LOCUS       sequence-285285-        3488 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3488)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2566
FEATURES             Location/Qualifiers
     source          1..3488
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggagga agagagcgac caagaggccg aacgcctcgg agaagagctt
       61 gtggccattg tggagtcccc gctgggccct gtggggctta gagctgcggg cgacggcaga
      121 ggcggcgctg gcagcggcaa ctgcggcggc ggcgtcggaa tcagcagtcg ggattactgc
      181 cgacgcttct gtcaggtggt tgaagattat gctggaagat ggcaggtccc tttgccacag
      241 cttcaggttc ttcagactgc cctttgttgt tttacaacag ccagtgcatc attcccagat
      301 gaatgtgagc atgtacaata tgttttgagt agccttgctg tgagtttctt tgagttgctg
      361 ctgttctttg gaagagatga gttttatgaa gagcccttaa aggatattct tggatcattc
      421 caggaatgcc agaatcacct ccgcagatat ggaaatgtga atctggaact ggtgactcga
      481 atcattagag atggtggccc atgggaagat ccagtgttgc aagctgtcct taaagctcag
      541 ccagcatctc aggagatagt gaacaaatat ttaagttctg aaaatccact gttctttgaa
      601 ctacgtgcca gatacctaat tgcttgtgaa cgcatacccg aagcaatggc tcttattaaa
      661 tcttgtataa atcacccaga aatcagtaaa gacttatact tccatcaagc actcttcaca
      721 tgtctgttta tgtcacctgt agaagatcag ctattccggg agcatttatt gaaaactgat
      781 tgtaagagtg gaattgatat catctgtaat gctgaaaaag aaggcaaaac tatgttagcc
      841 ttgcaactct gtgaatcctt tcttattcca cagctccaga atggggatat gtactgtatc
      901 tgggagttga ttttcatatg gagtaaacta cagcttaaat ctaatccttc aaaacaagtt
      961 tttgtagatc aatgctacca gcttttaaga acagcaacta atgtgagagt catatttcct
     1021 ttcatgaaaa tcatcaaaga tgaggttgaa gaagaaggct tgcaaatttg tgttgaaata
     1081 tgtggttgtg ctctacaact cgaccttcat gatgatccca aaactaaatg tctaatttat
     1141 aaaacaattg cacatttttt gccaaatgat ttggagatcc tcaggatttg tgcactctca
     1201 atattttttc tggagcgctc cttagaagcg tatcgtactg ttgaagagct ttacaaacgt
     1261 ccagatgaag aatataatga aggcacaagt agtgttcaaa atcgtgttcg ttttgaattg
     1321 cttccaattt tgaaaaaggg attgtttttt gaccctgaat tttggaactt cgtaatgatt
     1381 aagaaaaact gtgtagcatt attgagtgat aaatcagcag ttagatttct aaatgaaagc
     1441 acactggaaa ataatgcagg taatctaaaa aggacggagg aacagcaagg tttggatgaa
     1501 gggtttgact ctcttacaga tcagagcact ggagagactg atcctgatga tgtatctgga
     1561 gtgcagccta aaggtcatat taatacgaag aaaaatctta cagctctcag tacttccaaa
     1621 gtagatcaca atgtcccaag gcatcgttgt atgttatgta acaaggaatt tttaggtggt
     1681 cacattgtaa ggcatgccca ggctcatcag aaaaaaggca gttttgcatg tgtaatatgt
     1741 ggtaggaaat ttagaaacag aggacttatg cagaagcatt tgaagaatca tgttaagaag
     1801 atacagaggc agcaaattgc tgcagctcaa caggatgatc aggaagtcac tgctttggaa
     1861 gaaataaatt gttctagttc ttccatttca tttgaaaatg ggaattctga tagtaaggat
     1921 ttggaagtgg agacacttac tgcttctagt gaaggaaaca aagaagtcat ccctgagcat
     1981 gtggctgaat tcattgaaat tcccataagt gtaccagaag atgttattga aaatgttatt
     2041 gaaaatggca gtcctaataa ttctttaaat aatgttttca agcctttaac tgaatgtggg
     2101 gatgattatg aggaggaaga ggatgaagaa ggtgattatg aagaagatga ttatgacctg
     2161 aatcaagaaa cttcagtaat tcataaaatc aatggaactg tgtgccatcc aaaagacata
     2221 tatgccacag atcaagaagg aaactttaag tgtcctgctc ttggttgtgt ccggatattt
     2281 aaaagaattg ggtttctaaa taaacatgca atgaccgtac atccaaccga tttaaatgtg
     2341 cgacaaacag taatgaagtg gagcaaagga aaatgcaaat tttgtcaaag gcaatttgaa
     2401 gattctcaac attttataga ccaccttaat agacatagct atccaaatgt gtatttttgt
     2461 ttgcatttta attgcaacga gtcgtttaag ctgccgttcc agcttgccca gcacacaaaa
     2521 agtcacagga tatttcaggc tcagtgtagt tttccagaat gccatgagct ttttgaagat
     2581 cttcctctgc tgtatgaaca tgaagctcaa cactatttaa gtaaaacacc agagtcatct
     2641 gcacaaccaa gtgaaacaat tctttgggat gttcagacag actcaaatcc taatcaggaa
     2701 aaagactcat ctagtaatga gaaacaaact attagtctgc cagtttctac tagcaaatca
     2761 aggaaagagt ctacagaacc aaagacatgt atagaaagta tggaaaagaa aacagacagt
     2821 ttagttcaga atggaaacga acgttctgat gacactgttt caaatataag cttgatagac
     2881 caaaagatgc ctgacataga gccaaattct gaaaataatt gtagtagtag tgatatagtc
     2941 aatggacaca gtgaaataga gcaaacacct ttagtttcat cagatcctgc tttgaaaatt
     3001 gatacaaaca gaatcaggac agaaaatggt tccattttgc ccagtgttgt accacaagaa
     3061 cacaacacct tgccagtatc tcaggcacct tccaaaccaa atctgacaag tgaacatact
     3121 tcatatggct taattttaac aaaaccatac gtcagaccat tgcctcccag ttaccttgat
     3181 gaacggtatc ttagtatgcc aaaacgcaga aaatttctga ctgatagagt agatgcctgt
     3241 tctgatcaag ataacgtgta taaaaaatca gtgaaaagat taagatgtgg caaatgcctg
     3301 accacctact gtaatgcaga agcacttgag gctcatcttg cacaaaagaa atgtcagaca
     3361 ctctttggat ttgattcaga tgatgaaagt gcctagtaat gaactagtcc gtaacttgaa
     3421 agtatttcga tttcttggct ttatatatct tgtggaaagg acgaggaaac atgccggttt
     3481 agaacgct
//
