LOCUS       sequence-285306-        7976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7976)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 7976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2733
FEATURES             Location/Qualifiers
     source          1..7976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 accgccacca tgttccgcag ggcacgcctt agcgtgaagc cgaatgtcag gcctggtgta
       61 ggcgccaggg gctccacagc ttccaatccc cagcgtggac gggagtctcc caggccgccg
      121 gatcctgcca cggactctgc ttccaagccc gcggagccca cagatgtgcc cacagtcgat
      181 ttcggtggag cggagcccca agaaaaggct cctaggagca gtactgaaaa gactggtggt
      241 gacaatgatg ttgaagaatc cagtagatct tcctctactg tttcacagag aagaaagcga
      301 atatcaagta cttctagcct ggttaagtct agtgtcagtg ttccttcaga atctcatccc
      361 ttatctacaa ttaatcaaga ggctccacag ccaactgcca cttcaacaaa agagaaacag
      421 ccatgctcag acagataccg aatatacaaa gcccagaaac tgagggaaat gttaaaagaa
      481 gaattgagaa aagagaagaa acaatggaaa aacaaatatg ctataaatga aagtcagagg
      541 ccaccagatc gttcaaaaat gactatgaga gacttcatat attatctacc agataataat
      601 ccaatgactt cttcactgga acaagaaaag aaaactgaaa agccatcgac tccagtccag
      661 acaagagagc aagaaggtaa gagtactcct aatgctgaag ataatgaaat ggaagaagag
      721 acagatgatg ggccattact ggttcctcga gtaaaagtgg cagaagatgg ttccattatt
      781 ttggatgaag aaagtttaac tgtagaagtt ttaagaacaa aaggcccttg tgttgttgaa
      841 gaaaatgacc ccatatttga gcgcggttct acaactacat actccagctt taggaaaaac
      901 tattactcta aaccatggtc aaataaagaa acagatatgt tttttttagc catcagcatg
      961 gtaggaactg acttttctat gatcggacaa ctttttcctc acagagcaag gatagaaatt
     1021 aagaataaat ttaaacggga agagaaaaca aatggatgga gaatagacaa agcattccag
     1081 gaaaagcgcc cttttgactt cgattttttt gctcatttgc ttcagaaagt tcttgctgaa
     1141 gaagagaaaa gaaaacaaaa atctgttaaa aatcacagtt taaaggagaa gaaatccacc
     1201 aaaccacgga aaaatgtaaa agtgaaaaaa gttgcctgtg aaggagtgaa taatgatcca
     1261 gatgagtcta tgagttctag aatttcagac acggaaagat ctcagaagga tgctcagaca
     1321 gttgaagaag agtctctgac cttatcaagg gaggatgcag agcaggttgc attagaagta
     1381 gacctaaatc aaaagaaaag aaggaggaag aagcaagatg gagctaatga actgggagta
     1441 aacaatcttt tagaaaatgc cactgttcag gcgggtcctt ctaaaggaga aaaacacaag
     1501 aataaatgtc aggctataag gcctgagcta aaggaaggtg aatgcagtaa ggagcagatg
     1561 ctttcctgca cacaaaacat agatggcatt gtgggttttg cctccactga aaaagttgag
     1621 aaaagaactg accccatcct ttcattaagt aatcaacaag atgccacatc agtagcaact
     1681 gagtcttcag aatcaagcac ttcagatttg ccttcattcg aagttggaat tagagcattg
     1741 tgtgaggtga ataatgctga gggtagttgt atagaagaaa gaaatgttga cctaaaaaat
     1801 aattcactgg aaattgatca aacagaaaat gttaaaccaa tgttgagagg tcgcttccaa
     1861 agacctaaac ccaatttgtc aagggctggg aagaaatcag ttctttcaca aggcaaaaca
     1921 gagtcagaga gcaagaattc acattcaaaa acttcagttg aaaagaacca cgtggaaaaa
     1981 gataaaatga atacattgga cattttgaga atggagacta cagagagaga gaatccagaa
     2041 gctgaaactg tatctgtttt gggtgaaaaa aattgtctgc aggaagggag tcaactaaag
     2101 gctttaagac ctgtacaagt gaggggccga ttgcaaaagc caaagccaaa tgcaggtaaa
     2161 gctgctgaaa gaaaagaaat tctcatatca caggaagaaa ttggggccaa tgtagagaag
     2221 aatgaaaatg aatcctgtgc tgatagagat actcctcaac acatggaaga tcaatcgcgt
     2281 aaagattttg aagaggaaga tgtcatatta cagcctgaga aaaatgattc ttttcaaaat
     2341 gtgcagccag atgagcccaa ggttcttaat gaatgtctaa gcgttcaaga gaataataag
     2401 gcaaataaac ttaaccaagt cccaattcta aggactcgat ttcagaaacc aaagccaaat
     2461 ataggaagag gaactggaag gagagaaatt tcctcaaagg aagaggtact agagaagatt
     2521 cttgtctctg gggaaatggc ggcagcattg agagaaactg taagactaga cacctcacca
     2581 aaggagatgg taccagcaga gattaatact aaagaaatgc agtcagattt aaaagaaact
     2641 ggaagaagag ccatttctcc cagggagaag attctagatg tgattgatga caccatagaa
     2701 atggagacag gtctgaaagc aatgggaaga gagatttgtc taagggagaa gacgccagag
     2761 gtgattgatg ccactgagga aatagacaaa gatttggaag aagctggaag aagagaaata
     2821 tccccacaga aaaatggccc agaggaggtt aagcctctag gtgaagtgga gacagatttg
     2881 aaagcaactg gaaatgagag ttccccaagg gagaagacac cagaggtgac tgatgccact
     2941 gaggaaatag acaaaaattt ggaagaaact ggaagaagaa aaatatcccc aagggaaaat
     3001 ggcccagagg aggtcaagcc tgtagatgaa atggagacag atttgaacgc aactggaaga
     3061 gagagttctc caagggagaa gacaccagag gtgattgatg ctactgagga aatagatttg
     3121 gaagaaactg aaagagaagt atccccacag gaaaatggac tagaggaggt caagcctcta
     3181 ggtgaaatgg agacggattt gaaagcaact ggaagagaca gtttcccaag ggggaagaca
     3241 ccagaggtga ttgatgccat tgaggaaata gagatagatt tggaagaaac tgaaagagaa
     3301 atatccccac aggaaaatgg cctagaggag gttaagcctc taggtgaaat gcaaacagat
     3361 ttgaaagcaa ccggaaggga gatttcccca agggagaaga caccagaggt gattgatgcc
     3421 actgaggaaa tagacaaaga tctggaagaa actggaagaa gagaaatatc cccagaggaa
     3481 aatggcccag aggaggtcaa gcctgtagat gaaatggaga cagacttgaa aacaactgga
     3541 agagagggtt cctcaaggga gaagacacga gaggtgattg atgctgctga ggtaatagag
     3601 acagatttgg aagaaactga aagagaaata tcgccacagg aaaatggccc agaggaggtc
     3661 aagcctgtag gtaaaatgga gacagatttg aaagaaatta gagaagaaat ttcccaaagg
     3721 gaaaaggtgc tagcagagtt cagtgctata agggaaaagg agattgattt gaaagaaact
     3781 ggaaaaagag acattcccat catggagaaa gtatcaggaa agatggctgt tgttgaagaa
     3841 atggaggcag atttgaaaga aactggaaaa gaaaatttta gagagagagg atctgaagag
     3901 atctgtgtta ctgaggaaaa ggtggcagaa ttgaaacaaa ctggaaaaac agacatttct
     3961 ccaagggaaa acgagctaga ggagaccagt acctcaagac aaactgacac acatttaatg
     4021 cagagcggta gcaatgactt cagtgctgtg ccttcactag atattcagaa cattagcagt
     4081 gaagtactgt cgatgatgca tacacctgta gaagaaaaaa gaaattctga aaaagaagta
     4141 tcaagtcact tcagtcattt caagatttct tcacagactc atgaatctga taaaacagaa
     4201 gtccagggga ttcaatctcc agatgttcca gagcagtttt cagatattaa tttaagcaaa
     4261 tctcttcctc aagaacagaa gccacttgaa attaaaccag caccttttgt gaggagccga
     4321 ttcaaaagac caaaaccaaa cttagcaaga gcagctttga agagagagac tacagaatca
     4381 gaaaaatata tatatgagaa gaaatcagaa accaagaaaa tggagactat tgtgatgcaa
     4441 gaaaataatg aacaaactga tactctccct tctcaacatg atgaagcttc cctaatgata
     4501 tcaagagaaa aagacacatt aggtcacagg aatgaggagg ctgtgatatt gccatgtaca
     4561 cagactgaaa ggaacctttc accttcaaat tcttgtgaac ctaaagagga gtctcagtca
     4621 gcaccagtcc agaaaaatga ctcagttgtt tctgtgggga ctaataatgt aaacactttc
     4681 cagcaagaaa tgaaggaaag tgttatccaa actgctcgac aagtaagggg ccgacttcag
     4741 agaccgagac caaatataag aaagacagga cagaggcaaa tagtagacaa aggtgaagcc
     4801 aaaggcataa ttaaggaagg aagaacgata ttaccaaaag atgaaactga aaagaaagtc
     4861 ttaactgtgt caaattctca aattgaaact gaaattgaag ttccatcgtc cgcagttcca
     4921 gaacacagaa tgtatgaaaa tcaaagtcag gtggttcttg tagaaaacct tcatgttaac
     4981 aaaacaaatg aaacaatcag acatgaaaat aaaccgtatg ttcctagttc agcacaaatg
     5041 acaagaagga aattccaaaa ggctaagcca aatttgggaa gagcacacag taagaaagag
     5101 gaaccagttt tagaaaaagt cacaacagat cagagcaagg aaggcaagcc agaagatcat
     5161 ttgctgcaga aaggagcttc caacacccag ctccttctaa aagaaaaagc tgagcttctg
     5221 acatctctgg aggtttcagc aagaaaagat tgtgtaggtt ccaaagagtc tgctttggca
     5281 aaaatagatg cggaattaga agaagttgga ccatcaagaa gggttggaga ggaaactgta
     5341 ggagataatt caccatcttc agttgttgaa gagcaatatc tcaataaact aacaagctgt
     5401 ccacaaccgt taaacgaaac aagttactct aaaattgccc tggatgggaa aacaactatc
     5461 tcttctacat ctgagtatga gagaaatcgt ggtgaaagga gaagtcataa aaagttcaaa
     5521 ccaaatgtca ccagaggtcg tggatcaaaa cgagttcggg gtaagacctc taagaaggaa
     5581 cctagagctt ccaaggccat gctggtgact cttcgggctt cccaggaaga agatgatgat
     5641 gctgacgatt ttgagtctga ctatgaggaa gaaagctatc atcttgctcc cgaagaagta
     5701 aacaaagctc cagtatttgt acctgttggt ctcagatctc ctgaacctgt ttctgctcag
     5761 attgaggaaa caatggaaga gcttgaaata actgtgaatg tcccagatgt aggatgcata
     5821 gctgttgttg aacatgagct accaaacaca gatgtgacta ctgaagaaat gaaacaagaa
     5881 gaaaatttga gtgtaccgtt tgaaatgacc acaagtgaac atatccaaga tgaaccaggt
     5941 accaatgatg gaagcaccga agctgcaata actttactta caatgggaga tctagtattg
     6001 cagtcagaga tcagtagtga acagggtgat gtaggagtat gtataattcc tcatgttcat
     6061 tcaaaggata aaagccatat tccttctagc ctagataatg taaatcacaa aattgttcat
     6121 gaatgtcagg aactttcttc acctgtcatt actacatctc ctgcatcatt tgaagaaaac
     6181 aagattgtat tggaggaaca aagttccaga gaagaaataa gtttaatgga gaaagtaaag
     6241 gagaatgcta caccaaccag gaatacaatt tctaaagtga ccagtaattt gagaataaga
     6301 agtaggcttg ctaagcctaa accaaatctt gagaagactt tagggaccaa caggcttgat
     6361 gattatcagg aagtttccag tttgtgtgta accaaagggg cagaaatgga aactcaaaga
     6421 gaaacagaga aaaatgcttc caaagcaaca gaattggaaa ataaaaacct cggaccagtt
     6481 acaacagcag agaataagga tcagagcaaa ttggcatgtg tacatggtat caaagggacc
     6541 agtatttctt cagaagtaaa cctaactgaa agaaacgaaa atcaagaaga gagctctcag
     6601 gaggttcaca tgttgtcagt tgctccagtt gcttcctctg agacagggcc ctgcacactt
     6661 ggtttggata ggggtcttgg tgaaaattct gttgaagagc cccagataaa ggactctaaa
     6721 ggagacagtg tgcttacact tcctgtgcca gagtatacac caacaagtat tccagaagtc
     6781 caacaagaga atataatcaa tcctcaagac ctaacagtga atctagttgc taatgtacct
     6841 caagatggag aagatgaaca agcctttatt ttaactctgg tggaaatccc agccaatgca
     6901 gtagaagaat ttactgatgc cactgcacag ttcatgccaa accctttact gccagctccc
     6961 atattggtca aatcagtgaa taccgaagaa aggggtgaca tgagtatttg tttaccagca
     7021 acttcagttg gtcaagatgc catgggttta tctatttctg gaagagataa ttctaaaaag
     7081 ccgcctgata atttggatct tgtatctagg aagagatttc aatgcaggct tgataaaaat
     7141 gaccacattc ctcctgccaa aaaacgttca ctcactttaa gagatgactg tcaagaatat
     7201 accactgagg tgcactcaaa ggaattaaca aatgtttttg aggaaacagg ggagtctcac
     7261 aagggacaag atatttttct tacctcagga agcacactga caactccaga acctcaaaga
     7321 cagcaagttg aagcagcttt tcagagtaga ggatctagat ctcctgatgc atgcatggac
     7381 aagaatgtgc ctcagttacc tcaggatgaa atgattgtgt ctgataagga agaaagaact
     7441 gatgctgctc ctaagtctca gcaaatggat agcagaacat cgtcttctaa agcctcacta
     7501 tccagacctg gcagaagacc cctgggattt ttatctttaa tatgctcaaa gaatagtttg
     7561 gagtctgatg aacctatgca agtccatagt aagaaacgcc taaaacctct tatacctgga
     7621 ttaagaaaga aattgaaaag atctaatcca ttcaatgaaa gccaggaaaa aaatcgagag
     7681 tcctctgatc tgcttccatc tccaagtgtt attactactc aatctgagaa tattagcagc
     7741 tcagcaactc aggtttcttg tgatcagccc ttactgaaag aaggatataa aagtgcccaa
     7801 aagcgggccc ctcaagggga ggcaaccaca gtctctgaat atttcttcaa tgatatcttc
     7861 attgaagtgg atgaaacaga atagtaatga actagtccgt aacttgaaag tatttcgatt
     7921 tcttggcttt atatatcttg tggaaaggac gagataccga ggccaaacag caatat
//
