LOCUS       sequence-285267-        3314 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3314)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2115
FEATURES             Location/Qualifiers
     source          1..3314
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggttttgga tgaccttcca aacttagaag acatctatac ttccttgtgt
       61 tcatcaacaa tggaagactc agagatggat tttgactctg gactagaaga tgatgacaca
      121 aaaagtgata gtattttgga ggattccaca atttttgtgg ccttcaaagg aaatatagat
      181 gataaagact tcaaatggaa attagatgca atattgaaaa acgtgcccaa tttgttacac
      241 atggagtcca gcaagctaaa agtacagaag gtggagccct ggaacagcgt gcgtgtgaca
      301 ttcaacatcc cccgggaagc agcggagcgg ctacggatcc ttgctcagag caacaaccag
      361 cagcttcggg atttagggat tctctccgtt cagattgaag gggaaggtgc tattaacctg
      421 gctttggctc agaaccgaag ccaagatgtg agaatgaatg gacccatggg agctggaaat
      481 tcagttagga tggaggcggg atttcctatg gcaagtggtc caggaataat aaggatgaac
      541 aaccctgcca ctgttatgat acccccgggt ggaaatgtgt catcttccat gatggcacca
      601 ggccccaatc cagagctgca gcccaggact cctcgccctg cttctcagtc agatgcaatg
      661 gatccactcc tctctgggct ccatatacag cagcaaagtc atccctcagg atctttagct
      721 cccccacatc acccaatgca gcctgtctct gtgaacagac aaatgaaccc agctaatttt
      781 ccccagctgc agcagcagca gcaacaacaa caacagcagc agcagcagca gcagcagcaa
      841 caacagcaac agcagcaaca acagttgcag gcaagacccc cacagcaaca tcagcagcaa
      901 cagccacagg gaattcgacc ccagtttact gccccaactc aggtgcctgt tcctccaggc
      961 tggaaccagc tgccttctgg agcccttcaa cctcctccag cccagggttc tctgggcaca
     1021 atgactgcaa accaagggtg gaagaaggct cccttgcccg gcccaatgca acagcaactc
     1081 caggcaagac catccttagc cacggtacag acgccttccc accctccccc tccatatccc
     1141 tttggcagcc agcaagcctc acaagcccac acaaactttc ctcagatgag caacccaggc
     1201 cagttcacag ctcctcagat gaagagtttg cagggagggc cctctagggt cccaactccc
     1261 ttgcagcagc cccacctcac caacaagtct cctgcctcct caccctcctc cttccagcag
     1321 ggatcccctg catcctcccc aacggttaac caaactcagc agcagatggg accaaggcca
     1381 cctcaaaata acccacttcc ccagggattt cagcagcctg tcagctctcc gggtcggaat
     1441 cctatggttc aacagggaaa tgtgccacct aacttcatgg tgatgcagca gcaaccacca
     1501 aaccaggggc cacagagttt acatccaggc ctaggaggaa tgcctaaacg cctcccacct
     1561 ggcttctcag caggacaggc caatccgaac tttatgcaag gtcaggtgcc ttcgaccaca
     1621 gcaaccaccc ctgggaattc aggagcccct cagctgcaag caaatcaaaa tgtccagcat
     1681 gcaggtggtc aaggagctgg tcctcctcaa aaccagatgc aggtgtccca cgggccgcca
     1741 aatatgatgc agcccagcct catgggaatt catggcaaca tgaacaatca gcaggctggt
     1801 acttctgggg ttcctcaagt gaacctcagc aacatgcaag gccagcccca gcagggccca
     1861 ccatctcagc tgatgggcat gcaccagcaa atcgtgccct cccagggcca gatggtccag
     1921 caacaaggaa ccttgaaccc tcagaaccct atgatccttt caagggccca gcttatgcca
     1981 cagggccaga tgatggtgaa ccccccgagc caaaatcttg ggccctcgcc ccaaaggatg
     2041 accccaccca agcagatgct ttcccagcag ggcccacaaa tgatggcgcc acataaccag
     2101 atgatggggc ctcaggggca ggttttgctc caacagaacc caatgataga gcagattatg
     2161 accaatcaaa tgcaggggaa taagcagcag tttaacactc agaaccagtc caatgtcatg
     2221 ccgggaccag cccagataat gaggggacca actccaaaca tgcaaggaaa tatggtgcag
     2281 tttacgggac agatgtcagg acagatgctg ccccagcaag ggcctgtgaa caacagtcca
     2341 tctcaggtta tgggcattca gggacaggtc ctgcggccac cagggcccag cccacacatg
     2401 gcccagcagc atggtgatcc tgctactaca gcaaataacg atgtcagttt atctcagatg
     2461 atgcctgatg ttagcattca acaaaccaac atggtccccc ctcatgtgca ggccatgcag
     2521 ggaaacagtg cctcgggaaa ccacttctca ggccatggga tgtctttcaa tgcacctttc
     2581 agtggagctc ccaatggaaa tcagatgtcc tgtggtcaaa atccaggctt cccagtcaat
     2641 aaggatgtca cgctaacgag cccattgttg gtcaacttat tgcagagtga catatctgca
     2701 ggccattttg gggtaaacaa taagcaaaat aataccaacg caaataaacc gaagaagaag
     2761 aaaccccctc ggaagaagaa aaatagtcag caagatctaa acaccccaga tactcgccca
     2821 gctggtctgg aagaggctga tcagccaccg ttgcctggag aacaaggaat taacttggat
     2881 aactcaggcc ctaaactgcc agaattttca aaccggccac cagccccgtc gcagaattta
     2941 gtctcaaagg aaacttcaac cacagcactg caggcctctg ttgccagacc agagctggag
     3001 gtaaatgctg ccatagtctc tggacaaagc agtgagccca aagagatagt tgaaaagtcc
     3061 aaaatcccag gccgaagaaa ctcccgaact gaagagccaa ctgtggcctc tgaaagtgtg
     3121 gaaaatggac atcgtaaacg atcttctcga cctgcttcag cctccagctc tactaaagac
     3181 ataaccagtg cggtgcaatc caagcgaaga aaatccaagt agtaatgaac tagtccgtaa
     3241 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga ttgcacagta
     3301 acgcgtactg gtcc
//
