LOCUS       sequence-285330-        2447 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2504
FEATURES             Location/Qualifiers
     source          1..2447
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaaccggc gccagcgcgt agtccacgtc cacagcaaga tccagcccgc
       61 ccgcaagaac caacgatgcc accaccagaa accccaagcg aaggccgcca gccaagcccg
      121 agtccaagtc cgacggaacg tgccccggcg agtgaagagg aattccagtt tctgcgctgc
      181 cagcagtgtc aagccgaagc caaatgcccg aaactgctgc catgtctgca tacgctgtgc
      241 agcggttgtc tggaagccag tggtatgcag tgcccaattt gccaagcccc gtggccactg
      301 ggcgccgata cgccagccct cgacaacgtg tttttcgaaa gtctgcagcg tcgtctgagc
      361 gtttaccgcc agattgtgga cgcccaagcc gtttgcaccc gttgcaagga aagtgcggac
      421 ttttggtgct tcgagtgcga acagctgctg tgcgccaaat gctttgaagc gcaccagtgg
      481 tttctgaaac acgaagcccg tccactggcg gaactgcgca accagagcgt tcgtgagttt
      541 ctggatggca cccgcaagac caacaatatc ttttgcagta acccgaatca ccgcacgccg
      601 acgctgacca gtatctactg ccgcggctgc agcaaaccac tgtgctgcag ctgtgcgctg
      661 ctggatagca gccacagcga actgaagtgc gacatcagtg cggaaatcca gcaacgccaa
      721 gaagaactcg atgcgatgac ccaagcgctg caagaacaag atagtgcgtt tggtgccgtt
      781 catgcccaga tgcatgcggc ggttggtcaa ctgggccgtg cccgcgccga aaccgaagaa
      841 ctgatccgtg agcgcgtgcg ccaagttgtg gcccatgttc gcgcccaaga acgcgaactg
      901 ctggaagcgg ttgatgcgcg ctaccagcgt gattatgaag agatggccag ccgtctgggt
      961 cgtctggatg cggtgctgca gcgcattcgc accggtagcg cgctggtgca gcgcatgaaa
     1021 tgctatgcga gcgaccaaga agtgctggat atgcacggtt ttctgcgtca agcgctgtgc
     1081 cgtctgcgcc aagaggaacc acagagtctg caagccgccg tgcgtaccga tggcttcgac
     1141 gaattcaagg ttcgtctgca agatctgagc agctgcatca cccaaggcaa agatgccgcg
     1201 gtgagcaaaa aagcgagccc agaagccgcc agtaccccca gggaccctat tgacgttgac
     1261 ctggatgtct ccaatacaac gacagcccag aagaggaagt gcagccagac ccagtgcccc
     1321 aggaaggtca tcaagatgga gtctgaggag gggaaggagg caaggttggc tcggagctcc
     1381 ccggagcagc ccaggcccag cacctccaag gcagtctcac caccccacct ggatggaccg
     1441 cctagcccca ggagccccgt cataggaagt gaggtcttcc tgcccaacag caaccacgtg
     1501 gccagtggcg ccggggaggc agaggaacgc gttgtggtga tcagcagctc ggaagactca
     1561 gatgccgaaa actcgtgcat ggagcccatg gagaccgccg agccacagtc ctcgccagcc
     1621 cactcctcgc cagcccactc ctcgccagcc cactcctcgc cagtccagtc tctgctgaga
     1681 gcacaaggag cctccagcct gccctgtggc acataccacc ccccagcttg gcctccccac
     1741 cagcccgctg agcaggctgc cacccccgat gctgagcctc acagcgagcc tcctgatcac
     1801 caggagcgcc ctgccgtcca ccgtgggatc cgctacctgt tgtacagagc acagagagcc
     1861 atccgccttc gccatgccct ccgcttgcac cctcaattgc atcgggcccc tattcggact
     1921 tggtctcccc atgtggtcca agccagcact cctgccatca cagggcccct caaccatcct
     1981 gccaatgccc aggaacatcc tgcccagctg caaaggggca tcagcccacc ccaccggata
     2041 cgaggggctg tgcgatcccg cagccgctcc ctccggggct cctcccattt atcccagtgg
     2101 ctcaacaact tttttgccct ccccttctcc tccatggctt cccagcttga catgtcttcc
     2161 gtggtggggg caggggaaag cagagcccag actcttggag caggtgttcc ccctggggac
     2221 tctgtcagag gctccatgga ggcctctcaa gtccaagtgc ctctggaagc ctctccaatt
     2281 acattcccac caccctgtgc cccagaaagg ccccccatca gcccagtccc aggcgcccgt
     2341 caagcaggcc tctagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     2401 tatatatctt gtggaaagga cgatagcatt ctagcaatcc cttgagt
//
