LOCUS       sequence-28532-e         714 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Nam HS, Benezra R
  TITLE     High levels of Id1 expression define B1 type adult neural stem 
            cells.
  JOURNAL   Cell Stem Cell. 2009 Nov 6. 5(5):515-26.
  PUBMED    19896442
REFERENCE   2  (bases 1 to 714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2009 Nov 6. 5(5):515-26."
COMMENT     Sequence Label: pKO2.2
FEATURES             Location/Qualifiers
     source          1..714
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(187..291)
                     /gene="bla"
                     /label=AmpR promoter
     regulatory      335..344
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(414..430)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    450..584
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(474..493)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     528..547
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     intron          645..710
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
ORIGIN
        1 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
       61 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
      121 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
      181 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcgtgag
      241 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
      301 ccgaaaagtg ccacctgacg tcaaacagct atgaccatgg cggccgctaa ctataacggt
      361 cctaaggtag cgaagatctc ctagaggtac cctagagaat tcctagagtt aacactggcc
      421 gtcgttttac cggtccgctc tagaactacg atccagacat gataagatac attgatgagt
      481 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
      541 ctattgcttt atttgtaacc attataagct gcaataaaca agttagatcc tagtggatct
      601 gcattccacc actgctccca ttcatcagtt ccataggttg gaatctaaaa tacacaaaca
      661 attagaatca gtagtttaac acattataca cttaaaaatt ttatatttac ctta
//
