LOCUS       sequence-285327-        5600 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5600)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 5600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2425
FEATURES             Location/Qualifiers
     source          1..5600
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      5009..5018
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaggacac acccccctca ctcagctgct ccgactgtca gcgccacttt
       61 cccagcctcc cagagctctc tcggcaccga gaactgctcc atccatctcc caaccaggac
      121 agtgaggagg ctgacagcat ccctcggccc taccgttgtc agcagtgtgg gcggggctac
      181 cgtcaccccg ggagcctggt taaccatcgt cggacccacg agactggcct tttcccctgt
      241 accacctgtg gcaaggactt ctccaatccc atggctctca agagccatat gaggacacat
      301 gctcctgagg gccgccgcag gcacaggccc ccacgcccca aggaagccac tccacacctc
      361 cagggtgaga cggtgtccac tgactcctgg ggccaaaggc ttggctctag tgaaggctgg
      421 gaaaaccaga caaaacatac agaagagaca cctgactgtg aatctgtacc tgaccccagg
      481 gcagcttcgg gtacgtggga agatctgccc accagacaaa gagaaggctt ggcaagccac
      541 ccaggtcctg aggatggtgc agacggctgg ggaccctcca ctaactctgc cagagcccct
      601 cctctcccca tcccagccag cagccttctt agcaacttgg aacagtatct ggctgaatca
      661 gtagtgaact tcacaggggg ccaggagccc acccagtccc ctcctgctga ggaggagcgg
      721 cggtacaaat gtagtcagtg tggcaagacc tacaagcacg ccgggagcct caccaaccac
      781 cgccagagcc acacgctggg catctacccc tgtgccatct gtttcaagga gttctctaac
      841 ctcatggctc tgaagaacca ctctcgactg catgcccagt atcggcctta ccactgtccc
      901 cactgccccc gtgtcttccg gctcccccgg gagctgctgg aacaccagca gtcccatgag
      961 ggtgaaaggc aggagccacg ctgggaggag aaagggatgc ccaccaccaa tgggcacaca
     1021 gatgagagca gccaggacca gctccccagt gcacagatgc tgaatggctc tgcggagctc
     1081 agcacctctg gggagctgga ggacagtggc ctggaggaat accggccttt ccgctgtggg
     1141 gactgtggcc gtacttaccg ccatgctggg agcctcatca accatcgaaa gagccaccag
     1201 acaggtgtct acccctgctc actctgttct aagcagctgt tcaatgcggc tgccctcaaa
     1261 aaccatgtgc gggctcatca caggcccagg caaggagttg gggaaaatgg gcagccatca
     1321 gtcccaccag ctcccctgct gctggctgag accacccaca aagaggaaga ggaccccacc
     1381 accaccctgg accatcggcc ctataagtgc agtgagtgtg gtcgtgctta ccgccaccgg
     1441 gggagcctgg tgaaccatcg ccacagccat cggactggag agtaccagtg ctcactctgt
     1501 ccccgcaagt accccaatct catggccctg cgcaaccacg tgcgggtaca ttgcaaggct
     1561 gctcgccgaa gtgcagacat cggggctgag ggtgccccca gccacctcaa ggtagaactc
     1621 ccgcctgacc cagtggaggc agaggcagcc ccgcacacag atcaggacca tgtgtgcaaa
     1681 catgaagaag aggccacgga catcacccca gcagcagaca agacagcagc acatatctgt
     1741 agcatctgtg ggctgctctt tgaagacgct gagagccttg aacgtcatgg cctgactcat
     1801 ggggcagggg aaaaggaaaa tagcagaaca gagaccacaa tgtcacctcc tagggccttt
     1861 gcctgccgag actgtggaaa gagctatcgc cactcaggca gccttatcaa ccacaggcag
     1921 acccaccaga caggagactt cagttgtggg gcctgtgcca agcacttcca caccatggct
     1981 gccatgaaga accacttgcg ccggcacagt cggcggcgga gcaggcggca tcggaagcgg
     2041 gctggcggtg ccagcggtgg gagagaagcc aaactcctgg cagcggagag ctggacccgg
     2101 gagctagaag acaatgaagg cctggagtct ccccaagacc cttcagggga aagtcctcat
     2161 ggggctgaag gcaacctgga aagtgatggg gactgtttgc aggctgaatc tgaaggggac
     2221 aaatgtgggc ttgagaggga tgagacccat ttccagggtg ataaagagag cggaggcact
     2281 ggggaaggac tggaaaggaa ggatgccagt ttacttgaca acttggacat cccaggtgag
     2341 gaaggtggtg gcactcactt ctgcgatagc ctcactgggg tggatgaaga ccagaagcca
     2401 gccactggcc aacccaactc ctcttcccac tctgccaatg ctgtcactgg ctggcaggct
     2461 ggggccgctc acacatgctc tgactgtggg cattctttcc cccatgccac tggcctgctg
     2521 agccacaggc cctgccaccc accaggcatc tatcagtgct ccctctgccc gaaggagttt
     2581 gactctctgc ctgccctccg cagccacttc cagaaccata ggcctgggga ggcgacctca
     2641 gcacagcctt tcctctgctg cctctgtggc atgatcttcc ctgggcgggc tggctacagg
     2701 cttcaccggc gccaggccca cagctcctct ggcatgactg agggctcaga ggaggagggg
     2761 gaagaggaag gagtggcaga ggcagcccct gcacgcagtc caccactgca gctctcggaa
     2821 gcagagctgc tgaatcagct gcagcgggag gtggaagcgc tggacagtgc agggtatggg
     2881 cacatctgtg gctgctgtgg tcagacctac gatgacctgg ggagcctgga gcgtcaccac
     2941 caaagtcaga gttctgggac tactgcagac aaggctccca gccccttggg agtggcaggt
     3001 gatgccatgg agatggtcgt ggacagtgtc ttggaggaca tagtgaattc tgtctctgga
     3061 gagggtggag atgccaagtc tcaagaggga gcaggcaccc ccttgggaga cagcctctgc
     3121 atccagggtg gggaaagttt gttggaggct cagccccgcc ccttccgctg caaccagtgt
     3181 ggcaagacct atcgccatgg gggcagcctg gtgaaccacc gcaagatcca ccagactgga
     3241 gactttctct gccctgtctg ctcccgctgc taccccaacc tggctgccta ccgtaatcat
     3301 ctgcggaacc accctcgctg caaaggctct gagccccagg ttgggcccat cccagaggca
     3361 gcaggtagca gtgagctgca ggttgggccc atcccagaag gaggcagcaa caagccccag
     3421 cacatggcag aggaggggcc ggggcaagca gaagtcgaga agctccagga agaacttaaa
     3481 gtggagcccc tggaggaagt ggccagggtg aaagaagagg tgtgggagga gaccactgtg
     3541 aagggggagg agatagagcc caggctggag actgccgaga agggctgcca gactgaagcc
     3601 agctctgagc ggcccttcag ctgcgaggtg tgtggccgat cctacaagca cgccggcagc
     3661 ctcatcaacc accggcagag ccaccagacc ggccactttg gctgtcaggc ctgctccaag
     3721 ggcttctcaa acctcatgtc cctcaagaac caccggcgca tccatgcaga tccccgacgt
     3781 ttccgctgca gcgagtgtgg gaaggccttc cgcctgcgga aacagctggc cagccaccag
     3841 cgggtccaca tggaacggcg tgggggtggg ggcacccgaa aggcgactcg ggaagatcgg
     3901 cccttccgct gtgggcagtg cgggcggacc tatcgccacg ccggcagcct cctgaaccac
     3961 cggcgcagcc acgagacggg ccagtacagc tgccccacct gccccaagac ctactccaac
     4021 cgcatggccc tgaaggacca ccagaggctg cactcagaga atcggcggcg acgggctgga
     4081 cggtccaggc gcacagctgt gcgttgcgcc ctctgtggcc gcagcttccc tggccgggga
     4141 tctttggagc ggcacctgcg ggagcatgag gagacagaaa gggagccagc caatggccag
     4201 ggaggcctgg atggcacagc ggccagtgag gcgaacctga ctggcagcca gggactagag
     4261 acccaattgg gtggtgctga gccagtaccc cacttggagg atggagtccc aaggccaggg
     4321 gagcgcagtc agagccccat cagggcagca agctcagaag ccccagagcc actgtcctgg
     4381 ggtgcaggga aggcaggtgg gtggccggta ggtgggggac tggggaatca tagtggaggc
     4441 tgggttcctc agttcctaac taggtcagag gagccagagg acagtgtcca caggagtcct
     4501 tgccacgctg gtgactgcca gctcaatgga cctactctga gtcacatgga tagctgggac
     4561 aacagagaca acagctctca gctgcagcca gggagccact cctcttgcag ccagtgtggc
     4621 aagacttact gccagtcagg cagcctcttg aaccacaaca ccaacaagac agaccgacac
     4681 tattgcctgc tctgctccaa ggagttctta aatcctgtgg ccacaaagag ccacagccac
     4741 aaccacatag acgcccagac ctttgcctgt cctgactgtg gcaaagcctt tgagtcccac
     4801 caggaactgg ccagccacct gcaggctcat gcccggggcc acagccaggt gccagcccag
     4861 atggaggagg ccagagatcc caaagccggg actggggagg accaggtggt tctccctggt
     4921 caagggaaag cccaggaggc cccatcagaa acccccagag gcccaggaga gagtgtggag
     4981 agagccaggg gaggacaagc ggtgacgtcc atggcggctg aggacaagga gcggcccttc
     5041 cgctgcaccc agtgcgggcg ctcctaccgc catgctggca gcctgctgaa ccaccagaag
     5101 gcccacacca cagggttgta cccgtgctcc ctctgtccca aacttctccc taacctgctg
     5161 tctcttaaga accacagcag gacccacacg gaccccaagc gccactgctg cagcatctgt
     5221 ggcaaggcct ttcggacagc tgcccggctg gagggccacg ggcgggtcca tgcaccccgg
     5281 gaggggcctt tcacctgccc ccattgtccc cgccacttcc gccgccgaat cagcttcgtg
     5341 cagcaccagc agcagcacca ggaggagtgg acggtggccg gctccggagc cccagtggca
     5401 ccagtgacgg gcagagggga cttgccattg ccccctccac ccacccccac gaccccactc
     5461 ctggatcctt caccccagtg gcctgcagac ctcagcttct ccctctagta atgaactagt
     5521 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgatgca
     5581 cgcaacttgg ctataatgct
//
