LOCUS       sequence-285270-        6293 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6293)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 6293)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2116
FEATURES             Location/Qualifiers
     source          1..6293
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(4502..4513)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 accgccacca tggttttgga tgaccttcca aacttagaag acatctatac ttccttgtgt
       61 tcatcaacaa tggaagactc agagatggat tttgactctg gactagaaga tgatgacaca
      121 aaaagtgata gtattttgga ggattccaca atttttgtgg ccttcaaagg aaatatagat
      181 gataaagact tcaaatggaa attagatgca atattgaaaa acgtgcccaa tttgttacac
      241 atggagtcca gcaagctaaa agtacagaag gtggagccct ggaacagcgt gcgtgtgaca
      301 ttcaacatcc cccgggaagc agcggagcgg ctacggatcc ttgctcagag caacaaccag
      361 cagcttcggg atttagggat tctctccgtt cagattgaag gggaaggtgc tattaacctg
      421 gctttggctc agaaccgaag ccaagatgtg agaatgaatg gacccatggg agctggaaat
      481 tcagttagga tggaggcggg atttcctatg gcaagtggtc caggaataat aaggatgaac
      541 aaccctgcca ctgttatgat acccccgggt ggaaatgtgt catcttccat gatggcacca
      601 ggccccaatc cagagctgca gcccaggact cctcgccctg cttctcagtc agatgcaatg
      661 gatccactcc tctctgggct ccatatacag cagcaaagtc atccctcagg atctttagct
      721 cccccacatc acccaatgca gcctgtctct gtgaacagac aaatgaaccc agctaatttt
      781 ccccagctgc agcagcagca gcaacaacaa caacagcagc agcagcagca gcagcagcaa
      841 caacagcaac agcagcaaca acagttgcag gcaagacccc cacagcaaca tcagcagcaa
      901 cagccacagg gaattcgacc ccagtttact gccccaactc aggtgcctgt tcctccaggc
      961 tggaaccagc tgccttctgg agcccttcaa cctcctccag cccagggttc tctgggcaca
     1021 atgactgcaa accaagggtg gaagaaggct cccttgcccg gcccaatgca acagcaactc
     1081 caggcaagac catccttagc cacggtacag acgccttccc accctccccc tccatatccc
     1141 tttggcagcc agcaagcctc acaagcccac acaaactttc ctcagatgag caacccaggc
     1201 cagttcacag ctcctcagat gaagagtttg cagggagggc cctctagggt cccaactccc
     1261 ttgcagcagc cccacctcac caacaagtct cctgcctcct caccctcctc cttccagcag
     1321 ggatcccctg catcctcccc aacggttaac caaactcagc agcagatggg accaaggcca
     1381 cctcaaaata acccacttcc ccagggattt cagcagcctg tcagctctcc gggtcggaat
     1441 cctatggttc aacagggaaa tgtgccacct aacttcatgg tgatgcagca gcaaccacca
     1501 aaccaggggc cacagagttt acatccaggc ctaggaggaa tgcctaaacg cctcccacct
     1561 ggcttctcag caggacaggc caatccgaac tttatgcaag gtcaggtgcc ttcgaccaca
     1621 gcaaccaccc ctgggaattc aggagcccct cagctgcaag caaatcaaaa tgtccagcat
     1681 gcaggtggtc aaggagctgg tcctcctcaa aaccagatgc aggtgtccca cgggccgcca
     1741 aatatgatgc agcccagcct catgggaatt catggcaaca tgaacaatca gcaggctggt
     1801 acttctgggg ttcctcaagt gaacctcagc aacatgcaag gccagcccca gcagggccca
     1861 ccatctcagc tgatgggcat gcaccagcaa atcgtgccct cccagggcca gatggtccag
     1921 caacaaggaa ccttgaaccc tcagaaccct atgatccttt caagggccca gcttatgcca
     1981 cagggccaga tgatggtgaa ccccccgagc caaaatcttg ggccctcgcc ccaaaggatg
     2041 accccaccca agcagatgct ttcccagcag ggcccacaaa tgatggcgcc acataaccag
     2101 atgatggggc ctcaggggca ggttttgctc caacagaacc caatgataga gcagattatg
     2161 accaatcaaa tgcaggggaa taagcagcag tttaacactc agaaccagtc caatgtcatg
     2221 ccgggaccag cccagataat gaggggacca actccaaaca tgcaaggaaa tatggtgcag
     2281 tttacgggac agatgtcagg acagatgctg ccccagcaag ggcctgtgaa caacagtcca
     2341 tctcaggtta tgggcattca gggacaggtc ctgcggccac cagggcccag cccacacatg
     2401 gcccagcagc atggtgatcc tgctactaca gcaaataacg atgtcagttt atctcagatg
     2461 atgcctgatg ttagcattca acaaaccaac atggtccccc ctcatgtgca ggccatgcag
     2521 ggaaacagtg cctcgggaaa ccacttctca ggccatggga tgtctttcaa tgcacctttc
     2581 agtggagctc ccaatggaaa tcagatgtcc tgtggtcaaa atccaggctt cccagtcaat
     2641 aaggatgtca cgctaacgag cccattgttg gtcaacttat tgcagagtga catatctgca
     2701 ggccattttg gggtaaacaa taagcaaaat aataccaacg caaataaacc gaagaagaag
     2761 aaaccccctc ggaagaagaa aaatagtcag caagatctaa acaccccaga tactcgccca
     2821 gctggtctgg aagaggctga tcagccaccg ttgcctggag aacaaggaat taacttggat
     2881 aactcaggcc ctaaactgcc agaattttca aaccggccac caggttatcc ttctcaacca
     2941 gttgaacaga ggccacttca gcagatgcct cctcaactca tgcagcatgt ggcaccccca
     3001 ccacagccac cacagcagca gccacagcca caactgcctc agcagcagca gccaccacct
     3061 cccagtcagc cacagtctca gcagcagcag cagcagcagc aacaaatgat gatgatgctc
     3121 atgatgcagc aggatcccaa atcagttagg cttccagtct ctcaaaatgt ccatcctcca
     3181 aggggccccc tgaaccccga ctcccagaga atgcccatgc aacagagtgg cagtgtgcct
     3241 gtcatggtca gtctgcaagg acctgcctcc gtgccaccat cacctgataa acaaagaatg
     3301 ccaatgcctg tgaatactcc cttgggaagc aattcaagga aaatggtcta tcaggagagc
     3361 ccgcagaatc cttccagctc gccactggcg gagatggcct cactccctga agcaagtggc
     3421 agtgaagcac catctgtccc aggaggccca aacaacatgc cttcacatgt agtacttccc
     3481 cagaatcagt taatgatgac agggccaaaa cctggaccat cgcccctttc agcaactcaa
     3541 ggtgcaactc cccagcaacc ccctgtaaat tccctgccca gctctcacgg ccaccacttc
     3601 ccaaatgtgg ctgcgccaac ccagacatct aggcccaaaa caccaaacag agccagcccc
     3661 agaccctatt atcctcagac acccaacaac cgccctccca gcacagaacc ttcagaaatc
     3721 agtctgtcac cagaaagact caatgcctcc atagcaggac tcttccctcc acagattaat
     3781 attcctttac ctcctaggcc aaatttaaac aggggctttg atcaacaagg cctaaatcca
     3841 acaactttga aggccatcgg gcaagcacct tcaaatctta ccatgaatcc ttccaatttt
     3901 gctaccccac aaactcacaa attagattct gtggtagtga attctggaaa gcagtctaat
     3961 tctggagcaa caaaacgggc aagtccaagc aacagtcgca ggtctagtcc tgggtccagt
     4021 aggaaaacca ctccaagccc tgggaggcaa aattcaaaag cccctaaact tactctggcc
     4081 tctcagacaa atgcagccct attgcagaat gtggagttgc cgagaaatgt attggtcagt
     4141 cccactcctc tggccaatcc ccctgtacct gggagctttc ctaacaacag tgggctgaat
     4201 cctcagaatt ctactgtgtc tgtggctgca gttgggggtg ttgttgagga taacaaggag
     4261 agcttgaatg tgcctcagga cagtgattgc cagaattccc agagtaggaa ggaacaggta
     4321 aacattgaac taaaagcagt ccctgcccaa gaagttaaaa tggttgtccc tgaagatcag
     4381 tccaaaaagg atgggcagcc ttcggatcct aacaaacttc ccagtgtcga agagaacaaa
     4441 aatttggtgt ctcctgctat gagggaagca ccaacatcgt taagtcaact tcttgacaac
     4501 tctggagctc ccaatgtgac aattaaaccc cctgggctta cagatctgga agtaacacct
     4561 ccagtagttt ctggggagga cctcaaaaaa gcatctgtca ttcccacact gcaggatctg
     4621 tcttcttcta aagaaccttc taattcccta aacttacctc acagtaatga gctgtgttca
     4681 tcccttgtgc atcccgaatt gagtgaggtc agttctaacg ttgcaccaag catccctcca
     4741 gtaatgtcaa gacctgttag ctcttcctcc atttccactc ccttgccccc aaatcaaata
     4801 actgtatttg tcacttccaa tcccatcaca acttcagcta acacatcagc agctttgcca
     4861 actcacttgc agtctgcatt gatgtcaaca gttgtcacaa tgcccaatgc gggtagcaag
     4921 gttatggttt ctgagggaca gtcagctgct cagtctaatg cccggcctca gttcattaca
     4981 cctgtcttta tcaattcatc ctcaataatt caggttatga aaggatcaca gccaagcaca
     5041 attcctgcag ccccactgac aaccaactct ggcctgatgc ctccctctgt tgcagttgtt
     5101 ggccctttac acatacctca gaacataaaa ttttcttctg ctcctgtacc gcctaatgcc
     5161 ctctccagta gtcctgctcc aaacatccag acaggtcgac ctttggtcct tagctcacga
     5221 gccacccctg ttcagcttcc ttcccctcct tgtacgtctt ctccagttgt cccttctcat
     5281 ccccctgtgc agcaagtgaa agaattgaat ccagatgagg ctagccctca ggtgaacacc
     5341 tcagcagatc agaacactct tccctcttca cagtcaacca caatggtttc tccccttttg
     5401 accaatagtc cagggtcctc tggcaaccgg cgaagcccag tctcgtctag taagggcaaa
     5461 ggaaaagtgg acaaaattgg ccaaattttg ttgaccaagg catgtaagaa agttacaggc
     5521 tctcttgaga aaggggaaga acaatatggt gcagatggag agactgaagg ccaagggcta
     5581 gacaccacag ctccggggct catgggaaca gagcagttat ccacagagct ggacagtaaa
     5641 accccaacgc ccccagcacc cactctgcta aaaatgacct ctagccctgt gggcccgggc
     5701 actgcctcag caggacccag cttacctggc ggtgctctcc ccaccagtgt acgctcgata
     5761 gtaaccactc tggtaccctc cgagctcatc tccgccgtac cgaccacaaa aagcaatcat
     5821 ggtggcatag catctgagtc acttgcgggt ggcctagtgg aggagaaggt gggatcccat
     5881 ccagaacttc tacccagcat agccccgtcg cagaatttag tctcaaagga aacttcaacc
     5941 acagcactgc aggcctctgt tgccagacca gagctggagg taaatgctgc catagtctct
     6001 ggacaaagca gtgagcccaa agagatagtt gaaaagtcca aaatcccagg ccgaagaaac
     6061 tcccgaactg aagagccaac tgtggcctct gaaagtgtgg aaaatggaca tcgtaaacga
     6121 tcttctcgac ctgcttcagc ctccagctct actaaagaca taaccagtgc ggtgcaatcc
     6181 aagcgaagaa aatccaagta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     6241 tggctttata tatcttgtgg aaaggacgac gcagtagagt taaagcctac agc
//
