LOCUS       sequence-285261-        4457 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4457)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 4457)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2091
FEATURES             Location/Qualifiers
     source          1..4457
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggtttccaa aagaagactg tcaaaatctg aggataaaga gagcctgaca
       61 gaagatgcct ccaaaaccag gaagcaacca ctttccaaaa agacaaagaa atctcatatt
      121 gctaatgaag ttgaagaaaa tgacagcatc tttgtaaagc ttcttaagat atcaggaatt
      181 attcttaaaa cgggagagag tcagaatcaa ctagctgtgg atcaaatagc tttccaaaag
      241 aagctctttc agaccctgag gagacaccct tcctatccca aaataataga agaatttgtt
      301 agtggcctgg agtcttacat tgaggatgaa gacagtttca ggaactgcct tttgtcttgt
      361 gagcgtctgc aggatgagga agccagtatg ggtgcatctt attctaagag tctcatcaaa
      421 ctgcttctgg ggattgacat actgcagcct gccattatca aaaccttatt tgagaagttg
      481 ccagaatatt tttttgaaaa caagaacagt gatgaaatca acatacctcg actcattgtc
      541 agtcaactaa aatggcttga cagagttgtg gatggcaagg acctcaccac caagatcatg
      601 cagctgatca gtattgctcc agagaacctg cagcatgaca tcatcaccag cctacctgag
      661 atcctagggg attcccagca cgctgatgtg gggaaagaac tcagtgacct actgatagag
      721 aatacttcac tcactgtccc aatcctggat gtcctttcaa gcctccgact tgacccaaac
      781 ttcctattga aggttcgcca gttggtgatg gataagttgt cgtctattag attggaggat
      841 ttacctgtga taataaagtt cattcttcat tccgtaacag ccatggatac acttgaggta
      901 atttctgagc ttcgggagaa gttggatctg cagcattgtg ttttgccatc acggttacag
      961 gcttcccaag taaagttgaa aagtaaagga cgagcaagtt cctcaggaaa tcaagaaagc
     1021 agcggtcaga gctgtattat tctcctcttt gatgtaataa agtcagctat tagatatgag
     1081 aaaaccattt cagaagcctg gattaaggca attgaaaaca ctgcctcagt atctgaacac
     1141 aaggtgtttg acctggtgat gcttttcatc atctatagca ccaatactca gacaaagaag
     1201 tacattgaca gggtgctaag aaataagatt cgatcaggct gcattcaaga acagctgctc
     1261 cagagtacat tctctgttca ttacttagtt cttaaggata tgtgttcatc cattctgtcg
     1321 ctggctcaga gtttgcttca ctctctagac cagagtataa tttcatttgg cagtctccta
     1381 tacaaatatg catttaagtt ttttgacacg tactgccagc aggaagtggt tggtgcctta
     1441 gtgacccata tctgcagtgg gaatgaagct gaagttgata ctgccttaga tgtccttcta
     1501 gagttggtag tgttaaaccc atctgctatg atgatgaatg ctgtctttgt aaagggcatt
     1561 ttagattatc tggataacat atcccctcag caaatacgaa aactcttcta tgttctcagc
     1621 acactggcat ttagcaaaca gaatgaagcc agcagccaca tccaggatga catgcacttg
     1681 gtgataagaa agcagctctc tagcaccgta ttcaagtaca agctcattgg gattattggt
     1741 gctgtgacca tggctggcat catggcggca gacagaagtg aatcacctag tttgacccaa
     1801 gagagagcca acctgagcga tgagcagtgc acacaggtga cctccttgtt gcagttggtt
     1861 cattcctgca gtgagcagtc tcctcaggcc tctgcacttt actatgatga atttgccaac
     1921 ctgatccaac atgaaaagct ggatccaaaa gccctggaat gggttgggca taccatctgt
     1981 aatgatttcc aggatgcctt cgtagtggac tcctgtgttg ttccggaagg tgactttcca
     2041 tttcctgtga aagcactgta cggactggaa gaatacgaca ctcaggatgg gattgccata
     2101 aacctcctgc cgctgctgtt ttctcaggac tttgcaaaag atgggggtcc ggtgacctca
     2161 caggaatcag gccaaaaatt ggtgtctccg ctgtgcctgg ctccgtattt ccggttactg
     2221 agactttgtg tggagagaca gcataacgga aacttggagg agattgatgg tctactagat
     2281 tgtcctatat tcctaactga cctggagcct ggagagaagt tggagtccat gtctgctaaa
     2341 gagcgttcat tcatgtgttc tctcatattt cttactctca actggttccg agagattgta
     2401 aatgccttct gccaggaaac atcacctgag atgaagggga aggtgctcac tcggttaaag
     2461 cacattgtag aattgcaaat aatcctggaa aagtacttgg cagtcacccc agactatgtc
     2521 cctcctcttg gaaactttga tgtggaaact ttagatataa cacctcatac tgttactgct
     2581 atttcagcaa aaatcagaaa gaaaggaaaa atagaaagga aacaaaaaac agatggcagc
     2641 aagacatcct cctctgacac actttcagaa gagaaaaatt cagaatgtga ccctacgcca
     2701 tctcatagag gccagctaaa caaggagttc acagggaagg aagaaaagac atcattgtta
     2761 ctacataatt cccatgcttt tttccgagag ctggacattg aggtcttctc tattctacat
     2821 tgtggacttg tgacgaagtt catcttagat actgaaatgc acactgaagc tacagaagtt
     2881 gtgcaacttg ggccccctga gctgcttttc ttgctggaag atctctccca gaagctggag
     2941 agtatgctga cacctcctat tgccaggaga gtcccctttc tcaagaacaa aggaagccgg
     3001 aatattggat tctcacatct ccaacagaga tctgcccaag aaattgttca ttgtgttttt
     3061 caactgctga ccccaatgtg taaccacctg gagaacattc acaactattt tcagtgttta
     3121 gctgctgaga atcacggtgt agttgatgga ccaggagtga aagttcagga gtaccacata
     3181 atgtcttcct gctatcagag gctgctgcag atttttcatg ggctttttgc ttggagtgga
     3241 ttttctcaac ctgaaaatca gaatttactg tattcagccc tccatgtcct tagtagccga
     3301 ctgaaacagg gagaacacag ccagcctttg gaggaactac tcagccagag cgtccattac
     3361 ttgcagaatt tccatcaaag cattcccagt ttccagtgtg ctctttatct catcagactt
     3421 ttgatggtta ttttggagaa atcaacagct tctgctcaga acaaagaaaa aattgcttcc
     3481 cttgccagac aattcctctg tcgggtgtgg ccaagtgggg ataaagagaa gagcaacatc
     3541 tctaatgacc agctccatgc tctgctctgt atctacctgg agcacacaga gagcattctg
     3601 aaggccatag aggagattgc tggtgttggt gtcccagaac tgatcaactc tcctaaagat
     3661 gcatcttcct ccacattccc tacactgacc aggcatactt ttgttgtttt cttccgtgtg
     3721 atgatggctg aactagagaa gacggtgaaa aaaattgagc ctggcacagc agcagactcg
     3781 cagcagattc atgaagagaa actcctctac tggaacatgg ctgttcgaga cttcagtatc
     3841 ctcatcaact tgataaaggt atttgatagt catcctgttc tgcatgtatg tttgaagtat
     3901 gggcgtctct ttgtggaagc atttctgaag caatgtatgc cgctcctaga cttcagtttt
     3961 agaaaacacc gggaagatgt tctgagctta ctggaaacct tccagttgga cacaaggctg
     4021 cttcatcacc tgtgtgggca ttccaagatt caccaggaca cgagactcac ccaacatgtg
     4081 cctctgctca aaaagaccct ggaactttta gtttgcagag tcaaagctat gctcactctc
     4141 aacaattgta gagaggcttt ctggctgggc aatctaaaaa accgggactt gcagggtgaa
     4201 gagattaagt cccaaaattc ccaggagagc acagcagatg agagtgagga tgacatgtca
     4261 tcccaggcct ccaagagcaa agccactgag gatggtgaag aagacgaagt aagtgctgga
     4321 gaaaaggagc aagatagtga tgagagttat gatgactctg attagtaatg aactagtccg
     4381 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgacttggct
     4441 gtgcatcaaa tcaaatc
//
