtta
     4741 ccagtctatt atgtacatag ttgtcgttgt agggtatagg atttatgatg tggttctgag
     4801 tccgtctcat ggaattgaac tatctgttgg agaaaagctt gtcttaaatt gtacagcaag
     4861 aactgaacta aatgtgggga ttgacttcaa ctgggaatac ccttcttcga agcatcagca
     4921 taagaaactt gtaaaccgag acctaaaaac ccagtctggg agtgagatga agaaattttt
     4981 gagcacctta actatagatg gtgtaacccg gagtgaccaa ggattgtaca cctgtgcagc
     5041 atccagtggg ctgatgacca agaagaacag cacatttgtc agggtccatg aaaaaccttt
     5101 tgttgctttt ggaagtggca tggaatctct ggtggaagcc acggtggggg agcgtgtcag
     5161 aatccctgcg aagtaccttg gttacccacc cccagaaata aaatggtata aaaatggaat
     5221 accccttgag tccaatcaca caattaaagc ggggcatgta ctgacgatta tggaagtgag
     5281 tgaaagagac acaggaaatt acactgtcat ccttaccaat cccatttcaa aggagaagca
     5341 gagccatgtg gtctctctgg ttgtgtatgt cccaccccag attggtgaga aatctctaat
     5401 ctctcctgtg gattcctacc agtacggcac cactcaaacg ctgacatgta cggtctatgc
     5461 cattcctccc ccgcatcaca tccactggta ttggcagttg gaggaagagt gcgccaacga
     5521 gcccagccaa gctgtctcag tgacaaaccc atacccttgt gaagaatgga gaagtgtgga
     5581 ggacttccag ggaggaaata aaattgaagt taataaaaat caatttgctc taattgaagg
     5641 aaaaaacaaa actgtaagta cccttgttat ccaagcggca aatgtgtcag ctttgtacaa
     5701 atgtgaagcg gtcaacaaag tcgggagagg agagagggtg atctccttcc acgtgaccag
     5761 gggtcctgaa attactttgc aacctgacat gcagcccact gagcaggaga gcgtgtcttt
     5821 gtggtgcact gcagacagat ctacgtttga gaacctcaca tggtacaagc ttggcccaca
     5881 gcctctgcca atccatgtgg gagagttgcc cacacctgtt tgcaagaact tggatactct
     5941 ttggaaattg aatgccacca tgttctctaa tagcacaaat gacattttga tcatggagct
     6001 taagaatgca tccttgcagg accaaggaga ctatgtctgc cttgctcaag acaggaagac
     6061 caagaaaaga cattgcgtgg tcaggcagct cacagtccta gagcgtgtgg cacccacgat
     6121 cacaggaaac ctggagaatc agacgacaag tattggggaa agcatcgaag tctcatgcac
     6181 ggcatctggg aatccccctc cacagatcat gtggtttaaa gataatgaga cccttgtaga
     6241 agactcaggc attgtattga aggatgggaa ccggaacctc actatccgca gagtgaggaa
     6301 ggaggacgaa ggcctctaca cctgccaggc atgcagtgtt cttggctgtg caaaagtgga
     6361 ggcatttttc ataatagaag gtgcccagga aaagacgaac ttggaaatca ttattctagt
     6421 aggcacggcg gtgattgcca tgttcttctg gctacttctt gtcatcatcc tacggaccgt
     6481 taagcgggcc aatggagggg aactgaagac aggctacttg tccatcgtca tggatccaga
     6541 tgaactccca ttggatgaac attgtgaacg actgccttat gatgccagca aatgggaatt
     6601 ccccagagac cggctgaagc taggtaagcc tcttggccgt ggtgcctttg gccaagtgat
     6661 tgaagcagat gcctttggaa ttgacaagac agcaacttgc aggacagtag cagtcaaaat
     6721 gttgaaagaa ggagcaacac acagtgagca tcgagctctc atgtctgaac tcaagatcct
     6781 cattcatatt ggtcaccatc tcaatgtggt caaccttcta ggtgcctgta ccaagccagg
     6841 agggccactc atggtgattg tggaattctg caaatttgga aacctgtcca cttacctgag
     6901 gagcaagaga aatgaatttg tcccctacaa gaccaaaggg gcacgattcc gtcaagggaa
     6961 agactacgtt ggagcaatcc ctgtggatct gaaacggcgc ttggacagca tcaccagtag
     7021 ccagagctca gccagctctg gatttgtgga ggagaagtcc ctcagtgatg tagaagaaga
     7081 ggaagctcct gaagatctgt ataaggactt cctgaccttg gagcatctca tctgttacag
     7141 cttccaagtg gctaagggca tggagttctt ggcatcgcga aagtgtatcc acagggacct
     7201 ggcggcacga aatatcctct tatcggagaa gaacgtggtt aaaatctgtg actttggctt
     7261 ggcccgggat atttataaag atccagatta tgtcagaaaa ggagatgctc gcctcccttt
     7321 gaaatggatg gccccagaaa caatttttga cagagtgtac acaatccaga gtgacgtctg
     7381 gtcttttggt gttttgctgt gggaaatatt ttccttaggt gcttctccat atcctggggt
     7441 aaagattgat gaagaatttt gtaggcgatt gaaagaagga actagaatga gggcccctga
     7501 ttatactaca ccagaaatgt accagaccat gctggactgc tggcacgggg agcccagtca
     7561 gagacccacg ttttcagagt tggtggaaca tttgggaaat ctcttgcaag ctaatgctca
     7621 gcaggatggc aaagactaca ttgttcttcc gatatcagag actttgagca tggaagagga
     7681 ttctggactc tctctgccta cctcacctgt ttcctgtatg gaggaggagg aagtatgtga
     7741 ccccaaattc cattatgaca acacagcagg aatcagtcag tatctgcaga acagtaagcg
     7801 aaagagccgg cctgtgagtg taaaaacatt tgaagatatc ccgttagaag aaccagaagt
     7861 aaaagtaatc ccagatgaca accagacgga cagtggtatg gttcttgcct cagaagagct
     7921 gaaaactttg gaagacagaa ccaaattatc tccatctttt ggtggaatgg tgcccagcaa
     7981 aagcagggag tctgtggcat ctgaaggctc aaaccagaca agcggctacc agtccggata
     8041 tcactccgat gacacagaca ccaccgtgta ctccagtgag gaagcagaac ttttaaagct
     8101 gatagagatt ggagtgcaaa ccggtagcac agcccagatt ctccagcctg actcggggac
     8161 cacactgagc tctcctcctg tttagtaatg aactagtccg taacttgaaa gtatttcgat
     8221 ttcttggctt tatatatctt gtggaaagga cgaaccaggt atttgtatca gaacctgacg
     8281 cgttaagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     8341 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     8401 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     8461 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     8521 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     8581 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     8641 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     8701 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     8761 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     8821 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     8881 cgtcgacttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     8941 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     9001 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     9061 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     9121 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     9181 ctagcagtac gtatagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     9241 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
     9301 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     9361 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc
     9421 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     9481 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     9541 aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac
     9601 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     9661 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     9721 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc
     9781 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     9841 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     9901 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     9961 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
    10021 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
    10081 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
    10141 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
    10201 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta
    10261 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
    10321 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
    10381 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
    10441 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
    10501 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
    10561 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
    10621 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
    10681 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
    10741 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
    10801 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
    10861 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
    10921 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
    10981 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
    11041 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
    11101 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
    11161 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
    11221 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
    11281 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
    11341 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
    11401 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
    11461 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
    11521 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
    11581 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
    11641 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
    11701 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
    11761 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
    11821 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
    11881 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
    11941 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
    12001 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
    12061 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
    12121 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
    12181 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
    12241 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
    12301 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
    12361 gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa
    12421 agctggagct gcaagc
//
