LOCUS       sequence-285321-        2171 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2047
FEATURES             Location/Qualifiers
     source          1..2171
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaggatac tggcatccag cgaggcatct gggatggaga tgccaaggct
       61 gtccaacaat gtctgacaga tatttttacc agcgtttaca ccacctgcga catccctgag
      121 aatgctatat ttggtccctg tgtcctgagc catacttccc tatatgacag catagctttc
      181 atagctctca agtctactga caagagaaca gtaccgtata tctttcgggt agacacctca
      241 gcagcaaatg gttcctcaga aggtctcatg tggctgcggt tggtccaatc ggccagagat
      301 aaggaagagc agaaccttga agcctacata aaaaacggac agctgttcta ccgctctctc
      361 cgcaggattg ccaaagacga ggagttacta gtttggtacg ggaaagaact gactgagtta
      421 ctcttgctct gcccctctag atcccacaac aaaatgaatg ggtcgtcccc ttacacatgc
      481 ctggaatgca gccaacgttt ccagtttgag ttcccctatg tggcgcatct gcgtttccgc
      541 tgccccaaga gacttcacag cgctgatata agtccccaag acgaacaagg cggcggcgtg
      601 ggcaccaagg accacggggg cggcggcggc ggtggcaaag accagcagca gcagcagcag
      661 gaagccccgc tgggcccggg cccgaaattc tgcaaagccg gcccgctgca tcactatccg
      721 agtccgagcc cggaaagcag caatccaagc gcggcggcgg gtggtagcag cgccaaacca
      781 agcaccgatt tccataatct ggcgcgcgaa ctggaaaata gtcgcggtgg cagcagctgc
      841 agcccggccc agagtctgag tagtggcagc ggtagcggtg gcggtggtgg tcatcaagaa
      901 gcggaactga gcccagatgg cattgccacc ggtggcggca aaggcaaacg caaattcccg
      961 gaggaagccg cggaaggtgg tggtggtgcc ggtctggttg gcggtcgtgg ccgctttgtt
     1021 gaacgcccac tgccggccag caaagaagat ctggtgtgca ccccgcagca gtatcgtgcg
     1081 agcggcagct acttcggtct ggaagaaaat ggtcgtctgt tcgccccgcc aagcccagaa
     1141 accggcgaag ccaaacgcag tgccttcgtg gaagttaaaa aagcggcccg cgccgcgagt
     1201 ctgcaagaag aaggtaccgc cgatggtgcg ggtgttgcca gcgaagatca agatgccggc
     1261 ggtggtggcg gtagtagtac gccagcggcc gccagtccag ttggcgcgga aaaactgctg
     1321 gccccacgtc cgggcggccc actgccaagt cgtctggaag gtggtagccc agcccgcggt
     1381 agcgccttta cttcggtgcc gcagctgggc agcgcgggca gcacctctgg cggtggtggt
     1441 accggtgctg gtgctgctgg tggtgctggt ggtggtcaag gtgctgcttc tgatgaacgt
     1501 aagtctgctt tttctcaacc ggctcgttcc ttttctcaac tctctccact cgttctcggt
     1561 caaaaactcg gtgctctgga accgtgtcat ccggctgatg gtgttggtcc gactcgtctg
     1621 tacccggctg ctgctgatcc gctggctgtt aaactgcaag gtgctgctga tctcaatggt
     1681 ggctgtggtt ctctcccatc tggtggtggt ggtctcccga aacaatctcc gtttctctat
     1741 gctactgctt tttggccgaa atcttctgca gctgcagctg ctgctgcagc tgctgctgct
     1801 gctggtccgc tgcaactcca actcccgtct gctctgaccc tcctcccacc gtcttttact
     1861 tctctctgcc tcccggctca aaattggtgt gctaaatgta acgcttcttt tcgtatgact
     1921 tctgatctcg tttatcacat gcgttctcat cataagaaag aatacgctat ggaaccgctg
     1981 gttaaacgtc gtcgtgaaga aaagctgaaa tgtccgattt gtaacgaatc ttttcgtgaa
     2041 cgtcatcatc tgtctcgtca tatgacttct cacaactagt aatgaactag tccgtaactt
     2101 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgaacc tcccacagga
     2161 ccgttagaaa g
//
