LOCUS       sequence-285284-       11752 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11752)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2566
FEATURES             Location/Qualifiers
     source          1..11752
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    7609..8197
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7662..7682)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8080..8091)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8269..8502
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8580..8701
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8617..8636)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8671..8690
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8741..8876
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8896..8915)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8897..8915)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8925..8942)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8925..8941)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8934..8956)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9083..9538
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9170..9189)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9380..9401
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9564..9668
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9669..9737,9738..10529)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9887..9906)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10700..11288
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11189..11208
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11442..11459
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11576..11597
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11612..11617,11618..11635,11636..11642)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11650..11666
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11655..11677
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11674..11690
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11674..11690
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11709..11729
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11711..11729
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggcg gaggaagaga gcgaccaaga
     4141 ggccgaacgc ctcggagaag agcttgtggc cattgtggag tccccgctgg gccctgtggg
     4201 gcttagagct gcgggcgacg gcagaggcgg cgctggcagc ggcaactgcg gcggcggcgt
     4261 cggaatcagc agtcgggatt actgccgacg cttctgtcag gtggttgaag attatgctgg
     4321 aagatggcag gtccctttgc cacagcttca ggttcttcag actgcccttt gttgttttac
     4381 aacagccagt gcatcattcc cagatgaatg tgagcatgta caatatgttt tgagtagcct
     4441 tgctgtgagt ttctttgagt tgctgctgtt ctttggaaga gatgagtttt atgaagagcc
     4501 cttaaaggat attcttggat cattccagga atgccagaat cacctccgca gatatggaaa
     4561 tgtgaatctg gaactggtga ctcgaatcat tagagatggt ggcccatggg aagatccagt
     4621 gttgcaagct gtccttaaag ctcagccagc atctcaggag atagtgaaca aatatttaag
     4681 ttctgaaaat ccactgttct ttgaactacg tgccagatac ctaattgctt gtgaacgcat
     4741 acccgaagca atggctctta ttaaatcttg tataaatcac ccagaaatca gtaaagactt
     4801 atacttccat caagcactct tcacatgtct gtttatgtca cctgtagaag atcagctatt
     4861 ccgggagcat ttattgaaaa ctgattgtaa gagtggaatt gatatcatct gtaatgctga
     4921 aaaagaaggc aaaactatgt tagccttgca actctgtgaa tcctttctta ttccacagct
     4981 ccagaatggg gatatgtact gtatctggga gttgattttc atatggagta aactacagct
     5041 taaatctaat ccttcaaaac aagtttttgt agatcaatgc taccagcttt taagaacagc
     5101 aactaatgtg agagtcatat ttcctttcat gaaaatcatc aaagatgagg ttgaagaaga
     5161 aggcttgcaa atttgtgttg aaatatgtgg ttgtgctcta caactcgacc ttcatgatga
     5221 tcccaaaact aaatgtctaa tttataaaac aattgcacat tttttgccaa atgatttgga
     5281 gatcctcagg atttgtgcac tctcaatatt ttttctggag cgctccttag aagcgtatcg
     5341 tactgttgaa gagctttaca aacgtccaga tgaagaatat aatgaaggca caagtagtgt
     5401 tcaaaatcgt gttcgttttg aattgcttcc aattttgaaa aagggattgt tttttgaccc
     5461 tgaattttgg aacttcgtaa tgattaagaa aaactgtgta gcattattga gtgataaatc
     5521 agcagttaga tttctaaatg aaagcacact ggaaaataat gcaggtaatc taaaaaggac
     5581 ggaggaacag caaggtttgg atgaagggtt tgactctctt acagatcaga gcactggaga
     5641 gactgatcct gatgatgtat ctggagtgca gcctaaaggt catattaata cgaagaaaaa
     5701 tcttacagct ctcagtactt ccaaagtaga tcacaatgtc ccaaggcatc gttgtatgtt
     5761 atgtaacaag gaatttttag gtggtcacat tgtaaggcat gcccaggctc atcagaaaaa
     5821 aggcagtttt gcatgtgtaa tatgtggtag gaaatttaga aacagaggac ttatgcagaa
     5881 gcatttgaag aatcatgtta agaagataca gaggcagcaa attgctgcag ctcaacagga
     5941 tgatcaggaa gtcactgctt tggaagaaat aaattgttct agttcttcca tttcatttga
     6001 aaatgggaat tctgatagta aggatttgga agtggagaca cttactgctt ctagtgaagg
     6061 aaacaaagaa gtcatccctg agcatgtggc tgaattcatt gaaattccca taagtgtacc
     6121 agaagatgtt attgaaaatg ttattgaaaa tggcagtcct aataattctt taaataatgt
     6181 tttcaagcct ttaactgaat gtggggatga ttatgaggag gaagaggatg aagaaggtga
     6241 ttatgaagaa gatgattatg acctgaatca agaaacttca gtaattcata aaatcaatgg
     6301 aactgtgtgc catccaaaag acatatatgc cacagatcaa gaaggaaact ttaagtgtcc
     6361 tgctcttggt tgtgtccgga tatttaaaag aattgggttt ctaaataaac atgcaatgac
     6421 cgtacatcca accgatttaa atgtgcgaca aacagtaatg aagtggagca aaggaaaatg
     6481 caaattttgt caaaggcaat ttgaagattc tcaacatttt atagaccacc ttaatagaca
     6541 tagctatcca aatgtgtatt tttgtttgca ttttaattgc aacgagtcgt ttaagctgcc
     6601 gttccagctt gcccagcaca caaaaagtca caggatattt caggctcagt gtagttttcc
     6661 agaatgccat gagctttttg aagatcttcc tctgctgtat gaacatgaag ctcaacacta
     6721 tttaagtaaa acaccagagt catctgcaca accaagtgaa acaattcttt gggatgttca
     6781 gacagactca aatcctaatc aggaaaaaga ctcatctagt aatgagaaac aaactattag
     6841 tctgccagtt tctactagca aatcaaggaa agagtctaca gaaccaaaga catgtataga
     6901 aagtatggaa aagaaaacag acagtttagt tcagaatgga aacgaacgtt ctgatgacac
     6961 tgtttcaaat ataagcttga tagaccaaaa gatgcctgac atagagccaa attctgaaaa
     7021 taattgtagt agtagtgata tagtcaatgg acacagtgaa atagagcaaa cacctttagt
     7081 ttcatcagat cctgctttga aaattgatac aaacagaatc aggacagaaa atggttccat
     7141 tttgcccagt gttgtaccac aagaacacaa caccttgcca gtatctcagg caccttccaa
     7201 accaaatctg acaagtgaac atacttcata tggcttaatt ttaacaaaac catacgtcag
     7261 accattgcct cccagttacc ttgatgaacg gtatcttagt atgccaaaac gcagaaaatt
     7321 tctgactgat agagtagatg cctgttctga tcaagataac gtgtataaaa aatcagtgaa
     7381 aagattaaga tgtggcaaat gcctgaccac ctactgtaat gcagaagcac ttgaggctca
     7441 tcttgcacaa aagaaatgtc agacactctt tggatttgat tcagatgatg aaagtgccta
     7501 gtaatgaact agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg
     7561 aaaggacgag gaaacatgcc ggtttagaac gctacgcgtt aagtcgacaa tcaacctctg
     7621 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     7681 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     7741 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     7801 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     7861 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     7921 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     7981 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     8041 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     8101 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     8161 cagacgagtc ggatctccct ttgggccgcc tccccgcgtc gactttaaga ccaatgactt
     8221 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     8281 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     8341 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     8401 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     8461 gatccctcag acccttttag tcagtgtgga aaatctctag cagtacgtat agtagttcat
     8521 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga
     8581 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     8641 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     8701 atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc
     8761 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     8821 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     8881 gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta
     8941 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     9001 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     9061 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     9121 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     9181 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     9241 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     9301 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     9361 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     9421 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     9481 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag
     9541 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     9601 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     9661 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     9721 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     9781 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     9841 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     9901 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     9961 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
    10021 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
    10081 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
    10141 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
    10201 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
    10261 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
    10321 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
    10381 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
    10441 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
    10501 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
    10561 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
    10621 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
    10681 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
    10741 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
    10801 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
    10861 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
    10921 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
    10981 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
    11041 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
    11101 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
    11161 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
    11221 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    11281 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
    11341 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
    11401 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
    11461 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
    11521 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
    11581 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
    11641 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
    11701 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa gc
//
