LOCUS       sequence-285276-        7346 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7346)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 7346)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2509
FEATURES             Location/Qualifiers
     source          1..7346
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3151..5001
                     /codon_start=1
                     /gene="EP300"
                     /product="catalytic histone acetyltransferase core of the 
                     human E1A-associated protein p300 (Delvecchio et al., 
                     2013)"
                     /label=p300 core
                     /translation="IFKPEELRQALMPTLEALYRQDPESLPFRQPVDPQLLGIPDYFDI
                     VKSPMDLSTIKRKLDTGQYQEPWQYVDDIWLMFNNAWLYNRKTSRVYKYCSKLSEVFEQ
                     EIDPVMQSLGYCCGRKLEFSPQTLCCYGKQLCTIPRDATYYSYQNRYHFCEKCFNEIQG
                     ESVSLGDDPSQPQTTINKEQFSKRKNDTLDPELFVECTECGRKMHQICVLHHEIIWPAG
                     FVCDGCLKKSARTRKENKFSAKRLPSTRLGTFLENRVNDFLRRQNHPESGEVTVRVVHA
                     SDKTVEVKPGMKARFVDSGEMAESFPYRTKALFAFEEIDGVDLCFFGMHVQEYGSDCPP
                     PNQRRVYISYLDSVHFFRPKCLRTAVYHEILIGYLEYVKKLGYTTGHIWACPPSEGDDY
                     IFHCHPPDQKIPKPKRLQEWYKKMLDKAVSERIVHDYKDIFKQATEDRLTSAKELPYFE
                     GDFWPNVLEESIKELEQEEEERKREENTSNESTDVTKGDSKNAKKKNNKKTSKNKSSLS
                     RGNKKKPGMPNVSNDLSQKLYATMEKHKEVFFVIRLIAGPAANSLPPIVDPDPLIPCDL
                     MDGRDAFLTLARDKHLEFSSLRRAQWSTMCMLVELHTQSQD"
     regulatory      4765..4774
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggccgagaa tgtggtggaa ccggggccgc cttcagccaa gcggcctaaa
       61 ctctcatctc cggccctctc ggcgtccgcc agcgatggca cagattttgg ctctctattt
      121 gacttggagc acgacttacc agatgaatta atcaactcta cagaattggg actaaccaat
      181 ggtggtgata ttaatcagct tcagacaagt cttggcatgg tacaagatgc agcttctaaa
      241 cataaacagc tgtcagaatt gctgcgatct ggtagttccc ctaacctcaa tatgggagtt
      301 ggtggcccag gtcaagtcat ggccagccag gcccaacaga gcagtcctgg attaggtttg
      361 ataaatagca tggtcaaaag cccaatgaca caggcaggct tgacttctcc caacatgggg
      421 atgggcacta gtggaccaaa tcagggtcct acgcagtcaa caggtatgat gaacagtcca
      481 gtaaatcagc ctgccatggg aatgaacaca gggatgaatg cgggcatgaa tcctggaatg
      541 ttggctgcag gcaatggaca agggataatg cctaatcaag tcatgaacgg ttcaattgga
      601 gcaggccgag ggcgacagaa tatgcagtac ccaaacccag gcatgggaag tgctggcaac
      661 ttactgactg agcctcttca gcagggctct ccccagatgg gaggacaaac aggattgaga
      721 ggcccccagc ctcttaagat gggaatgatg aacaacccca atccttatgg ttcaccatat
      781 actcagaatc ctggacagca gattggagcc agtggccttg gtctccagat tcagacaaaa
      841 actgtactat caaataactt atctccattt gctatggaca aaaaggcagt tcctggtgga
      901 ggaatgccca acatgggtca acagccagcc ccgcaggtcc agcagccagg cctggtgact
      961 ccagttgccc aagggatggg ttctggagca catacagctg atccagagaa gcgcaagctc
     1021 atccagcagc agcttgttct ccttttgcat gctcacaagt gccagcgccg ggaacaggcc
     1081 aatggggaag tgaggcagtg caaccttccc cactgtcgca caatgaagaa tgtcctaaac
     1141 cacatgacac actgccagtc aggcaagtct tgccaagtgg cacactgtgc atcttctcga
     1201 caaatcattt cacactggaa gaattgtaca agacatgatt gtcctgtgtg tctccccctc
     1261 aaaaatgctg gtgataagag aaatcaacag ccaattttga ctggagcacc cgttggactt
     1321 ggaaatccta gctctctagg ggtgggtcaa cagtctgccc ccaacctaag cactgttagt
     1381 cagattgatc ccagctccat agaaagagcc tatgcagctc ttggactacc ctatcaagta
     1441 aatcagatgc cgacacaacc ccaggtgcaa gcaaagaacc agcagaatca gcagcctggg
     1501 cagtctcccc aaggcatgcg gcccatgagc aacatgagtg ctagtcctat gggagtaaat
     1561 ggaggtgtag gagttcaaac gccgagtctt ctttctgact caatgttgca ttcagccata
     1621 aattctcaaa acccaatgat gagtgaaaat gccagtgtgc cctccctggg tcctatgcca
     1681 acagcagctc aaccatccac tactggaatt cggaaacagt ggcacgaaga tattactcag
     1741 gatcttcgaa atcatcttgt tcacaaactc gtccaagcca tatttcctac gccggatcct
     1801 gctgctttaa aagacagacg gatggaaaac ctagttgcat atgctcggaa agttgaaggg
     1861 gacatgtatg aatctgcaaa caatcgagcg gaatactacc accttctagc tgagaaaatc
     1921 tataagatcc agaaagaact agaagaaaaa cgaaggacca gactacagaa gcagaacatg
     1981 ctaccaaatg ctgcaggcat ggttccagtt tccatgaatc cagggcctaa catgggacag
     2041 ccgcaaccag gaatgacttc taatggccct ctacctgacc caagtatgat ccgtggcagt
     2101 gtgccaaacc agatgatgcc tcgaataact ccacaatctg gtttgaatca atttggccag
     2161 atgagcatgg cccagccccc tattgtaccc cggcaaaccc ctcctcttca gcaccatgga
     2221 cagttggctc aacctggagc tctcaacccg cctatgggct atgggcctcg tatgcaacag
     2281 ccttccaacc agggccagtt ccttcctcag actcagttcc catcacaggg aatgaatgta
     2341 acaaatatcc ctttggctcc gtccagcggt caagctccag tgtctcaagc acaaatgtct
     2401 agttcttcct gcccggtgaa ctctcctata atgcctccag ggtctcaggg gagccacatt
     2461 cactgtcccc agcttcctca accagctctt catcagaatt caccctcgcc tgtacctagt
     2521 cgtaccccca cccctcacca tactccccca agcatagggg ctcagcagcc accagcaaca
     2581 acaattccag cccctgttcc tacacctcct gccatgccac ctgggccaca gtcccaggct
     2641 ctacatcccc ctccaaggca gacacctaca ccaccaacaa cacaacttcc ccaacaagtg
     2701 cagccttcac ttcctgctgc accttctgct gaccagcccc agcagcagcc tcgctcacag
     2761 cagagcacag cagcgtctgt tcctacccca acagcaccgc tgcttcctcc gcagcctgca
     2821 actccacttt cccagccagc tgtaagcatt gaaggacagg tatcaaatcc tccatctact
     2881 agtagcacag aagtgaattc tcaggccatt gctgagaagc agccttccca ggaagtgaag
     2941 atggaggcca aaatggaagt ggatcaacca gaaccagcag atactcagcc ggaggatatt
     3001 tcagagtcta aagtggaaga ctgtaaaatg gaatctaccg aaacagaaga gagaagcact
     3061 gagttaaaaa ctgaaataaa agaggaggaa gaccagccaa gtacttcagc tacccagtca
     3121 tctccggctc caggacagtc aaagaaaaag attttcaaac cagaagaact acgacaggca
     3181 ctgatgccaa ctttggaggc actttaccgt caggatccag aatcccttcc ctttcgtcaa
     3241 cctgtggacc ctcagctttt aggaatccct gattactttg atattgtgaa gagccccatg
     3301 gatctttcta ccattaagag gaagttagac actggacagt atcaggagcc ctggcagtat
     3361 gtcgatgata tttggcttat gttcaataat gcctggttat ataaccggaa aacatcacgg
     3421 gtatacaaat actgctccaa gctctctgag gtctttgaac aagaaattga cccagtgatg
     3481 caaagccttg gatactgttg tggcagaaag ttggagttct ctccacagac actgtgttgc
     3541 tacggcaaac agttgtgcac aatacctcgt gatgccactt attacagtta ccagaacagg
     3601 tatcatttct gtgagaagtg tttcaatgag atccaagggg agagcgtttc tttgggggat
     3661 gacccttccc agcctcaaac tacaataaat aaagaacaat tttccaagag aaaaaatgac
     3721 acactggatc ctgaactgtt tgttgaatgt acagagtgcg gaagaaagat gcatcagatc
     3781 tgtgtccttc accatgagat catctggcct gctggattcg tctgtgatgg ctgtttaaag
     3841 aaaagtgcac gaactaggaa agaaaataag ttttctgcta aaaggttgcc atctaccaga
     3901 cttggcacct ttctagagaa tcgtgtgaat gactttctga ggcgacagaa tcaccctgag
     3961 tcaggagagg tcactgttag agtagttcat gcttctgaca aaaccgtgga agtaaaacca
     4021 ggcatgaaag caaggtttgt ggacagtgga gagatggcag aatcctttcc ataccgaacc
     4081 aaagccctct ttgcctttga agaaattgat ggtgttgacc tgtgcttctt tggcatgcat
     4141 gttcaagagt atggctctga ctgccctcca cccaaccaga ggagagtata catatcttac
     4201 ctcgatagtg ttcatttctt ccgtcctaaa tgcttgagga ctgcagtcta tcatgaaatc
     4261 ctaattggat atttagaata tgtcaagaaa ttaggttaca caacagggca tatttgggca
     4321 tgtccaccaa gtgagggaga tgattatatc ttccattgcc atcctcctga ccagaagata
     4381 cccaagccca agcgactgca ggaatggtac aaaaaaatgc ttgacaaggc tgtatcagag
     4441 cgtattgtcc atgactacaa ggatattttt aaacaagcta ctgaagatag attaacaagt
     4501 gcaaaggaat tgccttattt cgagggtgat ttctggccca atgttctgga agaaagcatt
     4561 aaggaactgg aacaggagga agaagagaga aaacgagagg aaaacaccag caatgaaagc
     4621 acagatgtga ccaagggaga cagcaaaaat gctaaaaaga agaataataa gaaaaccagc
     4681 aaaaataaga gcagcctgag taggggcaac aagaagaaac ccgggatgcc caatgtatct
     4741 aacgacctct cacagaaact atatgccacc atggagaagc ataaagaggt cttctttgtg
     4801 atccgcctca ttgctggccc tgctgccaac tccctgcctc ccattgttga tcctgatcct
     4861 ctcatcccct gcgatctgat ggatggtcgg gatgcgtttc tcacgctggc aagggacaag
     4921 cacctggagt tctcttcact ccgaagagcc cagtggtcca ccatgtgcat gctggtggag
     4981 ctgcacacgc agagccagga ccgctttgtc tacacctgca atgaatgcaa gcaccatgtg
     5041 gagacacgct ggcactgtac tgtctgtgag gattatgact tgtgtatcac ctgctataac
     5101 actaaaaacc atgaccacaa aatggagaaa ctaggccttg gcttagatga tgagagcaac
     5161 aaccagcagg ctgcagccac ccagagccca ggcgattctc gccgcctgag tatccagcgc
     5221 tgcatccagt ctctggtcca tgcttgccag tgtcggaatg ccaattgctc actgccatcc
     5281 tgccagaaga tgaagcgggt tgtgcagcat accaagggtt gcaaacggaa aaccaatggc
     5341 gggtgcccca tctgcaagca gctcattgcc ctctgctgct accatgccaa gcactgccag
     5401 gagaacaaat gcccggtgcc gttctgccta aacatcaagc agaagctccg gcagcaacag
     5461 ctgcagcacc gactacagca ggcccaaatg cttcgcagga ggatggccag catgcagcgg
     5521 actggtgtgg ttgggcagca acagggcctc ccttccccca ctcctgccac tccaacgaca
     5581 ccaactggcc aacagccaac caccccgcag acgccccagc ccacttctca gcctcagcct
     5641 acccctccca atagcatgcc accctacttg cccaggactc aagctgctgg ccctgtgtcc
     5701 cagggtaagg cagcaggcca ggtgacccct ccaacccctc ctcagactgc tcagccaccc
     5761 cttccagggc ccccacctgc agcagtggaa atggcaatgc agattcagag agcagcggag
     5821 acgcagcgcc agatggccca cgtgcaaatt tttcaaaggc caatccaaca ccagatgccc
     5881 ccgatgactc ccatggcccc catgggtatg aacccacctc ccatgaccag aggtcccagt
     5941 gggcatttgg agccagggat gggaccgaca gggatgcagc aacagccacc ctggagccaa
     6001 ggaggattgc ctcagcccca gcaactacag tctgggatgc caaggccagc catgatgtca
     6061 gtggcccagc atggtcaacc tttgaacatg gctccacaac caggattggg ccaggtaggt
     6121 atcagcccac tcaaaccagg cactgtgtct caacaagcct tacaaaacct tttgcggact
     6181 ctcaggtctc ccagctctcc cctgcagcag caacaggtgc ttagtatcct tcacgccaac
     6241 ccccagctgt tggctgcatt catcaagcag cgggctgcca agtatgccaa ctctaatcca
     6301 caacccatcc ctgggcagcc tggcatgccc caggggcagc cagggctaca gccacctacc
     6361 atgccaggtc agcagggggt ccactccaat ccagccatgc agaacatgaa tccaatgcag
     6421 gcgggcgttc agagggctgg cctgccccag cagcaaccac agcagcaact ccagccaccc
     6481 atgggaggga tgagccccca ggctcagcag atgaacatga accacaacac catgccttca
     6541 caattccgag acatcttgag acgacagcaa atgatgcaac agcagcagca acagggagca
     6601 gggccaggaa taggccctgg aatggccaac cataaccagt tccagcaacc ccaaggagtt
     6661 ggctacccac cacagcagca gcagcggatg cagcatcaca tgcaacagat gcaacaagga
     6721 aatatgggac agataggcca gcttccccag gccttgggag cagaggcagg tgccagtcta
     6781 caggcctatc agcagcgact ccttcagcaa cagatggggt cccctgttca gcccaacccc
     6841 atgagccccc agcagcatat gctcccaaat caggcccagt ccccacacct acaaggccag
     6901 cagatcccta attctctctc caatcaagtg cgctctcccc agcctgtccc ttctccacgg
     6961 ccacagtccc agccccccca ctccagtcct tccccaagga tgcagcctca gccttctcca
     7021 caccacgttt ccccacagac aagttcccca catcctggac tggtagctgc ccaggccaac
     7081 cccatggaac aagggcattt tgccagcccg gaccagaatt caatgctttc tcagcttgct
     7141 agcaatccag gcatggcaaa cctccatggt gcaagcgcca cggacctggg actcagcacc
     7201 gataactcag acttgaattc aaacctctca cagagtacac tagacataca ctagtaatga
     7261 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     7321 gagatgtcaa gatggcccga tagcgc
//
