LOCUS       sequence-285305-       16240 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16240)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 16240)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2733
FEATURES             Location/Qualifiers
     source          1..16240
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    12097..12685
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(12150..12170)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(12568..12579)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             12757..12990
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    13068..13189
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13105..13124)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13159..13178
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      13229..13364
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(13384..13403)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(13385..13403)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(13413..13430)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(13413..13429)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13422..13444)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      13571..14026
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13658..13677)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13868..13889
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        14052..14156
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(14157..14225,14226..15017)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(14375..14394)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      15188..15776
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15677..15696
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     15930..15947
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    16064..16085
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(16100..16105,16106..16123,16124..16130)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    16138..16154
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     16143..16165
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     16162..16178
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     16162..16178
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     16197..16217
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        16199..16217
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgttc cgcagggcac gccttagcgt
     4141 gaagccgaat gtcaggcctg gtgtaggcgc caggggctcc acagcttcca atccccagcg
     4201 tggacgggag tctcccaggc cgccggatcc tgccacggac tctgcttcca agcccgcgga
     4261 gcccacagat gtgcccacag tcgatttcgg tggagcggag ccccaagaaa aggctcctag
     4321 gagcagtact gaaaagactg gtggtgacaa tgatgttgaa gaatccagta gatcttcctc
     4381 tactgtttca cagagaagaa agcgaatatc aagtacttct agcctggtta agtctagtgt
     4441 cagtgttcct tcagaatctc atcccttatc tacaattaat caagaggctc cacagccaac
     4501 tgccacttca acaaaagaga aacagccatg ctcagacaga taccgaatat acaaagccca
     4561 gaaactgagg gaaatgttaa aagaagaatt gagaaaagag aagaaacaat ggaaaaacaa
     4621 atatgctata aatgaaagtc agaggccacc agatcgttca aaaatgacta tgagagactt
     4681 catatattat ctaccagata ataatccaat gacttcttca ctggaacaag aaaagaaaac
     4741 tgaaaagcca tcgactccag tccagacaag agagcaagaa ggtaagagta ctcctaatgc
     4801 tgaagataat gaaatggaag aagagacaga tgatgggcca ttactggttc ctcgagtaaa
     4861 agtggcagaa gatggttcca ttattttgga tgaagaaagt ttaactgtag aagttttaag
     4921 aacaaaaggc ccttgtgttg ttgaagaaaa tgaccccata tttgagcgcg gttctacaac
     4981 tacatactcc agctttagga aaaactatta ctctaaacca tggtcaaata aagaaacaga
     5041 tatgtttttt ttagccatca gcatggtagg aactgacttt tctatgatcg gacaactttt
     5101 tcctcacaga gcaaggatag aaattaagaa taaatttaaa cgggaagaga aaacaaatgg
     5161 atggagaata gacaaagcat tccaggaaaa gcgccctttt gacttcgatt tttttgctca
     5221 tttgcttcag aaagttcttg ctgaagaaga gaaaagaaaa caaaaatctg ttaaaaatca
     5281 cagtttaaag gagaagaaat ccaccaaacc acggaaaaat gtaaaagtga aaaaagttgc
     5341 ctgtgaagga gtgaataatg atccagatga gtctatgagt tctagaattt cagacacgga
     5401 aagatctcag aaggatgctc agacagttga agaagagtct ctgaccttat caagggagga
     5461 tgcagagcag gttgcattag aagtagacct aaatcaaaag aaaagaagga ggaagaagca
     5521 agatggagct aatgaactgg gagtaaacaa tcttttagaa aatgccactg ttcaggcggg
     5581 tccttctaaa ggagaaaaac acaagaataa atgtcaggct ataaggcctg agctaaagga
     5641 aggtgaatgc agtaaggagc agatgctttc ctgcacacaa aacatagatg gcattgtggg
     5701 ttttgcctcc actgaaaaag ttgagaaaag aactgacccc atcctttcat taagtaatca
     5761 acaagatgcc acatcagtag caactgagtc ttcagaatca agcacttcag atttgccttc
     5821 attcgaagtt ggaattagag cattgtgtga ggtgaataat gctgagggta gttgtataga
     5881 agaaagaaat gttgacctaa aaaataattc actggaaatt gatcaaacag aaaatgttaa
     5941 accaatgttg agaggtcgct tccaaagacc taaacccaat ttgtcaaggg ctgggaagaa
     6001 atcagttctt tcacaaggca aaacagagtc agagagcaag aattcacatt caaaaacttc
     6061 agttgaaaag aaccacgtgg aaaaagataa aatgaataca ttggacattt tgagaatgga
     6121 gactacagag agagagaatc cagaagctga aactgtatct gttttgggtg aaaaaaattg
     6181 tctgcaggaa gggagtcaac taaaggcttt aagacctgta caagtgaggg gccgattgca
     6241 aaagccaaag ccaaatgcag gtaaagctgc tgaaagaaaa gaaattctca tatcacagga
     6301 agaaattggg gccaatgtag agaagaatga aaatgaatcc tgtgctgata gagatactcc
     6361 tcaacacatg gaagatcaat cgcgtaaaga ttttgaagag gaagatgtca tattacagcc
     6421 tgagaaaaat gattcttttc aaaatgtgca gccagatgag cccaaggttc ttaatgaatg
     6481 tctaagcgtt caagagaata ataaggcaaa taaacttaac caagtcccaa ttctaaggac
     6541 tcgatttcag aaaccaaagc caaatatagg aagaggaact ggaaggagag aaatttcctc
     6601 aaaggaagag gtactagaga agattcttgt ctctggggaa atggcggcag cattgagaga
     6661 aactgtaaga ctagacacct caccaaagga gatggtacca gcagagatta atactaaaga
     6721 aatgcagtca gatttaaaag aaactggaag aagagccatt tctcccaggg agaagattct
     6781 agatgtgatt gatgacacca tagaaatgga gacaggtctg aaagcaatgg gaagagagat
     6841 ttgtctaagg gagaagacgc cagaggtgat tgatgccact gaggaaatag acaaagattt
     6901 ggaagaagct ggaagaagag aaatatcccc acagaaaaat ggcccagagg aggttaagcc
     6961 tctaggtgaa gtggagacag atttgaaagc aactggaaat gagagttccc caagggagaa
     7021 gacaccagag gtgactgatg ccactgagga aatagacaaa aatttggaag aaactggaag
     7081 aagaaaaata tccccaaggg aaaatggccc agaggaggtc aagcctgtag atgaaatgga
     7141 gacagatttg aacgcaactg gaagagagag ttctccaagg gagaagacac cagaggtgat
     7201 tgatgctact gaggaaatag atttggaaga aactgaaaga gaagtatccc cacaggaaaa
     7261 tggactagag gaggtcaagc ctctaggtga aatggagacg gatttgaaag caactggaag
     7321 agacagtttc ccaaggggga agacaccaga ggtgattgat gccattgagg aaatagagat
     7381 agatttggaa gaaactgaaa gagaaatatc cccacaggaa aatggcctag aggaggttaa
     7441 gcctctaggt gaaatgcaaa cagatttgaa agcaaccgga agggagattt ccccaaggga
     7501 gaagacacca gaggtgattg atgccactga ggaaatagac aaagatctgg aagaaactgg
     7561 aagaagagaa atatccccag aggaaaatgg cccagaggag gtcaagcctg tagatgaaat
     7621 ggagacagac ttgaaaacaa ctggaagaga gggttcctca agggagaaga cacgagaggt
     7681 gattgatgct gctgaggtaa tagagacaga tttggaagaa actgaaagag aaatatcgcc
     7741 acaggaaaat ggcccagagg aggtcaagcc tgtaggtaaa atggagacag atttgaaaga
     7801 aattagagaa gaaatttccc aaagggaaaa ggtgctagca gagttcagtg ctataaggga
     7861 aaaggagatt gatttgaaag aaactggaaa aagagacatt cccatcatgg agaaagtatc
     7921 aggaaagatg gctgttgttg aagaaatgga ggcagatttg aaagaaactg gaaaagaaaa
     7981 ttttagagag agaggatctg aagagatctg tgttactgag gaaaaggtgg cagaattgaa
     8041 acaaactgga aaaacagaca tttctccaag ggaaaacgag ctagaggaga ccagtacctc
     8101 aagacaaact gacacacatt taatgcagag cggtagcaat gacttcagtg ctgtgccttc
     8161 actagatatt cagaacatta gcagtgaagt actgtcgatg atgcatacac ctgtagaaga
     8221 aaaaagaaat tctgaaaaag aagtatcaag tcacttcagt catttcaaga tttcttcaca
     8281 gactcatgaa tctgataaaa cagaagtcca ggggattcaa tctccagatg ttccagagca
     8341 gttttcagat attaatttaa gcaaatctct tcctcaagaa cagaagccac ttgaaattaa
     8401 accagcacct tttgtgagga gccgattcaa aagaccaaaa ccaaacttag caagagcagc
     8461 tttgaagaga gagactacag aatcagaaaa atatatatat gagaagaaat cagaaaccaa
     8521 gaaaatggag actattgtga tgcaagaaaa taatgaacaa actgatactc tcccttctca
     8581 acatgatgaa gcttccctaa tgatatcaag agaaaaagac acattaggtc acaggaatga
     8641 ggaggctgtg atattgccat gtacacagac tgaaaggaac ctttcacctt caaattcttg
     8701 tgaacctaaa gaggagtctc agtcagcacc agtccagaaa aatgactcag ttgtttctgt
     8761 ggggactaat aatgtaaaca ctttccagca agaaatgaag gaaagtgtta tccaaactgc
     8821 tcgacaagta aggggccgac ttcagagacc gagaccaaat ataagaaaga caggacagag
     8881 gcaaatagta gacaaaggtg aagccaaagg cataattaag gaaggaagaa cgatattacc
     8941 aaaagatgaa actgaaaaga aagtcttaac tgtgtcaaat tctcaaattg aaactgaaat
     9001 tgaagttcca tcgtccgcag ttccagaaca cagaatgtat gaaaatcaaa gtcaggtggt
     9061 tcttgtagaa aaccttcatg ttaacaaaac aaatgaaaca atcagacatg aaaataaacc
     9121 gtatgttcct agttcagcac aaatgacaag aaggaaattc caaaaggcta agccaaattt
     9181 gggaagagca cacagtaaga aagaggaacc agttttagaa aaagtcacaa cagatcagag
     9241 caaggaaggc aagccagaag atcatttgct gcagaaagga gcttccaaca cccagctcct
     9301 tctaaaagaa aaagctgagc ttctgacatc tctggaggtt tcagcaagaa aagattgtgt
     9361 aggttccaaa gagtctgctt tggcaaaaat agatgcggaa ttagaagaag ttggaccatc
     9421 aagaagggtt ggagaggaaa ctgtaggaga taattcacca tcttcagttg ttgaagagca
     9481 atatctcaat aaactaacaa gctgtccaca accgttaaac gaaacaagtt actctaaaat
     9541 tgccctggat gggaaaacaa ctatctcttc tacatctgag tatgagagaa atcgtggtga
     9601 aaggagaagt cataaaaagt tcaaaccaaa tgtcaccaga ggtcgtggat caaaacgagt
     9661 tcggggtaag acctctaaga aggaacctag agcttccaag gccatgctgg tgactcttcg
     9721 ggcttcccag gaagaagatg atgatgctga cgattttgag tctgactatg aggaagaaag
     9781 ctatcatctt gctcccgaag aagtaaacaa agctccagta tttgtacctg ttggtctcag
     9841 atctcctgaa cctgtttctg ctcagattga ggaaacaatg gaagagcttg aaataactgt
     9901 gaatgtccca gatgtaggat gcatagctgt tgttgaacat gagctaccaa acacagatgt
     9961 gactactgaa gaaatgaaac aagaagaaaa tttgagtgta ccgtttgaaa tgaccacaag
    10021 tgaacatatc caagatgaac caggtaccaa tgatggaagc accgaagctg caataacttt
    10081 acttacaatg ggagatctag tattgcagtc agagatcagt agtgaacagg gtgatgtagg
    10141 agtatgtata attcctcatg ttcattcaaa ggataaaagc catattcctt ctagcctaga
    10201 taatgtaaat cacaaaattg ttcatgaatg tcaggaactt tcttcacctg tcattactac
    10261 atctcctgca tcatttgaag aaaacaagat tgtattggag gaacaaagtt ccagagaaga
    10321 aataagttta atggagaaag taaaggagaa tgctacacca accaggaata caatttctaa
    10381 agtgaccagt aatttgagaa taagaagtag gcttgctaag cctaaaccaa atcttgagaa
    10441 gactttaggg accaacaggc ttgatgatta tcaggaagtt tccagtttgt gtgtaaccaa
    10501 aggggcagaa atggaaactc aaagagaaac agagaaaaat gcttccaaag caacagaatt
    10561 ggaaaataaa aacctcggac cagttacaac agcagagaat aaggatcaga gcaaattggc
    10621 atgtgtacat ggtatcaaag ggaccagtat ttcttcagaa gtaaacctaa ctgaaagaaa
    10681 cgaaaatcaa gaagagagct ctcaggaggt tcacatgttg tcagttgctc cagttgcttc
    10741 ctctgagaca gggccctgca cacttggttt ggataggggt cttggtgaaa attctgttga
    10801 agagccccag ataaaggact ctaaaggaga cagtgtgctt acacttcctg tgccagagta
    10861 tacaccaaca agtattccag aagtccaaca agagaatata atcaatcctc aagacctaac
    10921 agtgaatcta gttgctaatg tacctcaaga tggagaagat gaacaagcct ttattttaac
    10981 tctggtggaa atcccagcca atgcagtaga agaatttact gatgccactg cacagttcat
    11041 gccaaaccct ttactgccag ctcccatatt ggtcaaatca gtgaataccg aagaaagggg
    11101 tgacatgagt atttgtttac cagcaacttc agttggtcaa gatgccatgg gtttatctat
    11161 ttctggaaga gataattcta aaaagccgcc tgataatttg gatcttgtat ctaggaagag
    11221 atttcaatgc aggcttgata aaaatgacca cattcctcct gccaaaaaac gttcactcac
    11281 tttaagagat gactgtcaag aatataccac tgaggtgcac tcaaaggaat taacaaatgt
    11341 ttttgaggaa acaggggagt ctcacaaggg acaagatatt tttcttacct caggaagcac
    11401 actgacaact ccagaacctc aaagacagca agttgaagca gcttttcaga gtagaggatc
    11461 tagatctcct gatgcatgca tggacaagaa tgtgcctcag ttacctcagg atgaaatgat
    11521 tgtgtctgat aaggaagaaa gaactgatgc tgctcctaag tctcagcaaa tggatagcag
    11581 aacatcgtct tctaaagcct cactatccag acctggcaga agacccctgg gatttttatc
    11641 tttaatatgc tcaaagaata gtttggagtc tgatgaacct atgcaagtcc atagtaagaa
    11701 acgcctaaaa cctcttatac ctggattaag aaagaaattg aaaagatcta atccattcaa
    11761 tgaaagccag gaaaaaaatc gagagtcctc tgatctgctt ccatctccaa gtgttattac
    11821 tactcaatct gagaatatta gcagctcagc aactcaggtt tcttgtgatc agcccttact
    11881 gaaagaagga tataaaagtg cccaaaagcg ggcccctcaa ggggaggcaa ccacagtctc
    11941 tgaatatttc ttcaatgata tcttcattga agtggatgaa acagaatagt aatgaactag
    12001 tccgtaactt gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgagat
    12061 accgaggcca aacagcaata tacgcgttaa gtcgacaatc aacctctgga ttacaaaatt
    12121 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
    12181 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
    12241 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
    12301 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
    12361 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
    12421 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
    12481 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
    12541 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
    12601 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
    12661 atctcccttt gggccgcctc cccgcgtcga ctttaagacc aatgacttac aaggcagctg
    12721 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
    12781 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
    12841 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
    12901 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
    12961 ccttttagtc agtgtggaaa atctctagca gtacgtatag tagttcatgt catcttatta
    13021 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
    13081 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
    13141 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
    13201 tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
    13261 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
    13321 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat
    13381 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac
    13441 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
    13501 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
    13561 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
    13621 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
    13681 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
    13741 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
    13801 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
    13861 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
    13921 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
    13981 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc
    14041 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
    14101 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
    14161 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
    14221 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
    14281 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
    14341 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
    14401 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
    14461 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
    14521 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
    14581 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
    14641 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
    14701 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
    14761 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
    14821 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
    14881 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
    14941 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
    15001 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
    15061 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
    15121 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
    15181 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
    15241 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
    15301 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
    15361 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
    15421 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
    15481 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
    15541 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
    15601 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
    15661 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
    15721 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
    15781 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
    15841 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
    15901 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
    15961 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
    16021 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
    16081 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
    16141 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa
    16201 ttaaccctca ctaaagggaa caaaagctgg agctgcaagc
//
