LOCUS       sequence-285293-       13186 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13186)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 13186)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2639
FEATURES             Location/Qualifiers
     source          1..13186
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      6119..6128
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(7156..7173)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     misc_feature    9043..9631
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9096..9116)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9514..9525)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9703..9936
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10014..10135
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10051..10070)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10105..10124
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10175..10310
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10330..10349)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10331..10349)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10359..10376)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10359..10375)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10368..10390)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10517..10972
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10604..10623)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10814..10835
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10998..11102
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11103..11171,11172..11963)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11321..11340)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12134..12722
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12623..12642
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12876..12893
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    13010..13031
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13046..13051,13052..13069,13070..13076)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13084..13100
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13089..13111
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13108..13124
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13108..13124
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     13143..13163
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        13145..13163
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgtat tcggcccaca ggcccctgat
     4141 gcccgcgtcc agcgcggcct cccgtggcct cggcatgttc gtgtggacga atgtggaacc
     4201 tcgctctgtg gctgtgttcc cttggcactc cttagtcccc ttcctggcac ccagccagcc
     4261 tgacccctcc gtgcagccga gcgaggccca gcaacctgcc agccacccag tggcctccaa
     4321 ccagagcaaa gaacctgctg agtcggcagc tgttgctcat gaacggccac caggtgggac
     4381 agggagtgct gaccctgagc ggccccctgg agccacatgc cctgagagcc caggacccgg
     4441 acccccacac cctttggggg tggtggaatc tggtaagggt ccgcctccca ccacggagga
     4501 ggaggcctcc ggccccccag gagagccccg gctggacagt gagacagaga gtgaccatga
     4561 tgatgccttc ctctccatca tgtctcctga gatccagttg cctctaccgc ccggaaaacg
     4621 tcggacccag tccctcagtg ccctacccaa ggaacgggac tcatcttctg agaaggatgg
     4681 acgcagcccc aacaagcggg agaaggacca catccggcgg cccatgaatg ccttcatgat
     4741 cttcagcaag cggcaccggg ccctggtcca ccagcgtcat cccaaccagg acaaccggac
     4801 cgtcagcaag atcctgggcg agtggtggta tgccctgggg cccaaggaga agcagaagta
     4861 ccacgacctg gccttccagg tgaaggaggc ccacttcaag gcccacccag attggaagtg
     4921 gtgcaacaag gaccgaaaga agtccagctc agaggccaag cccacgagcc tggggctggc
     4981 aggagggcac aaggagacgc gggagcggag catgtcggag acgggcactg ctgctgcccc
     5041 tggggtgtcc tctgagctcc tgtccgttgc agcccagaca ctcctgagct cagacaccaa
     5101 ggctccgggg agcagctcct gtggggcaga acggctacac acagttgggg gacctggctc
     5161 agcccggccc cgagctttct cccacagcgg ggtacacagc ctggacggcg gagaagtaga
     5221 cagtcaggcg ctacaggaac tgacgcagat ggtgtctggc cctgcatcgt actctggccc
     5281 aaagccttct acccagtatg gagctccagg accctttgca gcccctggtg agggaggtgc
     5341 cttggcggcc actgggcggc ccccgctgct gcccacccga gcttctcgtt ctcagcgtgc
     5401 ggccagtgag gacatgacga gtgatgagga gcgcatggtc atctgtgagg aggaagggga
     5461 tgatgatgtc attgctgacg atggcttcgg caccactgac attgatctca agtgcaagga
     5521 gcgggtgacc gacagcgaga gtggggacag ctctggggag gacccagagg gcaacaaggg
     5581 ctttggtcgg aaggtgtttt cacctgtgat ccgttcctcc tttacccact gccgcccccc
     5641 actggaccct gagcccccag ggcccccgga tcctcctgta gcctttggca aaggctatgg
     5701 ttccgcccca tcctcctctg cgtcctcgcc tgcttcctcc tcagcctcgg cagccacctc
     5761 cttctcactg ggctcaggaa ccttcaaggc ccaggagtct ggtcagggca gcacagcggg
     5821 ccccctacgg cccccacccc ctggggctgg gggtccagcg acaccttcca aggcaacccg
     5881 gttcctccca atggatcctg ccaccttccg gcgcaagaga cccgaaagtg tgggtggcct
     5941 ggagccacca ggcccctcag tcatcgcggc ccctcccagc ggaggaggaa acatcctgca
     6001 gacactggtg ctgcccccaa acaaggagga gcaagagggc ggcggagcca gagtgccctc
     6061 cgcccccgcc ccatcactgg cctatggggc cccagcagct cccctgtccc gtcctgccgc
     6121 caccatggtc accaatgtgg tgcggcctgt cagcagcact cctgtgccca tcgcctctaa
     6181 gcccttcccc acctctggcc gggctgaggc gtctccaaat gacacagcag gtgccaggac
     6241 tgaaatgggc actgggtctc gggtgcctgg gggctccccg ctgggtgtca gcttagtgta
     6301 ttcggacaag aagtcggcag cagccacctc accagcccca cacttggtgg ctggacccct
     6361 gctgggcact gtggggaagg cgcctgccac tgtcactaac ctactggtgg gcaccccggg
     6421 gtatggggcc cctgcgcccc ctgctgtcca gttcattgcc cagggggccc ctggtggtgg
     6481 gaccactgcg ggctcaggag caggtgctgg gagtggcccc aatgggccag tacccctggg
     6541 catcctgcaa ccaggtgccc tgggcaaggc tgggggaatc acccaggtac agtacatcct
     6601 gcccacgctg ccccagcagc ttcaggtggc acctgcccca gcaccagccc ctgggaccaa
     6661 ggcagcggct cccagcggcc ctgcacccac caccagcatc cgtttcaccc tcccaccggg
     6721 cacttccacc aacggcaaag tcctggctgc cactgcaccc actcctggca tccccatcct
     6781 gcagtctgta ccctccgccc caccccccaa agcccagtca gtttctcccg tgcaggcccc
     6841 gcccccgggt ggctcagccc agctgctgcc tgggaaggtc ctagtgcctc tggccgcccc
     6901 tagcatgtca gtgcggggtg gaggggccgg ccagccactg ccactggtga gcccgccctt
     6961 ctcagtacct gtgcagaatg gtgcccagcc ccccagcaag atcatccagc tgaccccggt
     7021 gcctgtgagc acacccagcg gcctggtgcc gcccctgagc ccagccacac tccctggacc
     7081 cacctctcag cctcagaagg tcctgttgcc ctcctccacc agaatcacct atgtgcagtc
     7141 agcgggcggg cacgcgctgc ccctgggtac cagccctgcg tccagccagg ctggaacagt
     7201 cacctcgtac gggcccacga gctctgtagc tctaggcttc acctcgctgg ggcccagcgg
     7261 ccccgccttc gtgcagcccc tgctctcagg ccaagcccca ctgctggctc ccggtcaggt
     7321 gggcgtgtca cctgtgccca gtccccagct gccgcctgcc tgtgcagccc ccggaggtcc
     7381 tgtcataaca gcattttact ctggcagccc tgcacccacc tcctcagcac ccctggccca
     7441 gccatcccag gcccccccaa gcctggtcta cactgtggcc accagcacaa ccccacctgc
     7501 agccaccatt ctgcccaagg gcccgccagc ccctgccact gccaccccag ccccgactag
     7561 ccctttcccc agcgccacag gttccatgac ctacagctta gtggccccca aggcccagcg
     7621 gcccagcccg aaggcccccc agaaagtgaa ggcagccatc gccagcattc ccgtggggtc
     7681 ctttgaggca ggtgcctctg ggcggcctgg ccctgcaccc cggcagcctc tggagcctgg
     7741 cccagtccga gagccaactg ccccagagtc tgagcttgag gggcagccca caccaccagc
     7801 ccctccaccc ctgccagaga cctggactcc cacggcccgg agcagccccc cactgccccc
     7861 acctgctgag gagcggacca gcgccaaggg ccctgagacc atggccagca aattccccag
     7921 ctcatcttca gactggcgcg tccctgggca gggcctggag aatcgtgggg agcctcccac
     7981 tcctcccagc ccggccccag ctccagctgt agcccctggt ggcagcagcg agagcagcag
     8041 tgggcgggca gccggggaca ccccggagcg caaggaggcg gctggtactg gcaagaaggt
     8101 gaaggtgcgg cccccgcccc tgaagaagac ctttgactct gtggacaaca gggtcctgtc
     8161 agaagtggac ttcgaagagc gctttgctga gttgcctgag tttcggcctg aggaggtgct
     8221 gccctccccc accctgcagt ctctggccac ctcaccccgg gccatcctgg gctcttaccg
     8281 caagaagagg aagaactcca cggacctgga ttcagcaccc gaggacccca cctcgcccaa
     8341 gcgcaagatg agaagacgct ccagctgcag ctcggagccc aacaccccca agagtgccaa
     8401 gtgcgagggg gacatcttca cctttgaccg tacaggtaca gaagccgagg acgtgcttgg
     8461 ggagctagag tatgacaagg tgccatactc ctccctgcgg cgcaccctgg accagcgccg
     8521 ggccctggtc atgcagctct ttcaggacca tggcttcttc ccgtcagccc aggccacagc
     8581 cgccttccag gcccgctatg cagacatctt tccctccaag gtttgtctgc agttgaagat
     8641 ccgtgaggtg cgccagaaga tcatgcaggc tgccactccc acggagcagc cccctggagc
     8701 tgaggctcct ctccctgtac cgccccccac tggcaccgct gctgcccctg cccccactcc
     8761 cagccccgca gggggccctg accccacctc acccagctcg gactctggca cggcccaggc
     8821 tgccccgcca ctgcctccac ccccagagtc ggggcctgga cagcctggct gggagggggc
     8881 tccccagccc tcccccccac ccccaggtcc ctccacagct gccacaggca ggtagtaatg
     8941 aactagtccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
     9001 cgatttctgt tcgctgtagg tcaagcaacg cgttaagtcg acaatcaacc tctggattac
     9061 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     9121 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     9181 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     9241 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     9301 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     9361 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     9421 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     9481 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     9541 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     9601 agtcggatct ccctttgggc cgcctccccg cgtcgacttt aagaccaatg acttacaagg
     9661 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     9721 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     9781 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     9841 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     9901 tcagaccctt ttagtcagtg tggaaaatct ctagcagtac gtatagtagt tcatgtcatc
     9961 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
    10021 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
    10081 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
    10141 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
    10201 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
    10261 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta
    10321 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
    10381 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
    10441 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
    10501 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
    10561 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
    10621 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
    10681 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
    10741 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
    10801 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
    10861 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
    10921 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
    10981 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
    11041 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
    11101 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
    11161 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
    11221 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
    11281 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
    11341 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
    11401 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
    11461 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
    11521 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
    11581 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
    11641 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
    11701 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
    11761 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
    11821 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
    11881 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
    11941 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
    12001 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
    12061 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
    12121 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
    12181 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
    12241 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
    12301 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
    12361 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
    12421 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
    12481 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
    12541 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
    12601 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
    12661 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
    12721 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
    12781 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
    12841 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
    12901 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
    12961 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
    13021 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
    13081 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
    13141 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagc
//
