LOCUS       sequence-285303-        2036 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2724
FEATURES             Location/Qualifiers
     source          1..2036
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagatccc tgccccggag cccgagaaga cagctctttc ctctcaggat
       61 cctgctcttt ccctgaaaga gaatctcgag gatatatcgg gttggggtct tcccgaagcc
      121 aggtccaagg aatcggtgag tttcaaggat gtggctgtgg acttcaccca ggaggagtgg
      181 ggtcaactag actcccctca gagggccttg taccgggatg tgatgttgga gaactaccag
      241 aaccttcttg ccctaggacc tccactgcac aagccagatg tgatctctca tctggaacga
      301 ggcgaggagc catggagcat gcagagggaa gtccccagag ggccctgtcc agaatgggag
      361 ctgaaggcgg tgccctctca acagcagggc atttgcaaag aagaaccggc ccaggagccc
      421 atcatggagc ggcccctcgg cggggcgcag gcgtgggggc gccaggcagg tgctctgcag
      481 aggagtcagg ctgcgccctg ggcgcccgca cctgccatgg tctgggacgt ccctgtagag
      541 gaattccccc tcaggtgtcc cctcttcgcc cagcaacgcg ttcccgaggg gggacccttg
      601 ctggacacac gcaagaacgt ccaggccact gagggcagaa ccaaggcccc cgcgagactg
      661 tgtgcagggg aaaacgcctc cacgccaagt gagccagaaa agttccccca ggtgcgccgg
      721 cagcgcgggg cgggcgccgg ggagggcgag ttcgtgtgcg gcgagtgcgg gaaggcgttc
      781 cgccagagct cctccctcac gctgcaccgg cgctggcaca gccgggagaa ggcttacaag
      841 tgcgatgaat gcggcaaggc cttcacctgg agcaccaacc ttctggagca ccggcgcatc
      901 cacaccggcg agaagccctt cttctgcggc gagtgcggga aggccttcag ctgccactcg
      961 tccctcaacg tgcaccagcg catccacacg ggcgagcggc cctacaagtg cagcgcctgc
     1021 gagaaggcct tcagctgcag ctcgctgctc agcatgcacc tgcgggtgca caccggcgag
     1081 aagccctacc ggtgcggcga gtgcggcaag gccttcaacc agcgtacaca cctcacacgc
     1141 caccaccgca tccacacggg cgagaagccc taccagtgcg gctcctgcgg caaggccttc
     1201 acctgccact catccctcac cgtgcatgag aagatccaca gcggggacaa gccgttcaag
     1261 tgcagcgact gcgagaaggc cttcaacagc cgctcgcgcc tcaccctcca ccagaggacg
     1321 cacacgggcg agaagccctt caagtgcgcc gactgcggga agggcttcag ctgccacgcg
     1381 tacctgctcg tgcaccggcg catccacagc ggcgagaagc ccttcaagtg caacgagtgc
     1441 ggcaaagcct tcagctccca cgcctacctc atcgtgcacc ggcgcatcca cacaggcgag
     1501 aagcccttcg actgcagcca gtgttggaag gccttcagct gccactcgtc cctcatcgtg
     1561 caccagcgca tccacaccgg tgagaagccc tacaagtgca gcgagtgcgg cagagccttc
     1621 agccagaacc actgtctcat taaacatcag aaaatccact ccggggagaa gtcgtttaag
     1681 tgtgagaaat gtggggagat gttcaactgg agctcgcacc tcactgagca ccagaggctg
     1741 cacagcgagg ggaagccctt ggccatccag ttcaacaaac acctgctcag cacatactac
     1801 gtgcctggca gcctgctggg tgcaggggat gctggactga gggacgtgga tcccatcgac
     1861 gcgctggatg tggcaaagct cttgtgcgtg gttcccccca gagctggcag gaatttctcc
     1921 ctggggagca aacctcgaaa ctagtaatga actagtccgt aacttgaaag tatttcgatt
     1981 tcttggcttt atatatcttg tggaaaggac gaccttgggt aatatcgccg cagtga
//
